Abstract:
:Picosecond absorption spectroscopy has been used to investigate energy-transfer dynamics within the LH1 and LH2 light-harvesting complexes of three mutants of Rhodobacter sphaeroides. We demonstrate that both complexes are inhomogeneous; each contains a specialized pigment pool which absorbs maximally at a longer wavelength. Within LH2 (mutant NF57), Bchl850 transfers energy to Bchl870 in 39 +/- 9 ps; within LH1 (mutants M21 and M2192), energy is transferred from Bchl875 to Bchl896 in 22 +/- 4 and 35 +/- 5 ps, respectively. Examination of the decay of induced absorption anisotropy indicates that each of these specialized pools exists in a state which is highly organized with respect to the remainder of the pigments. Such an arrangement may facilitate and direct energy migration toward the reaction center.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Hunter CN,Bergström H,van Grondelle R,Sundström Vdoi
10.1021/bi00465a008subject
Has Abstractpub_date
1990-04-03 00:00:00pages
3203-7issue
13eissn
0006-2960issn
1520-4995journal_volume
29pub_type
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