当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Effect of thioredoxin deletion and p53 cysteine replacement on human p53 activity in wild-type and thioredoxin reductase null yeast.

Effect of thioredoxin deletion and p53 cysteine replacement on human p53 activity in wild-type and thioredoxin reductase null yeast.

Abstract：

:Reporter gene transactivation by human p53 is inhibited in budding yeast lacking the TRR1 gene encoding thioredoxin reductase. To investigate the role of thioredoxin in controlling p53 activity, the level of reporter gene transactivation by p53 was determined in yeast lacking the TRX1 and TRX2 genes encoding cytosolic thioredoxin. Surprisingly, p53 activity was unimpaired in yeast lacking thioredoxin. Subsequent analyses showed that thioredoxin deletion suppressed the inhibitory effect of thioredoxin reductase deletion, suggesting that accumulation of oxidized thioredoxin in mutant yeast was necessary for p53 inhibition. Purified human thioredoxin and p53 interacted in vitro (Kd = 0.9 microM thioredoxin). To test the idea that dithio-disulfide exchange reactions between p53 and thioredoxin were responsible for p53 inhibition in mutant yeast, each p53 cysteine was changed to serine, and the effect of the substitution on p53 activity in TRR1 and Deltatrr1 yeast was determined. Substitutions at Zn-coordinating cysteines C176, C238, or C242 resulted in p53 inactivation. Unexpectedly, substitution at cysteine C275 also inactivated p53, which was the first evidence for a non-zinc-coordinating cysteine being essential for p53 function. Cysteine substitutions at six positions (C124, C135, C141, C182, C229, and C277) neither inactivated p53 nor relieved the requirement for thioredoxin reductase. Furthermore, no tested combination of these six cysteine substitutions relieved thioredoxin reductase dependence. The results suggested that p53 dependence on thioredoxin reductase either was indirect, perhaps mediated by an upstream activator of p53, or was due to oxidation of one or more of the four essential cysteines.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Stoner CS,Pearson GD,Koç A,Merwin JR,Lopez NI,Merrill GF

doi

10.1021/bi900757q

subject

Has Abstract

pub_date

2009-09-29 00:00:00

pages

9156-69

issue

38

eissn

0006-2960

issn

1520-4995

journal_volume

48

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Fe protein-independent substrate reduction by nitrogenase MoFe protein variants.

    abstract：:The reduction of substrates catalyzed by nitrogenase normally requires nucleotide-dependent Fe protein delivery of electrons to the MoFe protein, which contains the active site FeMo cofactor. Here, it is reported that independent substitution of three amino acids (β-98(Tyr→His), α-64(Tyr→His), and β-99(Phe→His)) locat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00140

    authors： Danyal K,Rasmussen AJ,Keable SM,Inglet BS,Shaw S,Zadvornyy OA,Duval S,Dean DR,Raugei S,Peters JW,Seefeldt LC

    更新日期：2015-04-21 00:00:00

  • Crystal structure of human fibrinogen.

    abstract：:A crystal structure of human fibrinogen has been determined at approximately 3.3 A resolution. The protein was purified from human blood plasma, first by a cold ethanol precipitation procedure and then by stepwise chromatography on DEAE-cellulose. A product was obtained that was homogeneous on SDS-polyacrylamide gels....

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi802205g

    authors： Kollman JM,Pandi L,Sawaya MR,Riley M,Doolittle RF

    更新日期：2009-05-12 00:00:00

  • A novel delta(3),delta(2)-enoyl-CoA isomerase involved in the biosynthesis of the cyclohexanecarboxylic acid-derived moiety of the polyketide ansatrienin A.

    abstract：:The side chain of the antifungal polyketide ansatrienin A produced by Streptomyces collinus contains a cyclohexanecarboxylic acid (CHC) derived moiety. This CHC in the coenzyme A activated form (CHC-CoA) is derived from shikimic acid via a pathway in which the penultimate step is the isomerization of 2-cyclohexenylcar...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0005714

    authors： Patton SM,Cropp TA,Reynolds KA

    更新日期：2000-06-27 00:00:00

  • tRNA glycylation system from Thermus thermophilus. tRNAGly identity and functional interrelation with the glycylation systems from other phylae.

