Proton transfer in benzyl alcohol dehydrogenase during catalysis: alternate proton-relay routes.

abstract：:The GTP-binding proteins or GTPases comprise a superfamily of proteins that provide molecular switches in numerous cellular processes. The "GTPase switch" paradigm, in which a GTPase acts as a bimodal switch that is turned "on" and "off" by external regulatory factors, has been used to interpret the regulatory mechani...

journal_title：Biochemistry

authors： Shan SO,Schmid SL,Zhang X

更新日期：2009-07-28 00:00:00

abstract：:Four mechanisms for the allosteric regulation of the calcium and magnesium ion activated adenosinetriphosphatase (Ca,Mg-ATPase) of sarcoplasmic reticulum were examined. Negative cooperativity in substrate binding was not supported by 3H-labeled 5'-adenylyl methylenediphosphate (AMPPCP) binding, which was best fit by a...

journal_title：Biochemistry

authors： Cable MB,Feher JJ,Briggs FN

更新日期：1985-09-24 00:00:00

abstract：:Paracoccus denitrificans synthesizes an inducible, periplasmic, blue copper protein [Husain, M., & Davidson, V.L. (1985) J. Biol. Chem. 260, 14626-14629] that can be classified as an amicyanin on the basis of its ability to accept electrons from methylamine dehydrogenase. The amino acid composition and sequence of the...

journal_title：Biochemistry

authors： Husain M,Davidson VL,Smith AJ

更新日期：1986-05-06 00:00:00

abstract：:The human intestinal Caco-2 cell line, described as enterocyte-like in a number of studies, was examined for its ability to carry out the metabolism of vitamin A normally required in the absorptive process. Caco-2 cells contained cellular retinol-binding protein II, a protein which is abundant in human villus-associat...

journal_title：Biochemistry

authors： Quick TC,Ong DE

更新日期：1990-12-18 00:00:00

abstract：:Histidinol-phosphate aminotransferase (HspAT) is a key enzyme on the histidine biosynthetic pathway. HspAT catalyzes the transfer of the amino group of L-histidinol phosphate (Hsp) to 2-oxoglutarate to form imidazole acetol phosphate (IAP) and glutamate. Thus, HspAT recognizes two kinds of substrates, Hsp and glutamat...

journal_title：Biochemistry

authors： Haruyama K,Nakai T,Miyahara I,Hirotsu K,Mizuguchi H,Hayashi H,Kagamiyama H

更新日期：2001-04-17 00:00:00

abstract：:The gene for human pancreatic cholesterol esterase consists of 11 exons and 10 introns and is 9.2 kb in length. The last and longest exon (841 nucleotides) is unique to the human gene. Functional amino acids are encoded on separate exons. The leader sequence is encoded by a single exon which carries two additional N-t...

journal_title：Biochemistry

authors： Kumar BV,Aleman-Gomez JA,Colwell N,Lopez-Candales A,Bosner MS,Spilburg CA,Lowe M,Lange LG

更新日期：1992-07-07 00:00:00

abstract：:Polyhistidine affinity tags are routinely employed as a convenient means of purifying recombinantly expressed proteins. A tacit assumption is commonly made that His tags have little influence on protein structure and function. Attachment of a His tag to the N-terminus of the robust globular protein myoglobin leads to ...

journal_title：Biochemistry

authors： Thielges MC,Chung JK,Axup JY,Fayer MD

更新日期：2011-06-28 00:00:00

abstract：:Most terpene synthase reactions follow Markovnikov rules for formation of high-energy carbenium ion intermediates. However, there are notable exceptions. For example, pentalenene synthase (PS) undergoes an initial anti-Markovnikov cyclization reaction followed by a 1,2-hydride shift to form an intermediate humulyl cat...

journal_title：Biochemistry

authors： Matos JO,Kumar RP,Ma AC,Patterson M,Krauss IJ,Oprian DD

更新日期：2020-09-08 00:00:00

abstract：:The transfer of ganglioside GM1 from micelles to membranes and between different membrane populations has been examined by using a pyrene fatty acid derivative of the ganglioside. The transfer of gangliosides from micelles to membranes depends on the physical state as well as the molecular composition of the acceptor ...

journal_title：Biochemistry

authors： Masserini M,Freire E

更新日期：1987-01-13 00:00:00

abstract：:A bifunctional molecule was genetically engineered which contained an amino-terminal effector domain that bound immunoglobulin Fc (fragment B of staphylococcal protein A) and a carboxyl-terminal domain that bound digoxin [a single-chain Fv (sFv)]. Effector and sFv binding properties were virtually identical with those...

