Conformational flexibility of luteinizing hormone-releasing hormone in aqueous solution. A carbon-13 spin-lattice relaxation time study.

abstract：:The pre-steady-state kinetics of the microtubule-kinesin ATPase were investigated by chemical-quench flow methods using the Drosophila kinesin motor domain (K401) expressed in Escherichia coli [Gilbert, S. P., & Johnson, K. A. (1993) Biochemistry 32, 4677-4684]. The results define a minimal mechanism: M.K + ATP in equ...

journal_title：Biochemistry

authors： Gilbert SP,Johnson KA

更新日期：1994-02-22 00:00:00

abstract：:Bean-deleterious Pseudomonas A214 produced the extracellular yellow-green, fluorescent siderophore [microbial iron(III) transport agent] pseudobactin A214 under iron-limiting conditions. Pseudobactin A214 has a molecular formula of C46H64N13O22 and a molecular mass of 1151 g/mol. Pseudobactin A214 contained an N-block...

journal_title：Biochemistry

authors： Buyer JS,Wright JM,Leong J

更新日期：1986-09-23 00:00:00

abstract：:The function of the GM2 activator protein is to act as a substrate-specific cofactor in the hydrolysis of GM2 ganglioside by beta-hexosaminidase A. Mutations in the gene encoding it result in the AB variant form of GM2 gangliosidosis. One such mutation, Cys138 Arg, results in the mutant protein being retained and degr...

journal_title：Biochemistry

authors： Xie B,Rigat B,Smiljanic-Georgijev N,Deng H,Mahuran D

更新日期：1998-01-20 00:00:00

abstract：:We propose a rapid chemical strategy for identifying base pairs in structured nucleic acid systems. The approach goes beyond traditional chemical mapping approaches by monitoring perturbations of each residue's chemical accessibility in response to systematic mutagenesis of residues that are distant in sequence but ne...

journal_title：Biochemistry

authors： Kladwang W,Das R

更新日期：2010-09-07 00:00:00

abstract：:Gastric parietal cell apical membranes must protect the cell from the extremely low pH and wide variations in osmolality of the gastric juice. To characterize the permeability properties of gastric apical membranes, we have measured passive permeabilities to water, protons, NH3, and small nonelectrolytes of membrane v...

journal_title：Biochemistry

authors： Priver NA,Rabon EC,Zeidel ML

更新日期：1993-03-16 00:00:00

abstract：:Escherichia coli DNA photolyase contains a stable flavin radical that is readily photoreduced in the presence of added electron donors. Picosecond, nanosecond, and conventional flash photolysis technique have been employed to investigate the events leading to photoreduction from 40 ps to tens of milliseconds following...

journal_title：Biochemistry

authors： Heelis PF,Okamura T,Sancar A

更新日期：1990-06-19 00:00:00

abstract：:Molecular cloning revealed the unique serpin endopin 2C that demonstrates selective inhibition of cathepsin L compared to papain or elastase. Endopin 2C, thus, functions as a serpin with the property of cross-class inhibition. Endopin 2C possesses homology in primary sequence to endopin 2A and other isoforms of endopi...

journal_title：Biochemistry

authors： Hwang SR,Stoka V,Turk V,Hook VY

更新日期：2005-05-31 00:00:00

abstract：:Although the importance of interactions involving both the cytosolic and transmembrane regions of SecY and SecE has been documented, no information has been available for the physical contact sites of these translocase subunits in their cytosolic domains. We now carried out site-specific cross-linking experiments to i...

journal_title：Biochemistry

authors： Satoh Y,Mori H,Ito K

更新日期：2003-06-24 00:00:00

abstract：:In eukaryotes, choline acetyltransferase (ChAT) catalyzes the reversible formation of the neurotransmitter acetylcholine from choline and acetyl-CoA. ChAT belongs to a family of CoA-dependent enzymes that also includes the carnitine acyltransferases CrAT, CrOT, and CPTs. In contrast to CrOT and CPTs that are very acti...

journal_title：Biochemistry

authors： Green KD,Porter VR,Zhang Y,Garneau-Tsodikova S

更新日期：2010-07-27 00:00:00

abstract：:We studied hyperosmolarity-induced changes in cell volume and cytoplasmic pH in PS120 cells expressing Na(+)/H(+) exchanger (NHE) isoforms and their mutants. Change in cell volume was estimated by measuring change in cell height by means of confocal microscopy. Regulatory volume increase (RVI) and cytoplasmic alkalini...

journal_title：Biochemistry

authors： Su X,Pang T,Wakabayashi S,Shigekawa M

更新日期：2003-02-04 00:00:00

abstract：:5'-Nucleotidase has been extracted from rat heart and purified to apparent homogeneity. The enzyme is a glycoprotein. Gel electrophoresis in the presence of sodium dodecyl sulfate indicates that the apparent molecular weight of the subunit is 74 000 at several different gel concentrations. Cross-linking of the native ...

