Abstract：
:Azide binds to fast cytochrome bo with a stoichiometry of 1:1, the dissociation constant for this reaction being approximately 2 x 10(-5) M. The changes induced in the electronic absorption are very slight and are consistent with heme o remaining hexacoordinate high-spin, an observation confirmed by room temperature MCD spectroscopy in the region 350-2000 nm. X-band EPR spectroscopy of the azide-bound form shows heme o remains coupled to CuB, but that the integer spin signal (g = 3.7) that we have previously reported to be associated with the binuclear center of fast cytochrome bo [Watmough et al. (1993) FEBS Lett. 319, 151-154], is shifted to higher field. The kinetics of azide binding are an order of magnitude faster than those observed for the binding of cyanide. Unlike cyanide, the observed rate constants do not saturate in the range 0.05-25 mM. The value of Kon shows a marked dependence on pH, indicating that the active species is hydrazoic acid. It is argued that these data are consistent with the binding of azide ion as a terminal ligand to CuB yielding a binuclear center in the form FeIII-OH2:: CuBII-N3. The binding of azide in heme-copper oxidases may cause displacement of another nitrogenous ligand from CuB which might explain the absence of electron density associated with histidine-325 in the structure of the Paracoccus denitrificans CCO [Iwata et al. (1995) Nature 376, 660-669]. Formate appears to act as a bidentate ligand to the binuclear center-, blocking not only the binding of azide to CuB but also the binding of cyanide to heme o.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Little RH,Cheesman MR,Thomson AJ,Greenwood C,Watmough NJdoi
10.1021/bi961221dsubject
Has Abstractpub_date
1996-10-29 00:00:00pages
13780-7issue
43eissn
0006-2960issn
1520-4995pii
bi961221djournal_volume
35pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Two terminally blocked peptides, acetylAETAAAKFLRQHMamide and acetylAETSSSRYLRQHMamide, were obtained by solid-phase synthesis, purified by reversed-phase chromatography, and characterized by fast atom bombardment mass spectrometry. Both peptides were soluble in aqueous solutions and remained monomeric over the concen...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00427a048
更新日期：1989-01-10 00:00:00
abstract：:The RcnR metalloregulator represses the transcription of the Co(II) and Ni(II) exporter, RcnAB. Previous studies have shown that Co(II) and Ni(II) bind to RcnR in six-coordinate sites, resulting in derepression. Here, the roles of His60, His64, and His67 in specific metal recognition are examined. His60 and His64 corr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300886q
更新日期：2013-01-08 00:00:00
abstract：:The interaction of the recombinant hemoglobin from Vitreoscilla sp. (VHb) with the bacterial membrane of Escherichia coli cells has been investigated by measuring the propensity of VHb to interact with monolayers formed by natural bacterial phosholipids. The measurements showed that the protein is capable of penetrati...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi052277n
更新日期：2006-04-04 00:00:00
abstract：:The ATF (activating transcription factor) binding site within the E2A promoter region of adenovirus is shown to exist in a novel conformation in vitro via nuclear magnetic resonance methods. This novel conformation may be important to the protein DNA recognition process. This conformation has characteristics of both A...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00077a003
更新日期：1993-07-06 00:00:00
abstract：:Budding of retroviruses requires the structural precursor polyprotein, Gag, to target the plasma membrane through its N-terminal matrix (MA) domain. For HIV-1, the interaction between membrane signaling molecule phosphatidylinositol 4,5-diphosphate (PIP2) and MA induces the exposure of myristate and promotes membrane ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701984h
更新日期：2008-02-19 00:00:00
abstract：:Some aminoacyl-tRNA synthetases (AARSs) employ an editing mechanism to ensure the fidelity of protein synthesis. Leucyl-tRNA synthetase (LeuRS), isoleucyl-tRNA synthetase (IleRS), and valyl-tRNA synthetase (ValRS) share a common insertion, called the CP1 domain, which is responsible for clearing misformed products. Th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi702494q
更新日期：2008-04-22 00:00:00
abstract：:A new method is described for determining molecular structures from NMR data. The approach utilizes 2D NOESY back-calculations to generate simulated spectra for structures obtained from distance geometry (DG) computations. Comparison of experimental and back-calculated spectra, including analysis of cross-peak buildup...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00454a005
更新日期：1990-01-16 00:00:00
