当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Effect of the Southeast Asian Ovalocytosis Deletion on the Conformational Dynamics of Signal-Anchor Transmembrane Segment 1 of Red Cell Anion Exchanger 1 (AE1, Band 3, or SLC4A1).

Effect of the Southeast Asian Ovalocytosis Deletion on the Conformational Dynamics of Signal-Anchor Transmembrane Segment 1 of Red Cell Anion Exchanger 1 (AE1, Band 3, or SLC4A1).

Abstract：

:The first transmembrane (TM1) helix in the red cell anion exchanger (AE1, Band 3, or SLC4A1) acts as an internal signal anchor that binds the signal recognition particle and directs the nascent polypeptide chain to the endoplasmic reticulum (ER) membrane where it moves from the translocon laterally into the lipid bilayer. The sequence N-terminal to TM1 forms an amphipathic helix that lies at the membrane interface and is connected to TM1 by a bend at Pro403. Southeast Asian ovalocytosis (SAO) is a red cell abnormality caused by a nine-amino acid deletion (Ala400-Ala408) at the N-terminus of TM1. Here we demonstrate, by extensive (∼4.5 μs) molecular dynamics simulations of TM1 in a model 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine membrane, that the isolated TM1 peptide is highly dynamic and samples the structure of TM1 seen in the crystal structure of the membrane domain of AE1. The SAO deletion not only removes the proline-induced bend but also causes a "pulling in" of the part of the amphipathic helix into the hydrophobic phase of the bilayer, as well as the C-terminal of the peptide. The dynamics of the SAO peptide very infrequently resembles the structure of TM1 in AE1, demonstrating the disruptive effect the SAO deletion has on AE1 folding. These results provide a precise molecular view of the disposition and dynamics of wild-type and SAO TM1 in a lipid bilayer, an important early biosynthetic intermediate in the insertion of AE1 into the ER membrane, and extend earlier results of cell-free translation experiments.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Fowler PW,Sansom MS,Reithmeier RA

doi

10.1021/acs.biochem.6b00966

subject

Has Abstract

pub_date

2017-02-07 00:00:00

pages

712-722

issue

5

eissn

0006-2960

issn

1520-4995

journal_volume

56

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Molecular Determinants for Unphosphorylated STAT3 Dimerization Determined by Integrative Modeling.

    abstract：:Signal transducer and activator of transcription factors (STATs) are proteins that can translocate into the nucleus, bind DNA, and activate gene transcription. STAT proteins play a crucial role in cell proliferation, apoptosis, and differentiation. The prevalent view is that STAT proteins are able to form dimers and b...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi501529x

    authors： Sgrignani J,Olsson S,Ekonomiuk D,Genini D,Krause R,Catapano CV,Cavalli A

    更新日期：2015-09-08 00:00:00

  • Phosphorylation of the gamma subunit of the retinal photoreceptor cGMP phosphodiesterase by the cAMP-dependent protein kinase and its effect on the gamma subunit interaction with other proteins.

    abstract：:Cyclic GMP phosphodiesterase, a key enzyme in phototransduction, is composed of P alpha beta and two P gamma subunits. Interaction of P gamma with P alpha beta or with the alpha subunit (T alpha) of transducin is crucial for the regulation of cGMP phosphodiesterase in retinal photoreceptors. Here we have investigated ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi973087i

    authors： Xu LX,Tanaka Y,Bonderenko VA,Matsuura I,Matsumoto H,Yamazaki A,Hayashi F

    更新日期：1998-04-28 00:00:00

  • Nuclear deoxyribonucleic acid characterization of the marine chromophyte Olisthodiscus luteus.

    abstract：:Nuclear DNA of the marine chromophytic alga Olisthodiscus luteus was analyzed in this study. Reassociation kinetics analysis has shown that 440-nucleotide DNA fragments from the genome of this alga contain 4% foldback, 58% repetitive, and 34% single-copy sequences. Precise analysis using isolated single-copy DNA revea...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00527a023

    authors： Ersland DR,Cattolico RA

    更新日期：1981-11-24 00:00:00

  • Maduropeptin: an antitumor chromoprotein with selective protease activity and DNA cleaving properties.

    abstract：:Maduropeptin (MDP) is a recently isolated antitumor antibiotic, consisting of an enediyne-containing chromophore embedded in a highly acidic protein. This holoantibiotic damages duplex DNA in vitro, producing a mixture of single- and double-strand breaks at selected sites. The chromophore, isolated as the methanol add...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00036a035

    authors： Zein N,Solomon W,Colson KL,Schroeder DR

    更新日期：1995-09-12 00:00:00

  • Inactivation of factor VIII by factor IXa.

