Acetaldehyde--enkephalins: structure proof and some conformational deductions from one- and two-dimensional proton nuclear magnetic resonance spectra.

abstract：:X-ray absorption spectra are reported for the multi-Cu oxidase Rhus vernicifera laccase in oxidized and fully reduced forms and for laccase from which the type 2 Cu has been depleted (T2D). The structure of the Cu K edge for both preparations shows the presence of CuII and CuI in the oxidized and reduced states, respe...

journal_title：Biochemistry

authors： Woolery GL,Powers L,Peisach J,Spiro TG

更新日期：1984-07-17 00:00:00

abstract：:The ionization and phase behavior of 1,2-dipalmitoyl-sn-glycero-3-phosphoserine have been investigated under a variety of condtions by several different methods. As measured by turbidity changes, the temperature of the crystal-liquid crystal phase transition of this lipid is influenced by pH and mono- and divalent ca...

journal_title：Biochemistry

authors： MacDonald RC,Simon SA,Baer E

更新日期：1976-02-24 00:00:00

abstract：:Pseudokinases play important roles in signal transduction and cellular processes similar to those of catalytically competent kinases. However, pseudokinase pharmacological tractability and conformational space accessibility are poorly understood. Pseudokinases have only recently been suggested to adopt "inactive" conf...

journal_title：Biochemistry

authors： Smith RHB,Khan ZM,Ung PM,Scopton AP,Silber L,Mack SM,Real AM,Schlessinger A,Dar AC

更新日期：2021-01-13 00:00:00

abstract：:Recently, we revealed the architecture of the clamp-loader-helicase (tau-DnaB) complex in Bacillus by atomic force microscopy imaging and constructed a structural model, whereby a pentameric clamp-loader interacts with the hexameric helicase. Crucial to this model is the assumption that the clamp-loader forms a pentam...

journal_title：Biochemistry

authors： Haroniti A,Till R,Smith MC,Soultanas P

更新日期：2003-09-23 00:00:00

abstract：:Human apolipoprotein stimulation of sphingomyelin (SM) hydrolysis by sphingomyelinase from human skin fibroblasts has been studied. Apolipoproteins A-I, A-II, B, C-I, and E do not enhance sphingomyelin hydrolysis above control levels. In contrast, apoC-II stimulates sphingomyelin hydrolysis by approximately 2.5-fold. ...

journal_title：Biochemistry

authors： Ahmad TY,Beaudet AL,Sparrow JT,Morrisett JD

更新日期：1986-07-29 00:00:00

abstract：:We report here that polyamide nucleic acid (PNA) as well as a polyamide nucleic acid-DNA chimera complementary to the primer binding site of the HIV-1 genome can completely block priming by tRNA3Lys and consequently the in vitro initiation of reverse transcription by HIV-1 RT. Conventional heating and cooling is not r...

journal_title：Biochemistry

authors： Lee R,Kaushik N,Modak MJ,Vinayak R,Pandey VN

更新日期：1998-01-20 00:00:00

abstract：:The continued rise of antibiotic-resistant infections coupled with the limited pipeline of new antimicrobials highlights the pressing need for the development of new antibacterial agents. One potential pathway for new agents is de novo purine biosynthesis as studies have shown that bacteria and lower eukaryotes synthe...

journal_title：Biochemistry

authors： Streeter CC,Lin Q,Firestine SM

更新日期：2019-04-30 00:00:00

abstract：:Numerous studies have been published stressing the importance of finding ligands that can bind specifically to DNA secondary structures. Several have identified ligands that are presented as having specific binding to the G-quadruplex; however, these were not originally tested on the complementary i-motif structure. T...

journal_title：Biochemistry

authors： Abdelhamid MAS,Gates AJ,Waller ZAE

更新日期：2019-01-29 00:00:00

abstract：:The crystal structure of ferredoxin-NADP+ reductase (FNR) suggests that Ser96 is directly involved in hydride transfer between the isoalloxazine moiety of FAD and the nicotinamide ring of NADP(H). To probe its role, Ser96 has been mutated to valine (S96V) and glycine (S96G). These mutations primarily affected the inte...

journal_title：Biochemistry

authors： Aliverti A,Bruns CM,Pandini VE,Karplus PA,Vanoni MA,Curti B,Zanetti G

更新日期：1995-07-04 00:00:00

abstract：:We examined the metabolism of exogenously added 13C-labeled fructose 1,6-bisphosphate (either labeled at the first and sixth carbons or labeled at the first carbon only) and of [2-13C]glucose in well-oxygenated and well-superfused hog carotid artery segments. Exogenously added fructose 1,6-bisphosphate was utilized by...

