Stability of trimeric OmpF porin: the contributions of the latching loop L2.

abstract：:The EP3 subtype of prostaglandin E2 receptor transduces diverse physiological responses in mammalian tissues through signaling pathways coupled to heterotrimeric G proteins. Distinct cDNA clones encoding five isoforms of the EP3 receptor were isolated from a human uterus cDNA library. The human EP3 receptor isoforms d...

journal_title：Biochemistry

authors： An S,Yang J,So SW,Zeng L,Goetzl EJ

更新日期：1994-12-06 00:00:00

abstract：:Runx proteins constitute a family of mammalian transcription factors that interact with DNA through their evolutionarily conserved Runt domain. CBFbeta, alternatively denoted PEBP2beta, is the non-DNA-binding heterodimer partner and acts to enhance the DNA binding affinity of Runx proteins. Runx proteins and CBFbeta a...

journal_title：Biochemistry

authors： Wolf-Watz M,Grundström T,Härd T

更新日期：2001-09-25 00:00:00

abstract：:ArsR (or ArsR/SmtB) family metalloregulatory homodimeric repressors collectively respond to a wide range of metal ion inducers in regulating homeostasis and resistance of essential and nonessential metal ions in bacteria. BxmR from the cyanobacterium Osciliatoria brevis is the first characterized ArsR protein that sen...

journal_title：Biochemistry

authors： Liu T,Chen X,Ma Z,Shokes J,Hemmingsen L,Scott RA,Giedroc DP

更新日期：2008-10-07 00:00:00

abstract：:Epidermal growth factor receptor (EGFR) exemplifies the family of receptor tyrosine kinases that mediate numerous cellular processes, including growth, proliferation, and differentiation. Moreover, gene amplification and EGFR mutations have been identified in a number of human malignancies, making this receptor an imp...

journal_title：Biochemistry

authors： Truong TH,Carroll KS

更新日期：2012-12-18 00:00:00

abstract：:G protein-coupled receptor (GPCR) kinases (GRKs) instigate the desensitization of activated GPCRs via phosphorylation that promotes interaction with arrestins, thereby preventing the interaction of GPCRs with heterotrimeric G proteins. A current proposed model of GRK1 activation involves the binding of activated rhodo...

journal_title：Biochemistry

authors： Orban T,Huang CC,Homan KT,Jastrzebska B,Tesmer JJ,Palczewski K

更新日期：2012-04-24 00:00:00

abstract：:Incorporation of nonproteinogenic amino acids in small polypeptides synthesized by nonribosomal peptide synthetases (NRPS) significantly contributes to their biological activity. In these peptides, conversion of L-amino acids to the corresponding D-isomer is catalyzed by specialized NRPS modules that utilize an epimer...

journal_title：Biochemistry

authors： Linne U,Doekel S,Marahiel MA

更新日期：2001-12-25 00:00:00

abstract：:Mitochondria isolated from coenzyme Q deficient yeast cells had no detectable NADH:cytochrome c reductase or succinate:cytochrome c reductase but had comparable amounts of cytochromes b and c1 as wild-type mitochondria. Addition of succinate to the mutant mitochondria resulted in a slight reduction of cytochrome b; ho...

journal_title：Biochemistry

authors： Clejan L,Beattie DS

更新日期：1986-12-02 00:00:00

abstract：:Treatment of ADP-glucose pyrophosphorylase (EC 2.7.7.27) from the cyanobacterium Anabaena PCC 7120 with phenylglyoxal in 50 mM Hepes, pH 8.0, at 25 degrees C resulted in a time- and concentration-dependent loss of enzyme activity. Phosphate, the inhibitor, protected the enzyme from inactivation most effectively, while...

journal_title：Biochemistry

authors： Sheng J,Preiss J

更新日期：1997-10-21 00:00:00

abstract：:Human apolipoprotein stimulation of sphingomyelin (SM) hydrolysis by sphingomyelinase from human skin fibroblasts has been studied. Apolipoproteins A-I, A-II, B, C-I, and E do not enhance sphingomyelin hydrolysis above control levels. In contrast, apoC-II stimulates sphingomyelin hydrolysis by approximately 2.5-fold. ...

journal_title：Biochemistry

authors： Ahmad TY,Beaudet AL,Sparrow JT,Morrisett JD

更新日期：1986-07-29 00:00:00

abstract：:Hydrophobic interaction of 8-anilino-1-naphthalene sulfonic acid (ANS) with proteins is one of the widely used methods for characterizing/detecting partially folded states of proteins. We have carried out a systematic investigation on the effect of ANS, a charged hydrophobic fluorescent dye, on structural properties o...

