Effect of protein-protein interaction on light adaptation of bacteriorhodopsin.

Abstract:

:Triton X-100 solubilized monomers of bacteiorhodopsin (bR) show a decrease in the extent of light adaptation; the red shift and the absorbance increase of the visible absorption band are reduced no less than half the values observed in purple membrane (p.m.) with a corresponding reduction in the isomerization of 13-cis- to all-trans-retinal. Cross-linking of bR with glutaraldehyde before exposure to Triton prevents dissociation of the lattice and reduction in light adaptation. Experiments with cross-linked and lipid-extracted p.m. show that Triton effectively substitutes for the native membrane lipids and that the lattice structure apparently stabilizes the light-adapted state of bR under illumination. In lipid vesicles at molar lipid protein ratios greater than or equal to 80, bR exists as monomers above the lipid-phase transition and aggregates below the phase transition. Above the lipid-phase transition and aggregates below the phase transition. Above the lipid-phase transition light adaptation in the monomers, measured as either the red shift of the visible absorbance maximum or the isomerizaiton o 13-cis- to all-trans-retinal, is also reduced to less than half of the extent observed in intact purple membrane or in the bR aggregates formed in lipid vesicles below the plhase transition. At very high lipid to protein ratios, bR molecules cannot aggregate when the temperature is decreased below the phase transition, and these monomers in a solid lipid phase show the same reduced extent of light adaptation as monomers above the phase transition, thus confirming that this effect is mainly due to the absence of protein-protein interaction and not to the state of the lipid. The extent of the red shift upon light adaptation may be used as a convenient indicator to distinguish the aggregated and monomeric states of bR.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Casadio R,Stoeckenius W

doi

10.1021/bi00555a043

subject

Has Abstract

pub_date

1980-07-08 00:00:00

pages

3374-81

issue

14

eissn

0006-2960

issn

1520-4995

journal_volume

19

pub_type

杂志文章
  • Conserved phosphoprotein interaction motif is functionally interchangeable between ataxin-7 and arrestins.

    abstract::Olivopontocerebellar atrophy with retinal degeneration is a hereditary neurodegenerative disorder that belongs to the subtype II of the autosomal dominant cerebellar ataxias and is characterized by early-onset cerebellar and macular degeneration preceded by diagnostically useful tritan colorblindness. The gene mutated...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi992694y

    authors: Mushegian AR,Vishnivetskiy SA,Gurevich VV

    更新日期:2000-06-13 00:00:00

  • Membrane binding kinetics of protein kinase C betaII mediated by the C2 domain.

    abstract::Conventional isoforms of protein kinase C (PKC) are activated when their two membrane-targeting modules, the C1 and C2 domains, bind the second messengers diacylglycerol (DG) and Ca2+, respectively. This study investigates the mechanism of Ca2+-induced binding of PKC betaII to anionic membranes mediated by the C2 doma...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi010761u

    authors: Nalefski EA,Newton AC

    更新日期:2001-11-06 00:00:00

  • Post-translational modifications of aquaporin 0 (AQP0) in the normal human lens: spatial and temporal occurrence.

    abstract::Because of the lack of protein turnover in fiber cells of the ocular lens, Aquaporin 0 (AQP0), the most abundant membrane protein in the lens, undergoes extensive post-translational modification with fiber cell age. To map the distribution of modified forms of AQP0 within the lens, normal human lenses ranging in age f...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0496034

    authors: Ball LE,Garland DL,Crouch RK,Schey KL

    更新日期:2004-08-03 00:00:00

  • Engineering of the nonspecific phospholipase C from Bacillus cereus: replacement of glutamic acid-4 by alanine results in loss of interfacial catalysis and enhanced phosphomonoesterase activity.

    abstract::The nonspecific phospholipase C from Bacillus cereus is a zinc metalloenzyme that catalyzes the hydrolysis of phospholipids to yield diacylglycerol and a phosphate monoester. Glu-4 has been proposed as a potential candidate for the general base in the hydrolysis reaction and was shown to interact with the substrate he...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi972751s

    authors: Tan CA,Roberts MF

    更新日期:1998-03-24 00:00:00

  • Rhodopsin in dimyristoylphosphatidylcholine-reconstituted bilayers forms metarhodopsin II and activates Gt.

