Light-activated calcium release from sonicated bovine retinal rod outer segment disks.

abstract：:The thermotropic behavior of purified human complement protein C9 was investigated by high-sensitivity differential scanning calorimetry. When dissolved in physiological buffers (pH 7.2, 150 mM NaCl), C9 underwent three endothermic transitions with transition temperatures (Tm) centered at about 32, 48, and 53 degrees ...

journal_title：Biochemistry

authors： Lohner K,Esser AF

更新日期：1991-07-02 00:00:00

abstract：:Transcarboxylase (TC) from Propionibacterium shermanii, a biotin-dependent enzyme, catalyzes the transfer of a carboxyl group from methylmalonyl-CoA to pyruvate to form propionyl-CoA and oxalacetate. Within the multi-subunit enzyme complex, the 1.3S subunit functions as the carboxyl group carrier and also binds the ot...

journal_title：Biochemistry

authors： Reddy DV,Shenoy BC,Carey PR,Sönnichsen FD

更新日期：2000-03-14 00:00:00

abstract：:The blood-clotting protein fibrinogen is composed of three subunits, designated A alpha, B beta, and gamma, which are encoded by a family of related genes. As part of the acute-phase response, expression of the fibrinogen genes is coordinately regulated in the liver by glucocorticoids. To understand the factors underl...

journal_title：Biochemistry

authors： Roberts LR,Nichols LA,Holland LJ

更新日期：1993-11-02 00:00:00

abstract：:The thermodynamics and kinetics of magnesium binding to tRNA(Phe)(yeast) have been studied directly by 25Mg NMR. In 0.17 M Na+(aq), tRNA(Phe) exists in its native conformation and the number of strong binding sites (Ka greater than or equal to 10(4)) was estimated to be 3-4 by titration experiments, in agreement with ...

journal_title：Biochemistry

authors： Reid SS,Cowan JA

更新日期：1990-06-26 00:00:00

abstract：:Carbohydrate-binding module (CBM) family 13 includes the "R-type" or "ricin superfamily" beta-trefoil lectins. The C-terminal CBM, CBM13, of xylanase 10A from Streptomyces lividans is a family 13 CBM that is not only structurally similar to the "R-type" lectins but also somewhat functionally similar. The primary funct...

journal_title：Biochemistry

authors： Notenboom V,Boraston AB,Williams SJ,Kilburn DG,Rose DR

更新日期：2002-04-02 00:00:00

abstract：:Azotobacter vinelandii bacterioferritin (AvBF) containing 800-1500 Co or Mn atoms as Co(III) and Mn(III) oxyhydroxide cores (Co-AvBF, Mn-AvBF) was synthesized by the same procedure used previously for horse spleen ferritin (HoSF). The kinetics of reduction of Co-AvBF and Mn-AvBF by ascorbic acid are first-order in eac...

journal_title：Biochemistry

authors： Zhang B,Harb JN,Davis RC,Choi S,Kim JW,Miller T,Chu SH,Watt GD

更新日期：2006-05-09 00:00:00

abstract：:The cytidine repressor (CytR) is a member of the LacR family of bacterial repressors with distinct functional features. The Escherichia coli CytR regulon comprises nine operons whose palindromic operators vary in both sequence and, most significantly, spacing between the recognition half-sites. This suggests a strong ...

journal_title：Biochemistry

authors： Moody CL,Tretyachenko-Ladokhina V,Laue TM,Senear DF,Cocco MJ

更新日期：2011-08-09 00:00:00

abstract：:We have previously shown that introduction of an engineered Met160 residue in ascorbate peroxidase (S160M variant) leads to the formation of a covalent link between Met160 and the heme vinyl group [Metcalfe, C. L., et al. (2004) J. Am. Chem. Soc. 126, 16242-16248]. In this work, we have used electronic spectroscopy, H...

journal_title：Biochemistry

authors： Pipirou Z,Bottrill AR,Svistunenko DA,Efimov I,Basran J,Mistry SC,Cooper CE,Raven EL

更新日期：2007-11-20 00:00:00

abstract：:L-alpha-Hydroxy acid oxidase (listed as EC 1.4.3.2, L-amino acid: O2 oxidoreductase) has been purified 100-fold from rat kidney to apparent homogeneity by gel electrophoresis. A subunit molecular weight of 47,500 was found by sodium dodecyl sulfate gel electrophoresis, but in contrast to previous reports, the enzyme h...

journal_title：Biochemistry

authors： Cromartie TH,Walsh CT

更新日期：1975-06-17 00:00:00

abstract：:CTP synthetase [EC 6.3.4.2, UTP:ammonia ligase (ADP-forming)] from the yeast Saccharomyces cerevisiae catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to the C-4 position of UTP to form CTP. In this work, we demonstrated that CTP synthetase utilized dUTP as a substrate to synthesize dCTP. The ...

