The 0.78 A structure of a serine protease: Bacillus lentus subtilisin.

abstract：:Fructose-1,6-bisphosphate muscle aldolase is an essential glycolytic enzyme that catalyzes reversible carbon-carbon bond formation by cleaving fructose 1,6-bisphosphate to yield dihydroxyacetone phosphate (DHAP) and d-glyceraldehyde phosphate. To elucidate the mechanistic role of conserved amino acid Asp-33, Asn-33 an...

journal_title：Biochemistry

authors： St-Jean M,Blonski C,Sygusch J

更新日期：2009-06-02 00:00:00

abstract：:Several diseases, such as cancer, are characterized by acidification of the extracellular environment. Acidosis can be employed as a target to specifically direct therapies to the diseased tissue. We have used first principles to design an acidity-triggered rational membrane (ATRAM) peptide with high solubility in sol...

journal_title：Biochemistry

authors： Nguyen VP,Alves DS,Scott HL,Davis FL,Barrera FN

更新日期：2015-11-03 00:00:00

abstract：:It has previously been shown that 9S, untransformed progestin, estrogen, androgen, and glucocorticoid receptor complexes in rabbit uterine and liver cytosols contain a 59-kDa protein [Tai, P. K., Maeda, Y., Nakao, K., Wakim, N. G., Duhring, J. L., & Faber, L. E. (1986) Biochemistry 25, 5269-5275]. In this work we show...

journal_title：Biochemistry

authors： Sanchez ER,Faber LE,Henzel WJ,Pratt WB

更新日期：1990-05-29 00:00:00

abstract：:Cholesterol and its hemisuccinate and sulfate derivatives are widely used in studies of purified membrane proteins but are difficult to solubilize in aqueous solution, even in the presence of detergent micelles. Other cholesterol derivatives do not form conventional micelles and lead to viscous solutions. To address t...

journal_title：Biochemistry

authors： Howell SC,Mittal R,Huang L,Travis B,Breyer RM,Sanders CR

更新日期：2010-11-09 00:00:00

abstract：:The electron spin resonance probes 5-doxylstearate and 4-(dodecyldimethylammonio)-1-oxy-2,2,6,6-tetramethylpiperidine bromide were used to characterize the fluidity of the acyl chain and head-group regions, respectively, of defined salts of lipopolysaccharide (LPS) from Escherichia coli K12. The removal of the weakly ...

journal_title：Biochemistry

authors： Coughlin RT,Haug A,McGroarty EJ

更新日期：1983-04-12 00:00:00

abstract：:p-Hydroxyphenylpyruvate dioxygenase (HPD) plays a key role in the normal catabolism of tyrosine. An Fe2+/oxygen-dependent enzyme, it converts p-hydroxyphenylpyruvate into homogentisate and is part of the superfamily of alpha-ketoglutarate-dependent enzymes that couples oxidative decarboxylation of an alpha-ketoacid co...

journal_title：Biochemistry

authors： Gunsior M,Ravel J,Challis GL,Townsend CA

更新日期：2004-01-27 00:00:00

abstract：:Static FTIR light-induced difference spectra have been recorded for reaction centers from Rhodopseudomonas viridis in the following charge-separated states: P+QA(-)-PQA, P+QB(-)-PQB, I(-)-I, I-QA(-)-IQA, and I-QA(2-)-IQA. A comparison of the I(-)-I difference spectra with the I-QA(-)-IQA difference spectra reveals new...

journal_title：Biochemistry

authors： Buchanan S,Michel H,Gerwert K

更新日期：1992-02-11 00:00:00

abstract：:The properties of neuronal nitric oxide synthase containing one tetrahydrobiopterin (BH4) per dimer [nNOS(BH4+)] were compared to those of the BH4-free enzyme [nNOS(BH4-)]. The stimulation by BH4 of the formation of L-citrulline at the expense of H2O2 production unambiguously demonstrated that BH4 is essential in coup...

journal_title：Biochemistry

authors： Gorren AC,List BM,Schrammel A,Pitters E,Hemmens B,Werner ER,Schmidt K,Mayer B

更新日期：1996-12-24 00:00:00

abstract：:The chicken and goose egg white lysozymes (ChEWL and GoEWL) are homologues, but differ in substrate specificity. ChEWL catalyzes the hydrolysis of the glycosidic bonds of bacterial peptidoglycans and chitin-derived substrates, while GoEWL is specific for bacterial peptidoglycans. The active-site aspartate 52 residue o...

journal_title：Biochemistry

authors： Matsumura I,Kirsch JF

更新日期：1996-02-13 00:00:00

abstract：:Membrane-bound guanylate cyclases harbor a region called the dimerization or linker domain, which aids the enzymes in adopting an optimal monomer-monomer arrangement for catalysis. One subgroup of these guanylate cyclases is expressed in rod and cone cells of vertebrate retina, and mutations in the dimerization domain...