    abstract：:The systems of tRNA glycylation belong to the most complex aminoacylation systems since neither the oligomeric structure of glycyl-tRNA synthetases (GlyRS) nor the discriminator bases in tRNAGly are conserved in the phylae. To better understand the structure-function relationship in glycylation systems of various orig...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi991392t

    authors： Mazauric MH,Roy H,Kern D

    更新日期：1999-10-05 00:00:00

  • Conversion of aminodeoxychorismate synthase into anthranilate synthase with Janus mutations: mechanism of pyruvate elimination catalyzed by chorismate enzymes.

    abstract：:The central importance of chorismate enzymes in bacteria, fungi, parasites, and plants combined with their absence in mammals makes them attractive targets for antimicrobials and herbicides. Two of these enzymes, anthranilate synthase (AS) and aminodeoxychorismate synthase (ADCS), are structurally and mechanistically ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00013

    authors： Culbertson JE,Chung Dh,Ziebart KT,Espiritu E,Toney MD

    更新日期：2015-04-14 00:00:00

  • Minor-groove recognition of double-stranded RNA by the double-stranded RNA-binding domain from the RNA-activated protein kinase PKR.

    abstract：:The human double-stranded RNA- (dsRNA) activated protein kinase (PKR) has a dsRNA-binding domain (dsRBD) that contains two tandem copies of the dsRNA-binding motif (dsRBM). The minimal-length polypeptide required to bind dsRNA contains both dsRBMs, as determined by mobility-shift and filter-binding assays. Mobility-sh...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9607259

    authors： Bevilacqua PC,Cech TR

    更新日期：1996-08-06 00:00:00

  • Avian synaptopodin 2 (fesselin) stabilizes myosin filaments and actomyosin in the presence of ATP.

    abstract：:Smooth muscle cells maintain filaments of actin and myosin in the presence of ATP, although dephosphorylated myosin filaments and actin-myosin interactions are unstable under those conditions in vitro. Several proteins that stabilize myosin filaments and that stabilize actin-myosin interactions have been identified. F...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi401013g

    authors： Kingsbury NL,Renegar RH,Chalovich JM

    更新日期：2013-10-29 00:00:00

  • Cooperative binding of R17 coat protein to RNA.

    abstract：:The binding of the R17 coat protein to synthetic RNAs containing one or two coat protein binding sites was characterized by using nitrocellulose filter and gel-retention assays. RNAs with two available sites bound coat protein in a cooperative manner, resulting in a higher affinity and reduced sensitivity to pH, ionic...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00502a006

    authors： Witherell GW,Wu HN,Uhlenbeck OC

    更新日期：1990-12-18 00:00:00

  • Kinetic, Raman, NMR, and site-directed mutagenesis studies of the Pseudomonas sp. strain CBS3 4-hydroxybenzoyl-CoA thioesterase active site.

    abstract：:4-Hydroxybenzoyl-coenzyme A (4-HBA-CoA) thioesterase catalyzes the hydrolysis of 4-HBA-CoA to 4-hydroxybenzoate and CoA. X-ray crystallographic analysis of the liganded enzyme has shown that the benzoyl thioester and pantetheine moieties of the substrate ligand are bound in a narrow crevice while the nucleotide moiety...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0262303

    authors： Zhuang Z,Song F,Zhang W,Taylor K,Archambault A,Dunaway-Mariano D,Dong J,Carey PR

    更新日期：2002-09-17 00:00:00

  • Modified-peptide inhibitors of amyloid beta-peptide polymerization.

    abstract：:Cellular toxicity resulting from nucleation-dependent polymerization of amyloid beta-peptide (Abeta) is considered to be a major and possibly the primary component of Alzheimer's disease (AD). Inhibition of Abeta polymerization has thus been identified as a target for the development of therapeutic agents for the trea...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi982824n

    authors： Findeis MA,Musso GM,Arico-Muendel CC,Benjamin HW,Hundal AM,Lee JJ,Chin J,Kelley M,Wakefield J,Hayward NJ,Molineaux SM

