Purification and immunogenicity of fusion VP1 protein of foot and mouth disease virus.

abstract：:Decarboxylated S-adenosylmethionine was found to be a substrate for the nuclear acetyltransferases that act on polyamines and on histones. The rate of acetylation of decarboxylated S-adenosylmethionine was more than twice that of spermidine at saturating substrate concentrations, and decarboxylated S-adenosylmethionin...

journal_title：Biochemistry

authors： Pegg AE,Wechter RS,Clark RS,Wiest L,Erwin BG

更新日期：1986-01-28 00:00:00

abstract：:Ppr from the purple phototrophic bacterium, Rhodospirillum centenum (also known as Rhodocista centenaria), is a hybrid of photoactive yellow protein (PYP), bacteriophytochrome (Bph), and histidine kinase (HK) domains. The holo-Ppr (containing both chromophores) exhibits characteristic absorption maxima at 435 nm due t...

journal_title：Biochemistry

authors： Kyndt JA,Fitch JC,Meyer TE,Cusanovich MA

更新日期：2007-07-17 00:00:00

abstract：:Sulfation is a Golgi-specific modification of secretory proteins. We have characterized the proteins that are labeled with 35SO4 in cultures of rat hepatocytes and studied their transport to the medium. Analysis by polyacrylamide gel electrophoresis showed that of the five most heavily labeled proteins, four had well-...

journal_title：Biochemistry

authors： von Würtemberg MM,Fries E

更新日期：1989-05-02 00:00:00

abstract：:Typical of many transcriptional regulatory proteins, the lambdoid bacteriophage repressors bind cooperatively to multiple sites on DNA. This cooperative binding is essential for establishment and maintenance of phage lysogeny. In the phage, two repressor homodimers, one bound at each of the adjacent operator sites, in...

journal_title：Biochemistry

authors： Ciubotaru M,Koudelka GB

更新日期：2003-04-15 00:00:00

abstract：:The duplex formed by annealing the formacetal backbone modified dodecamer d-(CGCGTTOCH2OTTGCGC) to its complementary strand, d(GCGCAAAACGCG) (duplex I), has been studied by NMR techniques and analyzed with reference to its unmodified counterpart (duplex II). Comparison of parameters such as 2D cross-peak intensities, ...

journal_title：Biochemistry

authors： Gao X,Brown FK,Jeffs P,Bischofberger N,Lin KY,Pipe AJ,Noble SA

更新日期：1992-07-14 00:00:00

abstract：:Microtubules, ordinarily cold-labile structures, are made entirely resistant to cold temperature by the presence of substoichiometric amounts of STOP (stable tubule only polypeptide), a microtubule-associated protein. We have produced a monoclonal antibody which specifically recognizes a 145-kDa protein previously imp...

journal_title：Biochemistry

authors： Pirollet F,Rauch CT,Job D,Margolis RL

更新日期：1989-01-24 00:00:00

abstract：:Six structural repeat motifs of 58 amino acids are found in the sequence of both mouse and human properdins. Twelve more examples of the motif are available from the sequences of thrombospondin, the terminal complement components, and the thrombospondin-related anonymous protein. The averaged Robson and Chou-Fasman se...

journal_title：Biochemistry

authors： Perkins SJ,Nealis AS,Haris PI,Chapman D,Goundis D,Reid KB

更新日期：1989-09-05 00:00:00

abstract：:The kinetics of human polymerase beta (pol beta) binding to the single-stranded DNA, in the (pol beta)(16) and (pol beta)(5) binding modes, that differ in the number of occluded nucleotide residues in the protein-DNA complexes, have been examined, using the fluorescence stopped-flow technique. This is the first determ...

journal_title：Biochemistry

authors： Rajendran S,Jezewska MJ,Bujalowski W

更新日期：2001-10-02 00:00:00

abstract：:Manganese peroxidase (MnP) from Phanerochaete chrysosporium undergoes a pH-dependent conformational change evidenced by changes in the electronic absorption spectrum. This high- to low-spin alkaline transition occurs at approximately 2 pH units lower in an F190I mutant MnP when compared to the wild-type enzyme. Herein...

journal_title：Biochemistry

authors： Youngs HL,Moënne-Loccoz P,Loehr TM,Gold MH

更新日期：2000-08-15 00:00:00

abstract：:We have studied the photoreaction of 8-methoxypsoralen (8-MOP), 4,5',8-trimethylpsoralen (TMP), and 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen (HMT) with a pair of 18-base-long oligonucleotides in which a 14-base region is complementary. Only one 5'TpA site, favored for both monoadduct and cross-link formation with p...