journal_title：Biochemistry

authors： Zägel P,Dell'Orco D,Koch KW

更新日期：2013-07-30 00:00:00

abstract：:Lecithin retinol acyltransferase (LRAT) catalyzes the esterification of all-trans-retinol into all-trans-retinyl ester, an essential reaction in the vertebrate visual cycle. Since all-trans-retinyl esters are the substrates for the isomerization reaction that generates 11-cis-retinoids, this esterification reaction is...

journal_title：Biochemistry

authors： Bok D,Ruiz A,Yaron O,Jahng WJ,Ray A,Xue L,Rando RR

更新日期：2003-05-27 00:00:00

abstract：:The reactivity of a series of para-substituted phenolic compounds in the peroxidation catalyzed by chloroperoxidase was investigated, and the results were interpreted on the basis of the binding characteristics of the substrates to the active site of the enzyme. Marked selectivity effects are observed. These operate t...

journal_title：Biochemistry

authors： Casella L,Poli S,Gullotti M,Selvaggini C,Beringhelli T,Marchesini A

更新日期：1994-05-31 00:00:00

abstract：:Chemical modification of some trifunctional amino acid residues in toxins I, II, and III of the scorpion Androctonus australis Hector have been performed. The results indicate: (1) Reduction and methylation of one disulfide bridge destroy toxic activity of toxin II. (2) The only tryptophan residue of toxin II (positio...

journal_title：Biochemistry

authors： Habersetzer-Rochat C,Sampieri F

更新日期：1976-06-01 00:00:00

abstract：:The N-type voltage-gated Ca(2+) channel CaV2.2 is one of the important targets for pain management. ω-Conotoxins isolated from venoms of cone snails, which specifically inhibit CaV2.2, are promising scaffolds for novel analgesics. The inhibitory action of ω-conotoxins on CaV2.2 has been examined experimentally, but th...

journal_title：Biochemistry

authors： Chen R,Chung SH

更新日期：2013-05-28 00:00:00

abstract：:Intestinal fatty acid binding protein (IFABP) and liver FABP (LFABP), homologous proteins expressed at high levels in intestinal absorptive cells, employ markedly different mechanisms for the transfer of fatty acids (FAs) to acceptor membranes. Transfer from IFABP occurs during protein-membrane collisional interaction...

journal_title：Biochemistry

authors： Córsico B,Liou HL,Storch J

更新日期：2004-03-30 00:00:00

abstract：:The unfolded state and flexible linkers in the folded structure play essential roles in protein stability and folding and protein-protein interactions. Intrinsic to these roles is the fact that unfolded proteins and flexible linkers sample many different conformations. Polymer models may capture this and complement ex...

journal_title：Biochemistry

authors： Zhou HX

更新日期：2004-03-02 00:00:00

abstract：:PhnZ utilizes a mixed valence diiron(II/III) cofactor and O2 to oxidatively cleave the carbon-phosphorus bond of (R)-2-amino-1-hydroxyethylphosphonic acid to form glycine and orthophosphate. The active site residues Y24 and E27 are proposed to mediate induced-fit recognition of the substrate and access of O2 to one of...

journal_title：Biochemistry

authors： Gama SR,Lo BSY,Séguin J,Pallitsch K,Hammerschmidt F,Zechel DL

更新日期：2019-12-31 00:00:00

abstract：:Monomeric sarcosine oxidase (MSOX) binds the L-proline zwitterion (pKa = 10.6). The reactive substrate anion is generated by ionization of the ES complex (pKa = 8.0). Tyr317 was mutated to Phe to determine whether this step might involve proton transfer to an active site base. The mutation does not eliminate the ioniz...

journal_title：Biochemistry

authors： Zhao G,Jorns MS

更新日期：2005-12-27 00:00:00

abstract：:The Met16Phe mutant of the type 1 copper protein pseudoazurin (PACu), in which a phenyl ring is introduced close to the imidazole moiety of the His81 ligand, has been characterized. NMR studies indicate that the introduced phenyl ring is parallel to the imidazole group of His81. The mutation has a subtle effect on the...

journal_title：Biochemistry

authors： Yanagisawa S,Sato K,Kikuchi M,Kohzuma T,Dennison C

更新日期：2003-06-10 00:00:00

abstract：:Ferrochelatase is the terminal enzyme in the heme biosynthetic pathway. It catalyzes the insertion of ferrous iron into protoporphyrin IX to produce protoheme IX. The crystal structures of ferrochelatase from Saccharomyces cerevisiae in free form, in complex with Co(II), a substrate metal ion, and in complex with two ...

journal_title：Biochemistry

authors： Karlberg T,Lecerof D,Gora M,Silvegren G,Labbe-Bois R,Hansson M,Al-Karadaghi S