journal_title：Biochemistry

authors： Pappas A,Park TS,Carman GM

更新日期：1999-12-14 00:00:00

abstract：:In protein, conformational changes are often crucial for function but not easy to observe. Two functionally relevant conformational intermediate states of photosynthetic reaction center protein (RCs) are trapped and characterized at low temperature. RCs frozen in the dark do not allow electron transfer from the reduce...

journal_title：Biochemistry

authors： Xu Q,Gunner MR

更新日期：2001-03-13 00:00:00

abstract：:A cyclic nucleotide phosphodiesterase was extensively purified from the 100000g supernatant fraction of human platelets. The purification was 2500-3000-fold with 30% recovery of activity. The enzyme was isolated by DEAE-cellulose chromatography followed by adsorption to blue dextran-Sepharose and elution with cAMP. Th...

journal_title：Biochemistry

authors： Grant PG,Colman RW

更新日期：1984-04-10 00:00:00

abstract：:The structure of the adduct formed by reaction of acetaldehyde and Met5-enkephalin has been determined by analysis of 400-MHz proton spectra: two-dimensional J spectroscopy was used to resolve and measure virtually all the overlapping resonances, and decoupling difference spectroscopy was used to assign the resonances...

journal_title：Biochemistry

authors： Gidley MJ,Hall LD,Sanders JK,Summers MC

更新日期：1981-06-23 00:00:00

abstract：:The crystal structure of a human carbonic anhydrase II (CAII) variant, cis-proline-202-->alanine (P202A), has been determined at 1.7-A resolution, indicating that the wild-type geometry, including the cis-peptidyl linkage, is retained upon substitution of proline by alanine. The CO2 hydrase activity and affinity for s...

journal_title：Biochemistry

authors： Tweedy NB,Nair SK,Paterno SA,Fierke CA,Christianson DW

更新日期：1993-10-19 00:00:00

abstract：:Genomic clones encompassing all the translated sequences, the 3' untranslated sequence, and 1 kb flanking the ATG translation initiation codon of bovine tropoelastin have been obtained and characterized by restriction enzyme analysis and extensive DNA sequencing. These analyses demonstrated that functionally distinct ...

journal_title：Biochemistry

authors： Yeh H,Anderson N,Ornstein-Goldstein N,Bashir MM,Rosenbloom JC,Abrams W,Indik Z,Yoon K,Parks W,Mecham R

更新日期：1989-03-21 00:00:00

abstract：:Initial rate kinetic studies and reduced coenzyme binding studies with bovine glutamate dehydrogenase have shown that an enzyme-NAD(P)H-glutamate abortive complex is a major participant in the overall enzyme-catalyzed reaction. The allosteric regulator ADP is shown to activate the enzyme by destabilizing this abortive...

journal_title：Biochemistry

authors： George A,Bell JE

更新日期：1980-12-23 00:00:00

abstract：:The binding of the benzo[a]pyrene metabolite anti-BPDE (r7, t8-dihydroxy-t9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene) to the N(2) group of 2'-deoxyguanosine residues (dG) is known to adversely affect the Michaelis-Menten primer extension kinetics catalyzed by DNA Pol I and other polymerases. In this work, the impact...

journal_title：Biochemistry

authors： Rechkoblit O,Amin S,Geacintov NE

更新日期：1999-09-07 00:00:00

abstract：:The interaction of pig plasma gelsolin (G) and actin (A) was examined by using photoreactive 4-maleimidobenzophenone-actin (BPM-actin) in which BPM was previously conjugated to Cys-374 of actin through the maleimide moiety. In the presence of micromolar [Ca2+], the major cross-linked product observed after irradiation...

journal_title：Biochemistry

authors： Doi YK,Banba M,Vertut-Doï A

更新日期：1991-06-11 00:00:00

abstract：:Site-directed spin labeling (SDSL), the site-specific incorporation of nitroxide spin-labels into a protein, has allowed us to investigate ligand-induced conformational changes in the ligand-binding domain of human estrogen receptor alpha (hERalpha-LBD). EPR (electron paramagnetic resonance) spectroscopy of the nitrox...

journal_title：Biochemistry

authors： Hurth KM,Nilges MJ,Carlson KE,Tamrazi A,Belford RL,Katzenellenbogen JA

更新日期：2004-02-24 00:00:00

abstract：:To better understand the factors controlling the binding of apolipoprotein molecules at the surfaces of serum lipoprotein particles, the adsorption of human apolipoprotein A-I to phospholipid monolayers has been studied. The influence of lipid packing was investigated by spreading the monolayers at various initial sur...

