Abstract:
:The interaction of alamethicin with artificial lecithin multilamellar dispersions was investigated by nuclear magnetic resonance (NMR) and Raman spectroscopies. 31P NMR studies revealed perturbation of the lipid head groups in the presence of the icosapeptide. Simulation of the 31P NMR spectra indicated that the observed spectral changes could be attributed to slight variations in the average tilt angle of the head groups. In contrast, no noticeable effect of the peptide on the segmental order of the hydrophobic acyl chains of the lipid molecules was detected by 2H NMR and Raman spectroscopic measurements. Taken together, these results indicated that, in the absence of a transmembrane electric potential, alamethicin interacts primarily at the water-lipid interface without significant insertion or incorporation into the bilayer leaflet.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Banerjee U,Zidovetzki R,Birge RR,Chan SIdoi
10.1021/bi00347a019subject
Has Abstractpub_date
1985-12-17 00:00:00pages
7621-7issue
26eissn
0006-2960issn
1520-4995journal_volume
24pub_type
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