    abstract：:Factor VIII (FVIII) is the nonproteolytic cofactor for FIXa in the tenase complex of blood coagulation. FVIII is proteolytically activated by thrombin and FXa in vitro to form a heterotrimer with full procoagulant activity. Activated protein C inactivates thrombin-activated FVIII through cleavage adjacent to position ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00125a022

    authors： O'Brien DP,Johnson D,Byfield P,Tuddenham EG

    更新日期：1992-03-17 00:00:00

  • Effects of metal ligation and oxygen on the reversibility of the thermal denaturation of Pseudomonas aeruginosa azurin.

    abstract：:Thermodynamic equilibrium transition models in DSC are only applicable to reversible processes, but reversibility of the thermal transitions of proteins is comparatively rare because of intermolecular aggregation of denatured proteins and the degradation that occurs at high temperatures. The cupredoxin azurin from Pse...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0157621

    authors： Sandberg A,Leckner J,Shi Y,Schwarz FP,Karlsson BG

    更新日期：2002-01-22 00:00:00

  • Targeted cross-linking of a molten globule form of acetylcholinesterase by the virucidal agent hypericin.

    abstract：:The natural product hypericin is a photosensitive polycyclic aromatic dione compound, which has been widely investigated because of its virucidal and antitumor properties. Although it has been suggested that singlet oxygen or a radical species might be responsible for its biological action, its mechanism of action rem...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi991147+

    authors： Weiner L,Roth E,Mazur Y,Silman I

    更新日期：1999-08-31 00:00:00

  • Exchange of histidine spacing between Sp1 and GLI zinc fingers: distinct effect of histidine spacing-linker region on DNA binding.

    abstract：:In the DNA recognition mode of C(2)H(2)-type zinc fingers, the finger-finger connection region, consisting of the histidine spacing (HX(3-5)H) and linker, would be important for determining the orientation of the zinc finger domains. To clarify the influence of spacing between two ligand histidines in the DNA binding,...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi035999s

    authors： Shiraishi Y,Imanishi M,Sugiura Y

    更新日期：2004-05-25 00:00:00

  • Thermodynamics of reversible and irreversible unfolding and domain interactions of glucoamylase from Aspergillus niger studied by differential scanning and isothermal titration calorimetry.

    abstract：:The stability of three forms of glucoamylase from Aspergillus niger has been investigated by differential scanning and isothermal titration calorimetry: Glucoamylase 1 (GA1), which consists of a catalytic domain and a starch-binding domain (SBD) connected by a heavily O-glycosylated linker region; glucoamylase 2 (GA2)...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990185q

    authors： Christensen T,Svensson B,Sigurskjold BW

    更新日期：1999-05-11 00:00:00

  • An inhibitor of ribosomal peptidyl transferase using transition-state analogy.

    abstract：:The phosphoramidate of CCdAp and puromycin (CCdApPuro) is a potent inhibitor of ribosomal peptidyl transferase, as assayed by the fragment reaction. Inhibition is competitive at the ribosomal A-site. CCdApPuro protects P-site-associated bases in the peptidyl transferase loop region of 23S rRNA from carbodiimide modifi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00002a001

    authors： Welch M,Chastang J,Yarus M

    更新日期：1995-01-17 00:00:00

  • Structural interactions dictate the kinetics of macrophage migration inhibitory factor inhibition by different cancer-preventive isothiocyanates.

    abstract：:Regulation of cellular processes by dietary nutrients is known to affect the likelihood of cancer development. One class of cancer-preventive nutrients, isothiocyanates (ITCs), derived from the consumption of cruciferous vegetables, is known to have various effects on cellular biochemistry. One target of ITCs is macro...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi3005494

    authors： Crichlow GV,Fan C,Keeler C,Hodsdon M,Lolis EJ

    更新日期：2012-09-25 00:00:00

  • ATP cross-linked to Escherichia coli single-strand DNA-binding protein can be utilized by the catalytic center of primase as initiating nucleotide for primer RNA synthesis on phage G4oric template.

    abstract：:We report a new observation of the role of Escherichia coli single-strand DNA binding protein (SSB) in synthesis of primer RNA (pRNA) catalyzed by.E.coli primase on the SSB-coated phage G4oric template. Using a set of ATP priming substrates with reactive groups attached to the 5' gamma-phosphate on different length "a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi972455f

    authors： Godson GN,Mustaev AA,Sun W

    更新日期：1998-03-17 00:00:00

  • Structural composition of canine secretory component and immunoglobulin A.

    abstract：:Dog serum and colostral immunoglobulin A (IgA) and free secretory component from colostrum were isolated using affinity chromatography. Both serum and colostral IgA showed similar susceptibility to reduction with dithiothreitol, but only colostral IgA released the additional subunit, bound secretory component. This re...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00684a010

    authors： Thompson RE,Reynolds HY,Waxdal MJ

    更新日期：1975-07-01 00:00:00

  • Cardiac troponin T forms a tetramer in vitro.

