Abstract:
:The red pigments in meat products, including cooked cured ham, arise from the reaction of myoglobin with nitric oxide generated from exogenous nitrite. Since carcinogenic nitrosoamines may be generated by the treatment of meats with nitrite, the production of nitrite-free meat products is an attractive alternative. Raw dry-cured (Parma) hams are produced by the treatment of meats with salts other than nitrite. Analysis of pigments in raw dry-cured hams reveals that the main pigment is zinc protoporphyrin, suggesting that the conversion of heme to zinc protoporphyrin occurs via an iron-removal reaction from myoglobin heme during the processing of raw hams. Purification of the iron-removal enzyme showed that it was identical to ferrochelatase. Recombinant ferrochelatase in combination with NADH-cytochrome b5 reductase catalyzed NADH-dependent iron-removal reaction from hemin and hemoproteins. Metal ions such as zinc and cobalt were also removed from the corresponding metalloporphyrins. The addition of zinc ions led to the formation of zinc protoporphyrin. In cultured cells, the conversion of zinc mesoporphyrin to mesoheme was observed to be dependent on ferrochelatase and could be markedly induced during erythroid differentiation. This is the first demonstration of a new enzyme reaction, the reverse reaction of ferrochelatase, which may contribute to a new route of the recycling of protoporphyrin and heme in cells.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Taketani S,Ishigaki M,Mizutani A,Uebayashi M,Numata M,Ohgari Y,Kitajima Sdoi
10.1021/bi701460xsubject
Has Abstractpub_date
2007-12-25 00:00:00pages
15054-61issue
51eissn
0006-2960issn
1520-4995journal_volume
46pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Cytomegaloviruses (CMVs) are ubiquitous species-specific viruses that establish acute, persistent, and latent infections. Both human and mouse CMVs encode proteins that inhibit the activation of natural killer (NK) cells by downregulating cellular ligands for the NK cell activating receptor, NKG2D. The MCMV glycoprote...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi902130j
更新日期:2010-03-23 00:00:00
abstract::Site-directed mutagenesis and random mutagenesis were used to produce variants of subtilisin BPN' (Bacillus amyloliquefaciens) protease with variable surface adsorption properties. Protease adsorption and peptide hydrolysis rate were measured for these variants using a model substrate consisting of a peptide covalentl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi951990h
更新日期:1996-03-12 00:00:00
abstract::The enzyme endonuclease V initiates repair of deaminated DNA bases by making an endonucleolytic incision on the 3' side one nucleotide from a base lesion. In this study, we have used site-directed mutagenesis to characterize the role of the highly conserved residues D43, E89, D110, and H214 in Thermotoga maritima endo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060512b
更新日期:2006-08-29 00:00:00
abstract::The vanadate cofactor in vanadium chloroperoxidase has been studied using UV-VIS absorption spectroscopy. A band is present in the near-UV that is red-shifted as compared to free vanadate and shifts in both position and intensity upon change in pH. Mutation of vanadate binding residues has a clear effect on the spectr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9921790
更新日期:2000-02-08 00:00:00
abstract::The baker's yeast saccharopine dehydrogenase (EC 1.5.1.7) was inactivated by 2,3-butanedione following pseudo-first-order reaction kinetics. The pseudo-first-order rate constant for inactivation was linearly related to the butanedione concentration, and a value of 7.5 M-1 min-1 was obtained for the second-order rate c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00506a004
更新日期:1981-02-03 00:00:00
abstract::Thermostable direct hemolysin (TDH) of Vibrio parahemolyticus is a membrane-damaging pore-forming toxin with potent cytolytic/cytotoxic activity. TDH exists as a tetramer consisting of protomers with a core β-sandwich domain, flanked by an 11-amino acid long N-terminal region (NTR). This NTR could not be modeled in th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00937
更新日期:2020-02-04 00:00:00
abstract::The binding of the inhibitor 8-nitroxanthine to urate oxidase has been investigated by Raman and UV-visible absorption spectroscopy. The absorption maximum of 8-nitroxanthine shifts from 380 to 400 nm upon binding to the enzyme, demonstrating that the electronic structure of the ligand is perturbed. It has been propos...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0507837
更新日期:2005-08-30 00:00:00
