Abstract：
:CHO cells expressing the human insulin receptors (IR) were used to evaluate the effect of the potent farnesyltransferase inhibitor, manumycin, on insulin antiapoptotic function. Cell treatment with manumycin blocked insulin's ability to suppress pro-apoptotic caspase-3 activity which led to time-dependent proteolytic cleavage of two nuclear target proteins. The Raf-1/MEK/ERK cascade and the serine/threonine protein kinase Akt are two survival pathways that may be activated in response to insulin. We tested the hypothesis that inhibition of farnesylated Ras was causally related to manumycin-induced apoptosis and showed that the response to manumycin was found to be independent of K-Ras function because membrane association and activation of endogenous K-Ras proteins in terms of GTP loading and ERK activation were unabated following treatment with manumycin. Moreover, blocking p21Ras/Raf-1/MEK/ERK cascade by the expression of a transdominant inhibitory mSOS1 mutant in CHO-IR cells kept cells sensitive to the antiapoptotic action of insulin. Insulin-dependent activation of Akt was blocked by 4 h treatment with manumycin (P < 0.01), a kinetic too rapid to be explained by Ras inhibition. This study suggests that the depletion of short-lived farnesylated proteins by manumycin suppresses the antiapoptotic action of insulin at least in part by disrupting Akt activation but not that of the K-Ras/Raf-1/ERK-dependent cascade.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Park D,Pandey SK,Maksimova E,Kole S,Bernier Mdoi
10.1021/bi000995ysubject
Has Abstractpub_date
2000-10-17 00:00:00pages
12513-21issue
41eissn
0006-2960issn
1520-4995pii
bi000995yjournal_volume
39pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Proton NMR spectroscopy was applied to myoglobin in the ferric, water-liganded form (metMbH2O) and the apo form (apoMb) to probe the structure and stability of the latter. Proteins from sperm whale and horse skeletal muscles were studied to simplify the spectral assignment task. Nuclear Overhauser effects and the resp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00143a018
更新日期：1992-07-21 00:00:00
abstract：:In order to investigate the changes of protonation or environment of carboxylic residues occurring upon photoreduction of the secondary quinone acceptor (QB) in the reaction center (RC) of the photosynthetic bacteria Rhodobacter sphaeroides 2.4.1., we have performed light-induced Fourier transform infrared (FTIR) spec...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00045a013
更新日期：1995-11-14 00:00:00
abstract：:The EcoRV restriction endonuclease cleaves DNA at its recognition sequence more readily with Mg2+ as the cofactor than with Mn2+ but, at noncognate sequences that differ from the EcoRV site by one base pair, Mn2+ gives higher rates than Mg2+. A mutant of EcoRV, in which an isoleucine near the active site was replaced ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9523926
更新日期：1996-02-13 00:00:00
abstract：:The human immunodeficiency virus utilizes its gp41 fusion protein to mediate virus-cell membrane fusion. The conserved disulfide loop region in the gp41 hairpin conformation reverses the protein chain, such that the N-terminal heptad repeat and the C-terminal heptad repeat regions interact to form the six-helix bundle...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300868f
更新日期：2012-09-04 00:00:00
abstract：:Doublet signal and singlet-like signal induced in Ca(2+)-depleted PS II were investigated by pulsed EPR in one-dimensionally oriented photosystem (PS) II membranes. The doublet signal showed marked angular dependent change in its spectrum in term of the applied magnetic field, indicating that the magnetic dipole-dipol...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9929406
更新日期：2000-09-12 00:00:00
abstract：:Ribonucleotide reductase (RDPR) from Escherichia coli is composed of two subunits, R1 and R2, both of which are required to catalyze the conversion of nucleotides to deoxynucleotides. This reduction process is accompanied by oxidation of two cysteines within the active site to a disulfide. One of these putative active...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00155a030
更新日期：1992-10-13 00:00:00
abstract：:Acyclovir triphosphate, ganciclovir triphosphate and penciclovir triphosphate inhibited DNA polymerases alpha, delta, and epsilon. Each triphosphate preferentially inhibited pol delta, although ganciclovir triphosphate was the most impressive of the three; the Ki for inhibition of pol delta was 2 microM (competitive w...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00008a014
更新日期：1995-02-28 00:00:00
