Abstract:
:Several experimental approaches have been employed to independently determine rates of dissociation for Escherichia coli RNA polymerase from the T7 bacteriophage A1, A2, A3, and D promoters. Heparin challenge measurements employing abortive initiation turnover rates as an index of promoter occupancy were carried out over a range of heparin concentrations in order to separate intrinsic dissociation rates from dissociation due to direct heparin attack of the polymerase-promoter complex. Dissociation rates were found to vary widely even among major promoters considered to have equivalent strength in vivo. Direct heparin attack was found to occur slowly with respect to intrinsic dissociation of polymerase from most promoters. Results were verified by gel analysis of full-length transcripts after heparin challenge and by measuring abortive initiation rates after poly[d(A-T)] . poly[d(A-T)] challenge. In the latter case, it was discovered that the equilibrium distribution of polymerase between a promoter and poly[d(A-T)] . poly[d(A-T)] could be measured.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Cech CL,McClure WRdoi
10.1021/bi00552a023subject
Has Abstractpub_date
1980-05-27 00:00:00pages
2440-7issue
11eissn
0006-2960issn
1520-4995journal_volume
19pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Pantothenate kinase (PanK) catalyzes the first step of the universal five-step coenzyme A (CoA) biosynthetic pathway. The recently characterized type III PanK (PanK-III, encoded by the coaX gene) is distinct in sequence, structure and enzymatic properties from both the long-known bacterial type I PanK (PanK-I, exempli...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi7018578
更新日期:2008-02-05 00:00:00
abstract::To contribute to the understanding of membrane protein function upon application of pressure as relevant for understanding, for example, the physiology of deep sea organisms or for baroenzymological biotechnical processes, we investigated the influence of hydrostatic pressure on the activity of Na+,K+-ATPase enriched ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi062235e
更新日期:2007-02-13 00:00:00
abstract::Melanoma growth stimulatory activity (MGSA) is a mitogenic protein secreted by Hs294T melanoma cells that corresponds to the polypeptide encoded by the human gro gene. The MGSA/gro cDNA has been expressed in mammalian cells and the secreted recombinant factor has been purified. Biochemical and biological characterizat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00496a011
更新日期:1990-11-06 00:00:00
abstract::Phosphoenolpyruvate carboxykinase (PEPCK) is an essential metabolic enzyme operating in the gluconeogenesis and glyceroneogenesis pathways. Previous work has demonstrated that the enzyme cycles between a catalytically inactive open state and a catalytically active closed state. The transition of the enzyme between the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b01215
更新日期:2016-01-26 00:00:00
abstract::Site-specifically modified oligodeoxynucleotides were used to explore the influence of neighboring base sequence context on the mutagenic potential of N-(deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-AAF) and N-(deoxyguanosin-8-yl)-2-aminofluorene (dG-AF) in mammalian cells. Oligodeoxynucleotides ((5)(')TCCTCCTNXNCTCT...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0027581
更新日期:2001-03-27 00:00:00
abstract::Growth factor receptor-bound protein 14 (Grb14) is involved in growth factor receptor tyrosine kinase signaling. Here we report that light causes a major redistribution of Grb14 among the individual subcellular compartments of the retinal rod photoreceptor. Grb14 is localized predominantly to the inner segment, nuclea...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9000062
更新日期:2009-06-23 00:00:00
abstract::Two classes of bacteriophages, the single-stranded DNA Microviridae and the single-stranded RNA Alloleviviridae, accomplish lysis by expressing "protein antibiotics", or polypeptides that inhibit cell wall biosynthesis. Previously, we have provided genetic and physiological evidence that E, a 91-amino acid membrane pr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900469g
更新日期:2009-06-09 00:00:00
abstract::Thrombin-activatable fibrinolysis inhibitor (TAFI) circulates as an inactive proenzyme of a carboxypeptidase B-like enzyme (TAFIa). It functions by removing C-terminal lysine residues from partially degraded fibrin that are important in tissue-type plasminogen activator mediated plasmin formation. TAFI was classified ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0115683
