Crystal structure and ligand-binding studies of a screened peptide complexed with streptavidin.

abstract：:The N-terminal extracellular parts of human G-protein coupled receptor class B, for example, receptors for secretin, glucagon, or parathyroid hormone, are involved in ligand binding. To obtain structural and functional information on the N-terminal receptor fragment of human parathyroid hormone receptor 1 (PTHR1), the...

journal_title：Biochemistry

authors： Grauschopf U,Lilie H,Honold K,Wozny M,Reusch D,Esswein A,Schäfer W,Rücknagel KP,Rudolph R

更新日期：2000-08-01 00:00:00

abstract：:We have studied the effect of the binding of ribosomal protein S1 and initiation factor IF3 on the accessibility of nucleotide residues 584-1506 in the small subunit of the Escherichia coli ribosome. Protein S1 strongly decreases RNase V1 attack at G1164, in hairpin 40 of the 3' major domain, and weakly decreases DMS ...

journal_title：Biochemistry

authors： Laughrea M,Tam J

更新日期：1991-12-03 00:00:00

abstract：:The solution structure of the complementary d(C1-A2-T3-G4-X5-G6-T7-A8-C9).d(G10-T11-A12-C13-A14-C15-A16-T17-G18) DNA duplex (designated X.A 9-mer), which contains a 1,N2-propanodeoxyguanosine exocyclic adduct X5 opposite deoxyadenosine A14 at the center, is pH dependent [Kouchakdjian, M., Eisenberg, M., Live, D., Mari...

journal_title：Biochemistry

authors： Huang P,Patel DJ,Eisenberg M

更新日期：1993-04-20 00:00:00

abstract：:PKC phosphorylation regulates PITX2 DNA binding and transcriptional activity. Mutation of individual PKC sites demonstrates the functional regulation of PITX2 through phosphorylation. Immunoprecipitation of PITX2 and a PITX2 PKC mutant protein reveal specific in vivo phosphorylation by PKC in transfected cells. The tr...

journal_title：Biochemistry

authors： Espinoza HM,Ganga M,Vadlamudi U,Martin DM,Brooks BP,Semina EV,Murray JC,Amendt BA

更新日期：2005-03-15 00:00:00

abstract：:Cysteine dioxygenase (CDO) is a non-heme monoiron enzyme with an unusual posttranslational modification in the proximity of the ferrous iron active site. This modification, a cysteine to tyrosine thioether bond, cross-links two β-strands of the β-barrel. We have investigated its role in catalysis through a combined cr...

journal_title：Biochemistry

authors： Davies CG,Fellner M,Tchesnokov EP,Wilbanks SM,Jameson GN

更新日期：2014-12-23 00:00:00

abstract：:The incorporation of extensively fluorinated, or fluorous, analogues of hydrophobic amino acids into proteins potentially provides the opportunity to modulate the physicochemical properties of proteins in a predictable manner. On the basis of the properties of small fluorocarbon molecules, extensively fluorinated prot...

journal_title：Biochemistry

authors： Buer BC,de la Salud-Bea R,Al Hashimi HM,Marsh EN

更新日期：2009-11-17 00:00:00

abstract：:The function of Lys-59, Arg-141, and Arg-277 in PAPS binding and catalysis of the flavonol 3-sulfotransferase was investigated. Affinity chromatography of conservative mutants with PAPS analogues allowed us to determine that Lys-59 interacts with the 5' portion of the nucleotide, while Arg-141 interacts with the 3' po...

journal_title：Biochemistry

authors： Marsolais F,Laviolette M,Kakuta Y,Negishi M,Pedersen LC,Auger M,Varin L

更新日期：1999-03-30 00:00:00

abstract：:Regulation of adenylate cyclase by alpha 2 adrenergic receptors requires the inhibitory guanine nucleotide binding protein Ni. A role for this protein has also been suggested in the high-affinity binding of agonists to the alpha 2 receptor. We recently reported that alkaline treatment can selectively inactivate alpha ...

journal_title：Biochemistry

authors： Kim MH,Neubig RR

更新日期：1987-06-16 00:00:00

abstract：:Cytochrome c has been chemically modified by methylene blue mediated photooxidation. It is established that the methionine residues of the protein have been specifically converted to methionine sulfoxide residues. No oxidation of any other amino acid residues or the cysteine thioether bridges of the molecule occurs du...

journal_title：Biochemistry

authors： Ivanetich KM,Bradshaw JJ,Kaminsky LS

更新日期：1976-03-09 00:00:00

abstract：:There has been intense interest in the development of factor Xa inhibitors for the treatment of thrombotic diseases. Our laboratory has developed a series of novel non-amidine inhibitors of factor Xa. This paper presents two crystal structures of compounds from this series bound to factor Xa. The first structure is de...

