Tandem affinity purification in transgenic mouse embryonic stem cells identifies DDOST as a novel PPP1CC2 interacting protein.

abstract：:Calmodulin is a ubiquitous calcium dependent protein. In the presence of calcium, calmodulin adopts an altered conformation that leads to the generation of downstream cellular calcium signals. Here we describe the introduction of nitroxide EPR probes into calmodulin by means of site-directed spin labeling. These probe...

journal_title：Biochemistry

authors： Shao J,Cieslak J,Gross A

更新日期：2009-01-27 00:00:00

abstract：:Pancreatic phospholipase A(2) (PLA2) shows a strong preference for the binding to the anionic interface and a consequent allosteric activation. In this paper, we show that virtually all the preference is mediated through 3 (Lys-53, -56, and -120) of the 12 cationic residues of bovine pancreatic PLA2. The lysine-to-met...

journal_title：Biochemistry

authors： Yu BZ,Poi MJ,Ramagopal UA,Jain R,Ramakumar S,Berg OG,Tsai MD,Sekar K,Jain MK

更新日期：2000-10-10 00:00:00

abstract：:The reactions of metmyoglobin (metMb) and methemoglobin (metHb), oxidized to their respective oxoferryl free radical species (.Mb-FeIV = O/.Hb-4FeIV = O) by tert-butyl hydroperoxide (t-BuOOH), with nitric oxide (NO.) were studied by a combination of optical, electron spin resonance (ESR), ionspray mass (MS), fluoresce...

journal_title：Biochemistry

authors： Gorbunov NV,Osipov AN,Day BW,Zayas-Rivera B,Kagan VE,Elsayed NM

更新日期：1995-05-23 00:00:00

abstract：:Development of potent and isoform selective nitric oxide synthase (NOS) inhibitors is challenging because of the structural similarity in the heme active sites. One amino acid difference between NOS isoforms, Asp597 in rat neuronal NOS (nNOS) versus Asn368 in bovine endothelial NOS (eNOS), has been identified as the s...

journal_title：Biochemistry

authors： Li H,Wang HY,Kang S,Silverman RB,Poulos TL

更新日期：2016-07-05 00:00:00

abstract：:Protein S is an anticoagulant protein containing a Gla (enclosing gamma-carboxyglutamic acids) module, a TSR (thrombin sensitive region) module, four EGF (epidermal growth factor)-like modules, and a SHBG (sex hormone binding globulin)-like region. Protein S is a cofactor to activated protein C (APC) in the degradatio...

journal_title：Biochemistry

authors： Persson KE,Stenflo J,Linse S,Stenberg Y,Preston RJ,Lane DA,Rezende SM

更新日期：2006-09-05 00:00:00

abstract：:We have investigated the role of the labile terminal domains of the core histones on the stability of the subunits of the protein core of the nucleosome by studying the thermodynamic behavior of the products of limited trypsin digestion of these subunits. The thermal stabilities of the truncated H2A-H2B dimer and the ...

journal_title：Biochemistry

authors： Karantza V,Freire E,Moudrianakis EN

更新日期：2001-10-30 00:00:00

abstract：:Guanylin, an intestinal peptide hormone and endogenous ligand of guanylyl cyclase C, is produced as the corresponding prohormone proguanylin. The mature hormone consists of 15 amino acid residues, representing the COOH-terminal part of the prohormone comprised of 94 amino acid residues. Here we report the recombinant ...

journal_title：Biochemistry

authors： Lauber T,Nourse A,Schulz A,Marx UC

更新日期：2002-12-10 00:00:00

abstract：:The nitrogenase MoFe protein contains the active site metallocluster called FeMo-cofactor [7Fe-9S-Mo-homocitrate] that exhibits an S = 3/2 EPR signal in the resting state. No interaction with FeMo-cofactor is detected when either substrates or inhibitors are incubated with MoFe protein in the resting state. Rather, th...

journal_title：Biochemistry

authors： Benton PM,Mayer SM,Shao J,Hoffman BM,Dean DR,Seefeldt LC

更新日期：2001-11-20 00:00:00

abstract：:In the absence of any external electron donor, the "peroxy" intermediate of cytochrome c oxidase (CcO-607) is converted to the ferryl form (CcO-580) and subsequently to oxidized enzyme. The rate of conversion of CcO-607 to the CcO-580 form is pH dependent between pH 3.0 and pH 7.6. A plot of the logarithm of the rate ...

journal_title：Biochemistry

authors： Fabian M,Palmer G

更新日期：2001-02-13 00:00:00

abstract：:The natural ATPase inhibitor (IF1) from beef heart mitochondria was labeled with phenyl (14C)isothiocyanate [(14C)PITC]. Chemical labeling by (14C)PITC does not modify the inhibitory properties of IF1, provided the number of residues of (14C)PITC bound per molecule of IF1 is lower than five to six, which corresponds t...