    更新日期：1999-05-25 00:00:00

  • Enthalpic destabilization of a mutant human lysozyme lacking a disulfide bridge between cysteine-77 and cysteine-95.

    abstract：:To understand the role of disulfide bridges in protein stability, the thermodynamic changes in the denaturation of two mutant human lysozymes lacking a disulfide bridge between Cys-77 and Cys-95 (C77A and C77/95A) were analyzed using differential scanning calorimetry (DSC). At pH 3.0 and 57 degrees C, the stabilities ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00150a028

    authors： Kuroki R,Inaka K,Taniyama Y,Kidokoro S,Matsushima M,Kikuchi M,Yutani K

    更新日期：1992-09-08 00:00:00

  • Catalytic properties of hairpin ribozymes derived from Chicory yellow mottle virus and arabis mosaic virus satellite RNAs.

    abstract：:Regions of the negative strands of the satellite RNAs of chicory yellow mottle virus (sCYMV1) and arabis mosaic virus (sArMV) have similarity in sequence and predicted secondary structure compared to the tobacco ringspot virus satellite RNA (sTRSV) hairpin ribozyme, suggesting that they may also be catalytic RNAs of a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00048a024

    authors： DeYoung M,Siwkowski AM,Lian Y,Hampel A

    更新日期：1995-12-05 00:00:00

  • Cobalt-cytochrome c. I. Preparation, properties, and enzymic activity.

    abstract：:An improved procedure for the preparation of cobalt-cytochrome c has been developed. Various factors influencing the cobalt insertion process are discussed. The optical spectra of cobalt-cytochrome c suggest a six-coordinated species. The spectral shifts occurring with oxidation-reduction are compared with those obser...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00687a003

    authors： Dickinson LC,Chien JC

    更新日期：1975-08-12 00:00:00

  • Mechanism of irreversible inhibition of O2 evolution in photosystem II by Tris(hydroxymethyl)aminomethane.

    abstract：:The dark reaction of tris(hydroxymethyl)aminomethane (Tris) with the O2-evolving center of photosystem II (PSII) in the S1 state causes irreversible inhibition of O2 evolution. Similar inhibition is observed for several other amines: NH3, CH3NH2, (CH3)2NH, ethanolamine, and 2-amino-2-ethyl-1,3-propanediol. In PSII mem...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00246a003

    authors： Rickert KW,Sears J,Beck WF,Brudvig GW

    更新日期：1991-08-13 00:00:00

  • Conformational changes and subunit communication in tryptophan synthase: effect of substrates and substrate analogs.

    abstract：:The transmission of regulatory signals between the alpha- and beta-subunits of the tryptophan synthase alpha 2 beta 2 complex from Salmonella typhimurium has been investigated by monitoring the luminescence properties of the enzyme in the presence and in the absence of the alpha-subunit ligand DL-alpha-glycerol 3-phos...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00148a014

    authors： Strambini GB,Cioni P,Peracchi A,Mozzarelli A

    更新日期：1992-08-25 00:00:00

  • Structure of an active water molecule in the water-oxidizing complex of photosystem II as studied by FTIR spectroscopy.

    abstract：:The vibrations of a water molecule in the water-oxidizing complex (WOC) of photosystem II were detected for the first time using Fourier transform infrared (FTIR) spectroscopy. In a flash-induced FTIR difference spectrum upon the S(1)-to-S(2) transition, a pair of positive and negative bands was observed at 3618 and 3...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi001040i

    authors： Noguchi T,Sugiura M

    更新日期：2000-09-12 00:00:00

  • 4-Azido-2-nitrophenyl phosphate, a new photoaffinity derivative of inorganic phosphate. Study of its interaction with the inorganic phosphate binding site of beef heart mitochondrial adenosine triphosphatase.