    abstract::The photochemical intermediate metarhodopsin II (meta II; lambda max = 380 nm) is generally identified with rho*, the conformation of photolyzed rhodopsin which binds and activates the visual G-protein, Gt [Emeis, D., & Hoffman, K.P. (1981) FEBS Lett. 136, 201-207]. Purified bovine rhodopsin was incorporated into vesi...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00215a006

    authors: Mitchell DC,Kibelbek J,Litman BJ

    更新日期:1991-01-08 00:00:00

  • Histidine 55 of tryptophan 2,3-dioxygenase is not an active site base but regulates catalysis by controlling substrate binding.

    abstract::Tryptophan 2,3-dioxygenase (TDO) from Xanthomonas campestris is a highly specific heme-containing enzyme from a small family of homologous enzymes, which includes indoleamine 2,3-dioxygenase (IDO). The structure of wild type (WT TDO) in the catalytically active, ferrous (Fe (2+)) form and in complex with its substrate...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi801202a

    authors: Thackray SJ,Bruckmann C,Anderson JL,Campbell LP,Xiao R,Zhao L,Mowat CG,Forouhar F,Tong L,Chapman SK

    更新日期:2008-10-07 00:00:00

  • Through the Lipopolysaccharide Glass: A Potent Antimicrobial Peptide Induces Phase Changes in Membranes.

    abstract::In the following, molecular simulations are used to reveal unexpected behavior within bacterial membranes. We show that lipopolysaccharide molecules found in these membranes form viscous amorphous solids when they are interlinked with monovalent and divalent cations. The bilayers exhibit both liquid and glassy charact...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.6b01063

    authors: Jefferies D,Hsu PC,Khalid S

    更新日期:2017-03-21 00:00:00

  • Probing structural differences between PrP(C) and PrP(Sc) by surface nitration and acetylation: evidence of conformational change in the C-terminus.

    abstract::We used two chemical modifiers, tetranitromethane (TNM) and acetic anhydride (Ac(2)O), which specifically target accessible tyrosine and lysine residues, respectively, to modify recombinant Syrian hamster PrP(90-231) [rSHaPrP(90-231)] and SHaPrP 27-30, the proteinase K-resistant core of PrP(Sc) isolated from brain of ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi102073j

    authors: Gong B,Ramos A,Vázquez-Fernández E,Silva CJ,Alonso J,Liu Z,Requena JR

    更新日期:2011-06-07 00:00:00

  • Acid-induced unfolding and refolding transitions of cytochrome c: a three-state mechanism in H2O and D2O.

    abstract::Whereas the salt-dependent conformational transition of acid-denatured horse ferricytochrome c at pH 2 is approximated by a two-state mechanism from the acid-unfolded state to the molten globule state [Kataoka, M., Hagihara, Y., Mihara, K., & Goto, Y. (1993) J. Mol. Biol. 229, 591-596], the corresponding transition in...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00095a017

    authors: Goto Y,Hagihara Y,Hamada D,Hoshino M,Nishii I

    更新日期:1993-11-09 00:00:00

  • Flash-induced relaxation changes of the EPR signals from the manganese cluster and YD reveal a light-adaptation process of photosystem II.

    abstract::By exposing photosystem II (PSII) samples to an incrementing number of excitation flashes at room temperature, followed by freezing, we could compare the Mn-derived multiline EPR signal from the S2 oxidation state as prepared by 1, 5, 10, and 25 flashes of light. While the S2 multiline signals exhibited by these sampl...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi026848c

    authors: Peterson S,Ahrling KA,Högblom JE,Styring S

    更新日期:2003-03-11 00:00:00

  • Inhibition of peptidyl-prolyl cis/trans isomerase activity by substrate analog structures: thioxo tetrapeptide-4-nitroanilides.