    abstract：:Cardiac troponin T (cTnT) is a myofibrillar protein essential for calcium-dependent contraction. This property has led to functional studies of developmentally expressed cTnT isoforms and mutants identified in patients with hypertrophic cardiomyopathy. The release of cTnT into the serum following myocardial infarction...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi7012596

    authors： Lounes KC,Demeler B,Anderson DE,Gomes AW,Potter JD,Nassar R,Anderson PA

    更新日期：2008-02-19 00:00:00

  • Substrate binding to the peripheral site of acetylcholinesterase initiates enzymatic catalysis. Substrate inhibition arises as a secondary effect.

    abstract：:Two sites of ligand interaction in acetylcholinesterase (AChE) were first demonstrated in ligand binding studies and later confirmed by crystallography, site-specific mutagenesis, and molecular modeling: an acylation site at the base of the active site gorge and a peripheral site at its mouth. We recently introduced a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9813577

    authors： Szegletes T,Mallender WD,Thomas PJ,Rosenberry TL

    更新日期：1999-01-05 00:00:00

  • Using 2-aminopurine fluorescence to detect bacteriophage T4 DNA polymerase-DNA complexes that are important for primer extension and proofreading reactions.

    abstract：:The fluorescence of the base analogue 2-aminopurine (2AP) was used to probe bacteriophage T4 DNA polymerase-induced conformational changes in the template strand produced during the nucleotide incorporation and proofreading reactions. 2AP fluorescence in DNA is quenched by 2AP interactions with neighboring bases, but ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi051462y

    authors： Hariharan C,Reha-Krantz LJ

    更新日期：2005-12-06 00:00:00

  • Kinetic analysis of NodST sulfotransferase using an electrospray ionization mass spectrometry assay.

    abstract：:A novel and efficient enzyme kinetics assay using electrospray ionization mass spectrometry was developed and applied to the bacterial carbohydrate sulfotransferase (NodST). NodST catalyzes the sulfuryl group transfer from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to chitobiose, generating 3'-phosphoadenosine 5'-ph...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi020457g

    authors： Pi N,Armstrong JI,Bertozzi CR,Leary JA

    更新日期：2002-11-05 00:00:00

  • A ParE-ParC fusion protein is a functional topoisomerase.

    abstract：:Type II topoisomerases are responsible for DNA unlinking during DNA replication and chromosome segregation. Although eukaryotic enzymes are homodimers and prokaryotic enzymes are heterotetramers, both prokaryotic and eukaryotic type II topoisomerases belong to a single protein family. The amino- and carboxyl-terminal ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0155201

    authors： Lavasani LS,Hiasa H

    更新日期：2001-07-24 00:00:00

  • Characterization of wild-type and an active-site mutant in Escherichia coli of short-chain acyl-CoA dehydrogenase from Megasphaera elsdenii.

    abstract：:The objective of this work is to determine the molecular mechanism and regulation of short-chain acyl-CoA dehydrogenase (SCAD) from Megasphaera elsdenii. To achieve this, the gene coding for SCAD from M. elsdenii was cloned and sequenced. Site-directed mutagenesis was then used to identify an amino acid residue that i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00091a026

    authors： Becker DF,Fuchs JA,Banfield DK,Funk WD,MacGillivray RT,Stankovich MT

    更新日期：1993-10-12 00:00:00

  • Inhibitors bound to Ca(2+)-free sarcoplasmic reticulum Ca(2+)-ATPase lock its transmembrane region but not necessarily its cytosolic region, revealing the flexibility of the loops connecting transmembrane and cytosolic domains.

    abstract：:Ca2+-free crystals of sarcoplasmic reticulum Ca2+-ATPase have, up until now, been obtained in the presence of inhibitors such as thapsigargin (TG), bound to the transmembrane region of this protein. Here, we examined the consequences of such binding for the protein. We found that, after TG binding, an active site liga...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi701855r

    authors： Montigny C,Picard M,Lenoir G,Gauron C,Toyoshima C,Champeil P

    更新日期：2007-12-25 00:00:00

  • Librational motion of an "immobilized" spin label: hemoglobin spin labeled by a maleimide derivative.

    abstract：:The spin label Tempo-maleimide, when "immobilized" in hemoglobin, is shown to exhibit motional fluctuation whose amplitude and/or frequency depend on temperature and solution conditions. These motional fluctuations are observable by several electron spin resonance techniques. For desalted hemoglobin the fluctuations a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00600a014

    authors： Johnson ME

    更新日期：1978-04-04 00:00:00

  • Evidence for unique homologous peptide sequences around the glycosylated seryl and threonyl residues in polysialoglycoproteins isolated from the unfertilized eggs of the Pacific salmon Oncorhynchus keta.

