Specific photoaffinity labeling of Tyr-49 on the light chain in the steroid-combining site of a mouse monoclonal anti-estradiol antibody using two epimeric 6alpha- and 6beta-(5-azido-2-nitrobenzoyl)amidoestradiol photoreagents.

abstract：:Sequential 1H NMR assignments of a metallothionein from Neurospora crassa have been accomplished by the combined use of COSY, 2QF-COSY, HOHAHA, and rotating-frame NOE experiments. All potentially observable resonances were assigned except for the epsilon-NH3 group of the C-terminal lysine. 1H NOEs, when observed in th...

journal_title：Biochemistry

authors： Malikayil JA,Lerch K,Armitage IM

更新日期：1989-04-04 00:00:00

abstract：:The kinetics of reduction of free flavin semiquinones of the individual components of 1:1 covalent and electrostatic complexes of yeast ferric and ferryl cytochrome c peroxidase and ferric horse cytochrome c have been studied. Covalent cross-linking between the peroxidase and cytochrome c at low ionic strength results...

journal_title：Biochemistry

authors： Hazzard JT,Moench SJ,Erman JE,Satterlee JD,Tollin G

更新日期：1988-03-22 00:00:00

abstract：:In vitro transcription by T7 RNA polymerase was used to prepare 32 different mutations in the 21 nucleotides that participate in the 9 tertiary base pairs or triples of yeast tRNAPhe. The mutations were designed either to disrupt the tertiary interaction or to change the sequence without disrupting the structure by tr...

journal_title：Biochemistry

authors： Sampson JR,DiRenzo AB,Behlen LS,Uhlenbeck OC

更新日期：1990-03-13 00:00:00

abstract：:The BARD1 N-terminal RING domain binds BRCA1 while the BARD1 C-terminal ankyrin and tandem BRCT repeat domains bind CstF-50 to modulate mRNA processing and RNAP II stability in response to DNA damage. Here we characterize the BARD1 structural biochemistry responsible for CstF-50 binding. The crystal structure of the B...

journal_title：Biochemistry

authors： Edwards RA,Lee MS,Tsutakawa SE,Williams RS,Nazeer I,Kleiman FE,Tainer JA,Glover JN

更新日期：2008-11-04 00:00:00

abstract：:The acetylenic analogue of adenosine 9-(5',6'-dideoxy-beta-D-ribo-hex-5'-ynofuranosyl)adenine has been synthesized, and its behavior as an inhibitor of bovine S-adenosylhomocysteine hydrolase has been examined. Incubation of the enzyme with excess inhibitor caused a time-dependent, irreversible inactivation of the enz...

journal_title：Biochemistry

authors： Parry RJ,Muscate A,Askonas LJ

更新日期：1991-10-15 00:00:00

abstract：:We examine the relationship between binding affinity and interface size for reversible protein-protein interactions (PPIs), using cytokines from the tumor necrosis factor (TNF) superfamily and their receptors as a test case. Using surface plasmon resonance, we measured single-site binding affinities for binding of the...

journal_title：Biochemistry

authors： Day ES,Cote SM,Whitty A

更新日期：2012-11-13 00:00:00

abstract：:The divalent cation requirement for partial reactions of the catalytic turnover cycle during interfacial catalysis by pig pancreatic phospholipase A2 (PLA2) is investigated. Results show that the specific role of calcium in all the events of the catalytic cycle at the active site is not shared by other divalent cation...

journal_title：Biochemistry

authors： Yu BZ,Berg OG,Jain MK

更新日期：1993-06-29 00:00:00

abstract：:The binding of bleomycin to DNA in the presence and absence of ferric iron was measured by fluorescence spectroscopy. In millimolar concentrations of tris(hydroxymethyl)aminomethane, pH 7.5, approximately 80% of the bleomycin binds to DNA. Ferric iron seems to have no significant effect on the binding of DNA to bleomy...

journal_title：Biochemistry

authors： Caspary WJ,Lanzo DA,Niziak C

更新日期：1982-01-19 00:00:00

abstract：:The mechanism of the oxidative decarboxylation reaction catalyzed by the NAD-malic enzyme from Ascaris suum has been examined with several different divalent metal ion activators and dinucleotide substrates. Primary deuterium and tritium isotope effects have been obtained and, in combination with the partitioning rati...

journal_title：Biochemistry

authors： Karsten WE,Gavva SR,Park SH,Cook PF

更新日期：1995-03-14 00:00:00

abstract：:The Escherichia coli siderophore enterobactin is assembled from 2,3-dihydroxybenzoate (2,3-DHB) and l-serine by the nonribosomal peptide synthetases EntB and EntF. The processive thiol-template strategy used can be sabotaged by EntB misacylation. Through in vitro kinetic analysis, we demonstrate two potential routes t...

