Abstract:
:Heparin potentiates the mitogenic activity of FGF-1 by increasing the affinity for its receptor and by extending its biological half-life. During the course of labeling human FGF-1 with Na(125)I and chloramine T, it was observed that the protein lost its ability to bind to heparin. In contrast, bovine FGF-1 retained its heparin affinity even after iodination. To localize the region responsible for the lost heparin affinity, chimeric FGF-1 proteins were constructed from human and bovine FGF-1 expression constructs and tested for their heparin affinity after iodination. The results showed that the C-terminal region of human FGF-1 was responsible for the loss of heparin affinity. This region harbors a single tyrosine residue in human FGF-1 in contrast to a phenylalanine at this position in bovine FGF-1. Mutating this tyrosine residue in the human FGF-1 sequence to phenylalanine did not restore the heparin affinity of the iodinated protein. Likewise, changing the phenylalanine to tyrosine in the bovine FGF-1 did not reduce the ability of the iodinated protein to bind to heparin. In contrast, a mutant human FGF-1 that has cysteine-131 replaced with serine (C131S) was able to bind to heparin even after iodination while bovine FGF-1 (S131C) lost its binding affinity to heparin upon iodination. In addition, the human FGF-1 C131S mutant showed a decrease in homodimer formation when exposed to CuCl(2). Molecular modeling showed that the heparin-binding domain of FGF-1 includes cysteine-131 and that cysteine-131, upon oxidation to cysteic acid during the iodination procedures, would interact with lysine-126 and lysine-132. This interaction alters the conformation of the basic residues such that they no longer bind to heparin.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Patrie KM,Botelho MJ,Franklin K,Chiu IMdoi
10.1021/bi9903345subject
Has Abstractpub_date
1999-07-20 00:00:00pages
9264-72issue
29eissn
0006-2960issn
1520-4995pii
bi9903345journal_volume
38pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::GlgE (EC 2.4.99.16) is an α-maltose 1-phosphate:(1→4)-α-d-glucan 4-α-d-maltosyltransferase of the CAZy glycoside hydrolase 13_3 family. It is the defining enzyme of a bacterial α-glucan biosynthetic pathway and is a genetically validated anti-tuberculosis target. It catalyzes the α-retaining transfer of maltosyl units...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500183c
更新日期:2014-04-22 00:00:00
abstract::Serotonergic signaling appears to play a key role in the generation and modulation of various cognitive, behavioral, and developmental processes. The serotonin(1A) receptor is an important member of the superfamily of seven transmembrane domain G-protein-coupled receptors and is the most extensively studied among the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0480887
更新日期:2004-12-21 00:00:00
abstract::Sequential 1H NMR assignments of a metallothionein from Neurospora crassa have been accomplished by the combined use of COSY, 2QF-COSY, HOHAHA, and rotating-frame NOE experiments. All potentially observable resonances were assigned except for the epsilon-NH3 group of the C-terminal lysine. 1H NOEs, when observed in th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00433a038
更新日期:1989-04-04 00:00:00
abstract::Lipoprotein lipase (LPL) is a dimeric enzyme that is responsible for clearing triglyceride-rich lipoproteins from the blood. Although LPL plays a key role in cardiovascular health, an experimentally derived three-dimensional structure has not been determined. Such a structure would aid in understanding mutations in LP...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b01009
更新日期:2018-01-16 00:00:00
abstract::CHO cells expressing the human insulin receptors (IR) were used to evaluate the effect of the potent farnesyltransferase inhibitor, manumycin, on insulin antiapoptotic function. Cell treatment with manumycin blocked insulin's ability to suppress pro-apoptotic caspase-3 activity which led to time-dependent proteolytic ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000995y
更新日期:2000-10-17 00:00:00
abstract::Ultraviolet resonance Raman (RR) spectra, with 200- and 218-nm excitation from a H2-shifted quadrupled Nd:YAG laser, are reported for insulin and alpha-lactalbumin in dilute aqueous solution, at pH values known to produce differences in the exposure of the aromatic residues to solvent. At 200 nm, the spectra are domin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00329a009
更新日期:1985-04-09 00:00:00
abstract::Water deuteron NMR spectra have been studied for the system dipalmitoyllecithin (DPL)-heavy water (D2O) at different compositions and temperatures. From an analysis of the spectra in terms of quadrupole splittings, a phase diagram has been constructed for the temperature range 25-60 degrees C and the composition range...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00645a014