journal_title：Biochemistry

authors： Klein G,Satre M,Dianoux AC,Vignais PV

更新日期：1980-06-24 00:00:00

abstract：:A PS I membrane preparation from a PS II deficient mutant of Synechocystis sp. PCC 6803 (psb DI/DII/C) was investigated by picosecond photovoltage and fluorescence measurements. The photovoltage kinetics show two distinct phases. At low excitation energies the fast phase correlates with the fluorescence decay time con...

journal_title：Biochemistry

authors： Hecks B,Wulf K,Breton J,Leibl W,Trissl HW

更新日期：1994-07-26 00:00:00

abstract：:Stopped-flow experiments obtained in the pre-steady-state time scale of the NAD-malic enzyme reaction exhibit a lag prior to the attainment of steady state. Previous results from isotope effect studies in which the deuterium isotope effect on Vmax decreases to a value of 1 at low pH have been interpreted as suggesting...

journal_title：Biochemistry

authors： Rajapaksa R,Abu-Soud H,Raushel FM,Harris BG,Cook PF

更新日期：1993-03-02 00:00:00

abstract：:Factor VIII (FVIII) is the nonproteolytic cofactor for FIXa in the tenase complex of blood coagulation. FVIII is proteolytically activated by thrombin and FXa in vitro to form a heterotrimer with full procoagulant activity. Activated protein C inactivates thrombin-activated FVIII through cleavage adjacent to position ...

journal_title：Biochemistry

authors： O'Brien DP,Johnson D,Byfield P,Tuddenham EG

更新日期：1992-03-17 00:00:00

abstract：:A series of double-helical oligodeoxyribonucleotides of sequence corresponding to a frame-shift mutational hot spot in the lambda CI gene, 5'-dGATGGGGCAG, are compared by proton magnetic resonance spectroscopy at 500 MHz of the exchangeable protons. Duplexes containing an extra guanine in a run of two, three, and four...

journal_title：Biochemistry

authors： Woodson SA,Crothers DM

更新日期：1988-01-12 00:00:00

abstract：:Three sites of N(G),N(G)-arginine methylation have been located at residues 205, 217, and 224 in the glycine-rich, COOH-terminal one-third of the HeLa A1 heterogeneous ribonucleoprotein. Together with the previously determined dimethylated arginine at position 193 [Williams et al., (1985) Proc. Natl. Acad. Sci. U.S.A....

journal_title：Biochemistry

authors： Kim S,Merrill BM,Rajpurohit R,Kumar A,Stone KL,Papov VV,Schneiders JM,Szer W,Wilson SH,Paik WK,Williams KR

更新日期：1997-04-29 00:00:00

abstract：:The backbone dynamics of uniformly 15N-labeled interleukin-1 beta are investigated by using two-dimensional inverse detected heteronuclear 15N-1H NMR spectroscopy. 15N T1, T2, and NOE data at a spectrometer frequency of 600 MHz are obtained for 90% of the backbone amide groups. The data provide evidence for motions on...

journal_title：Biochemistry

authors： Clore GM,Driscoll PC,Wingfield PT,Gronenborn AM

更新日期：1990-08-14 00:00:00

abstract：:Copper thiosemicarbazones cause considerable oxidative stress. This effect may be related to their cytotoxicity. In the present work, the chemical and cellular properties of a new ligand, pyridoxal thiosemicarbazone (H2T), and its copper(II) chelate (CuT) are assessed. CuT is toxic to cultured Ehrlich ascites tumor ce...

journal_title：Biochemistry

authors： Byrnes RW,Mohan M,Antholine WE,Xu RX,Petering DH

更新日期：1990-07-31 00:00:00

abstract：:S-Adenosylmethionine decarboxylase (AdoMetDC) is a key enzyme in the polyamine biosynthetic pathway. Inhibition of this pathway and subsequent depletion of polyamine levels is a viable strategy for cancer chemotherapy and for the treatment of parasitic diseases. Substrate analogue inhibitors display an absolute requir...

journal_title：Biochemistry

authors： Bale S,Brooks W,Hanes JW,Mahesan AM,Guida WC,Ealick SE

更新日期：2009-07-14 00:00:00

abstract：:Aspartate transcarbamoylase from Escherichia coli is a dodecameric enzyme consisting of two trimeric catalytic subunits and three dimeric regulatory subunits. The X-ray structure of this enzyme indicates that the side chains of His-41, Asp-100, and Asp-90 from one catalytic chain form interactions with the side chains...

journal_title：Biochemistry

authors： Baker DP,Kantrowitz ER

更新日期：1993-09-28 00:00:00

abstract：:A pathogenic bacterium has its own mechanisms for not only pathogenic attack but also exogenous invasion defense, in which the bacterial cell wall is the front line of attack and defense. We developed a biochemical lanthanide-encoding approach to quantify the uncanonical d-amino acid (d-X) that was edited in a small p...