journal_title：Biochemistry

authors： Ali V,Prakash K,Kulkarni S,Ahmad A,Madhusudan KP,Bhakuni V

更新日期：1999-10-12 00:00:00

abstract：:Single-stranded microRNAs (miRNAs) regulate gene expression by triggering mRNA degradation and/or inhibiting mRNA translation. miRNAs play important roles in various critical cellular processes and are associated with numerous human diseases, including cancer and neurodegenerative diseases. miRNA sequences are embedde...

journal_title：Biochemistry

authors： Nguyen HM,Nguyen TD,Nguyen TL,Nguyen TA

更新日期：2019-01-29 00:00:00

abstract：:West Nile virus (WNV) is a mosquito-borne flavivirus with a rapidly expanding global distribution. Infection can cause severe neurological disease and fatality in humans. Efforts are ongoing to develop antiviral drugs that inhibit the WNV protease, a viral enzyme required for polyprotein processing. Unfortunately, lit...

journal_title：Biochemistry

authors： Garces AP,Watowich SJ

更新日期：2013-10-01 00:00:00

abstract：:The structure of [14-38]Abu, a variant of bovine pancreatic trypsin inhibitor (BPTI) with only the 14-38 disulfide bridge intact, has been analyzed by two-dimensional 1H and 1H-15N NMR. Except for the 18-24, 29-35 antiparallel beta-sheet, residues in all regions of the molecule give two exchange cross peaks for each 1...

journal_title：Biochemistry

authors： Barbar E,Barany G,Woodward C

更新日期：1995-09-12 00:00:00

abstract：:The structures of four isomorphous crystals of ternary complexes of chicken heart citrate synthase with D- or L-malate and acetyl coenzyme A or carboxymethyl coenzyme A have been determined by X-ray crystallography and fully refined at 1.9-A resolution. The structures show that both L-malate and D-malate bind in a ver...

journal_title：Biochemistry

authors： Karpusas M,Holland D,Remington SJ

更新日期：1991-06-18 00:00:00

abstract：:We present a study of the interactions between the lipase from Thermomyces lanuginosus (TlL) and the two microbially produced biosurfactants (BSs), rhamnolipid (RL) and sophorolipid (SL). Both RL and SL are glycolipids; however, RL is anionic, while SL is a mixture of anionic and non-ionic species. We investigate the ...

journal_title：Biochemistry

authors： Madsen JK,Kaspersen JD,Andersen CB,Nedergaard Pedersen J,Andersen KK,Pedersen JS,Otzen DE

更新日期：2017-08-15 00:00:00

abstract：:The binding of the codon UUC to the isolated anticodon loop of tRNAPhe (yeast) has been studied as a model of codon recognition by a simple adaptor. Fluorescence titrations demonstrate that UUC binds to the isolated anticodon loop with an equilibrium constant of 1.4 X 10(3) M-1 (at 7.2 degrees C). Equilibrium sediment...

journal_title：Biochemistry

authors： Bujalowski W,Jung M,McLaughlin LW,Porschke D

更新日期：1986-10-21 00:00:00

abstract：:Fragment-based approaches to finding novel small molecules that bind to proteins are now firmly established in drug discovery and chemical biology. Initially developed primarily in a few centers in the biotech and pharma industry, this methodology has now been adopted widely in both the pharmaceutical industry and aca...

journal_title：Biochemistry

authors： Scott DE,Coyne AG,Hudson SA,Abell C

更新日期：2012-06-26 00:00:00

abstract：:The structure of the copper site in oxidized and reduced Rhus vernicifera stellacyanin has been studied by X-ray absorption (XAFS) spectroscopy at different pH values. Data for the oxidized protein are consistent with the fourth ligand being an O- or N-donating ligand rather than a cysteine from the disulfide bridge. ...

journal_title：Biochemistry

authors： Strange RW,Reinhammar B,Murphy LM,Hasnain SS

更新日期：1995-01-10 00:00:00

abstract：:The molecular structure of ammonium deoxycytidylyl-(3'-5')-deoxyguanosine, crystallized from aqueous acetone near pH 4, was determined for X-ray diffraction data. The crystals were tetragonal, space group P43212 with a = b = 11.078 (1) A and c = 45.826 (4) A. The structure was solved by tangent expansion of phases bas...

journal_title：Biochemistry

authors： Cruse WB,Egert E,Kennard O,Sala GB,Salisbury SA,Viswamitra MA

更新日期：1983-04-12 00:00:00

abstract：:p-Cresol methylhydroxylase (PCMH) isolated from Pseudomonas putida is an alpha 2 beta 2 tetramer of approximate subunit Mr 49,000 and 9,000. It is a flavocytochrome c containing covalently bound FAD in the larger subunit and covalently bound heme in the smaller. Crystals in space group P2(1)2(1)2(1) with unit-cell par...

