Gene structure of cytochrome P-450(M-1) specifically expressed in male rat liver.

abstract：:CD4 is expressed on the surface of specific leukocytes where it plays a key role in the activation of immunostimulatory T-cells and acts as a primary receptor for HIV-1 entry. CD4 has four ecto-domains (D1-D4) of which D1, D2, and D4 contain disulfide bonds. Although disulfide bonds commonly serve structural or cataly...

journal_title：Biochemistry

authors： Owen GR,Channell JA,Forsyth VT,Haertlein M,Mitchell EP,Capovilla A,Papathanasopoulos M,Cerutti NM

更新日期：2016-04-19 00:00:00

abstract：:Betaine-homocysteine S-methyltransferase (BHMT) is a zinc-dependent enzyme that catalyzes the transfer of a methyl group from glycine betaine (Bet) to homocysteine (Hcy) to form dimethylglycine (DMG) and methionine (Met). Previous studies in other laboratories have indicated that catalysis proceeds through the formati...

journal_title：Biochemistry

authors： Castro C,Gratson AA,Evans JC,Jiracek J,Collinsová M,Ludwig ML,Garrow TA

更新日期：2004-05-11 00:00:00

abstract：:Inherently curved DNA segments, associated with short runs of adenines, have been identified in many gene regulatory regions, yet their physiological significance remains unknown. The observations reported in this study indicate that intrinsically bent nucleic acid fragments are characterized by substantially attenuat...

journal_title：Biochemistry

authors： Shatzky-Schwartz M,Hiller Y,Reich Z,Ghirlando R,Weinberger S,Minsky A

更新日期：1992-03-03 00:00:00

abstract：:A high degree of selectivity toward the target site of the pest organism is a desirable attribute for new safer agrochemicals. To assist in the design of novel herbicides, we determined the crystal structures of the herbicidal target enzyme 4-hydroxyphenylpyruvate dioxygenase (HPPD; EC 1.13.11.27) from the plant Arabi...

journal_title：Biochemistry

authors： Yang C,Pflugrath JW,Camper DL,Foster ML,Pernich DJ,Walsh TA

更新日期：2004-08-17 00:00:00

abstract：:We have investigated the interaction of alpha 2-macroglobulin (alpha 2M) with the serine proteinase urokinase, an activator of plasminogen. Urokinase formed sodium dodecyl sulfate stable complexes with purified alpha 2M and with alpha 2M in plasma. These complexes could be visualized after polyacrylamide gel electroph...

journal_title：Biochemistry

authors： Straight DL,Hassett MA,McKee PA

更新日期：1985-07-16 00:00:00

abstract：:The observed regioselectivity of the homolytic cleavage of S-adenosylmethionine (SAM) by the radical SAM enzymes is modeled by free radical displacement reactions at sulfoxide centers. These displacements are also regioselective, in direct consequence of the reaction mechanism. The selectivity in the radical SAM react...

journal_title：Biochemistry

authors： Kampmeier JA

更新日期：2010-12-28 00:00:00

abstract：:Protein S is an anticoagulant protein containing a Gla (enclosing gamma-carboxyglutamic acids) module, a TSR (thrombin sensitive region) module, four EGF (epidermal growth factor)-like modules, and a SHBG (sex hormone binding globulin)-like region. Protein S is a cofactor to activated protein C (APC) in the degradatio...

journal_title：Biochemistry

authors： Persson KE,Stenflo J,Linse S,Stenberg Y,Preston RJ,Lane DA,Rezende SM

更新日期：2006-09-05 00:00:00

abstract：:Intact rat liver mitochondria were treated with compounds derived from the reaction of fluorescamine with various types of primary amines, including the mycosamine-containing antibiotics amphotericin B and nystatin. The effect of varying amounts of these compounds on ATPase-linked inorganic phosphate (Pi) formation on...

journal_title：Biochemistry

authors： Ramirez F,Shiuan D,Tu SI,Marecek JF

更新日期：1980-04-29 00:00:00

abstract：:A single amino acid substitution from methionine-184 to valine (M184V) of HIV-1 reverse transcriptase (RT) evokes the 1000-fold 3TC (Lamivudine) resistance by the HIV-1 virus observed in the clinic. The M184V mutant HIV-1 RT was studied to assess its catalytic efficiency during single nucleotide incorporation using a ...

journal_title：Biochemistry

authors： Feng JY,Anderson KS

更新日期：1999-07-20 00:00:00

abstract：:High-resolution proton nuclear magnetic resonance (1H NMR) has been used to study the conformation and dynamics of biosynthetic analogues of actinomycin D that have been substituted at one or both of the 3'-amino acids with azetidine-2-carboxylic acid, pipecolic acid, or 4-ketoproline for one or both of the prolines. ...

