Abstract:
:The systems of tRNA glycylation belong to the most complex aminoacylation systems since neither the oligomeric structure of glycyl-tRNA synthetases (GlyRS) nor the discriminator bases in tRNAGly are conserved in the phylae. To better understand the structure-function relationship in glycylation systems of various origins and the functional peculiarities related to their structural divergences, the elements in tRNA conferring its glycine identity in Thermus thermophilus were characterized and compared to those of other systems. Thermophilic identity is conferred by the G1-C72, C2-G71, G3-C70, and C50-G64 pairs together with the G10, U16, C35, and C36 single residues. In contrast to most other aminoacylation systems, the discriminator base is not directly involved in identity. Transplantation of these elements in tRNAAsp and tRNAPhe converts specificity toward glycine albeit conservation of nucleotide 73. Analysis of the functional interrelation of the identity elements shows coupling in synthetase recognition of the elements from anticodon and G10 whereas those from acceptor arm are recognized independently. Despite nondirect implication in identity, the discriminator base contributes cooperatively with C36 in specificity of glycylation. The link between the structural heterogeneity and the functional divergence of the glycylation systems and the phylogenic interrelation of these systems were approached by comparing the ability of GlyRSs of various phylae to glycylate heterologous tRNAGly. Dimeric GlyRSs from mammalian and archaebacteria acylate efficiently only eukaryotic and archaebacterial tRNAGly with a discriminatory A73, whereas tetrameric Escherichia coli GlyRS acylates only eubacterial tRNAGly with a discriminatory U73. In contrast, dimeric yeast GlyRS acylates efficiently both eukaryotic and archaebacterial tRNAGly as well as peculiar prokaryotic isoacceptors. Species specificity is lost with the dimeric GlyRS from Thermus thermophilus that acylates efficiently eubacterial, archaebacterial, and eukaryotic tRNAGly. These features are discussed in the context of the evolution of the glycylation systems and the phylogenic interrelation of the organisms.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Mazauric MH,Roy H,Kern Ddoi
10.1021/bi991392tsubject
Has Abstractpub_date
1999-10-05 00:00:00pages
13094-105issue
40eissn
0006-2960issn
1520-4995pii
bi991392tjournal_volume
38pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::FtmPT1 is a fungal indole prenyltransferase that catalyzes the reaction of tryptophan derivatives with dimethylallyl pyrophosphate to form various biologically active compounds. Herein, we describe detailed studies of FtmPT1 catalysis involving dimethylallyl pyrophosphate and Brevianamide F following the native pathwa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500747z
更新日期:2014-09-30 00:00:00
abstract::The formation of closed icosahedral capsids from a single species of coat protein subunit requires that the subunits assume different conformations at different lattice positions. In the double-stranded DNA bacteriophage P22, formation of correctly dimensioned capsids is mediated by interaction between coat protein su...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00475a030
更新日期:1990-06-12 00:00:00
abstract::The phosphoramidate of CCdAp and puromycin (CCdApPuro) is a potent inhibitor of ribosomal peptidyl transferase, as assayed by the fragment reaction. Inhibition is competitive at the ribosomal A-site. CCdApPuro protects P-site-associated bases in the peptidyl transferase loop region of 23S rRNA from carbodiimide modifi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00002a001
更新日期:1995-01-17 00:00:00
abstract::An NMR study has been made of porcine pancreatic phospholipase A2 (PLA) in three environments: free in solution, in a binary complex with dodecylphosphocholine micelles, and in a ternary complex with a micelle and the substrate-like inhibitor (R)-1-octyl-2-(N-dodecanoylamino)-2-deoxyglycero-3-phosph oglycol. 1H and 15...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00156a023
更新日期:1992-10-20 00:00:00
abstract::The fungal metabolite gliotoxin has a redox-active disulfide bridge spanning carbons 3 and 6 of a diketopiperazine (DKP) scaffold. The proposed DKP synthetase, GliP, from Aspergillus fumigatus Af293, is a three module (A1-T1-C1-A2-T2-C2-T3) 236 kDa protein that can be overproduced in soluble form in Escherichia coli. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061845b
更新日期:2006-12-19 00:00:00
abstract::We report that recombinant glia maturation factor (GMF), a 17-kDa brain protein, inhibits the activity of mitogen-activated protein (MAP) kinase in the test tube assay, in particular the ERK1/ERK2 isoforms. A preliminary phosphorylation of GMF by protein kinase A (PKA) dramatically increases its inhibitory effect by o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi960034c
更新日期:1996-05-21 00:00:00