    abstract：:4-Azido-2-nitrophenyl phosphate (ANPP) was synthesized and characterized. ANPP, unlabeled or labeled by 32P, was used as a photoreactive analogue of Pi to study the Pi binding site(s) in isolated F1-ATPase and inside-out particles from beef heart mitochondria. In the dark, the phosphate bond of ANPP was cleaved by alk...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00561a013

    authors： Lauquin G,Pougeois R,Vignais PV

    更新日期：1980-09-30 00:00:00

  • Photoaffinity labeling of the alpha 1-adrenergic receptor using an 125I-labeled aryl azide analogue of prazosin.

    abstract：:alpha 1-Adrenergic receptor probes, which can be radioiodinated to yield high specific activity radioligands, have been synthesized and characterized. 2-[4-(4-Amino-benzoyl)piperazin-1-yl]-4-amino-6,7-dimethoxyquin azoline (CP63,155), an arylamine analogue of the selective alpha 1-adrenergic antagonist prazosin, and i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00311a031

    authors： Seidman CE,Hess HJ,Homcy CJ,Graham RM

    更新日期：1984-07-31 00:00:00

  • Increased thermal stability of proteins in the presence of naturally occurring osmolytes.

    abstract：:Organisms and cellular systems which have adapted to stresses such as high temperature, desiccation, and urea-concentrating environments have responded by concentrating particular organic solutes known as osmolytes. These osmolytes are believed to confer protection to enzyme and other macromolecular systems against su...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00138a006

    authors： Santoro MM,Liu Y,Khan SM,Hou LX,Bolen DW

    更新日期：1992-06-16 00:00:00

  • Isolation and characterization of neutralizing single-chain antibodies against Xenopus mitogen-activated protein kinase kinase from phage display libraries.

    abstract：:MAP kinase kinase (MAPKK) is a dual specificity protein kinase that phosphorylates and activates MAP kinase in vivo. In this study, four mouse monoclonal single-chain Fv (scFv) antibodies (Y1-6, Y1-7, Y3-6, and Y3-11) that can specifically bind to Xenopus MAPKK were isolated from combinatorial scFv-displaying phage li...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi960956f

    authors： Kosako H,Akamatsu Y,Tsurushita N,Lee KK,Gotoh Y,Nishida E

    更新日期：1996-10-08 00:00:00

  • Solid state 15N NMR evidence for a complex Schiff base counterion in the visual G-protein-coupled receptor rhodopsin.

    abstract：:Using the baculovirus/Sf9 cell expression system, we have incorporated 99% 15N-enriched [alpha,epsilon-15N2]-L-lysine into the rod visual pigment rhodopsin. We have subsequently investigated the protonated Schiff base (pSB) linkage in the [alpha, epsilon-15N2]Lys-rhodopsin with cross-polarization magic angle spinning ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9830157

    authors： Creemers AF,Klaassen CH,Bovee-Geurts PH,Kelle R,Kragl U,Raap J,de Grip WJ,Lugtenburg J,de Groot HJ

    更新日期：1999-06-01 00:00:00

  • Inhibition of protein synthesis in vesicular stomatitis virus infected Chinese hamster ovary cells: role of virus mRNA-ribonucleoprotein particle.

    abstract：:Although host protein synthesis is preferentially inhibited, there is a steady decline in the ability of Chinese hamster ovary (CHO) cells infected with vesicular stomatitis virus (VSV) to synthesize both host and viral proteins. We previously reported finding an mRNA-ribonucleoprotein particle (mRNP) that contained a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00306a014

    authors： Rosen CA,Siekierka J,Ennis HL,Cohen PS

    更新日期：1984-05-22 00:00:00

  • Crystallographic Structures of IlvN·Val/Ile Complexes: Conformational Selectivity for Feedback Inhibition of Aceto Hydroxy Acid Synthases.

    abstract：:Conformational factors that predicate selectivity for valine or isoleucine binding to IlvN leading to the regulation of aceto hydroxy acid synthase I (AHAS I) of Escherichia coli have been determined for the first time from high-resolution (1.9-2.43 Å) crystal structures of IlvN·Val and IlvN·Ile complexes. The valine ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b00050

    authors： Bansal A,Karanth NM,Demeler B,Schindelin H,Sarma SP

    更新日期：2019-04-16 00:00:00

  • A new pathway for transmembrane electron transfer in photosynthetic reaction centers of Rhodobacter sphaeroides not involving the excited special pair.