journal_title：Biochemistry

authors： Tai MS,Mudgett-Hunter M,Levinson D,Wu GM,Haber E,Oppermann H,Huston JS

更新日期：1990-09-04 00:00:00

abstract：:Rat mucosal-type mast cells of the RBL-2H3 line express a glycoprotein termed the MAst cell Function-associated Antigen (MAFA). When MAFA is clustered by its specific monoclonal antibody G63, secretion normally triggered by aggregating these cells' type I Fcepsilon receptor (FcepsilonRI) is substantially inhibited. Th...

journal_title：Biochemistry

authors： Song J,Hagen GM,Roess DA,Pecht I,Barisas BG

更新日期：2002-01-22 00:00:00

abstract：:A small metal-binding protein (SmbP) with no known similarity to other proteins in current databases was isolated and characterized from the periplasm of Nitrosomonas europaea. The primary structure of this small (9.9 kDa) monomeric protein is characterized by a series of 10 repeats of a seven amino acid motif and an ...

journal_title：Biochemistry

authors： Barney BM,LoBrutto R,Francisco WA

更新日期：2004-09-07 00:00:00

abstract：:Calcium trapped within sonicated and resealed bovine rod outer segment disks is released upon light exposure with a stoichiometry of 0.75 +/- 0.05 calcium for each rhodopsin bleached. The amount of calcium liberated is proportional to the amount of bleaching in the range of 20 to 100% bleaching and is relatively insen...

journal_title：Biochemistry

authors： Smith HG Jr,Fager RS,Litman RJ

更新日期：1977-04-05 00:00:00

abstract：:The subtilisins have an extended substrate binding cleft comprising at least 8 subsites. Two pockets at the S1 and S4 sites are particularly conspicuous, and the interactions between substrate and these two pockets are very important for the substrate specificity. Phe residues have mutationally been introduced at one ...

journal_title：Biochemistry

authors： Bech LM,Sørensen SB,Breddam K

更新日期：1993-03-23 00:00:00

abstract：:Carboxyl ester lipase (CEL; EC 3.1.1.13) hydrolyzes cholesteryl esters and retinyl esters in vitro. In vivo, pancreatic CEL is thought to liberate cholesterol and retinol from their esters prior to absorption in the intestine. CEL is also a major lipase in the breast milk of many mammals, including humans and mice, an...

journal_title：Biochemistry

authors： Weng W,Li L,van Bennekum AM,Potter SH,Harrison EH,Blaner WS,Breslow JL,Fisher EA

更新日期：1999-03-30 00:00:00

abstract：:The folding kinetics of a 16-residue beta-hairpin (trpzip4) and five mutants were studied by a laser-induced temperature-jump infrared method. Our results indicate that mutations which affect the strength of the hydrophobic cluster lead to a decrease in the thermal stability of the beta-hairpin, as a result of increas...

journal_title：Biochemistry

authors： Du D,Tucker MJ,Gai F

更新日期：2006-02-28 00:00:00

abstract：:A number of nucleosides related to S-adenosylmethionine were tested for their inhibitory action on three enzymes involved in the biosynthesis of polyamines. The particular objective of the experiments was to determine whether any of the compounds could be used as selective inhibitors of the synthesis of spermine by sp...

journal_title：Biochemistry

authors： Pegg AE,Coward JK,Talekar RR,Secrist JA 3rd

更新日期：1986-07-15 00:00:00

abstract：:Type IIS restriction endonuclease Eco31I harbors a single HNH active site and cleaves both DNA strands close to its recognition sequence, 5'-GGTCTC(1/5). A two-domain organization of Eco31I was determined by limited proteolysis. Analysis of proteolytic fragments revealed that the N-terminal domain of Eco31I is respons...

journal_title：Biochemistry

authors： Jakubauskas A,Sasnauskas G,Giedriene J,Janulaitis A

更新日期：2008-08-19 00:00:00

abstract：:Polyadenylated RNA was isolated from nuclei of cultured Drosophila cells, Schneider's line 2, and used as a template to synthesize a complementary DNA probe. Hybridization experiments were performed to study the relationship between nuclear and cytoplasmic RNA. About two-thirds of the nuclear polyadenylated RNA sequen...

journal_title：Biochemistry

authors： Levy B,McCarthy BJ

更新日期：1976-06-01 00:00:00

abstract：:The crystal structure and spectroscopic properties of the periplasmic penta-heme cytochrome c nitrite reductase (NrfA) of Escherichia coli are presented. The structure is the first for a member of the NrfA subgroup that utilize a soluble penta-heme cytochrome, NrfB, as a redox partner. Comparison to the structures of ...