journal_title：Biochemistry

authors： Naito Y,Lowenstein JM

更新日期：1981-09-01 00:00:00

abstract：:An organic solvent soluble polypeptide has been isolated from photoreceptor complexes and chromatophores of Rhodospirillum rubrum. After extraction of the protein from lyophilized samples with 1:1 chloroform-methanol, it was purified by column chromatography. Its isoelectric point determined by isoelectric focusing wa...

journal_title：Biochemistry

authors： Tonn SJ,Gogel GE,Loach PA

更新日期：1977-03-08 00:00:00

abstract：:Using a simple model for a biological membrane we examine cation-induced gel phase formation and the depletion of polypeptide from the gel phase. The model system consists of vesicles of phosphatidic acid and phosphatidylcholine which contain gramicidin A'. By use of electron spin resonance to monitor lipid phase beha...

journal_title：Biochemistry

authors： Feigenson GW

更新日期：1983-06-21 00:00:00

abstract：:The aminoacylase-1/metallopeptidase 20 (Acy1/M20) family features several l-aminoacylases useful in biocatalysis. Mammalian Acy1, in particular, has been applied in racemic resolution and reverse hydrolysis. Despite recent advances in our understanding of the active site architecture and functioning, determinants of A...

journal_title：Biochemistry

authors： Lindner HA,Alary A,Wilke M,Sulea T

更新日期：2008-04-08 00:00:00

abstract：:Translocator protein 18 kDa (TSPO) was previously known as the peripheral benzodiazepine receptor (PBR) in eukaryotes, where it is mainly localized to the mitochondrial outer membrane. Considerable evidence indicates that it plays regulatory roles in steroidogenesis and apoptosis and is involved in various human disea...

journal_title：Biochemistry

authors： Li F,Liu J,Liu N,Kuhn LA,Garavito RM,Ferguson-Miller S

更新日期：2016-05-24 00:00:00

abstract：:The nucleotide binding site of the uncoupling protein (UCP) from brown adipose tissue was mapped by photoaffinity labeling with 2-azidoadenosine 5'-triphosphate (2-azido-ATP) and by affinity labeling with 3'-O-(5-fluoro-2,4-dinitrophenyl)adenosine 5'-triphosphate (FDNP-ATP). Both analogs bind with high affinity and sp...

journal_title：Biochemistry

authors： Mayinger P,Klingenberg M

更新日期：1992-11-03 00:00:00

abstract：:It is now accepted that two or more conformations may exist within the same DNA molecule, thereby generating conformational junctions. The presence of B-Z junctions between right- and left-handed DNA conformations has been detected in plasmids by a number of techniques. Preliminary characterization of the first exampl...

journal_title：Biochemistry

authors： Sheardy RD,Winkle SA

更新日期：1989-01-24 00:00:00

abstract：:The deacetylation of UDP-3-O-[(R)-3-hydroxymyristoyl]-N-acetylglucosamine (UDP-3-O-acyl-GlcNAc) by LpxC is the committed reaction of lipid A biosynthesis. CHIR-090, a novel N-aroyl-l-threonine hydroxamic acid, is a potent, slow, tight-binding inhibitor of the LpxC deacetylase from the hyperthermophile Aquifex aeolicus...

journal_title：Biochemistry

authors： Barb AW,McClerren AL,Snehelatha K,Reynolds CM,Zhou P,Raetz CR

更新日期：2007-03-27 00:00:00

abstract：:Phosphoenolthiopyruvate, the analogue of phosphoenolpyruvate in which the bridging oxygen of the phosphate ester is replaced by sulfur, has been synthesized from methyl acrylate and dimethyl (chlorothio)phosphonate. The compound is a substrate for alkaline phosphatase, pyruvate kinase, enolase, and phosphoenolpyruvate...

journal_title：Biochemistry

authors： Sikkema KD,O'Leary MH

更新日期：1988-02-23 00:00:00

abstract：:Cryospectrokinetic studies of zinc and cobalt carboxypeptidase A disclosed two intermediates in the hydrolysis of both peptides and depsipeptides and furnished all the rate and equilibrium constants for the reaction scheme E + S in equilibrium ES1 in equilibrium ES2---E + P [Auld, D. S., Galdes, A., Geoghegan, K. F., ...

journal_title：Biochemistry

authors： Galdes A,Auld DS,Vallee BL

更新日期：1986-02-11 00:00:00

abstract：:In the cyanobacterium Anabaena, the precursor to tRNA(Leu) has a 249-nucleotide group I intron inserted between the wobble and second bases of the anticodon; the intron self-splices during transcription in vitro [Xu, M. Q., Kathe, S. D., Goodrich-Blair, H., Nierzwicki-Bauer, S. A., & Shub, D. A. (1990) Science 250, 15...