    abstract：:It is generally accepted that electron transfer in bacterial photosynthesis is driven by the first singlet excited state of a special pair of bacteriochlorophylls (P*). We have examined the first steps of electron transfer in a mutant of the Rhodobacter sphaeroides reaction center in which charge separation from P* is...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9703756

    authors： Van Brederode ME,Jones MR,Van Mourik F,Van Stokkum IH,Van Grondelle R

    更新日期：1997-06-10 00:00:00

  • Rapid laser flash photoaffinity labeling of binding sites for a noncompetitive inhibitor of the acetylcholine receptor.

    abstract：:Photoaffinity labeling of the nicotinic acetylcholine receptor from Torpedo marmorata electric tissue was performed in the presence of cholinergic effectors in the millisecond to second time range by a combination of a stopped-flow apparatus and a high-energy pulse laser. The label applied was [3H]triphenylmethylphosp...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00307a029

    authors： Muhn P,Fahr A,Hucho F

    更新日期：1984-06-05 00:00:00

  • Light-induced global conformational change of photoactive yellow protein in solution.

    abstract：:The light-induced global conformational change of photoactive yellow protein was directly observed by small-angle X-ray scattering (SAXS). The N-terminal 6, 15, or 23 amino acid residues were enzymatically truncated (T6, T15, or T23, respectively), and their near-UV intermediates were accumulated under continuous illu...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0264768

    authors： Imamoto Y,Kamikubo H,Harigai M,Shimizu N,Kataoka M

    更新日期：2002-11-19 00:00:00

  • Formation and rejoining of deoxyribonucleic acid double-strand breaks induced in isolated cell nuclei by antineoplastic intercalating agents.

    abstract：:The biochemical characteristics of the formation and disappearance of intercalator-induced DNA double-strand breaks (DSB) were studied in nuclei from mouse leukemia L1210 cells by using filter elution methodology [Bradley, M. O., & Kohn, K.W. (1979) Nucleic Acids Res. 7, 793-804]. The three intercalators used were 4'-...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00309a013

    authors： Pommier Y,Schwartz RE,Kohn KW,Zwelling LA

    更新日期：1984-07-03 00:00:00

  • Faithful initiation of ribosomal RNA transcription from cloned DNA by purified RNA polymerase I.

    abstract：:A faithful transcription system for ribosomal RNA genes has been developed by using components from the small free-living amoeba Acanthamoeba castellanii. The system utilizes protein-free recombinant DNA as a template and in addition requires a crude cell-free extract containing RNA polymerase I and a transcription in...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00313a025

    authors： Paule MR,Iida CT,Perna PJ,Harris GH,Brown Shimer SL,Kownin P

    更新日期：1984-08-28 00:00:00

  • von Willebrand factor antagonizes nitric oxide synthase to promote insulin resistance during hypoxia.

    abstract：:Hypoxic respiratory diseases or hypoxia exposures are frequently accompanied by glucose intolerance and impaired nitric oxide (NO) availability. However, the molecular mechanism responsible for impaired NO production and insulin resistance (IR) during hypoxia remains obscure. In this study, we investigated the possibl...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi401061e

    authors： Singh B,Biswas I,Garg I,Sugadev R,Singh AK,Dey S,Khan GA

    更新日期：2014-01-14 00:00:00

  • Product Release Mechanism Associated with Structural Changes in Monomeric l-Threonine 3-Dehydrogenase.

    abstract：:A short chain dehydrogenase like l-threonine 3-dehydrogenase (SDR-TDH) from metagenome data (mtTDH) was identified by database mining. Its enzymatic properties suggested that mtTDH has unique characteristics relative to other SDR-TDHs, including two mesophilic and thermophilic SDR-TDHs identified in this study. The ac...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b00832

    authors： Motoyama T,Nakano S,Yamamoto Y,Tokiwa H,Asano Y,Ito S

    更新日期：2017-10-31 00:00:00