    abstract::The ubiquitous cyclophilins belong to peptidyl-prolyl cis/trans isomerases (PPIases; EC 5.2.1.8). They are able to catalyze the cis/trans isomerization about peptidyl-prolyl amide bonds. The mode of action of human cytosolic cyclophilin (Cyp18cy) has been studied on substrate analog tetrapeptide-4-nitroanilides contai...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00040a012

    authors: Schutkowski M,Wöllner S,Fischer G

    更新日期:1995-10-10 00:00:00

  • Molecular dynamics simulations of the unfolding of barnase in water and 8 M aqueous urea.

    abstract::Molecular dynamics simulations of barnase have been conducted both in water and in 8 M urea solution for 500 ps at 25 degrees C and for 2000 ps at 85 degrees C. The final structure of the aqueous simulation at room temperature matches closely the structure obtained by NMR and the experimentally observed protections fr...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi970096i

    authors: Tirado-Rives J,Orozco M,Jorgensen WL

    更新日期:1997-06-17 00:00:00

  • Thermal and urea-induced unfolding of the marginally stable lac repressor DNA-binding domain: a model system for analysis of solute effects on protein processes.

    abstract::Thermodynamic and structural evidence indicates that the DNA binding domains of lac repressor (lacI) exhibit significant conformational adaptability in operator binding, and that the marginally stable helix-turn-helix (HTH) recognition element is greatly stabilized by operator binding. Here we use circular dichroism a...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0270992

    authors: Felitsky DJ,Record MT Jr

    更新日期:2003-02-25 00:00:00

  • Comparison of the sequence-selective DNA binding by peptide dimers with covalent and noncovalent dimerization domains.

    abstract::Sequence-specific DNA binding proteins generally consist of more than two DNA-contacting regions to ensure the selectivity of recognition. The multiple DNA binding modules are connected either through the covalent linker or through the noncovalent dimerization domain. We have compared the DNA binding of peptide dimers...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi981743o

    authors: Aizawa Y,Sugiura Y,Morii T

    更新日期:1999-02-02 00:00:00

  • Proton transfer in carbonic anhydrase is controlled by electrostatics rather than the orientation of the acceptor.

    abstract::Combined quantum mechanical/molecular mechanical (QM/MM) simulations are carried out to analyze factors that dictate the proton transfer in carbonic anhydrase II (CAII), an enzyme that has been used as a prototypical example of long-range proton transfers in biomolecules. In contrast to the long-held conjecture in the...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi701950j

    authors: Riccardi D,König P,Guo H,Cui Q

    更新日期:2008-02-26 00:00:00

  • Human immunodeficiency virus-1 protease. 1. Initial velocity studies and kinetic characterization of reaction intermediates by 18O isotope exchange.

    abstract::The peptidolytic reaction of HIV-1 protease has been investigated by using four oligopeptide substrates, Ac-Ser-Gln-Asn-Tyr-Pro-Val-Val-NH2, Ac-Arg-Ala-Ser-Gln-Asn-Tyr-Pro-Val-Val-NH2, Ac-Ser-Gln-Ser-Tyr-Pro-Val-Val-NH2, and Ac-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly-NH2, that resemble two cleavage sites found within the natu...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00098a023

    authors: Hyland LJ,Tomaszek TA Jr,Roberts GD,Carr SA,Magaard VW,Bryan HL,Fakhoury SA,Moore ML,Minnich MD,Culp JS

    更新日期:1991-08-27 00:00:00

  • Mobilities of the inner three core residues and the Man(alpha 1--6) branch of the glycan at Asn78 of the alpha-subunit of human chorionic gonadotropin are restricted by the protein.

    abstract::Human chorionic gonadotropin (hCG) is a heterodimeric glycoprotein hormone involved in the maintenance of the corpus luteum in early pregnancy. The free alpha-subunit of hCG has a biological activity of its own, namely, stimulation of prolactin secretion from term pregnancy decidual cells [Blithe, D. L., et al. (1991)...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9718548

    authors: van Zuylen CW,de Beer T,Leeflang BR,Boelens R,Kaptein R,Kamerling JP,Vliegenthart JF