journal_title：Biochemistry

authors： Chen D,Wu R,Bryan TL,Dunaway-Mariano D

更新日期：2009-01-27 00:00:00

abstract：:The intestinal fatty acid binding protein (IFABP) is a small (15 kDa) protein consisting mostly of 10 antiparallel beta-strands (A-J) and a small helical region that serves as a portal for the ligand. Two beta-sheet structures (strands A-E and F-J) surround a cavity into which the ligand binds. In this work, we invest...

journal_title：Biochemistry

authors： Rajabzadeh M,Kao J,Frieden C

更新日期：2003-10-28 00:00:00

abstract：:Human type-alpha transforming growth factor (hTGF alpha) is a small mitogenic protein containing 50 amino acids and three disulfide bonds. It has both sequence and structural homology with epidermal growth factor (EGF). While the three-dimensional structures of hTGF alpha and other EGF-like proteins have been studied ...

journal_title：Biochemistry

authors： Li YC,Montelione GT

更新日期：1995-02-28 00:00:00

abstract：:Acetohydroxy acid synthase (AHAS) is a thiamin diphosphate (ThDP)-dependent enzyme that catalyzes the first common step in the biosynthesis of branched-chain amino acids, condensation of pyruvate with a second 2-ketoacid to form either acetolactate or acetohydroxybutyrate. AHAS isozyme II from Escherichia coli is spec...

journal_title：Biochemistry

authors： Vyazmensky M,Steinmetz A,Meyer D,Golbik R,Barak Z,Tittmann K,Chipman DM

更新日期：2011-04-19 00:00:00

abstract：:We have undertaken ultrafast infrared (IR) spectroscopic studies in order to elucidate the geometry of bound CO in the alpha and beta subunits of hemoglobin (Hb) M Boston 13CO. Hb M Boston is a mutant human Hb in which the distal histidine in the alpha subunits is replaced by a tyrosine. The IR absorptions of bound 13...

journal_title：Biochemistry

authors： Lian T,Locke B,Kitagawa T,Nagai M,Hochstrasser RM

更新日期：1993-06-08 00:00:00

abstract：:Mitochondria isolated from coenzyme Q deficient yeast cells had no detectable NADH:cytochrome c reductase or succinate:cytochrome c reductase activity but contained normal amounts of cytochromes b and c1 by spectral analysis. Addition of the exogenous coenzyme Q derivatives including Q2, Q6, and the decyl analogue (DB...

journal_title：Biochemistry

authors： Beattie DS,Clejan L

更新日期：1986-03-25 00:00:00

abstract：:The structural underpinnings of enzyme substrate specificity are investigated in a pair of copper amine oxidases (CAOs) from Hansenula polymorpha (HPAO-1 and HPAO-2). The X-ray crystal structure (to 2.0 A resolution) and steady state kinetic data of the second copper amine oxidase (HPAO-2) are presented for comparison...

journal_title：Biochemistry

authors： Chang CM,Klema VJ,Johnson BJ,Mure M,Klinman JP,Wilmot CM

更新日期：2010-03-23 00:00:00

abstract：:Engineering proteins to withstand a broad range of conditions continues to be a coveted objective, holding the potential to advance biomedicine, industry, and our understanding of disease. One way of achieving this goal lies in elucidating the underlying interactions that define protein stability. It has been shown th...

journal_title：Biochemistry

authors： Strickler SS,Gribenko AV,Gribenko AV,Keiffer TR,Tomlinson J,Reihle T,Loladze VV,Makhatadze GI

更新日期：2006-03-07 00:00:00

abstract：:Thermodynamic data for RNA 1 x 2 nucleotide internal loops are lacking. Thermodynamic data that are available for 1 x 2 loops, however, are for loops that rarely occur in nature. In order to identify the most frequently occurring 1 x 2 nucleotide internal loops, a database of 955 RNA secondary structures was compiled ...

journal_title：Biochemistry

authors： Badhwar J,Karri S,Cass CK,Wunderlich EL,Znosko BM

更新日期：2007-12-18 00:00:00

abstract：:Anthrax toxin receptor 1 (ANTXR1)/tumor endothelial marker 8 (TEM8) is one of two known proteinaceous cell surface anthrax toxin receptors. A metal ion dependent adhesion site (MIDAS) present in the integrin-like inserted (I) domain of ANTXR1 mediates the binding of the anthrax toxin subunit, protective antigen (PA). ...

journal_title：Biochemistry

authors： Ramey JD,Villareal VA,Ng C,Ward SC,Xiong JP,Clubb RT,Bradley KA

更新日期：2010-08-31 00:00:00

abstract：:Biochemical and immunological properties of biosynthetically radiolabeled phosphatidylcholine-(PC-) binding proteins were investigated. The PC-binding proteins were extracted from the detergent lysate of biosynthetically radiolabeled P388D1 cells by affinity chromatography on PC-Sepharose and filtered through a Sephad...