journal_title：Biochemistry

authors： Hardin CD,Roberts TM

更新日期：1995-01-31 00:00:00

abstract：:Alpha2u-globulin is a male rat liver protein under multihormonal control which represents approximately 1% of hepatic protein synthesis. We have measured the rate of transcription of the alpha2u-globulin gene using a nuclear cell-free transcriptional system with mercurated CTP as substrate for the endogenous RNA polym...

journal_title：Biochemistry

authors： Chan KM,Kurtz DT,Feigelson P

更新日期：1978-07-25 00:00:00

abstract：:Vanadate can often bind to phosphoryl transfer enzymes to form a trigonal-bipyramidal structure at the active site. The enzyme-vanadate dissociation constants in these enzymes are much lower than those for phosphate. Therefore, enzyme-bound vanadate moieties are often considered as transition state analogues. To test ...

journal_title：Biochemistry

authors： Deng H,Callender R,Huang Z,Zhang ZY

更新日期：2002-05-07 00:00:00

abstract：:Using proton magnetic resonance, we have investigated imino and amino proton exchange in the Z form of the four oligomers d(Cbr8GCGCbr8G), d(CGm5CGCG), d(CG)6, and d(CG)12. In the latter two oligomers, all five exchangeable protons have been assigned. We find that proton acceptors such as NH3 or the basic form of Tris...

journal_title：Biochemistry

authors： Kochoyan M,Leroy JL,Guéron M

更新日期：1990-05-22 00:00:00

abstract：:A new fluorescent lipid analogue, bimanephosphatidylcholine, has been synthesized for use in lipid bilayers. This probe is well suited as an energy-transfer donor with N-(7-nitro-2,1,3-benzoxadiazol-4-yl)phosphatidylethanolamine as the acceptor. Dimyristoylphosphatidylethanolamine vesicles are prepared by sonication a...

journal_title：Biochemistry

authors： Pryor C,Bridge M,Loew LM

更新日期：1985-04-23 00:00:00

abstract：:Inwardly rectifying potassium channels are important in cellular repolarization of many excitable tissues. Amino acid sequence alignment of different mammalian inward rectifier K(+) channels revealed two absolutely conserved cysteine residues in the putative extracellular face, suggesting a possible disulfide bond. Re...

journal_title：Biochemistry

authors： Cho HC,Tsushima RG,Nguyen TT,Guy HR,Backx PH

更新日期：2000-04-25 00:00:00

abstract：:In eukaryotes, a major route for ATP-dependent protein breakdown proceeds through covalent intermediates of target proteins destined for degradation and the highly conserved, 76 amino acid protein ubiquitin. In rabbit reticulocytes, it has been shown that hemin effectively inhibits this pathway by blocking the catabol...

journal_title：Biochemistry

authors： Vierstra RD,Sullivan ML

更新日期：1988-05-03 00:00:00

abstract：:The fluorescent pH indicator 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) was used to investigate changes in the intracellular pH (pHi) of individual Sf9 cells in response to changes in the composition of the external medium. Under standard conditions, the resting pHi was 0.2-0.3 unit lower than the extracel...

journal_title：Biochemistry

authors： Vachon V,Paradis MJ,Marsolais M,Schwartz JL,Laprade R

更新日期：1995-11-21 00:00:00

abstract：:The chemical mechanism of phosphodiester bond hydrolysis catalyzed by a junction-resolving enzyme has been investigated. Endonuclease I of phage T7 is a member of the nuclease superfamily of proteins that include many restriction enzymes, and the structure of the active site is very similar to that of BglI in particul...

journal_title：Biochemistry

authors： Liu J,Déclais AC,Lilley DM

更新日期：2006-03-28 00:00:00

abstract：:The expression level of protein DR1199 is observed to increase considerably in the radio-resistant bacterium Deinococcus radiodurans following irradiation. This protein belongs to the DJ-1 superfamily, which includes proteins with diverse functions, such as the archaeal proteases PhpI and PfpI, the bacterial chaperone...

journal_title：Biochemistry

authors： Fioravanti E,Durá MA,Lascoux D,Micossi E,Franzetti B,McSweeney S

更新日期：2008-11-04 00:00:00

abstract：:Human angiotensin-converting enzyme (ACE) has two homologous domains, the N and C domains, with differing substrate preferences. X-ray crystal structures of the C and N domains complexed with various inhibitors have allowed identification of active site residues that might be important for the molecular basis of this ...

