Abstract:
:Exchangeable imidazole NH proton nuclear magnetic resonance (NMR) signals of the proximal histidine F8, which is directly coordinated to the paramagnetic porphyrin cobalt ion, have been measured for cobalt-substituted deoxyhemoglobins (deoxy-CoHb) and iron-cobalt hybrid hemoglobins, alpha (Co)2 beta (Fe)2 and alpha (Fe)2 beta (Co)2. Comparison of NMR spectra between these Co-substituted hemoglobins and natural Fe-containing hemoglobin allows the assignment of the NH signals to the specific subunits, namely, a signal at 53.8 ppm to the alpha-subunits and a signal at 58.4 ppm to the beta-subunits for deoxy-CoHb at 23 degrees C, respectively. The coordination of carbon monoxide to the beta-subunits in alpha (Co)2- beta (Fe)2 gave rise to an 8 ppm downfield shift of the proximal histidyl NH signal in the deoxy alpha (Co)2 subunits. In contrast, the coordination of carbon monoxide to the alpha-subunits in alpha (Fe)2 beta (Co)2 showed only a 1 ppm downfield shift for the deoxy beta (Co)2 subunits. These observations suggest that the metal-histidyl coordination bonds in the deoxy cobalt subunits are strengthened by the ligation of carbon monoxide in the counterpart ferrous subunits. However, the change of the coordination was much larger in the alpha (Co)2 subunits than that in the beta (Co)2 subunits. Line-width analysis of the NH signals for CO-bound hybrid hemoglobins gave a mean exchange lifetime of 3.2 X 10(3) S-1 for the T to R quaternary structural transition. The proximal histidyl binding situation at some liganded states is discussed in relation to the quaternary structure of hemoglobin molecules.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Inubushi T,Ikeda-Saito M,Yonetani Tdoi
10.1021/bi00281a019subject
Has Abstractpub_date
1983-06-07 00:00:00pages
2904-7issue
12eissn
0006-2960issn
1520-4995journal_volume
22pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Through the application of a unique method for isolating plasma membranes, it was possible to specifically iodinate cytoplasm-exposed plasma membrane proteins in vegetative cells of the cellular slime mold Dictyostelium discoideum. The original procedure [Chaney, L. K., & Jacobson, B. S. (1983) J. Biol. Chem. 258, 100...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00389a041
更新日期:1987-07-28 00:00:00
abstract::A procedure for universal 13C and/or 15N labeling of microbial peptides which are produced by fermentation in complex media and its application to two food-preserving bacteriocins from lactic acid bacteria are described. Isotopic enrichment of nisin A (from Lactococcus lactis) and of leucocin A (from Leuconostoc gelid...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00052a039
更新日期:1993-01-12 00:00:00
abstract::In vitro transcription by T7 RNA polymerase was used to prepare 32 different mutations in the 21 nucleotides that participate in the 9 tertiary base pairs or triples of yeast tRNAPhe. The mutations were designed either to disrupt the tertiary interaction or to change the sequence without disrupting the structure by tr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00462a014
更新日期:1990-03-13 00:00:00
abstract::Nucleic acid aptamers are rapidly gaining prominence as diagnostic tools, targeting reagents, and potential therapeutics. To extend the use of aptamers into the biochemical analysis of protein interactions on the surface of live cells, we converted an enzymatically generated RNA aptamer into a photo-cross-linkable aff...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi8004208
更新日期:2008-11-18 00:00:00
abstract::Esterification of retinol (vitamin A alcohol) with long-chain fatty acids by lecithin-retinol acyltransferase (LRAT) is an important step in both the absorption and storage of vitamin A. Retinol in cells is bound by either cellular retinol binding protein (CRBP), present in most tissues including liver, or cellular re...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00144a014
更新日期:1992-07-28 00:00:00
abstract::The effects of nonylphenol and 3,5-dibutyl-4-hydroxytoluene (BHT) on the activity of the (Ca2(+)-Mg2+)-ATPase of skeletal muscle sarcoplasmic reticulum have been studied. At high concentrations, both inhibit the ATPase activity of the ATPase either in native lipid or in bilayers of dioleoylphosphatidylcholine but, at ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00464a028
更新日期:1990-03-27 00:00:00
abstract::Channelrhodopsins (ChRs) from green flagellate algae function as light-gated ion channels when expressed heterologously in mammalian cells. Considerable interest has focused on understanding the molecular mechanisms of ChRs to bioengineer their properties for specific optogenetic applications such as elucidating the f...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi501243y