journal_title：Biochemistry

authors： Adler M,Kochanny MJ,Ye B,Rumennik G,Light DR,Biancalana S,Whitlow M

更新日期：2002-12-31 00:00:00

abstract：:The photoreceptor protein rhodopsin has been reconstituted with a single phospholipid species, dimyristoylphosphatidylcholine, at a range of different lipid/protein ratios, and the exchange rate at the lipid-protein interface has been determined from the electron spin resonance spectra of spin-labeled phosphatidylchol...

journal_title：Biochemistry

authors： Ryba NJ,Horváth LI,Watts A,Marsh D

更新日期：1987-06-02 00:00:00

abstract：:Lipid species changes for SV40-transformed fibroblasts from wild-type or from diacylglycerol kinase-epsilon (DGKepsilon) or diacylglycerol kinase-alpha (DGKalpha) knockout mice were determined for glycerophospholipids, polyphosphatidylinositides (GPInsP n ) and diacylglycerol (DAG) using direct infusion mass spectrome...

journal_title：Biochemistry

authors： Milne SB,Ivanova PT,Armstrong MD,Myers DS,Lubarda J,Shulga YV,Topham MK,Brown HA,Epand RM

更新日期：2008-09-09 00:00:00

abstract：:Earlier work indicates that the major DNA repair phosphodiesterase (PDE) in yeast cells is the well-characterized Apn1 protein. Apn1 demonstrates both Mg2+-independent PDE activity and Mg2+-independent class II apurinic/apyrimidinic (AP) endonuclease activity and represents greater than 90% of the activity detected in...

journal_title：Biochemistry

authors： Sander M,Ramotar D

更新日期：1997-05-20 00:00:00

abstract：:Gelatinase B is a matrix metalloproteinase (MMP-9) involved in tissue remodeling, development, cancer, and inflammation. Neutrophils produce three major forms of (pro)gelatinase B: 92 kDa monomers, homodimers, and complexes of gelatinase B covalently bound to neutrophil gelatinase B-associated lipocalin (NGAL). In con...

journal_title：Biochemistry

authors： Rudd PM,Mattu TS,Masure S,Bratt T,Van den Steen PE,Wormald MR,Küster B,Harvey DJ,Borregaard N,Van Damme J,Dwek RA,Opdenakker G

更新日期：1999-10-19 00:00:00

abstract：:The question of the possible identity of catalytic and regulatory proton pathways in the chloroplast FoF1 ATPase has been studied using different energy-transfer inhibitors. Venturicidin, a reversible inhibitor of Fo, affects neither the delta mu H(+)-dependent thiol reduction of the membrane-bound chloroplast ATPase ...

journal_title：Biochemistry

authors： Valerio M,de Kouchkovsky Y,Haraux F

更新日期：1992-05-05 00:00:00

abstract：:Out of over 60 single-Cys mutants in putative periplasmic loops in lactose permease, three mutants [Tyr101 --> Cys (loop III/IV), Leu313 --> Cys (loop IX/X), and Ser375 --> Cys (loop XI/XII)] spontaneously form disulfide-linked dimers, indicating that these loops are located on the periphery of the 12-helix bundle tha...

journal_title：Biochemistry

authors： Sun J,Kaback HR

更新日期：1997-09-30 00:00:00

abstract：:Nitric oxide is produced in mammals by the nitric oxide synthase (NOS) isoforms at a catalytic site comprising a heme associated with a biopterin cofactor. Through genome sequencing, proteins that are highly homologous to the oxygenase domain of NOSs have been identified, in particular in bacteria. The active site is ...

journal_title：Biochemistry

authors： Weisslocker-Schaetzel M,Lembrouk M,Santolini J,Dorlet P

更新日期：2017-02-07 00:00:00

abstract：:The flexibility of individual amino acid side chains of pancreatic phospholipase A2 in aqueous and micellar solutions was studied with deuterium nuclear magnetic resonance (2H NMR). Bovine pancreatic phospholipase A2 was selectively deuterated at the aromatic ring systems of Trp-3 and Phe-5 and porcine pancreatic phos...

journal_title：Biochemistry

authors： Allegrini PR,van Scharrenburg GJ,Slotboom AJ,de Haas GH,Seelig J

更新日期：1985-06-18 00:00:00

abstract：:IIIGlc is an 18.1-kDa signal-transducing phosphocarrier protein of the phosphoenolpyruvate:glycose phosphotransferase system (PTS) of Escherichia coli. Virtually complete (98%) backbone 1H, 15N, and 13C nuclear magnetic resonance (NMR) signal assignments were determined by using a battery of triple-resonance three-dim...

journal_title：Biochemistry

authors： Pelton JG,Torchia DA,Meadow ND,Wong CY,Roseman S

更新日期：1991-10-15 00:00:00

abstract：:Human DJ-1, a disease-associated protein that protects cells from oxidative stress, contains an oxidation-sensitive cysteine (C106) that is essential for its cytoprotective activity. The origin of C106 reactivity is obscure, due in part to the absence of an experimentally determined p K a value for this residue. We ha...