journal_title：Biochemistry

authors： Zaug AJ,McEvoy MM,Cech TR

更新日期：1993-08-10 00:00:00

abstract：:The carbon monoxide binding curve to human hemoglobin A0 has been measured to high precision in experimental conditions of 600 microM heme, 0.1 M N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid, 0.1 M NaCl, 10 mM inositol hexaphosphate, 1 mM disodium ethylenediaminetetraacetic acid, pH 6.94, and 25 degrees C. Co...

journal_title：Biochemistry

authors： Di Cera E,Doyle ML,Connelly PR,Gill SJ

更新日期：1987-10-06 00:00:00

abstract：:The role of the interaction between actin and the secondary actin binding site of myosin (segment 565-579 of rabbit skeletal muscle myosin, referred to as loop 3 in this work) has been studied with proteolytically generated smooth and skeletal muscle myosin subfragment 1 and recombinant Dictyostelium discoideum myosin...

journal_title：Biochemistry

authors： Van Dijk J,Furch M,Lafont C,Manstein DJ,Chaussepied P

更新日期：1999-11-16 00:00:00

abstract：:The X-ray crystal structure of recombinant Desulfovibrio vulgaris rubrerythrin (Rbr) that was subjected to metal constitution first with zinc and then iron, yielding ZnS(4)Rbr, is reported. A [Zn(SCys)(4)] site with no iron and a diiron site with no appreciable zinc in ZnS(4)Rbr were confirmed by analysis of the anoma...

journal_title：Biochemistry

authors： Jin S,Kurtz DM Jr,Liu ZJ,Rose J,Wang BC

更新日期：2004-03-23 00:00:00

abstract：:Exchangeable imidazole NH proton nuclear magnetic resonance (NMR) signals of the proximal histidine F8, which is directly coordinated to the paramagnetic porphyrin cobalt ion, have been measured for cobalt-substituted deoxyhemoglobins (deoxy-CoHb) and iron-cobalt hybrid hemoglobins, alpha (Co)2 beta (Fe)2 and alpha (F...

journal_title：Biochemistry

authors： Inubushi T,Ikeda-Saito M,Yonetani T

更新日期：1983-06-07 00:00:00

abstract：:Betaine-homocysteine S-methyltransferase (BHMT) is a zinc-dependent enzyme that catalyzes the transfer of a methyl group from glycine betaine (Bet) to homocysteine (Hcy) to form dimethylglycine (DMG) and methionine (Met). Previous studies in other laboratories have indicated that catalysis proceeds through the formati...

journal_title：Biochemistry

authors： Castro C,Gratson AA,Evans JC,Jiracek J,Collinsová M,Ludwig ML,Garrow TA

更新日期：2004-05-11 00:00:00

abstract：:The thermodynamics and kinetics of magnesium binding to tRNA(Phe)(yeast) have been studied directly by 25Mg NMR. In 0.17 M Na+(aq), tRNA(Phe) exists in its native conformation and the number of strong binding sites (Ka greater than or equal to 10(4)) was estimated to be 3-4 by titration experiments, in agreement with ...

journal_title：Biochemistry

authors： Reid SS,Cowan JA

更新日期：1990-06-26 00:00:00

abstract：:Peptidylglycine alpha-hydroxylating monooxygenase (PHM) is a copper, ascorbate, and molecular oxygen dependent enzyme that catalyzes the first step leading to the C-terminal amidation of glycine-extended peptides. The catalytic core of PHM (PHMcc), refined to residues 42-356 of the PHM protein, was expressed at high l...

journal_title：Biochemistry

authors： Kolhekar AS,Keutmann HT,Mains RE,Quon AS,Eipper BA

更新日期：1997-09-09 00:00:00

abstract：:Human angiotensin-converting enzyme (ACE) has two homologous domains, the N and C domains, with differing substrate preferences. X-ray crystal structures of the C and N domains complexed with various inhibitors have allowed identification of active site residues that might be important for the molecular basis of this ...

journal_title：Biochemistry

authors： Watermeyer JM,Kröger WL,O'Neill HG,Sewell BT,Sturrock ED

更新日期：2008-06-03 00:00:00

abstract：:Microdomains known as "rafts" have been isolated from many cell types as detergent-resistant membranes (DRMs) and are enriched in sphingolipids and cholesterol. However, there has been considerable controversy over whether such domains are found in native membranes or are artificially generated by the purification pro...

journal_title：Biochemistry

authors： Luria A,Vegelyte-Avery V,Stith B,Tsvetkova NM,Wolkers WF,Crowe JH,Tablin F,Nuccitelli R

更新日期：2002-11-05 00:00:00