更新日期：2002-11-19 00:00:00

abstract：:Plants employ a variety of light, oxygen, voltage (LOV) domain photoreceptors to regulate diverse aspects of growth and development. The Zeitlupe (ZTL), Flavin-Kelch-Fbox-1 (FKF1), and LOV-Kelch-Protein-2 (LKP2) proteins dictate measurement of the day length, flowering time, and regulation of the circadian clock by bl...

journal_title：Biochemistry

authors： Pudasaini A,Zoltowski BD

更新日期：2013-10-08 00:00:00

abstract：:The RmFixL-RmFixJ oxygen signal transduction system ensures that a cascade of the Sinorhizobium meliloti nitrogen fixation genes is induced as the concentration of O2 drops below 50 microM in symbiotic nodules. Deoxy-RmFixL is a histidine protein kinase that catalyzes a phosphoryl transfer from ATP to the aspartate 54...

journal_title：Biochemistry

authors： Sousa EH,Gonzalez G,Gilles-Gonzalez MA

更新日期：2005-11-22 00:00:00

abstract：:Vibrational Raman spectroscopic experiments have been performed as a function of temperature on aqueous dispersions of synthetic DL-erythro-N-lignoceroylsphingosylphosphocholine [C(24):SPM], a racemic mixture of two highly asymmetric hydrocarbon chain length sphingomyelins. Raman spectral peak-height intensity ratios ...

journal_title：Biochemistry

authors： Levin IW,Thompson TE,Barenholz Y,Huang C

更新日期：1985-10-22 00:00:00

abstract：:Fourier-transform infrared spectroscopy has been applied to the study of lipid vesicle-supported two-dimensional crystals and noncrystalline preparations of beef heart cytochrome oxidase. At room temperature, no conformational differences are seen between the noncrystalline and crystalline proteins, whose conformation...

journal_title：Biochemistry

authors： Arrondo JL,Castresana J,Valpuesta JM,Goñi FM

更新日期：1994-09-27 00:00:00

abstract：:In addition to encoding the tertiary fold and stability, the primary sequence of a protein encodes the folding trajectory and kinetic barriers that determine the speed of folding. How these kinetic barriers are encoded is not well understood. Here, we use evolutionary sequence variation in the α-lytic protease (αLP) p...

journal_title：Biochemistry

authors： Nixon CF,Lim SA,Sailer ZR,Zheludev IN,Gee CL,Kelch BA,Harms MJ,Marqusee S

更新日期：2021-01-26 00:00:00

abstract：:Time-resolved resonance Raman (TR(3)) and time-resolved step-scan (TRS(2)) FTIR spectroscopies have been used to probe the structural dynamics at the heme b(3) proximal and distal sites after carbon monoxide photolysis from fully reduced CO-bound nitric oxide reductase. The Raman spectra of the transient species exhib...

journal_title：Biochemistry

authors： Pinakoulaki E,Varotsis C

更新日期：2003-12-23 00:00:00

abstract：:We have obtained a quantitative description of villin-nucleated actin polymerization in physiological salt by determining the concentrations of free villin (V), villin-actin monomer (VA), villin-actin dimer (VA2), and villin-actin oligomer (VAn). Over a range of actin-villin ratios from 0.1 to 20 we determined the con...

journal_title：Biochemistry

authors： Weber A,Northrop J,Bishop MF,Ferrone FA,Mooseker MS

更新日期：1987-05-05 00:00:00

abstract：:The inactivation of cytochrome P450 2B4 by aldehydes in a reconstituted enzyme system requires molecular oxygen and NADPH and is not prevented by the addition of catalase, superoxide dismutase, epoxide hydrolase, glutathione, or ascorbic acid. A strong correlation between loss of enzymatic activity and bleaching of th...

journal_title：Biochemistry

authors： Raner GM,Chiang EW,Vaz AD,Coon MJ

更新日期：1997-04-22 00:00:00

abstract：:Murine fibroblasts cultured in the presence of fibroblast growth factor-1 express relatively high levels of FR-1, a approximately 36 kDa protein related to the aldo-keto reductase superfamily [Donohue, P. J., Alberts, G. F., Hampton, B. S., Winkles, J. A. (1994) J. Biol. Chem. 269, 8604-8609]. While the crystal struct...

journal_title：Biochemistry

authors： Srivastava S,Harter TM,Chandra A,Bhatnagar A,Srivastava SK,Petrash JM

更新日期：1998-09-15 00:00:00

abstract：:The solution structure of the complex formed between an oligodeoxynucleotide containing a two-base bulge (5'-CCATCGTCTACCTTTGGTAGGATGG) and SCA-alpha2, a designed spirocyclic helical molecule, has been elucidated. SCA-alpha2, a close mimic of the metabolite, NCSi-gb, of the DNA bulge-specific enediyne antibiotic neoca...

journal_title：Biochemistry

authors： Zhang N,Lin Y,Xiao Z,Jones GB,Goldberg IH

更新日期：2007-04-24 00:00:00