Hypochlorous acid reacts with the N-terminal methionines of proteins to give dehydromethionine, a potential biomarker for neutrophil-induced oxidative stress.

abstract：:(S)-Mandelate dehydrogenase from Pseudomonas putida belongs to a FMN-dependent enzyme family that oxidizes (S)-alpha-hydroxyacids. Despite a high degree of sequence and structural similarity, this family can be divided into three subgroups based on the different oxidants utilized in the second oxidative half-reaction....

journal_title：Biochemistry

authors： Dewanti AR,Xu Y,Mitra B

更新日期：2004-08-24 00:00:00

abstract：:The substrate-bound form of the enzyme heme oxygenase (HO), which catalyzed the stereospecific alpha-meso bridge cleavage of hemin to yield biliverdin IX alpha, has been investigated by 1H NMR in both its primarily high-spin and its cyanide-inhibited low-spin forms. Both derivatives yield 1H NMR spectra indicative of ...

journal_title：Biochemistry

authors： Hernández G,Wilks A,Paolesse R,Smith KM,Ortiz de Montellano PR,La Mar GN

更新日期：1994-05-31 00:00:00

abstract：:We describe the synthesis of bordifluoropyrromethene (BODIPY), fluorescein, and related fluorescent derivatives of the beta-adrenergic ligand CGP 12177. With these probes we screened insect (Sf9) cells stably transformed with the human beta 2-adrenoceptor gene and expressing (2-3.5) x 10(5) human beta 2-adrenoceptors ...

journal_title：Biochemistry

authors： Heithier H,Hallmann D,Boege F,Reiländer H,Dees C,Jaeggi KA,Arndt-Jovin D,Jovin TM,Helmreich EJ

更新日期：1994-08-09 00:00:00

abstract：:Histone deacetylases have proven to be attractive novel targets for the treatment of cancer. The first inhibitor of histone deacetylases was approved for the treatment of cutaneous T-cell lymphoma in 2006. The identification of new lead structures with improved effectiveness and fewer side effects is necessary. This r...

journal_title：Biochemistry

authors： Sykora J,Meyer-Almes FJ

更新日期：2010-02-23 00:00:00

abstract：:The value of reducing the activity of ornithine decarboxylase (ODC), a key enzyme in the biosynthesis of polyamines, is well-appreciated. Polyamines are necessary components for cell growth, and manipulation of polyamine homeostasis may be an effective strategy for the treatment of a number of disorders, including neo...

journal_title：Biochemistry

authors： Ackermann JM,Kanugula S,Pegg AE

更新日期：2005-02-15 00:00:00

abstract：:In all structurally characterized bacterial multicomponent monooxygenase (BMM) hydroxylase proteins, a series of hydrophobic cavities in the α-subunit trace a conserved path from the protein exterior to the carboxylate-bridged diiron active site. This study examines these cavities as a potential route for transport of...

journal_title：Biochemistry

authors： McCormick MS,Lippard SJ

更新日期：2011-12-27 00:00:00

abstract：:Aminoacyl-tRNA synthetases ensure the fidelity of protein synthesis by accurately selecting and activating cognate amino acids for aminoacylation of the correct tRNA. Some tRNA synthetases have evolved an editing active site that is separate from the amino acid activation site providing two steps or "sieves" for amino...

journal_title：Biochemistry

authors： Mursinna RS,Lincecum TL Jr,Martinis SA

更新日期：2001-05-08 00:00:00

abstract：:We have determined the nucleotide sequence and the derived amino acid sequence of the phosphoprotein-encoding region of the chicken vitellogenin III gene. The sequence of this minor vitellogenin could be aligned with exon 22 up to exon 27 of the previously sequenced major vitellogenin II gene (van het Schip et al., 19...

journal_title：Biochemistry

authors： Byrne BM,de Jong H,Fouchier RA,Williams DL,Gruber M,Ab G

更新日期：1989-03-21 00:00:00

abstract：:The carbon-13 nuclear magnetic resonance (13C NMR) spectra of luteinizing hormone-releasing hormone (LH-RH) and lower homologous peptides have been assigned in aqueous solutions at various pH values. 13C spin-lattice relaxation times (T1) have been measured for all proton-bearing carbons at 25.2 and 67.9 MHz. From the...

journal_title：Biochemistry

authors： Deslauriers R,Levy GC,McGregor WH,Sarantakis K,Smith IC

更新日期：1975-09-23 00:00:00

abstract：:Transferrin exhibits heterogeneity in glycosylation characteristic of pathological changes in alcohol abuse and congenital disorders in glycosylation. This study investigated an alternative approach in the detection of carbohydrate-deficient transferrin based on the premise that glycosylation may afford some degree of...