    更新日期:1998-02-17 00:00:00

  • Escherichia coli cystathionine gamma-synthase does not obey ping-pong kinetics. Novel continuous assays for the elimination and substitution reactions.

    abstract::Cystathionine gamma-synthase (CGS) is a pyridoxal phosphate-dependent enzyme that catalyzes a gamma-replacement reaction, in which the succinyl group of an O-succinyl-L-homoserine (L-OSHS) is displaced by the thiol of L-cysteine to form L-cystathionine, in the first step of the bacterial transsulfuration pathway. The ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi035107o

    authors: Aitken SM,Kim DH,Kirsch JF

    更新日期:2003-09-30 00:00:00

  • Electric field induced transient pores in phospholipid bilayer vesicles.

    abstract::A study of the voltage induction of transient pores in phospholipid bilayer vesicles is reported. Unilamellar vesicles (dipalmitoylphosphatidylcholine), with a size distribution of 100 +/- 30 nm, were prepared by the method of Enoch & Strittmatter [Enoch, H., & Strittmatter, P. (1979) Proc. Natl. Acad. Sci. U.S.A. 76,...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00509a022

    authors: Teissie J,Tsong TY

    更新日期:1981-03-17 00:00:00

  • Procatalytic ligand strain. Ionization and perturbation of 8-nitroxanthine at the urate oxidase active site.

    abstract::The binding of the inhibitor 8-nitroxanthine to urate oxidase has been investigated by Raman and UV-visible absorption spectroscopy. The absorption maximum of 8-nitroxanthine shifts from 380 to 400 nm upon binding to the enzyme, demonstrating that the electronic structure of the ligand is perturbed. It has been propos...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0507837

    authors: Doll C,Bell AF,Power N,Tonge PJ,Tipton PA

    更新日期:2005-08-30 00:00:00

  • Kinetics of hepatic cytochrome P-450 reduction: correlation with spin state of the ferric heme.

    abstract::The reduction kinetics of cytochrome P-450 are known to be biphasic, with a rapid initial phase and a slower subsequent phase, both of which appear linear in semilogarithmic plots. The present report demonstrates that these biphasic reduction kinetics can be described in terms of a preequilibrium between high- and low...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00535a034

    authors: Backes WL,Sligar SG,Schenkman JB

    更新日期:1982-03-16 00:00:00

  • Sp1-targeted inhibition of gene transcription by WP631 in transfected lymphocytes.

    abstract::The binding of Sp1 transcription factor to DNA is considered a potential target for small ligands designed to interfere with gene transcription. We attempted to distinguish the direct inhibition of the Sp1-binding to DNA in vivo (cell culture) from more indirect effects due to the network of pathways that modulate cel...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi036185e

    authors: Mansilla S,Priebe W,Portugal J

    更新日期:2004-06-15 00:00:00

  • Kinetic consequences of introducing a proximal selenocysteine ligand into cytochrome P450cam.

    abstract::The structural, electronic, and catalytic properties of cytochrome P450cam are subtly altered when the cysteine that coordinates to the heme iron is replaced with a selenocysteine. To map the effects of the sulfur-to-selenium substitution on the individual steps of the catalytic cycle, we conducted a comparative kinet...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.5b00939

    authors: Vandemeulebroucke A,Aldag C,Stiebritz MT,Reiher M,Hilvert D

    更新日期:2015-11-10 00:00:00

  • Identification of a 33-kilodalton cytoskeletal protein with high affinity for the sodium channel.

    abstract::The voltage-sensitive sodium channel is an intrinsic membrane protein that is nonrandomly distributed in neurons, suggesting a possible interaction with other cellular constituents. In this study, we have directly tested the hypothesis that components of the cytoskeleton interact with sodium channels. Utilizing the me...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00406a003

    authors: Edelstein NG,Catterall WA,Moon RT

    更新日期:1988-03-22 00:00:00

  • Exchange rates at the lipid-protein interface of myelin proteolipid protein studied by spin-label electron spin resonance.

