Abstract:
:Two hybridomas producing monoclonal antibodies to poly(adenosine diphosphate ribose) [poly(ADP-Rib)] were established. One antibody, 10H (IgG3, kappa), bound to most of the poly(ADP-Rib) preparation, which consisted of molecules of various sizes of more than 20 ADP-Rib residues. The binding of this antibody was inhibited by not only poly-(ADP-Rib) but also a monomer unit of poly(ADP-Rib), Ado(P)-Rib-P. The sites protected by antibody 10H were isolated and analyzed by hydrolysis with alkaline phosphomonoesterase and then snake venom phosphodiesterase. The sites contained the same amounts of monomer units and branched portions [Ado(P)-Rib(P)-Rib-P] as the original poly(ADP-Rib) molecules but a lower average number of branched portions per molecule than in the original molecules. The other antibody, 16B (IgM, lambda), reacted with only 50% of the radioactive poly(ADP-Rib), and its binding was not inhibited by a monomer unit. This antibody protected 25% of all the poly(ADP-Rib) molecules from hydrolysis by snake venom phosphodiesterase. The protected sites contained twice as many branched portions per molecule as the original poly(ADP-Rib) molecules. These results show that the two monoclonal antibodies recognize different structures of poly-(ADP-Rib); 10H antibody recognizes the linear structure with ribose-ribose linkages, and 16B antibody may recognize specific structures, including the branched portions of poly-(ADP-Rib).
journal_name
Biochemistryjournal_title
Biochemistryauthors
Kawamitsu H,Hoshino H,Okada H,Miwa M,Momoi H,Sugimura Tdoi
10.1021/bi00311a032subject
Has Abstractpub_date
1984-07-31 00:00:00pages
3771-7issue
16eissn
0006-2960issn
1520-4995journal_volume
23pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Paranemic crossover DNA (PX-DNA) is a four-stranded multicrossover structure that has been implicated in recombination-independent recognition of homology. Although existing evidence has suggested that PX is the DNA motif in homologous pairing (HP), this conclusion remains ambiguous. Further investigation is needed bu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00057
更新日期:2019-03-12 00:00:00
abstract::Hepatitis C virus (HCV) protease NS3 and its protein activator NS4A participate in the processing of the viral polyprotein into its constituent nonstructural proteins. The NS3/4A complex is thus an attractive target for antiviral therapy against HCV. We expressed the full-length NS3 and NS4A in insect cells as a solub...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972010r
更新日期:1998-03-10 00:00:00
abstract::Using heteronuclear NMR spectroscopy, we demonstrate that a 13-residue peptide (MS-QIKRLLSEKKT) from the cytoplasmic tail of CD4 binds to Nef protein. This part of CD4 is critical for downregulation of CD4 by HIV-1 Nef [Aiken et al. (1994) Cell 76, 853-864]. We show that a control peptide without the central dileucine...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9611164
更新日期:1996-08-13 00:00:00
abstract::The following polynucleotides containing the antibiotic tubercidin (Tu; 4-amino-7-beta-D-ribofuranosylpyrrolo-[2,3-d]pyrimidine) were enzymatically synthesized by polymerization of adenosine 5'-diphosphate-tubercidin 5'-diphosphate mixtures with polynucleotide phosphorylase: poly(A2,Tu), poly(A,Tu2), and poly(Tu). The...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00512a030
更新日期:1981-04-28 00:00:00
abstract::The pyridoxal 5'-phosphate dependent histidine decarboxylase from Morganella morganii shows a nitrogen isotope effect k14/k15 = 0.9770 +/- 0.0021, a carbon isotope effect k12/k13 = 1.0308 +/- 0.0006, and a carbon isotope effect for L-[alpha-2H]histidine of 1.0333 +/- 0.0001 at pH 6.3, 37 degrees C. These results indic...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00416a016
更新日期:1988-08-09 00:00:00
abstract::A detailed structural study of the prokaryotic sodium/galactose transporter (vSGLT) from Vibrio parahaemolyticus using attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy reveals stepwise increases in alpha-helicity upon binding of sodium and D-galactose. These increases in helicity correlat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi025692d
更新日期:2002-06-25 00:00:00
abstract::Two synthetic peptides corresponding to sequences in HIV-1LAI gp41, (aa558-595) and T20 (aa 643-678), are strong inhibitors of HIV-1 viral fusion, having EC50 values of 1 microgram/mL and 1 ng/mL, respectively. Previous work suggested that T21 forms a coiled-coil structure in PBS solution, while T20 is primarily nonhe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9606962
更新日期:1996-10-22 00:00:00