更新日期:2002-01-29 00:00:00
abstract::The class D β-lactamase OXA-143 has been described as an efficient penicillinase, oxacillinase, and carbapenemase. The D224A variant, known as OXA-231, was described in 2012 as exhibiting less activity toward imipenem and increased oxacillinase activity. Additionally, the P227S mutation was reported as a case of conve...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00365
更新日期:2019-08-27 00:00:00
abstract::The interaction between the beta 2 subunit of tryptophan synthase and the coenzyme pyridoxal 5'-phosphate (PLP) is characterized by induced circular dichroism (CD) in the near-UV (260-285 nm) and in the visible region (320-480 nm, extrinsic Cotton effect). Because of its high mean residue ellipticity ([theta] = 56 deg...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00525a017
更新日期:1981-10-27 00:00:00
abstract::The modified Tanford-Kirkwood electrostatic theory (Shire et al., 1974a) was applied to ferrimyoglobins from the following animal species: sperm whale (Physeter catodon), horse, California grey whale (Eschrichtius gibbosus), harbor seal (Phoca vitulina), and California sea lion (Zalophus californianus). Computations w...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00678a002
更新日期:1975-04-08 00:00:00
abstract::Bacteriophage P22 is a double-stranded DNA containing phage. Its morphogenetic pathway requires the formation of a precursor procapsid that subsequently matures to the capsid. The stability of bacteriophage P22 coat protein in both monomeric and polymeric forms under hydrostatic pressure has been examined previously [...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00004a003
更新日期:1995-01-31 00:00:00
abstract::Human erythrocyte spectrin prepared from fresh blood is a mixture of different association states. Depending on the manner of preparation, the two-chain dimer or the tetramer predominates. These forms are not in rapid thermodynamic equilibrium. The molecular weight of the dimer by sedimentation and diffusion and by li...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00644a028
更新日期:1977-12-13 00:00:00
abstract::The Na(+)-pumping NADH:quinone oxidoreductase (Na(+)-NQR) is a fundamental enzyme of the oxidative phosphorylation metabolism and ionic homeostasis in several pathogenic and marine bacteria. To understand the mechanism that couples electron transfer with sodium translocation in Na(+)-NQR, the ion dependence of the red...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300343u
更新日期:2012-05-15 00:00:00
abstract::Earlier thermodynamic studies of the intermolecular interactions between mature oxytocin and neurophysin, and of the effects of these interactions on neurophysin folding, raised questions about the intramolecular interactions of oxytocin with neurophysin within their common precursor. To address this issue, the disulf...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9912950
更新日期:1999-10-12 00:00:00
abstract::In Saccharomyces cerevisiae, the histidine-containing phosphotransfer (HPt) protein YPD1 transfers phosphoryl groups between the three different response regulator domains of SLN1, SSK1, and SKN7 (designated R1, R2, and R3, respectively). Together these proteins form a branched histidine-aspartic acid phosphorelay sys...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048433s
更新日期:2005-01-11 00:00:00
abstract::Phosphorescence from the lone tryptophan residue has been studied to monitor the interaction of myelin basic protein with phosphatidylserine vesicles. Spectral shifts in the phosphorescence of the protein in a glycerol-buffer (70:30 w/w) solvent at low temperature are consistent with fluorescence data obtained under a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00514a019
更新日期:1981-05-26 00:00:00
abstract::The N-acyl- l-homoserine lactone hydrolases (AHL lactonases) have attracted considerable attention because of their ability to quench AHL-mediated quorum-sensing pathways in Gram-negative bacteria and because of their relation to other enzymes in the metallo-beta-lactamase superfamily. To elucidate the detailed cataly...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi8003704
更新日期:2008-07-22 00:00:00
abstract::Two paramagnetic probes, viz., Mn2+ and Cr3+-ATP, were used to map distances to various loci on carbamoyl-phosphate synthetase by using NMR measurements. The paramagnetic influence of Mn2+ on the 1H of L-glutamate and L-ornithine was measured at 200 and 360 MHz. On the basis of these data, a correlation time for the p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00277a020