abstract：:The pyridoxal 5'-phosphate dependent histidine decarboxylase from Morganella morganii shows a nitrogen isotope effect k14/k15 = 0.9770 +/- 0.0021, a carbon isotope effect k12/k13 = 1.0308 +/- 0.0006, and a carbon isotope effect for L-[alpha-2H]histidine of 1.0333 +/- 0.0001 at pH 6.3, 37 degrees C. These results indic...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00416a016
更新日期：1988-08-09 00:00:00
abstract：:Factor XII was purified approximately 14 000-fold from bovine plasma by ammonium sulfate fractionation followed by heparin-agarose, DEAE-Sephadex, CM-cellulose, arginine-agarose, and benzamidine-agarose column chromatography. By this method, about 15 mg of protein was purified from 15 L of plasma with an overall yield...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00629a036
更新日期：1977-05-17 00:00:00
abstract：:The reduced hydroxylase component (MMOH) of soluble methane monooxygenase (MMO) from Methylosinus trichosporium OB3b reacts with O2 and CH4 to produce CH3OH and H2O in a single-turnover reaction. Transient kinetic analysis of this reaction has revealed at least five and probably six intermediates during the turnover [...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960596w
更新日期：1996-08-06 00:00:00
abstract：:A single histidine residue has been placed at either the N-terminus or the C-terminus of each of the two alpha-helices of barnase. The pKa of that histidine residue in each of the four mutants has been determined by 1H NMR. The pKas of the two residues at the C-terminus are, on average, 0.5 unit higher, and those of t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00123a006
更新日期：1992-03-03 00:00:00
abstract：:SH2 domains are protein modules which interact with specific tyrosine phosphorylated sequences in target proteins. The SH2 domain of the Src kinase binds with high affinity to a tyrosine phosphorylated peptide containing the amino acids Glu, Glu, and Ile (EEI) at the positions +1, +2, and +3 C-terminal to the phosphot...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982974y
更新日期：1999-04-20 00:00:00
abstract：:This work shows that the voltage across membranes in two very different preparations, lipid vesicles in suspension and individual HeLa cells under a microscope, is linearly related to the ratio of fluorescence excited from the two wings of the absorption spectrum of a voltage-sensitive dye. The dye di-4-ANEPPS [1-(3-s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00437a003
更新日期：1989-05-30 00:00:00
abstract：:Extended illumination slows the rebinding of CO to myoglobin after photodissociation at cryogenic temperatures. Two types of models have been put forward to explain the effect: motions of the CO within the heme pocket or conformational transitions of the protein. To resolve this ambiguity, we have studied the effect o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00249a030
更新日期：1994-11-15 00:00:00
abstract：:We have performed a kinetic and thermodynamic analysis of 3D(pol) derivatives containing substitutions in the ribose-binding pocket with ATP analogues containing correct and incorrect sugar configurations. We find that Asp-238, a residue in structural motif A that is conserved in all RNA-dependent RNA polymerases, is ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035429s
更新日期：2004-05-11 00:00:00
abstract：:The receptor for atrial natriuretic peptide (ANP) is a type-I transmembrane protein containing an extracellular ligand-binding domain, a single transmembrane sequence, an intracellular kinase-homologous domain, and a guanylate cyclase (GCase) domain. Binding of ANP to the extracellular domain causes activation of the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982127v
更新日期：1999-01-12 00:00:00
abstract：:Recent studies of proteins with reversed charged residues have demonstrated that electrostatic interactions on the surface can contribute significantly to protein stability. We have used the approach of reversing negatively charged residues using Arg to evaluate the effect of the electrostatics context on the transiti...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0354684
更新日期：2004-04-06 00:00:00
abstract：:The formation of a complex between p21(ras) and GAP accelerates the GTPase reaction of p21(ras) and terminates the signal for cell proliferation. The understanding of this rate acceleration is important for the elucidation of the role of Ras mutants in tumor formation. In principle there are two main options for the o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000640e
更新日期：2000-08-15 00:00:00
abstract：:A role for Tyr254 in L-lactate dehydrogenation catalyzed by flavocytochrome b2 has recently been proposed on the basis of the known active-site structure and of studies that had suggested a mechanism involving the initial formation of a lactate carbanion [Lederer, F., & Mathews, F.S. (1987) in Flavins and Flavoprotein...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00479a008