更新日期:2015-01-20 00:00:00
abstract::We have described earlier a chromatin-bound protease with unique specificity for histone H2A [Eickbush, T. H., Watson, D. K., & Moudrianakis, E. N. (1976) Cell (Cambridge, Mass.) 9, 785--792]. In the present study, we explore the nature of interactions that form and stabilize the enzyme-chromatin system by using the a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00531a008
更新日期:1982-01-19 00:00:00
abstract::DnaB helicase is responsible for unwinding duplex DNA during chromosomal DNA replication and is an essential component of the DNA replication apparatus in Escherichia coli. We have analyzed the mechanism of binding of single-stranded DNA (ssDNA) by the DnaB x DnaC complex and DnaB helicase. Binding of ssDNA to DnaB he...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060118d
更新日期:2006-09-26 00:00:00
abstract::The thermotropic behavior of aqueous dispersions of palmitoylsphingomyelin-cholesterol and lignoceryl-sphingomyelin-cholesterol mixtures has been examined by high-sensitivity differential scanning calorimetry. When less than 25 mol % cholesterol is mixed with either sphingomyelin, the calorimetric endotherm is compose...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00577a042
更新日期:1979-05-15 00:00:00
abstract::RalA and RalB are members of the Ras family of small G proteins and are activated downstream of Ras via RalGEFs. The RalGEF-Ral axis represents one of the major effector pathways controlled by Ras and as such is an important pharmacological target. RalA and RalB are approximately 80% identical at the amino acid level;...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi501530u
更新日期:2015-02-17 00:00:00
abstract::Phototropin, a blue-light photoreceptor in plants, has two FMN-binding domains named LOV1 and LOV2. We previously observed temperature-dependent FTIR spectral changes in the C=O stretching region (amide-I vibrational region of the peptide backbone) for the LOV2 domain of Adiantum phytochrome3 (phy3-LOV2), suggesting p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061837v
更新日期:2006-12-26 00:00:00
abstract::Rad51 protein, a eukaryotic homologue of RecA protein, forms a filamentous complex with DNA and catalyzes homologous recombination. We have analyzed the structure of Xenopus Rad51 protein (XRad51.1) in solution by small-angle neutron scattering (SANS). The measurements showed that XRad51.1 forms a helical filament ind...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971000n
更新日期:1997-11-04 00:00:00
abstract::Atomic resolution ( journal_title:Biochemistry pub_type: 杂志文章 doi:10.1021/bi0517133 更新日期:2006-02-21 00:00:00
abstract::alpha-Glucosidase (EC 3.2.1.20) was purified to homogeneity from logarithmically growing cells of Saccharomyces carlsbergensis. The purification involved the following steps: (a) ammonium sulfate fractionation; (b) Sephadex G-100 chromatography; (c) DEAE-cellulose chromatography; and (d) hydroxylapatite chromatography...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00615a011
更新日期:1978-10-31 00:00:00
abstract::While two-component systems (TCSs), composed of a sensor histidine kinase (HK) and a response regulator, are the main signaling pathways in bacteria, global TCS activity remains poorly described. Here, we report the kinetic parameters of the HK autophosphorylation reaction using previously uncharacterized γ-phosphate-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00485
更新日期:2018-07-24 00:00:00
abstract::The presence of a N-(2-deoxy-D-erythro pentofuranosyl)urea (henceforth referred to as deoxyribosylurea) residue, ring fragmentation product of a thymine, in a frameshift situation in the sequence 5'd(AGGACCACG).d(CGTGGurTCCT) has been studied by 1H and 31P nuclear magnetic resonance and molecular dynamics. At equilibr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971202j
更新日期:1998-01-27 00:00:00
abstract::Primase is an essential DNA replication enzyme in Escherichia coli and responsible for primer synthesis during lagging strand DNA replication. Although the interaction of primase with single-stranded DNA plays an important role in primer RNA and Okazaki fragment synthesis, the mechanism of DNA binding and site selecti...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026711m
更新日期:2002-12-17 00:00:00
abstract::Recombinant human ferrochelatase has been expressed in Escherichia coli and purified to homogeneity. Metal analyses revealed approximately 2 mol of non-heme Fe per mol of the purified enzyme (M(r) = 40,000). The UV-visible absorption spectrum of the purified enzyme consists of a protein absorption at 278 nm (epsilon a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00168a003