journal_title：Biochemistry

authors： Zhao W,Liang Y,Yan X,Yang L,Wang Q

更新日期：2020-09-29 00:00:00

abstract：:Thermostable direct hemolysin (TDH) of Vibrio parahemolyticus is a membrane-damaging pore-forming toxin with potent cytolytic/cytotoxic activity. TDH exists as a tetramer consisting of protomers with a core β-sandwich domain, flanked by an 11-amino acid long N-terminal region (NTR). This NTR could not be modeled in th...

journal_title：Biochemistry

authors： Kundu N,Verma P,Kumar A,Dhar V,Dutta S,Chattopadhyay K

更新日期：2020-02-04 00:00:00

abstract：:The transfer of cytochrome P-450 from bovine adrenocortical submitochondrial particles (smp) to unilamellar liposome membranes was investigated using a table top ultracentrifuge. Submitochondrial particles were incubated with liposome membranes at 25 degrees C and precipitated by ultracentrifugation at 200000g for a f...

journal_title：Biochemistry

authors： Kominami S,Onizuka M,Tasaka-Marumoto C,Takemori S

更新日期：1995-04-11 00:00:00

abstract：:The phosphocarrier protein HPr of the phosphoenolpyruvate-dependent sugar transport system of Escherichia coli can exist in a phosphorylated and a nonphosphorylated form. During phosphorylation, the phosphoryl group is carried on a histidine residue, His15. The hydrogen-bonding state of this histidine was examined wit...

journal_title：Biochemistry

authors： van Dijk AA,de Lange LC,Bachovchin WW,Robillard GT

更新日期：1990-09-04 00:00:00

abstract：:Dodecameric glutamine synthetase (GS) from bacteria is formed from two face-to-face hexameric rings of identical subunits. These highly symmetrical aggregates from some bacteria, including Escherichia coli, "stack" in the presence of Zn2+ and other divalent ions to generate protein tubes (phase I) and subsequently ass...

journal_title：Biochemistry

authors： Yanchunas J Jr,Dabrowski MJ,Schurke P,Atkins WM

更新日期：1994-12-20 00:00:00

abstract：:Thioltransferase activity was identified and the enzyme purified to apparent homogeneity from human red blood cells. Activity was measured as glutathione-dependent reduction of the prototype substrate hydroxyethyl disulfide; formation of oxidized glutathione (GSSG) was coupled to NADPH oxidation by GSSG reductase (1 u...

journal_title：Biochemistry

authors： Mieyal JJ,Starke DW,Gravina SA,Dothey C,Chung JS

更新日期：1991-06-25 00:00:00

abstract：:Bacteriophage T7 RNA polymerase is a single-subunit enzyme which has a C-terminal amino acid sequence of Phe-Ala-Phe-Ala883 (FAFA883). Closely related hydrophobic sequences are present at the C termini of seven other single-subunit RNA polymerases, including the mitochondrial RNA polymerase. Mutations at any of the fo...

journal_title：Biochemistry

authors： Gardner LP,Mookhtiar KA,Coleman JE

更新日期：1997-03-11 00:00:00

abstract：:Upon cleavage of the reactive thioester bonds (Cys-949-Glx-952) of tetrameric human alpha 2-macroglobulin (alpha 2M) by methylamine, one sulfhydryl group per alpha 2M subunit is exposed. These identical sulfhydryl group sites were labeled with the thiol-specific nitroxide spin-labels (1-oxy-2,2,5,5-tetramethyl-3-pyrro...

journal_title：Biochemistry

authors： Zhao BL,Musci G,Sugawara Y,Berliner LJ

更新日期：1988-07-12 00:00:00

abstract：:While the iron-molybdenum cofactor (FeMoco) of nitrogenase, a constituent of the active site for nitrogen reduction, can be extracted into N-methylformamide (NMF) and pyrrollidinone, the inability to solubilize it in any other organic solvents has hampered further understanding of its structure and chemical properties...

journal_title：Biochemistry

authors： Wink DA,McLean PA,Hickman AB,Orme-Johnson WH

更新日期：1989-11-28 00:00:00

abstract：:Comparative mutagenesis studies of N-(2'-deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-AAF) and N-(2'-deoxyguanosin-8-yl)-2-aminofluorene (dG-AF) adducts positioned in the Nar I restriction enzyme site were performed using Escherichia coli (E. coli) and simian kidney (COS-7) cells. Oligodeoxynucleotides ((5)(')TCCTCG(...

journal_title：Biochemistry

authors： Tan X,Suzuki N,Grollman AP,Shibutani S

更新日期：2002-12-03 00:00:00

abstract：:Homoserine kinase, an enzyme in the aspartate pathway of amino acid biosynthesis in Escherichia coli, catalyzes the conversion of L-homoserine to L-homoserine phosphate. This enzyme has been found to have broad substrate specificity, including the phosphorylation of L-homoserine analogs where the carboxyl functional g...

journal_title：Biochemistry

authors： Huo X,Viola RE

更新日期：1996-12-17 00:00:00