journal_title：Biochemistry

authors： Ibdah JA,Phillips MC

更新日期：1988-09-06 00:00:00

abstract：:In the putative secondary structure of the transposon Tn10-encoded metal-tetracycline/H+ antiporter [Yamaguchi, et al. (1992) J. Biol. Chem. 267, 7490-7498], Tyr50-XXX-Gln54 in transmembrane helix 2 and Gly80-XXX-Asp84 in helix 3 are thought to face each other in the N-terminal region. At the corresponding positions i...

journal_title：Biochemistry

authors： Yamaguchi A,Akasaka T,Kimura T,Sakai T,Adachi Y,Sawai T

更新日期：1993-06-01 00:00:00

abstract：:Rett syndrome causing missense mutations in the methyl-CpG-binding domain (MBD) of methyl CpG-binding protein 2 (MeCP2) were investigated both in silico and in vitro to reveal their effect on protein stability. It is demonstrated that the vast majority of frequently occurring mutations in the human population indeed a...

journal_title：Biochemistry

authors： Kucukkal TG,Yang Y,Uvarov O,Cao W,Alexov E

更新日期：2015-10-20 00:00:00

abstract：:Structures of several voltage-gated sodium (NaV) channels from bacteria have been determined recently, but the same feat might not be achieved for the mammalian counterparts in the near future. Thus, at present, computational studies of the mammalian NaV channels have to be performed using homology models based on the...

journal_title：Biochemistry

authors： Patel D,Mahdavi S,Kuyucak S

更新日期：2016-03-29 00:00:00

abstract：:When rabbits are fed a diet supplemented with cholesterol, their plasma cholesterol levels increase markedly, and they develop atherosclerosis. Most of the plasma cholesterol exists as cholesteryl esters in very low density lipoproteins (VLDL) and intermediate-density lipoproteins (IDL). The triglyceride content of th...

journal_title：Biochemistry

authors： Kroon PA,Seidenberg J

更新日期：1982-12-07 00:00:00

abstract：:Aldehyde dehydrogenase 7A1 (ALDH7A1) is part of lysine catabolism and catalyzes the NAD(+)-dependent oxidation of α-aminoadipate semialdehyde to α-aminoadipate. Herein, we describe a structural study of human ALDH7A1 focused on substrate recognition. Five crystal structures and small-angle X-ray scattering data are re...

journal_title：Biochemistry

authors： Luo M,Tanner JJ

更新日期：2015-09-08 00:00:00

abstract：:To gain insight into the folding of large proteins, we constructed a bovine beta-lactoglobulin (beta-lg) dimeric mutant, A34C/C121A beta-lg. In the mutant, a free thiol group of wild-type beta-lg at Cys121 was removed and two beta-lg molecules were linked by a disulfide bridge through Cys34 created at the dimer's inte...

journal_title：Biochemistry

authors： Yagi M,Kameda A,Sakurai K,Nishimura C,Goto Y

更新日期：2008-06-03 00:00:00

abstract：:As discovered by Grazi & Trombetta [Grazi, E., & Trombetta, G. (1978) Biochem. J. 175, 361], fructose-1,6-bisphosphate aldolase of rabbit muscle causes the slow formation of inorganic phosphate (Pi) and methylglyoxal when incubated with dihydroxyacetone phosphate (DHAP). In addition, these authors found an acid-labile...

journal_title：Biochemistry

authors： Iyengar R,Rose IA

更新日期：1981-03-03 00:00:00

abstract：:The role of the amino-terminal region of alpha-striated muscle tropomyosin has been analyzed by reconstituting thin filaments with a version of the protein lacking the first six amino acids. While Omp T-digested tropomyosin (residues 7-284) does not bind significantly to F-actin at micromolar concentrations, an intera...

journal_title：Biochemistry

authors： Goonasekara CL,Heeley DH

更新日期：2009-04-21 00:00:00

abstract：:Manganese peroxidase (MnP) from Phanerochaete chrysosporium undergoes a pH-dependent conformational change evidenced by changes in the electronic absorption spectrum. This high- to low-spin alkaline transition occurs at approximately 2 pH units lower in an F190I mutant MnP when compared to the wild-type enzyme. Herein...

journal_title：Biochemistry

authors： Youngs HL,Moënne-Loccoz P,Loehr TM,Gold MH

更新日期：2000-08-15 00:00:00

abstract：:The low-frequency FeCN vibrations of cyanoferric myeloperoxidase (MPO) and horseradish peroxidase (HRP) have been measured by resonance Raman spectroscopy. The ordering of the frequencies of the predominantly FeC stretching and FeCN bending normal vibrational modes in the two peroxidases differs. These normal mode vib...

journal_title：Biochemistry

authors： López-Garriga JJ,Oertling WA,Kean RT,Hoogland H,Wever R,Babcock GT

更新日期：1990-10-09 00:00:00