abstract：:The temperature dependence of the reduction potential of the CuA site in carbon monoxide inhibited cytochrome c oxidase has been measured with a spectroelectrochemical method adapted to the relatively weak near-infrared absorption of this copper ion. These measurements, together with parallel measurements on the 604-n...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00349a024
更新日期：1986-01-14 00:00:00
abstract：:We have measured the frequency of the carbon-hydrogen stretching mode of the pro-R and pro-S C4-H bonds of NADH in solution and when bound to pig heart lactate (LDH) or mitochondrial malate (mMDH) dehydrogenases. This is achieved by specifically deuterating the C4 pro-R or pro-S hydrogens of NADH and determining the f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00136a022
更新日期：1992-06-02 00:00:00
abstract：:Two-dimensional nuclear magnetic resonance techniques were used to assign the NH, C alpha H, and C beta H protons of over 60 of the 104 amino acid residues in the 1H NMR spectrum of horse ferrocytochrome c. The majority of these amino acids were completely assigned. Assignments were based on the analysis of two-dimens...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00341a002
更新日期：1985-09-24 00:00:00
abstract：:We synthesized the following four new peptide substrates, Suc-Phe-Leu-pNA, Suc-Phe-Leu-NMec, Suc-Phe-Leu-ONPh, and Pht-Phe-Leu-pNA, and we applied the proton inventory method to their hydrolysis by papain. Useful relationships between the rate constants of the catalytic reaction have been established and contributed t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi001615b
更新日期：2001-04-03 00:00:00
abstract：:A modified form of procollagen was extracted with 10 M urea from the skin of lambs with dermatosparaxis, a disease which is produced by a genetic defect in the conversion of procollagen to collagen. The extracts contained little if any alpha1 and alpha2 chains of normal type I collagen, and instead they contained the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00658a024
更新日期：1976-06-29 00:00:00
abstract：:A five-residue loop structure in basic fibroblast growth factor (FGF-2) which extends from amino acid residue 118 to residue 122 was replaced, by cassette mutagenesis, with the corresponding seven-residue loop structure from the structural homologue acidic fibroblast growth factor (FGF-1) or the corresponding five-res...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00003a004
更新日期：1995-01-24 00:00:00
abstract：:The respiratory chain of Vibrio cholerae contains three bd-type quinol oxygen reductases as well as one cbb(3) oxygen reductase. The cbb(3) oxygen reductase has been previously isolated and characterized; however, the natural mobile electron donor(s) that shuttles electrons between the bc(1) complex and the cbb(3) oxy...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi1004574
更新日期：2010-09-07 00:00:00
abstract：:The processes of liposome solubilization and reconstitution were studied by using n-dodecyl octaethylene glycol monoether (C12E8). The solubilization of large unilamellar liposomes prepared by reverse-phase evaporation was systematically investigated by turbidity, 31P nuclear magnetic resonance, and centrifugation exp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00492a022
更新日期：1990-10-09 00:00:00
abstract：:RP2 is a ubiquitously expressed protein encoded by a gene associated with X-linked retinitis pigmentosa (XLRP), a retinal degenerative disease that causes severe vision loss. Previous in vitro studies have shown that RP2 binds to ADP ribosylation factor-like 3 (Arl3) and activates its intrinsic GTPase activity, but th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi1005249
更新日期：2010-09-07 00:00:00
abstract：:The interaction of alamethicin with artificial lecithin multilamellar dispersions was investigated by nuclear magnetic resonance (NMR) and Raman spectroscopies. 31P NMR studies revealed perturbation of the lipid head groups in the presence of the icosapeptide. Simulation of the 31P NMR spectra indicated that the obser...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00347a019
更新日期：1985-12-17 00:00:00
abstract：:A soluble and fully functional 10.5 kDa fragment of the 18.2 kDa membrane-bound cytochrome c(552) from Paracoccus denitrificans has been heterologously expressed and (13)C/(15)N-labeled to study the structural features of this protein in both redox states. Well-resolved solution structures of both the reduced and oxid...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi010615o
更新日期：2001-10-16 00:00:00
abstract：:The exopeptidases dipeptidyl aminopeptidases I and IV were used to hydrolyze the N-terminal portion of spinach plastocyanin to dipeptides. The enzymes were used individually as well as in a mixture and the dipeptides were analyzed by combined gas chromatography-mass spectrometry. Data are presented for native plastocy...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00596a009