abstract::Three different cleavable photoactivable heterobifunctional reagents have been synthesized and characterized by proton NMR, 13C NMR, mass spectroscopy, infrared spectroscopy, and thin-layer chromatography. They are the N-hydroxysuccinimide ester of 3-[(4-azidophenyl)dithio]-propionic acid, methyl 3-[(4-azidophenyl)dit...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00527a003
更新日期:1981-11-24 00:00:00
abstract::Rapid withdrawal of estrogen from immature chicks, previously stimulated with the hormone, results in the inhibition of transcription of mRNAs of egg white proteins, rapid degradation of existing estrogen-induced mRNAs of egg white proteins, and decline in ribosomes and weight of the oviduct. On rapid withdrawal of es...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00409a019
更新日期:1988-05-03 00:00:00
abstract::Sedimentation equilibrium results, obtained with bovine zinc-free insulin (with and without a component of proinsulin) at pH 7.0, I o.2, 25 degrees C, and up to a total concentration of 0.8 g/l., are shown to be consistent with three different polymerization patterns, all involving an isodesmic indefinite self-associa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00666a018
更新日期:1976-10-19 00:00:00
abstract::Aminopeptidases catalyze the hydrolysis of amino acid residues from the amino terminus of peptide substrates. They are found in most cells and tissues, and their activity has been implicated in myriad fundamental biochemical and physiological processes. Nevertheless, little is known about the structure of the aminopep...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00131a034
更新日期:1992-04-28 00:00:00
abstract::The in vitro reaction of the vinyl chloride metabolite chloroacetaldehyde (CAA) with cytosine and adenine residues in ribo- and deoxyribopolynucleotides leads to the formation of the relatively stable hydrated etheno derivatives 3,N4-(N4-alpha-hydroxyethene)adenine (epsilon C . H2O) and 1,N6-(N6-alpha-hydroxyethene)cy...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00265a050
更新日期:1982-10-26 00:00:00
abstract::ZFY, a male-associated Zn-finger protein encoded by the human Y chromosome, exhibits a distinctive two-finger repeat: whereas odd-numbered domains fit a general consensus, even-numbered domains exhibit systematic differences. Do these odd and even sequences encode structurally distinct surfaces for DNA recognition? As...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00243a005
更新日期:1991-07-23 00:00:00
abstract::Polyphosphate glucokinase from Mycobacterium tuberculosis catalyzes the phosphorylation of glucose using inorganic polyphosphates [poly(P)] or ATP. The steady-state kinetic mechanisms of the poly(P)- and ATP-dependent glucokinase reactions were investigated using initial velocity, product inhibition, and dead-end inhi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9528659
更新日期:1996-07-30 00:00:00
abstract::Studies of the mechanism of protein secretion in a Gram-positive bacterium, Bacillus subtilis, yielded results very similar to those previously obtained with a Gram-negative organism: nascent chains protruding from protoplasts could be labeled extracellularly; the labeled chains could be recovered on polysomes isolate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00568a030
更新日期:1979-01-09 00:00:00
abstract::The major phospholipids, phosphatidylcholine and spingomyelin, of low density lipoprotein (LDL) are accessible to small amounts of Pr3+, suggesting that the head groups of all mobile phospholipids are on the surface of the particle in contact with the aqueous medium. The major source of the nuclear Overhauser effect e...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00607a002
更新日期:1978-07-11 00:00:00
abstract::In order to further characterize the binding of the hybrid molecule NetOPC [bis(pyrrolecarboxamide)-oxazolopyridocarbazole conjugate] to double-stranded DNA, we have performed a molecular modeling study to investigate the binding modes of the complexes possibly formed between NetOPC and synthetic polynucleotides poly ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00172a018
更新日期:1994-02-15 00:00:00
abstract::To identify the specific component(s) in the target membrane involved in fusion of vesicular stomatitis virus (VSV), we examined the interaction of the virus with human erythrocyte membranes with asymmetric and symmetric bilayer distributions of phospholipids. Fusion was monitored spectrofluorometrically by the octade...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00469a005
更新日期:1990-05-01 00:00:00
abstract::We have performed comparative studies of the neutral glycosphingolipids synthesized by three human myeloid leukemia cell lines, K562, KG1, and HL-60, which were metabolically labeled with [14C]galactose, to evaluate changes in neutral glycosphingolipid synthesis with myeloid cell differentiation. Individual neutral gl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00345a034