journal_title：Biochemistry

authors： Yeung AT,Jones BK,Chu CT

更新日期：1988-05-03 00:00:00

abstract：:A protocol for complete isotopic labeling of iso-1-cytochrome c from the eukaryote Saccharomyces cerevisiae is reported. Assignments are reported for the vast majority of the 15N amide resonances in both oxidized and reduced states. 15N heteronuclear relaxation experiments were collected to study the picosecond-nanose...

journal_title：Biochemistry

authors： Fetrow JS,Baxter SM

更新日期：1999-04-06 00:00:00

abstract：:The three-dimensional chalice-like crystal structure of initiation factor 2 IF2/eIF5B from Methanobacterium thermoautotrophicum represents a novel fold and domain architecture in which the N-terminal G domain and the C-terminal C domain are separated by an approximately 40 A alpha-helix. Homologous Thermus thermophilu...

journal_title：Biochemistry

authors： Zoldák G,Sedlák E,Wolfrum A,Musatov A,Fedunová D,Szkaradkiewicz K,Sprinzl M

更新日期：2008-04-29 00:00:00

abstract：:Complex I of respiratory chains transfers electrons from NADH to ubiquinone, coupled to the translocation of protons across the membrane. Two alternative coupling mechanisms are being discussed, redox-driven or conformation-driven. Using "zero-length" cross-linking reagent and isolated hydrophilic domains of complex I...

journal_title：Biochemistry

authors： Berrisford JM,Thompson CJ,Sazanov LA

更新日期：2008-09-30 00:00:00

abstract：:In a previously determined minimal kinetic scheme for DNA polymerization catalyzed by the Klenow fragment (KF) of Escherichia coli DNA polymerase I, a nonchemical step that interconverted the KF'.DNAn+1.PPi and KF.DNAn+1PPi complexes was not observed in correct incorporation [Kuchta, R. D., Mizrahi, V., Benkovic, P.A....

journal_title：Biochemistry

authors： Dahlberg ME,Benkovic SJ

更新日期：1991-05-21 00:00:00

abstract：:Bacillus subtilis CsoR (Bsu CsoR) is a copper-sensing transcriptional repressor that regulates the expression of the copZA operon encoding a copper chaperone and a Cu efflux P-type ATPase, respectively. Bsu CsoR is a homologue of Mycobacterium tuberculosis CsoR (Mtb CsoR), representative of a large Cu(I)-sensing regul...

journal_title：Biochemistry

authors： Ma Z,Cowart DM,Scott RA,Giedroc DP

更新日期：2009-04-21 00:00:00

abstract：:Calmodulin (CaM) is an essential, eukaryotic protein comprised of two highly homologous domains (N and C). CaM binds four calcium ions cooperatively, regulating a wide array of target proteins. A genetic screen of Paramecia by Kung [Kung, C. et al. (1992) Cell Calcium 13, 413-425] demonstrated that the domains of CaM ...

journal_title：Biochemistry

authors： VanScyoc WS,Newman RA,Sorensen BR,Shea MA

更新日期：2006-12-05 00:00:00

abstract：:We have investigated the topology of the band 3 protein of the human erythrocyte membrane by a combination of chemical labeling and proteolytic cleavage. The N-terminal third of the membrane-bound domain of band 3 is a 17 000-dalton chymotryptic fragment that is known to traverse the membrane an odd number of times. A...

journal_title：Biochemistry

authors： Jennings ML,Nicknish JS

更新日期：1984-12-18 00:00:00

abstract：:This paper characterizes the low-affinity ligand binding interactions of a fluorinated volatile anesthetic, isoflurane (CHF2OCHClCF3), with bovine serum albumin (BSA) using 19F-NMR transverse relaxation (T2). 19F-NMR spectra of isoflurane in aqueous BSA reveal a single isoflurane trifluoromethyl resonance, indicative ...

journal_title：Biochemistry

authors： Dubois BW,Evers AS

更新日期：1992-08-11 00:00:00

abstract：:The goal of this study was to establish conditions for solubilization and characterization of CPTo, the malonyl-CoA sensitive form of mitochondrial carnitine palmitoyltransferase. CPTo of heart mitochondria is soluble in 1% octyl glucoside with retention of malonyl-CoA sensitivity. The degree of malonyl-CoA sensitivit...

journal_title：Biochemistry

authors： Kerner J,Bieber L

更新日期：1990-05-08 00:00:00

abstract：:The bovine adrenodoxin gene gives rise to two species of mRNA differing only at their 5'-ends. The synthesis of these two types of mRNA in bovine adrenal cortical cells is regulated transcriptionally in part by ACTH via the action of cAMP. Examination of the 5'-end of the adrenodoxin gene revealed that each mRNA conta...

journal_title：Biochemistry

authors： Chen JY,Waterman MR

更新日期：1992-03-03 00:00:00

abstract：:The Escherichia coli siderophore enterobactin is assembled from 2,3-dihydroxybenzoate (2,3-DHB) and l-serine by the nonribosomal peptide synthetases EntB and EntF. The processive thiol-template strategy used can be sabotaged by EntB misacylation. Through in vitro kinetic analysis, we demonstrate two potential routes t...

