Abstract:
:The binding of a ligand to cell surface receptors initiates a cascade of intracellular signals that generate responses to the external stimuli. Thus, this event plays a pivotal role in the mechanism of transmembrane signaling. Activin is a member of a cytokine family that is involved in diverse biological processes. To study the structural basis that underlies the transmembrane signaling mechanism, we have overexpressed the soluble extracellular domain of the type II activin receptor from mouse (ActRII-ECD). We used the methylotrophic yeast Pichia pastoris as an expression host to produce a large quantity of ActRII-ECD. Expression was carried out in a fermentor with a typical yield of 10 mg of pure ActRII-ECD from a liter of growth media. Biological function was confirmed by the ability to decrease the activin-stimulated release of FSH from cultured rat pituitary cells in addition to several activin-binding assays, including native gel shift and chemical cross-linking. The glycosylation on ActRII-ECD was shown to be dispensable for high-affinity activin binding, and nonnatural sugars from the yeast expression host did not interfere with binding, indicating that the binding of activin is not sensitive to the environment near the two positions of N-linked glycosylation. Analytical ultracentrifugation of the complex between activin A and ActRII-ECD reveals that two receptors associate with one activin A dimer, consistent with results from chemical cross-linking experiments.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Greenwald J,Le V,Corrigan A,Fischer W,Komives E,Vale W,Choe Sdoi
10.1021/bi981939osubject
Has Abstractpub_date
1998-11-24 00:00:00pages
16711-8issue
47eissn
0006-2960issn
1520-4995pii
bi981939ojournal_volume
37pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The flavin-dependent quiescin-sulfhydryl oxidase (QSOX) inserts disulfide bridges into unfolded reduced proteins with the reduction of molecular oxygen to form hydrogen peroxide. This work investigates how QSOX and protein disulfide isomerase (PDI) cooperate in vitro to generate native pairings in two unfolded reduced...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801604x
更新日期:2008-11-18 00:00:00
abstract::The structural and functional aspects along with equilibrium unfolding of procerain, a cysteine protease from Calotropis procera, were studied in solution. The energetic parameters and conformational stability of procerain in different states were also estimated and interpreted. Procerain belongs to the alpha + beta c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035047m
更新日期:2003-10-28 00:00:00
abstract::Azide binds to fast cytochrome bo with a stoichiometry of 1:1, the dissociation constant for this reaction being approximately 2 x 10(-5) M. The changes induced in the electronic absorption are very slight and are consistent with heme o remaining hexacoordinate high-spin, an observation confirmed by room temperature M...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961221d
更新日期:1996-10-29 00:00:00
abstract::The Haemophilus influenzae diaminopimelate epimerase was cloned, expressed, purified, and crystallized in the C2221 space group (a = 102.1 A, b = 115.4 A, c = 66.3 A, alpha = beta = gamma = 90 degrees). The three-dimensional structure was solved to 2.7 A using a single Pt derivative and the Se-Met-substituted enzyme t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi982138o
更新日期:1998-11-24 00:00:00
abstract::Photoactive yellow proteins (PYPs) make up a diverse class of blue-light-absorbing bacterial photoreceptors. Electronic excitation of the p-coumaric acid chromophore covalently bound within PYP results in triphasic quenching kinetics; however, the molecular basis of this behavior remains unresolved. Here we explore th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b01114
更新日期:2018-03-20 00:00:00
abstract::The functional role of distinct phospholipid subclasses and molecular species in modulating gramicidin-mediated K+ flux was characterized through quantification of changes in the fluorescence intensity of ion specific fluorescent probes encapsulated inside vesicles comprised of individual molecular species of plasmeny...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00022a008
更新日期:1995-06-06 00:00:00
abstract::The kinetic and thermodynamic features of reactions catalyzed by present-day enzymes appear to be the consequence of the evolution of these proteins toward maximal catalytic effectiveness. These features are identified and analyzed (in detail for one substrate-one product enzymes) by using ideas that link the energeti...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00450a009
更新日期:1989-11-28 00:00:00
