Abstract:
:The reactivity of a series of para-substituted phenolic compounds in the peroxidation catalyzed by chloroperoxidase was investigated, and the results were interpreted on the basis of the binding characteristics of the substrates to the active site of the enzyme. Marked selectivity effects are observed. These operate through charge, preventing phenolic compounds carrying amino groups on the substituent chain to act as substrates for the enzyme, and through size, excluding potential substrates containing bulky substituents to the phenol nucleus. Also, chiral recognition is exhibited by chloroperoxidase in the oxidation of N-acetyltyrosine, where only the L isomer is oxidized. Kinetic measurements show that, in general, the efficiency of chloroperoxidase in the oxidation of phenols is lower than that of horseradish peroxidase. Paramagnetic NMR spectra and relaxation rate measurements of chloroperoxidase-phenol complexes are consistent with binding of the substrates close to the heme, in the distal pocket, with the phenol group pointing toward the iron atom. On the other hand, phenolic compounds which are not substrates for chloroperoxidase bind to the enzyme with a much different disposition, with the phenol group very distant from the iron and probably actually outside the active-site cavity.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Casella L,Poli S,Gullotti M,Selvaggini C,Beringhelli T,Marchesini Adoi
10.1021/bi00187a001subject
Has Abstractpub_date
1994-05-31 00:00:00pages
6377-86issue
21eissn
0006-2960issn
1520-4995journal_volume
33pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Integrin αIIbβ3, a transmembrane heterodimer, mediates platelet aggregation when it switches from an inactive to an active ligand-binding conformation following platelet stimulation. Central to regulating αIIbβ3 activity is the interaction between the αIIb and β3 extracellular stalks, which form a tight heterodimer in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00430
更新日期:2019-07-30 00:00:00
abstract::The kinetics of tert-butyl isocyanide binding to the heme protein horseradish peroxidase (HRP) at 22 degrees C was examined on all time scales, from minutes to picoseconds, in aqueous borate buffer at pH 9.08. Unlike myoglobin (Mb) or hemoglobin, HRP shows two bimolecular ligand binding processes. For comparison, bind...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9518149
更新日期:1996-02-06 00:00:00
abstract::Glutaraldehyde treatment of electroplax membrane preparations of Na,K-ATPase leads to irreversible changes in the enzymic behavior of the protein, which are not due to modification of the active site. When the glutaraldehyde treatment is carried out in a medium containing K+ and without Na+, the "K+-modified enzyme" s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00288a016
更新日期:1983-09-13 00:00:00
abstract::Magic angle sample spinning (MASS) 13C NMR spectra have been obtained of bovine rhodopsin regenerated with retinal prosthetic groups isotopically enriched with 13C at C-5 and C-14. In order to observe the 13C retinal chromophore resonances, it was necessary to employ low temperatures (-15-----35 degrees C) to restrict...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00380a018
更新日期:1987-03-24 00:00:00
abstract::A well-defined region of pancreatic and other secreted phospholipase A2 (PLA2), which we call the i-face, makes a molecular contact with the interface to facilitate and control the events and processivity of the interfacial catalytic turnover cycles. The structural features of the i-face and its allosteric relationshi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9925470
更新日期:2000-05-16 00:00:00
abstract::Exogenous mannosylphosphoryldolichol (Man-P-Dol) has previously been shown to stimulate UDP-GlcNAc:dolichyl phosphate N-acetylglucosamine 1-phosphate transferase (GPT1), the enzyme catalyzing the biosynthesis of N-acetylglucosaminylpyrophosphoryldolichol (GlcNAc-P-P-Dol). To define the structural specificity of the ma...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00200a036
更新日期:1994-08-30 00:00:00
abstract::Energy coupling factor (ECF) transporters take up micronutrients in Bacteria and Archaea. They consist of a membrane-embedded S-component that provides substrate specificity and a three-subunit ECF module that couples ATP hydrolysis to transport. The S-components ThiT (for thiamin) and NiaX (for niacin) from Lactococc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00609
更新日期:2015-08-11 00:00:00