abstract::The first transmembrane (TM1) helix in the red cell anion exchanger (AE1, Band 3, or SLC4A1) acts as an internal signal anchor that binds the signal recognition particle and directs the nascent polypeptide chain to the endoplasmic reticulum (ER) membrane where it moves from the translocon laterally into the lipid bila...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00966
更新日期:2017-02-07 00:00:00
abstract::We examine the relationship between binding affinity and interface size for reversible protein-protein interactions (PPIs), using cytokines from the tumor necrosis factor (TNF) superfamily and their receptors as a test case. Using surface plasmon resonance, we measured single-site binding affinities for binding of the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi301039t
更新日期:2012-11-13 00:00:00
abstract::Protein phosphatase-1 (PP1) is an essential protein Ser/Thr phosphatase that is extraordinarily conserved from yeast to human, and Inhibitor-2 (I-2) is the most ancient of the heat-stable proteins specific for PP1. We identified novel I-2 homologues in Caenorhabditis elegans (Ce) and Xenopus laevis (Xe) and compared t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi602369m
更新日期:2007-03-06 00:00:00
abstract::(1) The rate of ATP synthesis during NADH-driven aerobic respiration has been measured in plasma membrane vesicles from Paracoccus denitrificans as a function of the concentration of the substrates, ADP and inorganic phosphate (Pi). In both cases, the response of the reaction to changes in the degree of saturation of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00498a013
更新日期:1990-11-20 00:00:00
abstract::Phosphite dehydrogenase (PTDH) is a unique NAD-dependent enzyme that catalyzes the oxidation of inorganic phosphite to phosphate. The enzyme has great potential for cofactor regeneration, and mechanistic studies have provided some insight into the residues that are important for catalysis. In this investigation, pre-s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701550c
更新日期:2007-11-13 00:00:00
abstract::We previously showed that beta-amyloid precursor protein (APP) is cleaved not only in the middle of the membrane (gamma-cleavage) but also at novel cleavage sites close to the membrane/cytoplasmic boundary (epsilon-cleavage), releasing APP intracellular domains (AICDs) 49-99 and 50-99. To learn more about the relation...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049399k
更新日期:2004-10-26 00:00:00
abstract::We have investigated the action of the human DNA polymerase ε (hpol ε) and η (hpol η) catalytic cores on G-quadruplex (G4) DNA substrates derived from the promoter of the c-MYC proto-oncogene. The translesion enzyme hpol η exhibits a 6.2-fold preference for binding to G4 DNA over non-G4 DNA, while hpol ε binds both G4...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00060
更新日期:2015-05-26 00:00:00
abstract::Beta(2)-microglobulin (beta(2)m) forms amyloid fibrils that deposit in the musculo-skeletal system in patients undergoing long-term hemodialysis. How beta(2)m self-assembles in vivo is not understood, since the monomeric wild-type protein is incapable of forming fibrils in isolation in vitro at neutral pH, while elong...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi052434i
更新日期:2006-02-21 00:00:00
abstract::To identify interactions between Ste2p, a G protein-coupled receptor of the yeast Saccharomyces cerevisiae, and its tridecapeptide ligand, alpha-factor (WHWLQLKPGQPMY), a variety of alpha-factor analogues were used in conjunction with site-directed mutagenesis of a targeted portion of Ste2p transmembrane domain six. A...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026100u
更新日期:2002-11-19 00:00:00
abstract::The molecular weight of phosphophoryn, an acidic phosphoprotein unique to dentin matrix, has been difficult to determine because of a combination of neutral protease activities in this tissue and the intrinsic high charge density of the molecule. In this study, bovine dentin phosphophoryn (BDPP) was isolated by a proc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00287a025
更新日期:1983-08-30 00:00:00
abstract::Ner repressors of the transposable phages Mu and D108 play a central role in regulating the expression of the early (transposase) operon and in ensuring that phage growth proceeds along a lytic pathway. The latter function is analogous to that performed by the Cro protein of phage lambda. Unlike lambda Cro, however, t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00201a018
更新日期:1994-09-06 00:00:00
abstract::Fibronectin (FN) is an extracellular matrix (ECM) protein found soluble in corporal fluids or as an insoluble fibrillar component incorporated in the ECM. This phenomenon implicates structural changes that expose FN binding sites and activate the protein to promote intermolecular interactions with other FN. We have in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0347104