journal_title：Biochemistry

authors： Chen D,Wu R,Bryan TL,Dunaway-Mariano D

更新日期：2009-01-27 00:00:00

abstract：:The spin label Tempo-maleimide, when "immobilized" in hemoglobin, is shown to exhibit motional fluctuation whose amplitude and/or frequency depend on temperature and solution conditions. These motional fluctuations are observable by several electron spin resonance techniques. For desalted hemoglobin the fluctuations a...

journal_title：Biochemistry

authors： Johnson ME

更新日期：1978-04-04 00:00:00

abstract：:Glycosylation is a common modification that can endow proteins with altered physical and biological properties. Ribonuclease 1 (RNase 1), which is the human homologue of the archetypal enzyme RNase A, undergoes N-linked glycosylation at asparagine residues 34, 76, and 88. We have produced the three individual glycofor...

journal_title：Biochemistry

authors： Kilgore HR,Latham AP,Ressler VT,Zhang B,Raines RT

更新日期：2020-09-01 00:00:00

abstract：:Mutations in the gene encoding human ATP:cobalamin adenosyltransferase (hATR) can result in the metabolic disorder known as methylmalonic aciduria (MMA). This enzyme catalyzes the final step in the conversion of cyanocobalamin (vitamin B12) to the essential human cofactor adenosylcobalamin. Here we present the 2.5 A c...

journal_title：Biochemistry

authors： Schubert HL,Hill CP

更新日期：2006-12-26 00:00:00

abstract：:A general protocol for preparation of oligonucleotides containing intrastrand cross-links between the exocyclic amino groups of adjacent deoxyguanosines has been developed. A series of 2, 3, and 4 methylene cross-links was incorporated site-specifically into an 11-mer (5'-GGCAGGTGGTG-3', cross-linked positions are und...

journal_title：Biochemistry

authors： Kowalczyk A,Carmical JR,Zou Y,Van Houten B,Lloyd RS,Harris CM,Harris TM

更新日期：2002-03-05 00:00:00

abstract：:The Rhodnius prolixus aggregation inhibitor 1 (RPAI-1) is a novel blood-sucking salivary molecule that binds to ADP and attenuates platelet aggregation. In this report, we determine the binding constants and specificity of RPAI-1 for adenine nucleotides and its functional significance. By the Hummel-Dreyer method of e...

journal_title：Biochemistry

authors： Francischetti IM,Andersen JF,Ribeiro JM

更新日期：2002-03-19 00:00:00

abstract：:The recently isolated Escherichia coli murB gene (Pucci et al., 1992) has been cloned into an expression vector and the encoded UDP-N-acetylenolpyruvylglucosamine reductase (EC 1.1.1.158) was overproduced to about 10% of soluble cell protein. The encoded 38-kDa protein has been purified to near homogeneity. It was fou...

journal_title：Biochemistry

authors： Benson TE,Marquardt JL,Marquardt AC,Etzkorn FA,Walsh CT

更新日期：1993-03-02 00:00:00

abstract：:The navel orangeworm, Amyelois transitella (Walker), is an agricultural insect pest that can be controlled by disrupting male-female communication with sex pheromones, a technique known as mating disruption. Insect pheromone-binding proteins (PBPs) provide fast transport of hydrophobic pheromones through the aqueous s...

journal_title：Biochemistry

authors： Xu X,Xu W,Rayo J,Ishida Y,Leal WS,Ames JB

更新日期：2010-02-23 00:00:00

abstract：:The Staphylococcus aureus sortase transpeptidase SrtA isoform is responsible for the covalent attachment of virulence and colonization-associated proteins to the bacterial peptidoglycan. SrtA utilizes two substrates, undecaprenol-pyrophosphoryl-MurNAc(GlcNAc)-Ala-D-isoGlu-Lys(epsilon-Gly(5))-D-Ala-D-Ala (branched Lipi...

journal_title：Biochemistry

authors： Kruger RG,Otvos B,Frankel BA,Bentley M,Dostal P,McCafferty DG

更新日期：2004-02-17 00:00:00

abstract：:Exoenzyme S (ExoS) is a mono-ADP-ribosyltransferase secreted by the opportunistic pathogen Pseudomonas aeruginosa. ExoS requires a eukaryotic factor, the 14-3-3 protein, for enzymatic activity. Here, two aspects of the activation of the ADP-ribosyltransferase activity of ExoS by 14-3-3 proteins are examined. Initial s...

journal_title：Biochemistry

authors： Zhang L,Wang H,Masters SC,Wang B,Barbieri JT,Fu H

更新日期：1999-09-14 00:00:00