journal_title：Biochemistry

authors： Watermeyer JM,Kröger WL,O'Neill HG,Sewell BT,Sturrock ED

更新日期：2008-06-03 00:00:00

abstract：:We review signaling by reactive oxygen species, which is emerging as a major physiological process. However, among the reactive oxygen species, H(2)O(2) best fulfills the requirements of being a second messenger. Its enzymatic production and degradation, along with the requirements for the oxidation of thiols by H(2)O...

journal_title：Biochemistry

authors： Forman HJ,Maiorino M,Ursini F

更新日期：2010-02-09 00:00:00

abstract：:The 24-kDa subunit of mitochondrial NADH-ubiquinone reductase (complex I) is an iron-sulfur protein that is present in the flavoprotein or NADH dehydrogenase II subcomplex. It is a nuclear gene product and is imported into the organelle. A group of human patients with mitochondrial myopathy have been shown to have red...

journal_title：Biochemistry

authors： Pilkington SJ,Walker JE

更新日期：1989-04-18 00:00:00

abstract：:The preferential interactions of proteins with solvent components were studied in concentrated aqueous solutions of the sulfate, acetate, and chloride salts of Mg2+, Ba2+, Ca2+, Mn2+ and Ni2+ [except for CaSO4, BaSO4, Mn-(OAc)2, and Ni(OAc)2], and results were compared with those of the Na+ salts. It was found that, f...

journal_title：Biochemistry

authors： Arakawa T,Timasheff SN

更新日期：1984-12-04 00:00:00

abstract：:PI-SceI is a member of an unusual class of rare cutting homing endonucleases produced by an autocatalytic protein splicing from a precursor. To analyze the structural and functional domain organization of the endonuclease PI-SceI and to examine whether the DNA binding activity can be structurally separated from the ca...

journal_title：Biochemistry

authors： Pingoud V,Grindl W,Wende W,Thole H,Pingoud A

更新日期：1998-06-02 00:00:00

abstract：:Ultrahigh-resolution X-ray diffraction data from cryo-cooled, B. lentus subtilisin crystals has been collected to a resolution of 0.78 A. The refined model coordinates have a rms deviation of 0.22 A relative to the same structure determined at room temperature and 2.0 A resolution. Several regions of main-chain and si...

journal_title：Biochemistry

authors： Kuhn P,Knapp M,Soltis SM,Ganshaw G,Thoene M,Bott R

更新日期：1998-09-29 00:00:00

abstract：:Pharaonis halorhodopsin (phR), the light-driven chloride ion pump from Natronobacterium pharaonis with C-terminal histidine tag, was expressed in Escherichia coli cells. The protein was solubilized with 0.1% n-dodecyl beta-D-maltopyranoside and purified with a nickel column. Removal of Cl- from the medium yields blue ...

journal_title：Biochemistry

authors： Sato M,Kanamori T,Kamo N,Demura M,Nitta K

更新日期：2002-02-19 00:00:00

abstract：:Escherichia coli tyrosine kinase (Etk) regulates the export of pathogenic capsular polysaccharide (CPS) by intermolecularly autophosphorylating its C-terminal tyrosine cluster. The kinase Etk, however, needs to be first activated by the intramolecular phosphorylation of a tyrosine residue, Y574, next to the active sit...

journal_title：Biochemistry

authors： Lu T,Tan H,Lee D,Chen G,Jia Z

更新日期：2009-08-25 00:00:00

abstract：:Triton X-100 solubilized monomers of bacteiorhodopsin (bR) show a decrease in the extent of light adaptation; the red shift and the absorbance increase of the visible absorption band are reduced no less than half the values observed in purple membrane (p.m.) with a corresponding reduction in the isomerization of 13-ci...

journal_title：Biochemistry

authors： Casadio R,Stoeckenius W

更新日期：1980-07-08 00:00:00

abstract：:Cyanobacteriochromes (CBCRs) are cyanobacterial photoreceptors distantly related to phytochromes. Like phytochromes, CBCRs photointerconvert between two photostates that accompany photoisomerization of their bilin chromophores. While phytochromes typically exhibit red/far-red photocycles, CBCR photocycles are much mor...

journal_title：Biochemistry

authors： Gottlieb SM,Kim PW,Corley SC,Madsen D,Hanke SJ,Chang CW,Rockwell NC,Martin SS,Lagarias JC,Larsen DS

更新日期：2014-02-18 00:00:00

abstract：:The N-type voltage-gated Ca(2+) channel CaV2.2 is one of the important targets for pain management. ω-Conotoxins isolated from venoms of cone snails, which specifically inhibit CaV2.2, are promising scaffolds for novel analgesics. The inhibitory action of ω-conotoxins on CaV2.2 has been examined experimentally, but th...

journal_title：Biochemistry

authors： Chen R,Chung SH

更新日期：2013-05-28 00:00:00