更新日期:1983-04-12 00:00:00
abstract::NADH:ubiquinone oxidoreductase (complex I) from bovine heart mitochondria is a highly complicated, energy transducing, membrane-bound enzyme. It contains 46 different subunits and nine redox cofactors: a noncovalently bound flavin mononucleotide and eight iron-sulfur clusters. The mechanism of complex I is not known. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051809x
更新日期:2006-01-10 00:00:00
abstract::We have investigated the role of the labile terminal domains of the core histones on the stability of the subunits of the protein core of the nucleosome by studying the thermodynamic behavior of the products of limited trypsin digestion of these subunits. The thermal stabilities of the truncated H2A-H2B dimer and the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0110140
更新日期:2001-10-30 00:00:00
abstract::Carbonic anhydrase (CA) catalyzes the reversible hydration of carbon dioxide to hydrogen carbonate, and its role in maintaining pH balance has made it an attractive drug target. Steroidal sulfamate esters, inhibitors of the cancer drug target steroid sulfatase (STS), are sequestered in vivo by CA II in red blood cells...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi902178w
更新日期:2010-04-27 00:00:00
abstract::The cystic fibrosis transmembrane conductance regulator forms an anion-selective channel. We previously showed that charge selectivity, the ability to discriminate between anions and cations, occurs near the cytoplasmic end of the channel. The molecular determinants of charge selectivity, however, are unknown. We inve...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990155n
更新日期:1999-04-27 00:00:00
abstract::The mutants Arg54-->Leu, Arg54-->Lys, Arg305-->Lys, Asp309-->Glu, and Trp317-->Phe, located at subsites 1 and 2 in glucoamylase from Aspergillus niger, provide insight into the importance of specific hydrogen bonds and hydrophobic interactions in substrate recognition, catalytic mechanism, and stability. As suggested ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00032a009
更新日期:1995-08-15 00:00:00
abstract::The effect of DnaB helicase on the initiation specificity of primase was studied biochemically using a series of single-stranded DNA templates in which each nucleotide of the trinucleotide d(CTG) initiation sequence was systematically varied. DnaB helicase accelerated the rate of primer syntheisis, prevented "overlong...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi991555d
更新日期:2000-02-01 00:00:00
abstract::EL222 is a blue light sensor protein, which consists of a light-oxygen-voltage domain as a light sensor and a LuxR-type helix-turn-helix DNA-binding domain. The reaction dynamics of the protein-DNA binding were observed for the first time using the time-resolved transient grating method. The reaction scheme was determ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b01206
更新日期:2018-03-13 00:00:00
abstract::To probe the mechanism of chaperone substrate selection, we have investigated the kinetics of complex formation and dissociation between the molecular chaperone DnaK and a short peptide (Cro, representing amino acids 1-12 of the cro repressor protein). The Cro protein was N-terminally labeled with the environmentally ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00047a024
更新日期:1995-11-28 00:00:00
abstract::Hexose-1-phosphate uridylyltransferase catalyzes the interconversion of UDP-galactose and glucose-1-P with UDP-glucose and galactose-1-P by a double-displacement mechanism through a covalent intermediate (E-UMP), in which UMP is bonded to one of two histidine residues at the active site, H164 or H166. To identify whic...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00499a003
更新日期:1990-11-27 00:00:00
abstract::The Met16Phe mutant of the type 1 copper protein pseudoazurin (PACu), in which a phenyl ring is introduced close to the imidazole moiety of the His81 ligand, has been characterized. NMR studies indicate that the introduced phenyl ring is parallel to the imidazole group of His81. The mutation has a subtle effect on the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi030030p
更新日期:2003-06-10 00:00:00
abstract::DrrA and DrrB proteins form an ATP-dependent efflux pump for doxorubicin and daunorubicin in Streptomyces peucetius. DrrA, the catalytic subunit, forms a complex with the integral membrane protein DrrB. Previous studies have provided evidence for strong interaction between these two proteins, which was found to be cri...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701699a
更新日期:2008-03-04 00:00:00