更新日期：1990-07-10 00:00:00
abstract：:The ferritin IRE, a highly conserved (96-99% in vertebrates) mRNA translation regulatory element in animal mRNA, was studied by molecular modeling (using MC-SYM and DOCKING) and by NMR spectroscopy. Cobalt(III) hexammine was used to model hydrated Mg2+. IRE isoforms in other mRNAs regulate mRNA translation or stabilit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9719814
更新日期：1998-02-10 00:00:00
abstract：:Type VI collagen, prepared from guanidine extracts of human amnion, contains very little monomeric material, the major forms being dimers and tetramers. In order to study the orientation of the molecules in these aggregates, they were digested with pepsin followed by bacterial collagenase. Two fragments were isolated,...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00435a020
更新日期：1989-05-02 00:00:00
abstract：:Class A beta-lactamases hydrolyze penicillins and other beta-lactams via an acyl-enzyme catalytic mechanism. Ser70 is the active site nucleophile. By constructing the S70A mutant, which is unable to form the acyl-enzyme intermediate, it was possible to make stable ES complexes with various substrates. The stability of...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9928041
更新日期：2000-05-30 00:00:00
abstract：:The observed regioselectivity of the homolytic cleavage of S-adenosylmethionine (SAM) by the radical SAM enzymes is modeled by free radical displacement reactions at sulfoxide centers. These displacements are also regioselective, in direct consequence of the reaction mechanism. The selectivity in the radical SAM react...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101509u
更新日期：2010-12-28 00:00:00
abstract：:The relationship between cholesterol and ubiquinone synthesis in rat intestinal epithelial cell cultures was examined by using 3 beta-[2-(diethylamino)ethoxy]androst-5-en-17-one hydrochloride (U18666A). Addition of U18666A to cells caused a greater than 90% inhibition of incorporation of [3H]acetate into cholesterol a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00294a001
更新日期：1983-12-06 00:00:00
abstract：:Enzyme IIA(Glc) (EIIA(Glc)) of the phosphoenolpyruvate phosphotransferase system for the uptake of glucose in Escherichia coli and Salmonella inhibits the maltose ATP-binding cassette transporter (MalE-FGK2) by interaction with the nucleotide-binding and -hydrolyzing subunit MalK, a process termed inducer exclusion. W...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00721
更新日期：2016-09-27 00:00:00
abstract：:To probe attitudinal features of the Ca(2+)-deficient site (site I) in the Ca2+ switch of cardiac troponin C (cTnC), we have examined steady-state fluorescence emission and polarization of a Trp26 inserted in a recombinant cardiac TnC (cTnC3.W) and compared these with the properties of the Ca(2+)-competent site I in s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00002a022
更新日期：1995-01-17 00:00:00
abstract：:The solution conformation of the DNA duplex d(C1G2C3A4C5L6C7A8C9G10C11).d(G12C13G14T15G16T17G18T19G20C21G22 ) containing the 2'-deoxyribonolactone lesion (L6) in the middle of the sequence has been investigated by NMR spectroscopy and restrained molecular dynamics calculations. Interproton distances have been obtained...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982743r
更新日期：1999-03-30 00:00:00
abstract：:A procedure has been developed to purify foot and mouth disease virus (FMDV) VP1 surface antigens from recombinant Escherichia coli. The VP1 antigens are expressed as fusion proteins derived from the E. coli Trp operon and VP1 surface protein of FMDV. The procedure is capable of recovering greater than 96% of the desi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00321a031
更新日期：1984-12-18 00:00:00
abstract：:We developed a general strategy for labeling expressed membrane proteins with a peptide epitope tag and detecting the tagged proteins in native cellular membranes. First, we genetically encoded the unnatural amino acid p-azido-L-phenylalanine (azF) at various specific sites in a G protein-coupled receptor (GPCR), C-C ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi301292h
更新日期：2013-02-12 00:00:00
abstract：:Eta-crystallin is a retinal dehydrogenase that has acquired a role as a structural protein in the eye lens of elephant shrews, members of an ancient order of mammals. While it retains some activity toward retinal, which is oxidized to retinoic acid, the protein has acquired a number of specific sequence changes that h...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi027367w
更新日期：2003-04-22 00:00:00