更新日期:1977-12-27 00:00:00
abstract::Recent crystallographic data suggest that conserved residues in the connection subdomain and C-terminal ribonuclease H (RNase H) domain of human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT) contact the nascent DNA primer and modulate the trajectory of the template relative to the RNase H catalytic ce...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi015970t
更新日期:2002-04-16 00:00:00
abstract::The amino acid sequence of human alpha-fetoprotein, a 67-kDa protein present in mammalian embryonic serum, was verified by fast atom bombardment mass spectrometric (FAB/MS) analyses of three different enzymatic digests of the protein. Human alpha-fetoprotein obtained from a large-scale cell culture was digested with t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00234a032
更新日期:1991-05-21 00:00:00
abstract::Guanidine hydrochloride (GdnHCl) and thermally induced unfolding measurements on the oxidized form of Escherichia coli thioredoxin at pH 7 were combined for the purpose of assessing the functional dependence of unfolding free energy changes on denaturant concentration over an extended GdnHCl concentration range. Conve...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00135a022
更新日期:1992-05-26 00:00:00
abstract::A quartz-crystal microbalance (QCM) technique was applied to analyze effects of site-directed mutagenesis of a glycosidase (isomalto-dextranase) on the hydrolysis mechanism of the substrate binding (k(on), k(off), and K(d)) and the catalytic process (k(cat)), separately, by using a dextran-immobilized QCM in buffer so...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi050079q
更新日期:2005-07-12 00:00:00
abstract::A preparation consisting of isolated dimeric peripheral antenna complexes from green plant photosystem I (light-harvesting complex I or LHCI) has been characterized by means of (polarized) steady-state absorption and fluorescence spectroscopy at low temperatures. We show that this preparation can be described reasonab...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0007369
更新日期:2000-07-25 00:00:00
abstract::The Escherichia coli repressor of biotin biosynthesis is both a biotin ligase and the repressor of transcriptional initiation at the biotin biosynthetic operon. The small molecule, biotinyl-5'-adenylate (bio-5'-AMP), is the intermediate in the biotin ligation reaction and the positive allosteric effector for sequence-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00189a041
更新日期:1994-06-14 00:00:00
abstract::Specificity of the binding of sterols and related compounds with purified F-protein (fusion protein) of the HVJ (Sendai virus) was studied by binding competition with [3H]cholesterol. Requirement for cholesterol or the A/B ring trans structure and nonrequirement for the 3-hydroxyl group were found in this binding. Bin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00404a035
更新日期:1988-02-23 00:00:00
abstract::Electrophilic halogenating agents, including hypohalous acids and haloamines, oxidize free methionine and the N-terminal methionines of peptides and proteins (e.g., Met-1 of anti-inflammatory peptide 1 and ubiquitin) to produce dehydromethionine (a five-membered isothiazolidinium heterocycle). Amide derivatives of met...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901343d
更新日期:2009-11-24 00:00:00
abstract::Phage display technique is a powerful tool with which to identify novel binding sequences for antibody and receptor targets. Few studies, however, have used this technology to select affinity peptides for ligand molecules. Here, we screened a peptide phage library for binding to toxic shock syndrome toxin 1 (TSST-1) t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi960132y
更新日期:1996-08-13 00:00:00
abstract::The substrate specificities of two incorrectly annotated enzymes belonging to cog3964 from the amidohydrolase superfamily were determined. This group of enzymes are currently misannotated as either dihydroorotases or adenine deaminases. Atu3266 from Agrobacterium tumefaciens C58 and Oant2987 from Ochrobactrum anthropi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi301483z
更新日期:2013-01-08 00:00:00
abstract::The spin-1/2-carrying NO molecule interacts with both the S1 and S2 states of the water oxidizing complex. The intermediates of the interaction can be resolved and trapped by NO treatment at subzero temperatures. At -30 degrees C and in the presence of approx. 500-700 microM NO, S1 loses the ability to yield by illumi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962899w
更新日期:1997-07-29 00:00:00