更新日期：1978-02-07 00:00:00
abstract：:Gluconacetobacter diazotrophicus stands out among the acetic acid bacteria as it fixes dinitrogen and is a true endophyte. It has a set of constitutive enzymes to oxidize ethanol and acetaldehyde which is upregulated during N(2)-dependent growth. The membrane-bound alcohol dehydrogenase (ADH) is a heterodimer (subunit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9015007
更新日期：2010-03-23 00:00:00
abstract：:The kinetics of charge recombination following photoexcitation by a laser pulse have been analyzed in the reaction center-light harvesting complex 1 (RC-LH1) purified from the photosynthetic bacterium Rhodobacter sphaeroides. In RC-LH1 core complexes isolated from photosynthetically grown cells P(+)Q(B)(-) recombines ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048629s
更新日期：2004-11-09 00:00:00
abstract：:The members of the ets gene family of transcription factors are characterized by a conserved 85-residue DNA-binding region, termed the ETS domain, that lacks sequence homology to structurally characterized DNA-binding motifs. The secondary structure of the ETS domain of murine Ets-1 was determined on the basis of NMR ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00250a001
更新日期：1994-11-22 00:00:00
abstract：:Thymidylate synthase from phage T4 (T4TS) is part of a complex of several enzymes required for coordinate DNA synthesis in infected Escherichia coli cells. It has been proposed that similar complexes of enzymes related to DNA synthesis are also functional in eukaryotes [Pardee, A. B. (1989) Science 246, 603-608]. To d...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00255a028
更新日期：1994-12-27 00:00:00
abstract：:α-Synuclein (α-Syn) oligomerization and amyloid formation are associated with Parkinson's disease (PD) pathogenesis. Studying familial α-Syn mutants associated with early onset PD has therapeutic importance. Here we report the aggregation kinetics and other biophysical properties of a newly discovered PD associated Fi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi5010365
更新日期：2014-10-21 00:00:00
abstract：:We have synthesized Deltalac-acetogenins that are new acetogenin mimics possessing two n-alkyl tails without an alpha,beta-unsaturated gamma-lactone ring and suggested that their inhibition mechanism may be different from that of common acetogenins [Hamada et al. (2004) Biochemistry 43, 3651-3658]. To elucidate the in...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0479003
更新日期：2005-01-18 00:00:00
abstract：:Phage T4 gene 32 protein (gp32) is a zinc metalloprotein which binds cooperatively and preferentially to single-stranded nucleic acids and functions as a replication and recombination accessory protein. Zn(II) coordination by gp32 employs a His-Cys3 metal ligand donor set derived from the His64-X12-Cys77-X9-Cys87-X2-C...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9617769
更新日期：1997-01-28 00:00:00
abstract：:Design of a partially cysteine-depleted C98S/C239S/C377S/C468A cytochrome P450 3A4 mutant designated CYP3A4(C58,C64) allowed site-directed incorporation of thiol-reactive fluorescent probes into alpha-helix A. The site of modification was identified as Cys-64 with the help of CYP3A4(C58) and CYP3A4(C64), each bearing ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061944p
更新日期：2007-01-09 00:00:00
abstract：:A ribonuclease that hydrolyzes either linear duplex or single-stranded RNA in an exonucleolytic manner has been partially purified from Ehrlich ascites tumor cell nucleoli and is free from other ribonucleases. The enzyme will also degrade the RNA complement of an RNA X DNA duplex; however, no nuclease activity is obse...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00324a022
更新日期：1985-01-29 00:00:00
abstract：:Energy coupling factor (ECF) transporters take up micronutrients in Bacteria and Archaea. They consist of a membrane-embedded S-component that provides substrate specificity and a three-subunit ECF module that couples ATP hydrolysis to transport. The S-components ThiT (for thiamin) and NiaX (for niacin) from Lactococc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00609
更新日期：2015-08-11 00:00:00
abstract：:The cooperative binding of the Escherichia coli DNA unwinding protein to single-stranded DNA has been studied by electron microscopy. Analysis of the electron microscopic data by means of a simple statistical mechanical model has yielded a value of 3.8-7.6 X 10(10) l./mol for the cooperative binding constant in 0.15 M...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00696a023
更新日期：1975-12-16 00:00:00