journal_title：Biochemistry

authors： Bamford VA,Angove HC,Seward HE,Thomson AJ,Cole JA,Butt JN,Hemmings AM,Richardson DJ

更新日期：2002-03-05 00:00:00

abstract：:Apurinic/apyrimidinic endonuclease 1 (APE1) is an Mg²⁺-dependent enzyme responsible for incising the DNA backbone 5' to an apurinic/apyrimidinic (AP) site. Here, we use rapid quench flow (RQF) techniques to provide a comprehensive kinetic analysis of the strand-incision activity (k(chemistry)) of APE1 acting on an aut...

journal_title：Biochemistry

authors： Schermerhorn KM,Delaney S

更新日期：2013-10-29 00:00:00

abstract：:Nucleotide excision repair (NER) is a crucial pathway in the maintenance of genome stability requiring at least two dozen proteins. XPA and RPA have essential roles in the damage recognition step of NER. To better understand the mechanism of their interactions with DNA, we utilized equilibrium and stop-flow kinetic ap...

journal_title：Biochemistry

authors： Iakoucheva LM,Walker RK,van Houten B,Ackerman EJ

更新日期：2002-01-08 00:00:00

abstract：:Members of the PP1 family of protein phosphatases achieve functional diversity through numerous and varied protein-protein interactions. In mammals, there are four PP1 isoforms, the ubiquitously expressed PPP1CA, PPP1CB, and PPP1CC1, and the testis specific splice isoform PPP1CC2. When the mouse Ppp1cc gene is deleted...

journal_title：Biochemistry

authors： MacLeod G,Varmuza S

更新日期：2012-12-04 00:00:00

abstract：:The causes of the salting-in of beta-lactoglobulin by glycine and NaCl, a solubility behavior contrary to expectations, were probed by a detailed study of the interactions between these solvent components and the protein. The preferential interactions of beta-lactoglobulin with solvent components in aqueous glycine an...

journal_title：Biochemistry

authors： Arakawa T,Timasheff SN

更新日期：1987-08-11 00:00:00

abstract：:Picosecond transient absorption difference spectroscopic experiments were performed on membranes of the antenna/reaction center complex of Heliobacillus mobilis to study the electron transfer processes. Particular emphasis was placed on the blue spectral region, where the difference spectra of iron-sulfur centers and ...

journal_title：Biochemistry

authors： Lin S,Chiou HC,Blankenship RE

更新日期：1995-10-03 00:00:00

abstract：:Transcarboxylase (TC) from Propionibacterium shermanii, a biotin-dependent enzyme, catalyzes the transfer of a carboxyl group from methylmalonyl-CoA to pyruvate to form propionyl-CoA and oxalacetate. Within the multi-subunit enzyme complex, the 1.3S subunit functions as the carboxyl group carrier and also binds the ot...

journal_title：Biochemistry

authors： Reddy DV,Shenoy BC,Carey PR,Sönnichsen FD

更新日期：2000-03-14 00:00:00

abstract：:It has been shown (Poole et al., 1991) that deletion of residues 44-49 from the sequence of staphylococcal nuclease (E43 SNase) results in an enzyme (E43 delta SNase) that is significantly more active than D43 SNase, an enzyme that differs from the wild-type enzyme by deletion of a single methylene group. In addition,...

journal_title：Biochemistry

authors： Baldisseri DM,Torchia DA,Poole LB,Gerlt JA

更新日期：1991-04-16 00:00:00

abstract：:Type II topoisomerases are responsible for DNA unlinking during DNA replication and chromosome segregation. Although eukaryotic enzymes are homodimers and prokaryotic enzymes are heterotetramers, both prokaryotic and eukaryotic type II topoisomerases belong to a single protein family. The amino- and carboxyl-terminal ...

journal_title：Biochemistry

authors： Lavasani LS,Hiasa H

更新日期：2001-07-24 00:00:00

abstract：:DNA sequences containing homopurine d(G1-3A)n tracts are known to be capable of adopting non-B-DNA conformations. The structural polymorphism of these sequences is a direct consequence of the structural properties of the homopurine d(G1-3A)n tracts. Depending on the conditions, d(GA)n DNA sequences can form antiparall...

journal_title：Biochemistry

authors： Huertas D,Azorín F

更新日期：1996-10-08 00:00:00

abstract：:Biological membranes exhibit an asymmetric distribution of phospholipids. Phosphatidylserine (PS) is an acidic phospholipid that is found almost entirely on the interior of the cell where it is important for interaction with many cellular components. A less well understood phenomenon is the asymmetry of the neutral ph...

journal_title：Biochemistry

authors： Bazzi MD,Youakim MA,Nelsestuen GL

更新日期：1992-02-04 00:00:00