更新日期:1985-11-19 00:00:00
abstract::We report the use of electrospray ionization (ESI) mass spectrometry (MS) in conjunction with online rapid mixing to monitor the kinetics of acid-induced ferrihemoglobin denaturation. Under equilibrium conditions, the hemoglobin mass spectrum is dominated by the intact heterotetramer. Dimeric and monomeric species are...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048501a
更新日期:2004-11-23 00:00:00
abstract::The membrane receptor for immunoglobulin E (IgE) and its ligand, IgE, were irradiated with high-energy electrons. Loss of binding activity was measured for each, and the size of the functional targets was assessed. In both cases, the target size was substantially smaller than the covalent structure of the molecule. Th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00526a011
更新日期:1981-11-10 00:00:00
abstract::Cold-shock proteins (Csps) are expressed at lower-than-optimum temperatures, and they function as RNA chaperones; however, no structural studies on psychrophilic Csps have been reported. Here, we aimed to investigate the structure and dynamics of the Csp of psychrophile Colwellia psychrerythraea 34H, ( Cp-Csp). Althou...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00144
更新日期:2018-07-03 00:00:00
abstract::The Plasmodium falciparum malarial parasite genome appears to encode one and only one phosphatidylinositol 3'-kinase (PI3K), and sequence analysis suggests that the enzyme is a "class III"- or "Vps34"-type PI3K. PfVps34 has generated excitement as a possible druggable target and potentially a key target of artemisinin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00416
更新日期:2017-08-22 00:00:00
abstract::The Sir2 enzyme family is responsible for a newly classified chemical reaction, NAD(+)-dependent protein deacetylation. New peptide substrates, the reaction mechanism, and the products of the acetyl transfer to NAD(+) are described for SIR2. The final products of SIR2 reactions are the deacetylated peptide and the 2' ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011858j
更新日期:2001-12-25 00:00:00
abstract::Although the binding of various forms of bleomycin to DNA has been studied extensively, the transient nature of the activated bleomycin species which ultimately attacks DNA has largely precluded direct examination of its physical interactions with DNA. In an attempt to map the minimum binding site required for this sp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962957d
更新日期:1997-04-08 00:00:00
abstract::The members of the glutamine amidotransferase (GATase) family catalyze the incorporation of ammonia within numerous metabolic pathways and can be categorized in two classes. Here, we concentrated on class I GATases, which are heteromeric enzyme complexes consisting of synthase subunits and glutaminase subunits with a ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00286
更新日期:2019-06-04 00:00:00
abstract::The Haemophilus influenzae diaminopimelate epimerase was cloned, expressed, purified, and crystallized in the C2221 space group (a = 102.1 A, b = 115.4 A, c = 66.3 A, alpha = beta = gamma = 90 degrees). The three-dimensional structure was solved to 2.7 A using a single Pt derivative and the Se-Met-substituted enzyme t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi982138o
更新日期:1998-11-24 00:00:00
abstract::The carboxy-terminal domain of the alpha subunit of Escherichia coli RNA polymerase, which is connected with the core part of RNA polymerase through a long flexible linker, plays decisive roles in transcription activation by directly interacting with a large number of transcription factors and upstream (UP) element DN...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000020d
更新日期:2000-05-23 00:00:00
abstract::Mutation of Pro94 to phenylalanine or alanine significantly alters the redox properties of the type I copper center of amicyanin. Each mutation increases the redox midpoint potential (E(m)) value by at least 140 mV and shifts the pK(a) for the pH dependence of the E(m) value to a more acidic value. Atomic resolution (...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049634z
更新日期:2004-07-27 00:00:00
abstract::In bacteria, regulatory phosphorylation of proteins at serine and/or threonine residues by Ser/Thr protein kinase (STPK) is an emerging theme in prokaryotic signaling, particularly since the prediction of the occurrence of several STPKs from genome sequencing and sequence surveys. Here we show that protein PknH posses...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035150b
更新日期:2003-12-30 00:00:00