abstract::The effects of NH3 on the oxygen evolving enzyme have been investigated with EPR and steady-state O2 evolution. The following results were obtained. At low light intensity O2 evolution occurs in all centers even though ammonia is bound. This binding occurs in the S2 state and results in a modification of the multiline...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00453a003
更新日期:1990-01-09 00:00:00
abstract::Escherichia coli mannitol specific EII in membrane vesicles can be inhibited by the action of the oxidizable substrate-reduced phenazine methosulfate (PMS) in a manner similar to E. coli enzyme IIGlc [Robillard, G. T., & Konings, W. (1981) Biochemistry 20, 5025-5032]. The fact that reduced PMS and various oxidizing ag...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00297a006
更新日期:1984-01-17 00:00:00
abstract::To be fully active and participate in the metabolism of phosphorylated nucleotides, most nucleoside diphosphate kinases (NDPKs) have to assemble into stable hexamers. Here we studied the role played by six intersubunit salt bridges R80-D93 in the stability of NDPK from the pathogen Mycobacterium tuberculosis ( Mt). Mu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b01308
更新日期:2019-03-12 00:00:00
abstract::The quaternary structures of Escherichia coli DNA-dependent RNA polymerase holenzyme (alpha 2 beta beta' sigma) and core enzyme (alpha 2 beta beta') have been investigated by chemical cross-linking with a cleavable bifunctional reagent, methyl 4-mercaptobutyrimidate, and noncleavable reagents, dimethyl suberimidate an...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00634a008
更新日期:1977-07-26 00:00:00
abstract::To probe attitudinal features of the Ca(2+)-deficient site (site I) in the Ca2+ switch of cardiac troponin C (cTnC), we have examined steady-state fluorescence emission and polarization of a Trp26 inserted in a recombinant cardiac TnC (cTnC3.W) and compared these with the properties of the Ca(2+)-competent site I in s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00002a022
更新日期:1995-01-17 00:00:00
abstract::Cytochrome P-450(M-1) [P-450(M-1)] is specifically expressed in adult male rat liver [Yoshioka, H., Morohashi, K., Sogawa, K., Miyata, T., Kawajiri, K., Hirose, T., Inayama, S., Fujii-Kuriyama, Y., & Omura, T. (1987) J. Biol. Chem. 262, 1706-1711]. Isolation and analysis of the gene for P-450(M-1) revealed that the co...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00399a039
更新日期:1987-12-15 00:00:00
abstract::In eukaryotic cells, chromatin is classified into euchromatin, which is active in transcription, and heterochromatin that silences transcription. Histones in these two domains contain distinct modifications. Chromatin assembly factor 1 (CAF-1) is a highly conserved protein that functions in DNA replication, DNA repair...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0521083
更新日期:2006-03-07 00:00:00
abstract::The structure and backbone dynamics of an extended second transmembrane segment (TM2e) of the human neuronal glycine receptor alpha(1) subunit in sodium dodecyl sulfate micelles were studied by (1)H and (15)N solution-state NMR. The 28-amino acid segment contained the consensus TM2 domain plus part of the linker betwe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026767g
更新日期:2003-04-08 00:00:00
abstract::Pyridoxal 5'-triphospho-5'-adenosine (AP3-PL), the affinity labeling reagent specific for lysine residues in the nucleotide-binding site of several enzymes [Tagaya, M., & Fukui, T. (1986) Biochemistry 25, 2958-2964; Yagami, T., Tagaya, M., & Fukui, T. (1988) FEBS Lett. 229, 261-264], was used to identify the ATP-bindi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00503a016
更新日期:1990-12-25 00:00:00
abstract::cGMP is the natural activator of the cyclic nucleotide-gated channel originally isolated from rod photoreceptors but now known to be expressed in a wide variety of neural and non-neural cells. To identify antagonists of cGMP action and to better understand the interaction between cGMP and the channel protein, experime...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961763v
更新日期:1996-12-24 00:00:00
abstract::We have purified the junctional acetylcholine receptor from normal rat skeletal muscle and compared its structure with that of the extrajunctional receptor from denervated muscle. The two receptors from leg muscle were distinguished by isoelectric focusing and by reaction with sera from patients with myasthenia gravis...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00582a028
更新日期:1979-07-24 00:00:00
abstract::The individual amide proton exchange rates in Tendamistat at pH 3.0 and 50 degrees C were measured by using two-dimensional 1H nuclear magnetic resonance. Overall, it was found that the distribution of exchange rates along the sequence is dominated by the interstrand hydrogen bonds of the beta-sheet structures. The sl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00394a030
更新日期:1987-10-06 00:00:00