journal_title：Biochemistry

authors： Witt AC,Lakshminarasimhan M,Remington BC,Hasim S,Pozharski E,Wilson MA

更新日期：2008-07-15 00:00:00

abstract：:The channel-forming protein OmpF porin from Escherichia coli spans the bacterial outer membrane. Each of the three monomers comprises a hollow, 16-stranded beta-barrel. These are associated to homotrimers which are unusually stable, due mostly to hydrophobic interactions between the beta-barrels. In addition, a loop, ...

journal_title：Biochemistry

authors： Phale PS,Philippsen A,Kiefhaber T,Koebnik R,Phale VP,Schirmer T,Rosenbusch JP

更新日期：1998-11-10 00:00:00

abstract：:Human lung tryptase (HLT), a trypsin-like serine proteinase stored as an active enzyme in association with heparin in mast cell granules, is released into the extracellular environment when mast cells are activated. Tryptases are unusual in that they form tetramers and bind heparin. As there are no known endogenous tr...

journal_title：Biochemistry

authors： Addington AK,Johnson DA

更新日期：1996-10-22 00:00:00

abstract：:Escherichia coli DNA photolyase contains a stable flavin radical and a second chromophore (SC) of unknown structure. The effects of flash (both conventional and laser) excitation of either the radical alone or both the radical and the second chromophore have been investigated by variation of the excitation wavelengths...

journal_title：Biochemistry

authors： Heelis PF,Payne G,Sancar A

更新日期：1987-07-28 00:00:00

abstract：:Peripheral benzodiazepine receptors (PBR), first described more than 20 years ago, have been attributed with many putative functions including ones in cellular proliferation and cellular respiration. Hence, it is quite conceivable that deregulation of this receptor could lead to pathology. We and others have reported ...

journal_title：Biochemistry

authors： Weisinger G,Kelly-Hershkovitz E,Veenman L,Spanier I,Leschiner S,Gavish M

更新日期：2004-09-28 00:00:00

abstract：:Homology modeling was used to identify two particular residues, Glu175 and Ala176, in Pseudomonas stutzeri phosphite dehydrogenase (PTDH) as the principal determinants of nicotinamide cofactor (NAD(+) and NADP(+)) specificity. Replacement of these two residues by site-directed mutagenesis with Ala175 and Arg176 both s...

journal_title：Biochemistry

authors： Woodyer R,van der Donk WA,Zhao H

更新日期：2003-10-14 00:00:00

abstract：:Strong inward-rectifier K(+) (Kir) channels play a significant role in shaping the cardiac action potential: they help produce its long plateau and accelerate its rate of repolarization. Consequently, genetic deletion of the gene encoding the strongly rectifying K(+) channel IRK1 (Kir2.1) prolongs the cardiac action p...

journal_title：Biochemistry

authors： Xu Y,Lu Z

更新日期：2004-12-14 00:00:00

abstract：:The only identified component of the scrapie prion is PrPSc, a glycosylinositol phospholipid (GPI)-linked protein that is derived from the cellular isoform (PrPC) by an as yet unknown posttranslational event. Analysis of the PrPSc GPI has revealed six different glycoforms, three of which are unprecedented. Two of the ...

journal_title：Biochemistry

authors： Stahl N,Baldwin MA,Hecker R,Pan KM,Burlingame AL,Prusiner SB

更新日期：1992-06-02 00:00:00

abstract：:The reaction of cytochrome c oxidase (COX) from Rhodobacter sphaeroides with hydrogen peroxide has been studied at alkaline (pH 8.5) and acidic (pH 6.5) conditions with the aid of a stopped-flow apparatus. Absorption changes in the entire 350-800 nm spectral range were monitored and analyzed by a global fitting proced...

journal_title：Biochemistry

authors： Pecoraro C,Gennis RB,Vygodina TV,Konstantinov AA

更新日期：2001-08-14 00:00:00

abstract：:The mechanism of chromophore biosynthesis in green fluorescent protein (GFP) is triggered by a spontaneous main chain cyclization reaction of residues 65-67. Here, we demonstrate that the initially colorless Y66L variant, designed to trap chromophore precursor states, is oxidatively modified to generate yellow chromop...

journal_title：Biochemistry

authors： Rosenow MA,Patel HN,Wachter RM

更新日期：2005-06-14 00:00:00

abstract：:2-Azidoadenosine 5'-diphosphate (2-azido-ADP) labeled with 32P in the alpha-position was prepared and used to photolabel the nucleotide binding sites of beef heart mitochondrial F1-ATPase. The native F1 prepared by the procedure of Knowles and Penefsky [Knowles, A. F., & Penefsky, H. S. (1972) J. Biol. Chem. 247, 6617...

journal_title：Biochemistry

authors： Boulay F,Dalbon P,Vignais PV

更新日期：1985-12-03 00:00:00