journal_title：Biochemistry

authors： Mirau PA,Shafer RH

更新日期：1982-05-25 00:00:00

abstract：:We have determined the mechanism of DNA-dependent protein kinase (DNA-PK) inhibition by cis-diamminedichloroplatinum(II)-(cisplatin-) damaged DNA. We previously have demonstrated that Ku, the DNA binding subunit of DNA-PK, is capable of binding to DNA duplexes globally damaged with cisplatin but was unable to stimulat...

journal_title：Biochemistry

authors： Turchi JJ,Patrick SM,Henkels KM

更新日期：1997-06-17 00:00:00

abstract：:The structure of the subgel phase of dipalmitoylphosphatidylglycerol (DPPG) has been analyzed by X-ray diffraction techniques. Diffraction recorded from highly oriented DPPG specimens in the subgel phase extends to 2-A resolution. There are sharp lamellar reflections on the meridian, and other reflections lie on a ser...

journal_title：Biochemistry

authors： Blaurock AE,McIntosh TJ

更新日期：1986-01-28 00:00:00

abstract：:The direct interrogation of fleeting intermediates by rapid-mixing kinetic methods has significantly advanced our understanding of enzymes that utilize dioxygen. The gas's modest aqueous solubility (<2 mM at 1 atm) presents a technical challenge to this approach, because it limits the rate of formation and extent of a...

journal_title：Biochemistry

authors： Dassama LM,Yosca TH,Conner DA,Lee MH,Blanc B,Streit BR,Green MT,DuBois JL,Krebs C,Bollinger JM Jr

更新日期：2012-02-28 00:00:00

abstract：:Oligodeoxynucleotides (ODNs) were conjugated to chlorambucil and used as affinity labeling reagents to study joint molecule formation by the Escherichia coli recombinase recA. Chlorambucil is a bifunctional nitrogen mustard which alkylates the N-7 position of guanine in the major groove of double-stranded DNA (dsDNA)....

journal_title：Biochemistry

authors： Podyminogin MA,Meyer RB,Gamper HB

更新日期：1995-10-10 00:00:00

abstract：:Casein kinase II is a widely distributed protein serine/threonine kinase. The holoenzyme appears to be a tetramer, containing two alpha or alpha' subunits (or one of each) and two beta subunits. Complementary DNA clones encoding the subunits of casein kinase II were isolated from a human T-cell lambda gt10 library usi...

journal_title：Biochemistry

authors： Lozeman FJ,Litchfield DW,Piening C,Takio K,Walsh KA,Krebs EG

更新日期：1990-09-11 00:00:00

abstract：:A previously unknown polypeptide myotoxin, designated myotoxin a, was isolated for the first time from prairie rattlesnake (Crotalus viridis viridis) venom. Electrophoretic homogeneity of myotoxin a was shown in beta-alanine disc gel polyacrylamide gel electrophoresis and in isoelectric focusing gel electrophoresis. M...

journal_title：Biochemistry

authors： Cameron DL,Tu AT

更新日期：1977-05-31 00:00:00

abstract：:Two glutathione S-transferase (GST) activities have been identified and purified from etiolated corn tissue. The first, designated GST I enzyme, is constitutively present in corn tissue, and the second, designated GST II enzyme, is present only in tissue which has been treated with chemical antidotes which protect cor...

journal_title：Biochemistry

authors： Mozer TJ,Tiemeier DC,Jaworski EG

更新日期：1983-03-01 00:00:00

abstract：:Binding of 125I-labeled human alpha-thrombin to endothelial cells derived from bovine corneas was studied in tissue culture. Specific and saturable binding to the cell surface occurred at 37 degrees C but to a much smaller extent at 4 degrees C. Binding of [125I]thrombin to a specific site on these cells with formatio...

journal_title：Biochemistry

authors： Isaacs JD,Savion N,Gospodarowicz D,Fenton JW 2nd,Shuman MA

更新日期：1981-01-20 00:00:00

abstract：:Double-stranded complementary deoxyribonucleic acid (cDNA) transcribed in vitro from a partially pure myosin light-chain messenger ribonucleic acid (mRNA) of the chick embryonic heart was cloned in Escherichia coli strain chi 1776 by using the HindIII cleavage site in the plasmid pBR322. The insertion of essentially f...

journal_title：Biochemistry

authors： Arnold HH,Siddiqui MA

更新日期：1979-12-11 00:00:00

abstract：:We have mapped the interface between the two transmembrane alpha-helices in the membrane domain of the Escherichia coli enzyme leader peptidase by analyzing disulfides formed between pairs of cysteine residues introduced near their respective periplasmic ends. The interface is formed primarily from aliphatic amino aci...

journal_title：Biochemistry

authors： Whitley P,Nilsson L,von Heijne G

更新日期：1993-08-24 00:00:00

abstract：:O6-Alkylguanine-DNA alkyltransferase (AGT) is an important cellular defense against the mutagenic effects of DNA alkylating agents. In humans this defense can contribute to the ability of some tumors to resist the effects of chemotherapeutic agents that act through DNA alkylation. We report here studies that character...