更新日期:1994-01-18 00:00:00
abstract::The membrane interaction properties of two single-residue variants, R6W and L5S, of the 17-amino acid neuropeptide dynorphin A (DynA) were studied by circular dichroism (CD) and nuclear magnetic resonance (NMR) spectroscopy. Corresponding gene mutations have recently been discovered in humans and causatively linked to...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi4004205
更新日期:2013-06-18 00:00:00
abstract::Two enantiomeric analogues of farnesyl pyrophosphate (1) were tested as inhibitors and anomalous substrates of trichodiene synthase, which catalyzes the cyclization of trans,trans-farnesyl pyrophosphate (1) to the sesquiterpene hydrocarbon trichodiene (2). The reaction has been shown to involve preliminary isomerizati...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00475a011
更新日期:1990-06-12 00:00:00
abstract::The active site structures of human Q31 granzyme A, murine granzymes (A, B, C, D, E, and F), and human granzymes (A, B, and 3) isolated from cytotoxic T lymphocytes (CTL) were studied with peptide thioester substrates, peptide chloromethyl ketone, and isocoumarin inhibitors. Human Q31, murine, and human granzyme A hyd...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00222a027
更新日期:1991-02-26 00:00:00
abstract::Prenyltransferase (EC 2.5.1.1) has been obtained from chicken liver in a stable crystalline form. The enzyme has been shown to be homogeneous by polyacrylamide gel electrophoresis at pH 8.4, and by electrophoresis in sodium dodecyl sulfate containing gels. Electrofocusing of the crystalline enzyme results in a single ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00672a009
更新日期:1975-01-14 00:00:00
abstract::Paranemic crossover DNA (PX-DNA) is a four-stranded multicrossover structure that has been implicated in recombination-independent recognition of homology. Although existing evidence has suggested that PX is the DNA motif in homologous pairing (HP), this conclusion remains ambiguous. Further investigation is needed bu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00057
更新日期:2019-03-12 00:00:00
abstract::1H NMR spectral editing techniques can select the distinct signals of lactate, pyruvate, beta-hydroxybutyrate, and acetoacetate and provide a unique way to monitor the biochemical processes in vivo. These metabolite levels reflect the near-equilibrium dehydrogenase activity and therefore the cellular redox state. The ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00160a029
更新日期:1992-11-17 00:00:00
abstract::Folding models suggest that the highly conserved histidine 217 of the cytochrome b subunit from the cytochrome bc1 complex is close to the quinone reductase (Qi) site. This histidine (bH217) in the cytochrome b polypeptide of the photosynthetic bacterium Rhodobacter capsulatus has been replaced with three other residu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00169a014
更新日期:1994-01-25 00:00:00
abstract::Herpesvirus saimiri encodes a tyrosine kinase interacting protein (Tip) that binds to T-cell-specific tyrosine kinase Lck via multiple sequence motifs and controls its activity. The regulation of Lck by Tip represents a key mechanism in the transformation of human T-lymphocytes during herpesviral infection. In this st...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0485068
更新日期:2004-11-30 00:00:00
abstract::Reporter gene transactivation by human p53 is inhibited in budding yeast lacking the TRR1 gene encoding thioredoxin reductase. To investigate the role of thioredoxin in controlling p53 activity, the level of reporter gene transactivation by p53 was determined in yeast lacking the TRX1 and TRX2 genes encoding cytosolic...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900757q
更新日期:2009-09-29 00:00:00
abstract::DNA sequences containing homopurine d(G1-3A)n tracts are known to be capable of adopting non-B-DNA conformations. The structural polymorphism of these sequences is a direct consequence of the structural properties of the homopurine d(G1-3A)n tracts. Depending on the conditions, d(GA)n DNA sequences can form antiparall...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961020+
更新日期:1996-10-08 00:00:00
abstract::The voltage-gated potassium channel, K(v)1.3, is a novel target for development of immunosuppressants. Using a functional (86)Rb(+) efflux assay, a new class of high-affinity K(v)1.3 inhibitors has been identified. The initial active in this series, 4-phenyl-4-[3-(2-methoxyphenyl)-3-oxo-2-azaprop-1-yl]cyclohexanone (P...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi025722c
更新日期:2002-06-18 00:00:00