journal_title：Biochemistry

authors： Mathews FS,Chen ZW,Bellamy HD,McIntire WS

更新日期：1991-01-08 00:00:00

abstract：:Zinc, the catalytically essential metal of angiotensin converting enzyme (ACE), has been replaced by cobalt(II) to give an active, chromophoric enzyme that is spectroscopically responsive to inhibitor binding. Visible absorption spectroscopy and magnetic circular dichroic spectropolarimetry have been used to character...

journal_title：Biochemistry

authors： Bicknell R,Holmquist B,Lee FS,Martin MT,Riordan JF

更新日期：1987-11-17 00:00:00

abstract：:Bacillus thuringiensis (Bt) insecticidal toxins bind to receptors on midgut epithelial cells of susceptible insects, and binding triggers biochemical events that lead to insect mortality. Recently, a 100-kDa aminopeptidase N (APN) was isolated from brush border membrane vesicles (BBMV) of Anopheles quadrimaculatus and...

journal_title：Biochemistry

authors： Zhang R,Hua G,Andacht TM,Adang MJ

更新日期：2008-10-28 00:00:00

abstract：:Urease from Klebsiella aerogenes [Jabri et al. (1995) Science 268, 998-1004] is an (alpha beta gamma)3 trimer with each alpha-subunit having an (alpha beta)8-barrel domain containing a binickel active center. Here we examine structure-function relations for urease in more detail through structural analysis of the urea...

journal_title：Biochemistry

authors： Jabri E,Karplus PA

更新日期：1996-08-20 00:00:00

abstract：:PHD2 is a 2-oxoglutarate, non-heme Fe(2+)-dependent oxygenase that senses O2 levels in human cells by hydroxylating two prolyl residues in the oxygen-dependent degradation domain (ODD) of HIF1α. Identifying the active site contacts that determine the rate of reaction at limiting O2 concentrations is crucial for unders...

journal_title：Biochemistry

authors： Pektas S,Taabazuing CY,Knapp MJ

更新日期：2015-05-12 00:00:00

abstract：:Pectate lyase C (pelC) was the first protein in which the parallel beta-helix structure was recognized. The unique features of parallel beta-helix-containing proteins-a relatively simple topology and unusual interactions among side chains-make pelC an interesting protein to study with respect to protein folding. In th...

journal_title：Biochemistry

authors： Kamen DE,Griko Y,Woody RW

更新日期：2000-12-26 00:00:00

abstract：:Two hybridomas producing monoclonal antibodies to poly(adenosine diphosphate ribose) [poly(ADP-Rib)] were established. One antibody, 10H (IgG3, kappa), bound to most of the poly(ADP-Rib) preparation, which consisted of molecules of various sizes of more than 20 ADP-Rib residues. The binding of this antibody was inhibi...

journal_title：Biochemistry

authors： Kawamitsu H,Hoshino H,Okada H,Miwa M,Momoi H,Sugimura T

更新日期：1984-07-31 00:00:00

abstract：:The distance between the calcium site (site I) and the zinc site (site II) in alpha-lactalbumin was estimated from Forster energy-transfer measurements between donor Eu(III) [or Tb(III)] at site I and acceptor Co(II) at site II to be 11.5 +/- 1.5 A. Intersite distances were also measured between the bis-ANS [4,4'-bis[...

journal_title：Biochemistry

authors： Musci G,Berliner LJ

更新日期：1986-08-26 00:00:00

abstract：:The reversible inhibition of beta-lactamase I from Bacillus cereus by cloxacillin, methicillin, and nafcillin has been systematically investigated. For these substrates the enzymatic reaction involves partitioning of the substrate between turnover and inhibition. Typically, concentrations of several hundred millimolar...

journal_title：Biochemistry

authors： Fink AL,Behner KM,Tan AK

更新日期：1987-07-14 00:00:00

abstract：:Molecular cloning revealed the unique serpin endopin 2C that demonstrates selective inhibition of cathepsin L compared to papain or elastase. Endopin 2C, thus, functions as a serpin with the property of cross-class inhibition. Endopin 2C possesses homology in primary sequence to endopin 2A and other isoforms of endopi...

journal_title：Biochemistry

authors： Hwang SR,Stoka V,Turk V,Hook VY

更新日期：2005-05-31 00:00:00

abstract：:Time-resolved spectroscopic studies of unfolded horse iron(II) cytochrome c have suggested that the imidazole side chains of His26 and His33 bind transiently to the heme iron on microsecond time scales, after photodissociation of a carbon monoxide ligand from the heme. Our studies of four variants of cytochrome c (hor...

journal_title：Biochemistry

authors： Hagen SJ,Latypov RF,Dolgikh DA,Roder H

更新日期：2002-01-29 00:00:00