journal_title：Biochemistry

authors： Fernandez-Botran R,Suzuki T

更新日期：1985-04-09 00:00:00

abstract：:The class C serine beta-lactamase of Enterobacter cloacae P99 was inhibited by a series of aryl methylphosphonate monoester monoanions. The effectiveness of these inhibitors was promoted by an acylamido substituent on the methyl group and a good leaving group at phosphorus. The former preference suggests that noncoval...

journal_title：Biochemistry

authors： Rahil J,Pratt RF

更新日期：1992-06-30 00:00:00

abstract：:Sf9 cells infected with a recombinant baculovirus containing the gene for human prorenin were cultured in the presence of [3H]mannose. In vivo labeled prorenin was isolated by immunoprecipitation from the culture medium and digested with Pronase. The oligosaccharide structures on the resulting glycopeptides were analy...

journal_title：Biochemistry

authors： Aeed PA,Elhammer AP

更新日期：1994-07-26 00:00:00

abstract：:The technique of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) has been applied to a mesophilic (E. coli) dihydrofolate reductase under conditions that allow direct comparison to a thermophilic (B. stearothermophilus) ortholog, Ec-DHFR and Bs-DHFR, respectively. The analysis of hydrogen-deuterium e...

journal_title：Biochemistry

authors： Oyeyemi OA,Sours KM,Lee T,Kohen A,Resing KA,Ahn NG,Klinman JP

更新日期：2011-09-27 00:00:00

abstract：:Thioacetamide, a hepatocarcinogen and an inhibitor of heme synthesis, blocks the phenobarbitone-mediated increase in the transcription of cytochrome P-450b+e messenger RNA in rat liver. This property is also shared by CoCl2 and 3-amino-1,2,4-triazole, two other inhibitors of heme synthesis. Thus, it appears feasible t...

journal_title：Biochemistry

authors： Satyabhama S,Seelan RS,Padmanaban G

更新日期：1986-08-12 00:00:00

abstract：:The left-handed polyproline II (P(II)) helix is a structure that has been given a great deal of attention lately because of its role in a wide variety of physiologically important processes and potential significance in protein unfolded states. Recent work by several authors has shown that residues besides proline can...

journal_title：Biochemistry

authors： Chellgren BW,Creamer TP

更新日期：2004-05-18 00:00:00

abstract：:Further to our studies on the Escherichia coli 30S ribosomal subunit, the detailed surface topography of both 50S subunits and 70S ribosomes has been investigated by using iodination catalyzed by immobilized lactoperoxidase as the surface probe. In the 50S subunit, only proteins L2, L5, L10, and L11 were iodinated to ...

journal_title：Biochemistry

authors： Maly P,Wower J,Zobawa M,Brimacombe R

更新日期：1983-06-21 00:00:00

abstract：:Urease from Klebsiella aerogenes [Jabri et al. (1995) Science 268, 998-1004] is an (alpha beta gamma)3 trimer with each alpha-subunit having an (alpha beta)8-barrel domain containing a binickel active center. Here we examine structure-function relations for urease in more detail through structural analysis of the urea...

journal_title：Biochemistry

authors： Jabri E,Karplus PA

更新日期：1996-08-20 00:00:00

abstract：:Retinal rod and cone cGMP phosphodiesterases (PDE6 family) function as the effector enzyme in the vertebrate visual transduction cascade. The activity of PDE6 catalytic subunits is controlled by the Pgamma-subunits. In addition to the inhibition of cGMP hydrolysis at the catalytic sites, Pgamma is known to stimulate a...

journal_title：Biochemistry

authors： Muradov KG,Granovsky AE,Schey KL,Artemyev NO

更新日期：2002-03-26 00:00:00

abstract：:The side-chain dynamics of methyl groups in two structurally related proteins from the fibronectin type III (fnIII) superfamily, the third fnIII domain from human tenascin (TNfn3) and the tenth fnIII domain from human fibronectin (FNfn10), have been studied by NMR spectroscopy. Side-chain order parameters reveal that ...

journal_title：Biochemistry

authors： Best RB,Rutherford TJ,Freund SM,Clarke J

更新日期：2004-02-10 00:00:00

abstract：:We determined the magnitude of the bend induced in DNA by an adenine-thymine tract by measuring the rate of cyclization of DNA oligonucleotides containing phased A tracts. A series of linear multimers with 2-bp single-stranded ends, in which the (A.T)6 tracts are separated by CG2-3C sequences and are positioned 10 and...

journal_title：Biochemistry

authors： Koo HS,Drak J,Rice JA,Crothers DM

更新日期：1990-05-01 00:00:00