abstract::DNA photolyase from Escherichia coli contains FAD plus a partially characterized, second chromophore. In vivo, the flavin is fully reduced (FADH2), but oxidation to a stable, blue radical (FADH.) occurs during enzyme isolation. The second chromophore is irreversibly reduced by reaction of the enzyme with sodium borohy...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00395a034
更新日期:1987-10-20 00:00:00
abstract::Heme A, an obligatory cofactor in eukaryotic cytochrome c oxidase, is produced from heme B (protoheme) via two enzymatic reactions catalyzed by heme O synthase and heme A synthase. Heme O synthase is responsible for the addition of a farnesyl moiety, while heme A synthase catalyzes the oxidation of a methyl substituen...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0203536
更新日期:2002-09-10 00:00:00
abstract::Protein phosphatase-1 (PP1) is an essential protein Ser/Thr phosphatase that is extraordinarily conserved from yeast to human, and Inhibitor-2 (I-2) is the most ancient of the heat-stable proteins specific for PP1. We identified novel I-2 homologues in Caenorhabditis elegans (Ce) and Xenopus laevis (Xe) and compared t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi602369m
更新日期:2007-03-06 00:00:00
abstract::To better understand the mechanism of nitric oxide (NO) synthesis, we studied conversion of N-hydroxy-L-arginine (NOHA) or L-arginine (Arg) to citrulline and NO under single-turnover conditions using the oxygenase domain of neuronal nitric oxide synthase (nNOSoxy) and rapid scanning stopped-flow spectroscopy. When ana...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9920228
更新日期:2000-03-07 00:00:00
abstract::The cytological architecture of the synaptonemal complex (SC), a meiosis-specific proteinaceous structure, is evolutionarily conserved among eukaryotes. However, little is known about the biochemical properties of SC components or the mechanisms underlying their roles in meiotic chromosome synapsis and recombination. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi4005528
更新日期:2013-08-06 00:00:00
abstract::Extended illumination slows the rebinding of CO to myoglobin after photodissociation at cryogenic temperatures. Two types of models have been put forward to explain the effect: motions of the CO within the heme pocket or conformational transitions of the protein. To resolve this ambiguity, we have studied the effect o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00249a030
更新日期:1994-11-15 00:00:00
abstract::In investigating the agonist binding site of the human brain cholecystokininB receptor (CCKBR), we employed the direct protein chemical approach using a photoreactive tritiated analogue of sulfated cholecystokinin octapeptide, which contains the p-benzoylbenzoyl moiety at the N-terminus, followed by purification of th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990269z
更新日期:1999-05-11 00:00:00
abstract::Ca2+-free crystals of sarcoplasmic reticulum Ca2+-ATPase have, up until now, been obtained in the presence of inhibitors such as thapsigargin (TG), bound to the transmembrane region of this protein. Here, we examined the consequences of such binding for the protein. We found that, after TG binding, an active site liga...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701855r
更新日期:2007-12-25 00:00:00
abstract::Thioacetamide, a hepatocarcinogen and an inhibitor of heme synthesis, blocks the phenobarbitone-mediated increase in the transcription of cytochrome P-450b+e messenger RNA in rat liver. This property is also shared by CoCl2 and 3-amino-1,2,4-triazole, two other inhibitors of heme synthesis. Thus, it appears feasible t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00364a009
更新日期:1986-08-12 00:00:00
abstract::The ErbB family of tyrosine kinase receptors is a key element in preserving cell growth homeostasis. This family is comprised of four single-transmembrane domain proteins designated ErbB-1-4. Ligand binding initiates dimerization followed by tyrosine phosphorylation and signaling, which when uncontrolled lead to cance...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00450
更新日期:2016-10-04 00:00:00
abstract::Galectin-3 is a member of a large family of beta-galactoside-binding animal lectins and is composed of a carboxyl-terminal lectin domain connected to an amino-terminal nonlectin part. Previous experimental results suggest that, when bound to multivalent glycoconjugates, galectin-3 self-associates through intermolecula...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971409c
更新日期:1998-03-24 00:00:00
abstract::We have investigated the action of the human DNA polymerase ε (hpol ε) and η (hpol η) catalytic cores on G-quadruplex (G4) DNA substrates derived from the promoter of the c-MYC proto-oncogene. The translesion enzyme hpol η exhibits a 6.2-fold preference for binding to G4 DNA over non-G4 DNA, while hpol ε binds both G4...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00060