journal_title：Biochemistry

authors： Fried MG,Kanugula S,Bromberg JL,Pegg AE

更新日期：1996-12-03 00:00:00

abstract：:By limited proteolysis of a type kappa immunoglobulin light chain (Oku) with clostripain, both the VL and CL fragments were obtained with a high yield. The unfolding and refolding by guanidine hydrochloride of light chain Oku and its VL and CL fragments were studied at pH 7.5 and 25 degrees C with circular dichroism a...

journal_title：Biochemistry

authors： Tsunenaga M,Goto Y,Kawata Y,Hamaguchi K

更新日期：1987-09-22 00:00:00

abstract：:Replication protein A (RPA) is a heterotrimeric protein that is required for DNA replication and most DNA repair pathways. RPA has previously been shown to play a role in recognizing and binding damaged DNA during nucleotide excision repair (NER). RPA has also been suggested to play a role in psoralen DNA interstrand ...

journal_title：Biochemistry

authors： Patrick SM,Tillison K,Horn JM

更新日期：2008-09-23 00:00:00

abstract：:The neutron structure of subtilisin BPN' has been refined and analyzed at 2.0-A resolution. The structure studied was a mutant variant of subtilisin, Met222----Gln, and was used because large, uninhibited crystals could be grown, which was not the case for the native molecule. Comparison of the structure with that of ...

journal_title：Biochemistry

authors： Kossiakoff AA,Ultsch M,White S,Eigenbrot C

更新日期：1991-02-05 00:00:00

abstract：:The carbon-13 (13C) nuclear magnetic resonance (NMR) behavior of the hyperactive analogue of luteinizing-hormone-releasing hormone (LH-RH), des-Gly-NH2(10)-[D-Leu6]LH-RH ethylamide, has been studied. The spectra are compared with those of the hypoactive des-Gly-NH2(10)-[L-Leu6]LH-RH ethylamide. Spin-lattice relaxation...

journal_title：Biochemistry

authors： Deslauriers R,Komoroski RA,Levy GC,Seely JH,Smith IC

更新日期：1976-10-19 00:00:00

abstract：:Isoaspartyl dipeptidase (IAD) is a member of the amidohydrolase superfamily and catalyzes the hydrolytic cleavage of beta-aspartyl dipeptides. Structural studies of the wild-type enzyme have demonstrated that the active site consists of a binuclear metal center positioned at the C-terminal end of a (beta/alpha)(8)-bar...

journal_title：Biochemistry

authors： Martí-Arbona R,Fresquet V,Thoden JB,Davis ML,Holden HM,Raushel FM

更新日期：2005-05-17 00:00:00

abstract：:The rates of inactivation of BF1 adenosinetriphosphatase (BF1-ATPase) from Escherichia coli by 7-chloro-4-nitro-2,1,3-benzoxadiazole, 1-fluoro-2,4-dinitrobenzene, 2,4,6-trinitrobenzenesulfonate, phenylglyoxal, and N,N'-dicyclohexylcarbodiimide have been measured in the presence and absence of various concentrations of...

journal_title：Biochemistry

authors： Ting LP,Wang JH

更新日期：1982-01-19 00:00:00

abstract：:Biotin synthase, a member of the "radical SAM" family, catalyzes the final step of the biotin biosynthetic pathway, namely, the insertion of a sulfur atom into dethiobiotin (DTB). The active form of the enzyme contains two iron-sulfur clusters, a [4Fe-4S](2+) cluster liganded by Cys-53, Cys-57, and Cys-60 and the S-ad...

journal_title：Biochemistry

authors： Lotierzo M,Raux E,Tse Sum Bui B,Goasdoue N,Libot F,Florentin D,Warren MJ,Marquet A

更新日期：2006-10-10 00:00:00

abstract：:The folding of the enzyme indole-3-glycerol-phosphate synthase (IGPS), a member of the (alpha/beta)8 fold family, has been studied. At least two folding intermediates have been detected using spectroscopic and activity measurements in combination with gel filtration chromatography. These two intermediates are produced...

journal_title：Biochemistry

authors： Sánchez del Pino MM,Fersht AR

更新日期：1997-05-06 00:00:00

abstract：:The nuclei and chromatin of rat liver contain three major proteins reacting with diisopropyl fluorophosphate (DFP). The molecular weights of the three proteins determined by gel filtration in the presence of sodium dodecyl sulfate and sodium dodecyl sulfate-polyacrylamide gel electrophoresis are 70000, 60000, and 250...

journal_title：Biochemistry

authors： Carter DB,Efird PH,Chae CB

更新日期：1976-06-15 00:00:00