    abstract::The electron spin resonance (ESR) spectra from spin-labeled phospholipids in recombinants of myelin proteolipid apoprotein with dimyristoylphosphatidylcholine have been simulated with the exchanged-coupled Bloch equations to obtain values for both the fraction of motionally restricted lipids and the exchange rate betw...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00401a009

    authors: Horváth LI,Brophy PJ,Marsh D

    更新日期:1988-01-12 00:00:00

  • Intestinal absorption of dietary cholesteryl ester is decreased but retinyl ester absorption is normal in carboxyl ester lipase knockout mice.

    abstract::Carboxyl ester lipase (CEL; EC 3.1.1.13) hydrolyzes cholesteryl esters and retinyl esters in vitro. In vivo, pancreatic CEL is thought to liberate cholesterol and retinol from their esters prior to absorption in the intestine. CEL is also a major lipase in the breast milk of many mammals, including humans and mice, an...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi981679a

    authors: Weng W,Li L,van Bennekum AM,Potter SH,Harrison EH,Blaner WS,Breslow JL,Fisher EA

    更新日期:1999-03-30 00:00:00

  • Perturbation of the carboxy terminus of HIV-1 Rev affects multimerization on the Rev responsive element.

    abstract::Perturbations within the transactivation and carboxy-terminal domains of HIV-1 Rev were examined for effects on Rev responsive element (RRE) binding activities in vitro and biological activity in vivo. Binding affinities, specificities, and multimerization of the transactivation mutants M10 and Rev/Rex M10-16 on the R...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00085a028

    authors: Daly TJ,Rennert P,Lynch P,Barry JK,Dundas M,Rusche JR,Doten RC,Auer M,Farrington GK

    更新日期:1993-08-31 00:00:00

  • Biosynthesis of lipid A in Escherichia coli: identification of UDP-3-O-[(R)-3-hydroxymyristoyl]-alpha-D-glucosamine as a precursor of UDP-N2,O3-bis[(R)-3-hydroxymyristoyl]-alpha-D-glucosamine.

    abstract::The lipid A disaccharide of the Escherichia coli envelope is synthesized from the two fatty acylated glucosamine derivatives UDP-N2,O3-bis[(R)-3-hydroxytetradecanoyl]-alpha-D- glucosamine (UDP-2,3-diacyl-GlcN) and N2,O3-bis[(R)-3-hydroxytetradecanoyl]-alpha-D-glucosamine 1-phosphate (2,3-diacyl-GlcN-1-P) [Ray, B. L., ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00406a017

    authors: Anderson MS,Robertson AD,Macher I,Raetz CR

    更新日期:1988-03-22 00:00:00

  • Role of GTP hydrolysis in microtubule polymerization: evidence for a coupled hydrolysis mechanism.

    abstract::The relationship between GTP hydrolysis and microtubule assembly has been investigated by using a rapid filtration method. Microtubules assembled from phosphocellulose-purified tubulin, double-labeled with [gamma-32P]- and [3H]GTP, were trapped and washed free of unbound nucleotide on glass fiber filters. The transien...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00479a022

    authors: Stewart RJ,Farrell KW,Wilson L

    更新日期:1990-07-10 00:00:00

  • O2 activation by non-heme diiron proteins: identification of a symmetric mu-1,2-peroxide in a mutant of ribonucleotide reductase.

    abstract::Non-heme diiron clusters occur in a number of enzymes (e.g., ribonucleotide reductase, methane monooxygenase, and Delta9-stearoyl-ACP desaturase) that activate O2 for chemically difficult oxidation reactions. In each case, a kinetically labile peroxo intermediate is believed to form when O2 reacts with the diferrous e...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi981838q

    authors: Moënne-Loccoz P,Baldwin J,Ley BA,Loehr TM,Bollinger JM Jr

    更新日期:1998-10-20 00:00:00