更新日期:2015-05-26 00:00:00
abstract::N-Ethylmaleimide modified heavy meromyosin in only 3-fold activated by actin rather than 200-fold as is normal heavy meromyosin (Silverman, R., Eisenberg, E., and Kielley, W. W. (1972), Nature (London) 240, 207). Ultracentrifuge studies demonstrated that in the absence of ATP the N-ethylmaleimide modified heavy merom...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00688a020
更新日期:1975-08-26 00:00:00
abstract::The Na+-translocating NADH:ubiquinone oxidoreductase (Na+-NQR) from Vibrio harveyi was purified and studied by EPR and visible spectroscopy. Two EPR signals in the NADH-reduced enzyme were detected: one, a radical signal, and the other a line around g = 1.94, which is typical for a [2Fe-2S] cluster. An E(m) of -267 mV...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002545b
更新日期:2001-06-19 00:00:00
abstract::Syk, a nonreceptor protein-tyrosine kinase, is activated by both oxidative and osmotic stress and plays different roles in the transduction of stress signals. In this study, the regulation of oxidative and osmotic stress induced Syk activation was investigated utilizing Syk-negative DT40 cells, expressing various Syk ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9729460
更新日期:1998-04-21 00:00:00
abstract::There is increasing evidence that a class of cell membrane glycolipids, gangliosides, can mediate the fibrillogenesis and toxicity of Alzheimer's disease amyloid-beta peptide (Abeta). Using lipid monolayers and vesicles as model membranes, we measured the insertion of Abeta into 1,2-dipalmitoyl-sn-glycero-3-phosphocho...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi062177x
更新日期:2007-02-20 00:00:00
abstract::Two forms of cytochrome P-450, designated P-450NMa and P-450NMb, were purified to electrophoretic homogeneity from rabbit nasal microsomes. The purified cytochromes, which contained 14-16 nmol of P-450/mg of protein, exhibited apparent monomeric molecular weights of 49,500 and 51,000, respectively. As indicated by sev...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00422a007
更新日期:1988-11-01 00:00:00
abstract::The electrostatic binding strength of water-soluble proteins having either an excess positive (cytochrome c) or negative (beta-lactoglobulin) electric charge to oppositely charged supported planar bilayers (SPBs) was studied as a function of the bilayer phase state (fluid or gel phase) by IR-ATR spectroscopy. The bila...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi991564m
更新日期:1999-11-16 00:00:00
abstract::Parathyroid hormone (PTH) regulates calcium and phosphate metabolism through a G-protein-coupled receptor which is shared with PTH-related protein (PTHrP). Therefore, structure-activity studies of PTH and PTHrP with their common receptor provide an unusual opportunity to examine the structural elements in the two horm...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00033a029
更新日期:1995-08-22 00:00:00
abstract::The qualitative and quantitative features of mutagenesis by two DNA adducts of carcinogenic alkylating agents, O6-methylguanine (m6G) and O4-methylthymine (m4T), were examined in vivo. The deoxyhexanucleotides 5'-GCTAGC-3' and 5'-GCTAGC-3' were synthesized, where the underlined bases are the positions of m4T or m6G, r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00242a031
更新日期:1991-07-16 00:00:00
abstract::BamA is an essential component of the β-barrel assembly machine (BAM) that is responsible for insertion and folding of β-barrel outer membrane proteins (OMPs) in Gram-negative bacteria. BamA is an OMP itself, and its β-barrel transmembrane domain is thought to catalyze OMP insertion and folding, although the molecular...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00281
更新日期:2017-06-20 00:00:00
abstract::Immunological methods, in parellel with measurement of ATPase activity, have been used to characterize the reactions of antibodies specific for light chains with myosin and its water-soluble proteolytic subfragments, heavy meromyosin (HMM) and subfragment 1 (HMM S-1). Antiserum to the 5,5'-dithiobis(2-nitro-enzoic aci...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00692a008
更新日期:1975-10-21 00:00:00
abstract::The Pseudomonas aeruginosa FpvA receptor is a TonB-dependent outer membrane transport protein that catalyzes uptake of ferric pyoverdin across the outer membrane. Surprisingly, FpvA expressed in P. aeruginosa grown in an iron-deficient medium copurifies with a ligand X that we have characterized by UV, fluorescence, a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990421x
更新日期:1999-07-20 00:00:00