journal_title：Biochemistry

authors： Valmu L,Kalkkinen N,Husa A,Rye PD

更新日期：2005-12-13 00:00:00

abstract：:Base excision repair is a major pathway for the removal of simple lesions in DNA including base damage and base loss (abasic site). Base excision repair requires the coordinated action of several repair and ancillary proteins, the impairment of which can lead to genetic instability. Using a protein-DNA cross-linking a...

journal_title：Biochemistry

authors： Parsons JL,Dianova II,Allinson SL,Dianov GL

更新日期：2005-08-09 00:00:00

abstract：:Whereas the salt-dependent conformational transition of acid-denatured horse ferricytochrome c at pH 2 is approximated by a two-state mechanism from the acid-unfolded state to the molten globule state [Kataoka, M., Hagihara, Y., Mihara, K., & Goto, Y. (1993) J. Mol. Biol. 229, 591-596], the corresponding transition in...

journal_title：Biochemistry

authors： Goto Y,Hagihara Y,Hamada D,Hoshino M,Nishii I

更新日期：1993-11-09 00:00:00

abstract：:Ethylene and its analogues acetylene, carbon monoxide, and propylene inhibited the rate of oxidation of indole-3-acetic acid by peroxidase. Annulment of this effect by addition of superoxide dismutase showed that inhibition occurred only in the presence of the superoxide anion radical (O2-.). Kinetic and spectral data...

journal_title：Biochemistry

authors： Smith AM,Morrison WL,Milham PJ

更新日期：1983-03-29 00:00:00

abstract：:We have studied the Cu(II), Co(II), and Fe(III) complexes of the antineoplastic drug bleomycin by using electron spin--echo envelope spectroscopy. For all three complexes, nitrogen coordination of the metal ions is demonstrated. For the Cu(II)-- and Co(II)--drug complexes, we have been able to identify imidazole as a ...

journal_title：Biochemistry

authors： Burger RM,Adler AD,Horwitz SB,Mims WB,Peisach J

更新日期：1981-03-17 00:00:00

abstract：:We have studied the physical and functional linkages of heterologously expressed human 5-HT1A receptors to G protein alpha-subunits in HeLa and CHO-K1 cells. HeLa cells expressed immunoreactivity to G(i) proteins with an apparent rank order of G(i) alpha 3 (approximately 1 pmol/mg of protein) > G(i) alpha 1 (approxima...

journal_title：Biochemistry

authors： Raymond JR,Olsen CL,Gettys TW

更新日期：1993-10-19 00:00:00

abstract：:Aflatoxin B1 (AFB1) exo-8,9-epoxide, the reactive product of the hepatocarcinogen AFB1, is stable in aprotic solvents but hydrolyzes rapidly in H2O at 25 degrees C and pH 7 (t1/2 = 1 s). However, it is also known that some glutathione (GSH) transferases can conjugate the epoxide with GSH to give the adduct in high yie...

journal_title：Biochemistry

authors： Johnson WW,Ueng YF,Widersten M,Mannervik B,Hayes JD,Sherratt PJ,Ketterer B,Guengerich FP

更新日期：1997-03-18 00:00:00

abstract：:Spectroscopic methods are used to probe the interactions of several anilinoacridine analogues with calf thymus DNA over a wide range of temperatures and sodium chloride concentrations. The structurally similar compounds m-AMSA, AMSA (both active as antitumor agents), and o-AMSA (inactive as an antitumor agent) have be...

journal_title：Biochemistry

authors： Wadkins RM,Graves DE

更新日期：1991-04-30 00:00:00

abstract：:Murine cDNA that encodes neuromodulin, a neurospecific calmodulin binding protein, was inserted into the plasmid pKK223-3 for expression in Escherichia coli. After being transformed into E. coli strain SG20252 (lon-), the expression vector directed the synthesis of a protein that was recognized by polyclonal antibodie...

journal_title：Biochemistry

authors： Au DC,Apel ED,Chapman ER,Estep RP,Nicolson TA,Storm DR

更新日期：1989-10-03 00:00:00

abstract：:Cystathionine gamma-synthase (CGS) is a pyridoxal phosphate-dependent enzyme that catalyzes a gamma-replacement reaction, in which the succinyl group of an O-succinyl-L-homoserine (L-OSHS) is displaced by the thiol of L-cysteine to form L-cystathionine, in the first step of the bacterial transsulfuration pathway. The ...

journal_title：Biochemistry

authors： Aitken SM,Kim DH,Kirsch JF

更新日期：2003-09-30 00:00:00

abstract：:Multivalent binding is an efficient means to enhance the affinity and specificity of chemical probes targeting multidomain proteins in order to study their function and role in disease. While the theory of multivalent binding is straightforward, physical and structural characterization of bivalent binding encounters m...

journal_title：Biochemistry

authors： Suh JL,Watts B,Stuckey JI,Norris-Drouin JL,Cholensky SH,Dickson BM,An Y,Mathea S,Salah E,Knapp S,Khan A,Adams AT,Strahl BD,Sagum CA,Bedford MT,James LI,Kireev DB,Frye SV