abstract::The interaction of serine protease (esterases) with 6-chloro-2-pyrones was investigated. Time-dependent inactivation of chymotrypsin, alpha-lytic protease, pig liver elastase, and cholinesterase was found with 3- and 5-benzyl-6-chloro-2-pyrone, as well as 3- and 5-methyl-6-chloro-2-pyrone. No inactivation was observed...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00282a034
更新日期:1983-06-21 00:00:00
abstract::Interaction of a mitochondria-specific anionic phospholipid, cardiolipin (CL), with an intermembrane protein, cytochrome c (cyt c), yields a peroxidase complex. During apoptosis, the complex induces accumulation of CL oxidation products that are essential for detachment of cyt c from the mitochondrial membrane, induct...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801507s
更新日期:2008-12-23 00:00:00
abstract::Introduction of a disulfide bond by site-directed mutagenesis was found to enhance the stability of subtilisin BPN' (EC 3.4.21.14) under a variety of conditions. The location of the new disulfide bond was selected with the aid of a computer program, which scored various sites according to the amount of distortion that...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00382a002
更新日期:1987-04-21 00:00:00
abstract::Living cells contain a range of densely phosphorylated surfaces, including phospholipid membranes, ribonucleoproteins, and nucleic acid polymers. Hyperphosphorylated surfaces also accumulate in neurodegenerative diseases as neurofibrillar tangles. We have synthesized and structurally characterized a precisely patterne...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500599a
更新日期:2014-07-08 00:00:00
abstract::A mouse monoclonal anti-7-(O-carboxymethyl)oximinoestradiol antibody was photoaffinity labeled with two cross-reactive 6alpha- and 6beta-(5-azido-2-nitrobenzoyl)amido[17alpha-3H]estradiol photoreagents (6alpha- and 6beta-ANBA-[17alpha-3H]estradiol). Covalently bound radioactivity was found exclusively on the light cha...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962645a
更新日期:1997-06-24 00:00:00
abstract::Heparin potentiates the mitogenic activity of FGF-1 by increasing the affinity for its receptor and by extending its biological half-life. During the course of labeling human FGF-1 with Na(125)I and chloramine T, it was observed that the protein lost its ability to bind to heparin. In contrast, bovine FGF-1 retained i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9903345
更新日期:1999-07-20 00:00:00
abstract::Nonsense mutations that result in premature stop codons in the HBB gene cause β-thalassemia. This disease is characterized by a reduced hemoglobin level due to the lack of β-globin. Compounds that induce translational readthrough across the thalassemia-causing premature stop codon will have therapeutic benefits. Curre...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00761
更新日期:2020-01-14 00:00:00
abstract::In an effort to gain greater insight into the molecular mechanism of the electron-transfer reactions of cytochrome b(5), the bovine cytochrome b(5)-horse cytochrome c complex has been investigated by high-resolution multidimensional NMR spectroscopy using (13)C, (15)N-labeled cytochrome b(5) expressed from a synthetic...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001129o
更新日期:2000-11-21 00:00:00
abstract::The Escherichia coli L-arabinose-binding protein (ABP) participates as a specific receptor in the transport of L-arabinose, D-fucose, and D-galactose through the periplasmic space. The wild-type protein binds L-arabinose about 40 times more strongly than D-fucose. A mutation of the protein at position 108 (Met-->Leu) ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00080a013
更新日期:1993-07-27 00:00:00
abstract::Double-stranded complementary deoxyribonucleic acid (cDNA) transcribed in vitro from a partially pure myosin light-chain messenger ribonucleic acid (mRNA) of the chick embryonic heart was cloned in Escherichia coli strain chi 1776 by using the HindIII cleavage site in the plasmid pBR322. The insertion of essentially f...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00592a019
更新日期:1979-12-11 00:00:00
abstract::The kinetics of formation of the ADP-sensitive (EP) and ADP-insensitive (E*P) phosphoenzyme intermediates of the CaATPase in sarcoplasmic reticulum (SR) were investigated by means of the quenched-flow technique. At 21 degrees C, addition of saturating ADP to SR vesicles phosphorylated for 116 ms with 10 microM ATP gav...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00322a018
更新日期:1985-01-01 00:00:00
abstract::The binding site of cyclosporin A to P-glycoprotein was characterized by using a multidrug-resistant Chinese hamster ovary cell line. P-glycoprotein photolabeled with diazirine-cyclosporin A analogue was purified by a two-step process involving continuous elution electrophoresis followed by wheat germ agglutinin-agaro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981992c