abstract::In experiments designed to characterize the basis of amyloid fibril stability through mutational analysis of the Abeta (1-40) molecule, fibrils exhibit consistent, significant structural malleability. In these results, and in other properties, amyloid fibrils appear to more resemble plastic materials generated from sy...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi0620959
更新日期:2007-01-09 00:00:00
abstract::The gene coding for the enzyme 2,3-dihydroxybenzoate-AMP ligase (2,3DHB-AMP ligase), responsible for activating 2,3-dihydroxybenzoic acid in the biosynthesis of the siderophore enterobactin, has been subcloned into the multicopy plasmid pKK223-3 and overproduced in a strain of Escherichia coli. The protein is an alpha...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00443a008
更新日期:1989-08-22 00:00:00
abstract::The recently isolated Escherichia coli murB gene (Pucci et al., 1992) has been cloned into an expression vector and the encoded UDP-N-acetylenolpyruvylglucosamine reductase (EC 1.1.1.158) was overproduced to about 10% of soluble cell protein. The encoded 38-kDa protein has been purified to near homogeneity. It was fou...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00059a019
更新日期:1993-03-02 00:00:00
abstract::Sulfhydryl-reactive derivatives of glucosamine were synthesized as potentially transportable affinity labels of the human erythrocyte hexose carrier. N-Maleoylglycyl derivatives of either 6- or 2-amino-2-deoxy-D-glucopyranose were the most potent inhibitors of 3-O-methylglucose uptake, with concentrations of half-maxi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00430a044
更新日期:1989-02-21 00:00:00
abstract::13C NMR spectroscopy has been used to study the amino group environments and metal binding properties of 13C reductively methylated bovine alpha-lactalbumin. Bovine alpha-lactalbumin is a Ca2+ metalloprotein containing 12 lysyl amino groups and a free amino terminus. All 13 amino groups can be 13C-dimethylated without...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00315a026
更新日期:1984-09-25 00:00:00
abstract::Toc34 is a transmembrane protein located in the outer envelope membrane of chloroplasts and involved in transit peptide recognition. The cytosolic region of Toc34 reveals 34% alpha-helical and 26% beta-strand structure and is stabilized by intramolecular electrostatic interaction. Toc34 binds both chloroplast preprote...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011361+
更新日期:2002-02-12 00:00:00
abstract::Porcine parathyroid gland slices were incubated with 3H-labeled amino acids in order to label tissue proteins. After incubation a crude hormonal extract was prepared and analyzed by chromatography on carboxymethylcellulose. Among the three radioactive peaks which were detected in the eluate, two were identified as par...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00687a018
更新日期:1975-08-12 00:00:00
abstract::The luminescence properties (fluorescence and phosphorescence) of luliberin have been investigated at liquid-nitrogen temperature (77 K) in 50% ethylene glycolaqueous buffer at various pH's. Calculation of the energy-transfer efficiency between Trp and Tyr residues leads to an evaluation of the distance separating the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00671a009
更新日期:1976-12-28 00:00:00
abstract::Vaccinia DNA topoisomerase, a 314 amino acid type I enzyme, catalyzes the cleavage and rejoining of DNA strands through a DNA-(3'-phosphotyrosyl)-enzyme intermediate formed at a specific target sequence, 5'-(C/T)CCTT downward arrow. To identify amino acids that participate in the DNA binding and transesterification st...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970498q
更新日期:1997-07-01 00:00:00
abstract::Monomolecular layers of undissociated bile acids and membrane lipids at the air/water interface serve as useful models for the interactions between fully ionized bile salts and physiological membranes. Employing an automated Langmuir-Pockels surface balance, surface pressures and dipole moments were measured as functi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00034a023
更新日期:1995-08-29 00:00:00
abstract::Elucidation of the molecular mechanism leading to biomembrane fusion is a challenging issue in current biomedical research in view of its involvement in controlling cellular functions and in mediating various important diseases. According to the generally admitted stalk mechanism described for membrane fusion, negativ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060317x
更新日期:2006-08-01 00:00:00
abstract::Glucocorticoid (GC) hormones induce apoptosis in T-cell and pre-B-cell acute lymphoblastic leukemia (ALL) cells. Steroid-mediated apoptosis requires a threshold level of the glucocorticoid receptor (GR) protein, and increasing the intracellular GR levels in ALL cells would augment their hormone sensitivity. A protein ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi1007723
更新日期:2010-10-19 00:00:00