更新日期：2018-04-10 00:00:00

abstract：:Structurally diverse small molecules, including 5-(2-benzofuryl)-4-phenyl-1,2,4-triazole-3-thiol (BETT), have been identified via high-throughput screening as activators of caspase-3 in HeLa cell extracts. However, little is known about their mechanism of action. In this study, we investigate how BETT regulates prothy...

journal_title：Biochemistry

authors： Qi X,Wang L,Du F

更新日期：2010-03-09 00:00:00

abstract：:Ppr from the purple phototrophic bacterium, Rhodospirillum centenum (also known as Rhodocista centenaria), is a hybrid of photoactive yellow protein (PYP), bacteriophytochrome (Bph), and histidine kinase (HK) domains. The holo-Ppr (containing both chromophores) exhibits characteristic absorption maxima at 435 nm due t...

journal_title：Biochemistry

authors： Kyndt JA,Fitch JC,Meyer TE,Cusanovich MA

更新日期：2007-07-17 00:00:00

abstract：:We previously isolated a unique anticoagulant protein named IX/X-bp (factor IX/factor X-binding protein) from the venom of the habu snake Trimeresurus flavoviridis. We recently determined its primary structure and found that this protein had a structure homologous to the carbohydrate-recognition domains of C-type lect...

journal_title：Biochemistry

authors： Sekiya F,Atoda H,Morita T

更新日期：1993-07-13 00:00:00

abstract：:We have studied the properties and structures of a series of Escherichia coli RNA polymerase ternary complexes formed during the initial steps of RNA chain initiation and elongation. Five different templates were used that contained the bacteriophage T7 A1 promoter or the E. coli Tac or the lac UV5 promoter, as well a...

journal_title：Biochemistry

authors： Krummel B,Chamberlin MJ

更新日期：1989-09-19 00:00:00

abstract：:Polyalanine (polyA) is the third-most prevalent homopeptide repeat in eukaryotes, behind polyglutamine and polyasparagine. Abnormal expansion of the polyA repeat is linked to at least nine human diseases, and the disease mechanism likely involves enhanced length-dependent aggregation. Because of the simplicity of its ...

journal_title：Biochemistry

authors： Bernacki JP,Murphy RM

更新日期：2011-11-01 00:00:00

abstract：:The unfolding and refolding properties of human lysozyme and two amyloidogenic variants (Ile56Thr and Asp67His) have been studied by stopped-flow fluorescence and hydrogen exchange pulse labeling coupled with mass spectrometry. The unfolding of each protein in 5.4 M guanidine hydrochloride (GuHCl) is well described as...

journal_title：Biochemistry

authors： Canet D,Sunde M,Last AM,Miranker A,Spencer A,Robinson CV,Dobson CM

更新日期：1999-05-18 00:00:00

abstract：:Purine C-8 tritium-labeling rates have been measured at specific sites in Escherichia coli tRNAIle and tRNA2Tyr. The results are compared with those obtained for yeast tRNAPhe (preceding paper(Gamble et al., 1976)). The tRNAIle and tRNAPhe fall into the same general class of tRNA structures, while tRNA2Tyr is in a dif...

journal_title：Biochemistry

authors： Schoemaker HJ,Gamble RC

更新日期：1976-06-29 00:00:00

abstract：:The globular domain of chicken histone H1 (GH1) has been studied by 1H homonuclear and 1H-15N heteronuclear 2D NMR spectroscopy. After the full assignment of the proton and 15N resonances, the tertiary structure of GH1 was determined by an iterative procedure using distance geometry and restrained simulated annealing....

journal_title：Biochemistry

authors： Cerf C,Lippens G,Ramakrishnan V,Muyldermans S,Segers A,Wyns L,Wodak SJ,Hallenga K

更新日期：1994-09-20 00:00:00

abstract：:We have labeled the primer binding domain of murine leukemia virus reverse transcriptase (MuLV RT) by covalently cross-linking 5' end labeled d(T)8 to MuLV RT, using ultraviolet light energy. The specificity and the functional significance of the primer cross-linking reaction were demonstrated by the fact that (i) oth...

journal_title：Biochemistry

authors： Tirumalai RS,Modak MJ

更新日期：1991-07-02 00:00:00

abstract：:The peptide cyclo(hex-Amb(1)-Cys(2))-Thr(3)-Val(4)-Thr(5)-Nph(6)-NH2 was previously shown to be a slow, tight-binding inhibitor (Ki = 37 nM) of the yeast oligosaccharyl transferase (OT) [Hendrickson et al. (1996) J. Am. Chem. Soc. 118, 7636-7637]. This enzyme catalyzes the transfer of a carbohydrate moiety to an aspar...

journal_title：Biochemistry

authors： Kellenberger C,Hendrickson TL,Imperiali B

更新日期：1997-10-14 00:00:00