更新日期:1998-12-22 00:00:00
abstract::Immunological methods, in parellel with measurement of ATPase activity, have been used to characterize the reactions of antibodies specific for light chains with myosin and its water-soluble proteolytic subfragments, heavy meromyosin (HMM) and subfragment 1 (HMM S-1). Antiserum to the 5,5'-dithiobis(2-nitro-enzoic aci...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00692a008
更新日期:1975-10-21 00:00:00
abstract::Monomolecular layers of undissociated bile acids and membrane lipids at the air/water interface serve as useful models for the interactions between fully ionized bile salts and physiological membranes. Employing an automated Langmuir-Pockels surface balance, surface pressures and dipole moments were measured as functi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00034a023
更新日期:1995-08-29 00:00:00
abstract::The membrane stabilization effect of cholesteryl hemisuccinate (CHEMS) and the sensitivity of the CHEMS-phosphatidylethanolamine membranes to protons and calcium ions were studied by differential scanning calorimetry, freeze-fracture electron microscopy, and 31P NMR. (1) At neutral pH, the addition of 8 mol % CHEMS to...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00328a013
更新日期:1985-03-26 00:00:00
abstract::Most of the molybdoenzymes contain, in the amino-terminal region of their catalytic subunits, a conserved Cys group that in some cases binds an [Fe-S] cluster. In dissimilatory nitrate reductases, the first Cys residue of this motif is replaced by a conserved His residue. Site-directed mutagenesis of this residue (His...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972858f
更新日期:1998-05-19 00:00:00
abstract::Cytochrome P450 (P450) 3A4 is the most abundant human P450 and oxidizes a diversity of substrates, including various drugs, steroids, carcinogens, and macrolide natural products. In some reactions, positive cooperativity has been reported in microsomal studies. Flavonoids, e.g., 7,8-benzoflavone (alpha-naphthoflavone,...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962359z
更新日期:1997-01-14 00:00:00
abstract::The sequence of two overlapping cDNA clones for the zinc metalloproteinase hemorrhagic toxin e (also known as atrolysin e, EC 3.4.24.44) from the venom gland of Crotalus atrox, the Western diamondback rattlesnake, is presented. The assembled cDNA sequence is 1975 nucleotides in length and encodes an open reading frame...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00142a005
更新日期:1992-07-14 00:00:00
abstract::The mechanism of codon recognition by tRNA is investigated in the system tRNAPhe + UUC by temperature-jump measurements using the Wye base fluorescence as a label. In 0.4 M Na+ and 5 mM Mg2+ a two-step reaction is observed and described quantitatively; UUC is shown to bind preferentially to one of two conformations on...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00557a023
更新日期:1980-08-05 00:00:00
abstract::Rhodopsin kinase phosphorylates serine- and threonine-containing peptides from bovine rhodopsin's carboxyl-terminal sequence. Km's for the peptides decrease as the length of the peptide is increased over the range 12-31 amino acids, reaching 1.7 mM for peptide 318-348 from the rhodopsin sequence. The Km for phosphoryl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00448a013
更新日期:1989-10-31 00:00:00
abstract::The retaining beta-1,4-glycanase Cex from Cellulomonas fimi, a family 10 glycosyl hydrolase, hydrolyzes xylan 40-fold more efficiently than cellulose. To gain insight into the nature of its preference for xylan, we determined the crystal structure of the Cex catalytic domain (Cex-cd) trapped as its covalent 2-deoxy-2-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9729211
更新日期:1998-04-07 00:00:00
abstract::The Vmax/Km (microM -1 min -1.) for bovine factor X activation by bovine factor IXa alpha, in the presence of sufficient [Ca2+] to saturate the initial reaction rate, was 0.007. When factor IXa beta was substituted for factor IXa alpha in this reaction, the Vmax/Km decreased to 0.001, suggesting that factor IXa alpha ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00286a007
更新日期:1983-08-16 00:00:00
abstract::The structure of the 56-residue B1 immunoglobulin-binding domain from streptococcal protein G has been determined in two different crystal forms. The crystal structures were deduced by molecular replacement, based on the structure of the B2 domain (Brookhaven accession code 1PGX). Final R values are 0.174 and 0.198 fo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:
更新日期:1994-04-19 00:00:00