更新日期:2004-02-17 00:00:00
abstract::The enzyme form of the calcium adenosinetriphosphatase of sarcoplasmic reticulum (CaATPase) that is stable in the presence of calcium, cE.Ca2, has a binding site for the catalytic Mg2+ ion with a dissociation constant of 0.94 +/- 0.15 mM at 25 degrees C, pH 7.0, and 100 mM KCl. This is approximately 10 times smaller t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00077a016
更新日期:1993-07-06 00:00:00
abstract::The fluorescence of the base analogue 2-aminopurine (2AP) was used to probe bacteriophage T4 DNA polymerase-induced conformational changes in the template strand produced during the nucleotide incorporation and proofreading reactions. 2AP fluorescence in DNA is quenched by 2AP interactions with neighboring bases, but ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051462y
更新日期:2005-12-06 00:00:00
abstract::Clip domains are structural modules found in arthropod serine proteinases and some proteolytically inactive homologues, which mediate extracellular signaling pathways of development and immunity. While little is known about their structures or functions, clip domains are proposed to be sites for interactions of protei...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi7010724
更新日期:2007-10-16 00:00:00
abstract::The modulatory action of Ca2+-calmodulin on multiple targets is inhibited by trifluoperazine, which competes with target proteins for calmodulin binding. The structure of calmodulin crystallized with two trifluoperazine molecules is determined by X-ray crystallography at 2.74 A resolution. The X-ray data together with...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980795a
更新日期:1998-11-03 00:00:00
abstract::Peptidylglycine monooxygenase (PHM) catalyzes the final step in the biosynthesis of amidated peptides that serve as important signaling molecules in numerous endocrine pathways. The catalytic mechanism has attracted much attention because of a number of unique attributes, including the presence of a pair of uncoupled ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi4015264
更新日期:2014-02-18 00:00:00
abstract::A cationic, copper(II)-containing ligand, derived from bulky 5,10,15,20-tetrakis(N-methylpyridinium-4-yl)porphyrin, Cu(T4), and two sterically friendlier forms, [trans-5,15-di(N-methylpyridinium-4-yl)porphyrinato]copper(II), Cu(tD4), and [cis-5,10-di(N-methylpyridinium-4-yl)porphyrinato]copper(II), Cu(cD4), bind to DN...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300828z
更新日期:2012-09-25 00:00:00
abstract::Neuronal specification is regulated by the activity of transcription factors containing the basic helix-loop-helix motif (bHLH); these regulating proteins include, among others, the neurogenin (Ngn) family, related to the atonal family of genes. Neurogenin 1 (NGN1) is a 237-residue protein that contains a bHLH domain ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901616z
更新日期:2010-03-02 00:00:00
abstract::Monomeric sarcosine oxidase (MSOX) contains covalently bound FAD and catalyzes the oxidation of sarcosine (N-methylglycine) and other secondary amino acids, including L-proline. The reductive half-reaction with L-proline proceeds via a rapidly attained equilibrium (K(d)) between free E(ox) and the E(ox).S complex, fol...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi020285n
更新日期:2002-08-06 00:00:00
abstract::The Ca2+-dependent, reversible, interaction of cyclic adenosine 3',5'-monophosphate (cAMP) phosphodiesterase with its activator has been used to purify the enzyme by affinity chromatography. Activator-dependent cAMP phosphodiesterase is only a minor component of the proteins specifically adsorbed in the presence of Ca...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00594a017
更新日期:1978-01-10 00:00:00
abstract::The inhibition mechanism of serpins requires a change in structure to entrap the target proteinase as a stable acyl-enzyme complex. Although it has generally been assumed that reactive center loop insertion and associated conformational change proceeds in a concerted manner, this has not been demonstrated directly. Th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi025967p
更新日期:2002-10-08 00:00:00
abstract::Hemoglobins extracted from fishes that live in temperate waters show little or no dissociation even in the liganded form, unlike human hemoglobin (HbA). To establish whether cold adaptation influences the tendency to dissociate, the dimer-tetramer association constants (L(2,4)) of the carbonmonoxy derivatives of repre...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002297j
更新日期:2001-03-13 00:00:00