abstract::The Hsp70-class molecular chaperone HscA interacts specifically with a conserved (99)LPPVK(103) motif of the iron-sulfur cluster scaffold protein IscU. We used a cellulose-bound peptide array to perform single-site saturation substitution of peptide residues corresponding to Glu(98)-Ile(104) of IscU to determine posit...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0606187
更新日期:2006-07-04 00:00:00
abstract::The 1H resonances of the high-potential [4Fe-4S]2+ ferredoxin from Chromatium vinosum have been assigned through conventional sequential methodology applied to 2D NMR spectra. Almost 80% of the residues were identified using standard 2D COSY, HOHAHA, and NOESY pulse sequences. These residues correspond to four segment...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00139a029
更新日期:1992-06-23 00:00:00
abstract::Through a ligand-directed tosyl (LDT) chemistry strategy using the synthetic acetogenin ligand AL1, we succeeded in the pinpoint alkynylation (-C≡CH) of Asp160 in the 49 kDa subunit of bovine complex I, which may be located in the inner part of the putative quinone binding cavity of the enzyme [Masuya, T., et al. (201...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi501342w
更新日期:2014-12-16 00:00:00
abstract::The 2-thiocytidine residue at position 32 of tRNA1Arg from Escherichia coli was modified specifically with three photoaffinity reagents of different lengths, and the corresponding N-acetylarginyl-tRNA1Arg derivatives were cross-linked to the P site of E. coli 70S ribosomes by irradiation. Covalent attachment was depen...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00339a011
更新日期:1985-08-27 00:00:00
abstract::In the minor fraction of HDL3 containing alpha-tocopherol (alphaTocOH), selective one-electron oxidation of Trp and Tyr residues of apolipoproteins A-I and A-II by *Br2- radical-anions produces the corresponding semioxidized species, TyrO* and *Trp. Repair of TyrO* by endogenous alphaTocOH generates the alpha-tocopher...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701419d
更新日期:2007-12-11 00:00:00
abstract::Positively charged cyclic hexapeptide Phe-Arg-Cys-Arg-Cys-Phe-CONH2 (FRCRCFa) represents a group of conformationally constrained peptides that block the cardiac sarcolemma Na(+)-Ca2+ exchanger [Khananshvili et al. (1995b) J. Biol. Chem. 270, 16182-16188]. Here, we study the kinetic mechanisms of FRCRCFa-induced inhibi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961099i
更新日期:1996-12-10 00:00:00
abstract::Recently, it was demonstrated that bifunctional catalase-peroxidases (KatGs) are found not only in archaea and bacteria but also in lower eukaryotes. Structural studies and preliminary biochemical data of the secreted KatG from the rice pathogen Magnaporthe grisea (MagKatG2) suggested both similar and novel features w...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00831
更新日期:2015-09-08 00:00:00
abstract::To clarify the function of the hydrophilic carboxyl-terminal tail of human erythrocyte membrane band 3 protein (HEM-B3), we purified two peptides, C1 (Ala893-Val911) and KS4 (Gly647-Arg656), from human erythrocyte band 3 protein preparations. Purified C1 peptides at concentrations from 5 to 80 microM were incubated wi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035281c
更新日期:2004-02-17 00:00:00
abstract::We have examined the effects on redox kinetics of changing the reduction potential of the mu-oxo-bridged binuclear iron center in octameric hemerythrin (Hr) from Phascolopsis gouldii. The opportunity to examine such effects is provided by the availability of mu-sulfidomethemerythrin (mu-S2-metHr), whose [Fe(III),Fe(II...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00400a033
更新日期:1987-12-29 00:00:00
abstract::Eukaryotic initiation factor 5 (eIF5) plays multiple roles in translation initiation. Its N-terminal domain functions as a GTPase-activator protein (GAP) for GTP bound to eIF2, while its C-terminal region nucleates the interactions between multiple translation factors, including eIF1, which acts to inhibit GTP hydroly...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi052387u
更新日期:2006-04-11 00:00:00
abstract::The kinetics of tert-butyl isocyanide binding to the heme protein horseradish peroxidase (HRP) at 22 degrees C was examined on all time scales, from minutes to picoseconds, in aqueous borate buffer at pH 9.08. Unlike myoglobin (Mb) or hemoglobin, HRP shows two bimolecular ligand binding processes. For comparison, bind...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9518149
更新日期:1996-02-06 00:00:00
abstract::Two short amino acid motifs, WXGDXNXR and PXWCDRXL, define a large family of inositol polyphosphate 5-phosphatases. We tested the importance of seven of these conserved amino acids to substrate binding and catalysis by mutating each to alanine in the platelet 75 kDa inositol polyphosphate 5-phosphatase II (5-phosphata...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9602627
更新日期:1996-06-18 00:00:00