    abstract：:Structures of glycopeptides obtained by exhaustive Pronase digestion of high molecular weight (1.7 X 10(5)) salmon egg polysialoglycoprotein have been elucidated. Six principal glycopeptides isolated by gel chromatography and DEAE-Sephadex A-25 chromatography in the absence or presence of borate ion were analyzed for ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00341a029

    authors： Shimamura M,Inoue Y,Inoue S

    更新日期：1985-09-24 00:00:00

  • Factor IX activation by factor XIa proceeds without release of a free intermediate.

    abstract：:Factor IX activation by factor XIa is thought to proceed through the singly-cleaved free intermediate, factor IX alpha. However, we observed no intermediate development during factor IX activation by factor XIa when using a low substrate to enzyme ratio (44:1 mol/mol). This result can be explained by one of two mechan...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi962274y

    authors： Wolberg AS,Morris DP,Stafford DW

    更新日期：1997-04-08 00:00:00

  • Characterization of ribonucleic acid polymerase-T7 promoter binary complexes.

    abstract：:Several experimental approaches have been employed to independently determine rates of dissociation for Escherichia coli RNA polymerase from the T7 bacteriophage A1, A2, A3, and D promoters. Heparin challenge measurements employing abortive initiation turnover rates as an index of promoter occupancy were carried out o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00552a023

    authors： Cech CL,McClure WR

    更新日期：1980-05-27 00:00:00

  • Ion Channel Formation by Tau Protein: Implications for Alzheimer's Disease and Tauopathies.

    abstract：:Tau is a microtubule associated protein implicated in the pathogenesis of several neurodegenerative diseases. Because of the channel forming properties of other amyloid peptides, we employed planar lipid bilayers and atomic force microscopy to test tau for its ability to form ion permeable channels. Our results demons...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00988

    authors： Patel N,Ramachandran S,Azimov R,Kagan BL,Lal R

    更新日期：2015-12-22 00:00:00

  • Transducer-binding and transducer-mutations modulate photoactive-site-deprotonation in sensory rhodopsin I.

    abstract：:Sensory rhodopsin I (SRI) is a seven-transmembrane helix retinylidene protein that mediates color-sensitive phototaxis responses through its bound transducer HtrI in the archaeon Halobacterium salinarum. Deprotonation of the Schiff base attachment site of the chromophore accompanies formation of the SRI signaling stat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi991180w

    authors： Jung KH,Spudich EN,Dag P,Spudich JL

    更新日期：1999-10-05 00:00:00

  • Improvement by benzoquinones of the quantum yield of photoactivation of photosynthetic oxygen evolution: direct evidence for the two-quantum mechanism.

    abstract：:Effects of eight differently substituted 1,4-benzoquinones (BQs) on the quantum yield of photoactivation of oxygen evolution (reconstitution of the Mn cluster) were examined with wheat photosystem II (PSII) membranes depleted of the Mn cluster by treatment with 1.0 mM NH2OH. Illumination with 10 flashes at 0.25-s inte...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00117a032

    authors： Miyao-Tokutomi M,Inoue Y

    更新日期：1992-01-21 00:00:00

  • Binding of Clostridium perfringens [125I]enterotoxin to rabbit intestinal cells.

    abstract：:125I-Labeled enterotoxin from Clostridium perfringens was utilized to characterize the association of the enterotoxin with cells isolated from rabbit intestine and tissue homogenates from liver, kidney, and brain. The enterotoxin was found to bind in a specific and saturable manner to cells from intestine and to tissu...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00562a014

    authors： McDonel JL

    更新日期：1980-10-14 00:00:00

  • Characterization of the extracellular ligand-binding domain of the type II activin receptor.

    abstract：:The binding of a ligand to cell surface receptors initiates a cascade of intracellular signals that generate responses to the external stimuli. Thus, this event plays a pivotal role in the mechanism of transmembrane signaling. Activin is a member of a cytokine family that is involved in diverse biological processes. T...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi981939o

    authors： Greenwald J,Le V,Corrigan A,Fischer W,Komives E,Vale W,Choe S

    更新日期：1998-11-24 00:00:00

  • X-ray structure of the magnesium(II).ADP.vanadate complex of the Dictyostelium discoideum myosin motor domain to 1.9 A resolution.

    abstract：:The structure of the vanadate-trapped ADP complex of a truncated head of Dictyostelium myosin II consisting of residues Asp 2-Asn 762 has been determined by molecular replacement at 1.9 A resolution and refined to a crystallographic R-factor of 19.4%. The crystals belong to the orthorhombic space group C2221 where a =...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi952633+

    authors： Smith CA,Rayment I

    更新日期：1996-04-30 00:00:00