当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > O(2)-evolving chlorite dismutase as a tool for studying O(2)-utilizing enzymes.

O(2)-evolving chlorite dismutase as a tool for studying O(2)-utilizing enzymes.

Abstract：

:The direct interrogation of fleeting intermediates by rapid-mixing kinetic methods has significantly advanced our understanding of enzymes that utilize dioxygen. The gas's modest aqueous solubility (<2 mM at 1 atm) presents a technical challenge to this approach, because it limits the rate of formation and extent of accumulation of intermediates. This challenge can be overcome by use of the heme enzyme chlorite dismutase (Cld) for the rapid, in situ generation of O(2) at concentrations far exceeding 2 mM. This method was used to define the O(2) concentration dependence of the reaction of the class Ic ribonucleotide reductase (RNR) from Chlamydia trachomatis, in which the enzyme's Mn(IV)/Fe(III) cofactor forms from a Mn(II)/Fe(II) complex and O(2) via a Mn(IV)/Fe(IV) intermediate, at effective O(2) concentrations as high as ~10 mM. With a more soluble receptor, myoglobin, an O(2) adduct accumulated to a concentration of >6 mM in <15 ms. Finally, the C-H-bond-cleaving Fe(IV)-oxo complex, J, in taurine:α-ketoglutarate dioxygenase and superoxo-Fe(2)(III/III) complex, G, in myo-inositol oxygenase, and the tyrosyl-radical-generating Fe(2)(III/IV) intermediate, X, in Escherichia coli RNR, were all accumulated to yields more than twice those previously attained. This means of in situ O(2) evolution permits a >5 mM "pulse" of O(2) to be generated in <1 ms at the easily accessible Cld concentration of 50 μM. It should therefore significantly extend the range of kinetic and spectroscopic experiments that can routinely be undertaken in the study of these enzymes and could also facilitate resolution of mechanistic pathways in cases of either sluggish or thermodynamically unfavorable O(2) addition steps.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Dassama LM,Yosca TH,Conner DA,Lee MH,Blanc B,Streit BR,Green MT,DuBois JL,Krebs C,Bollinger JM Jr

doi

10.1021/bi201906x

subject

Has Abstract

pub_date

2012-02-28 00:00:00

pages

1607-16

issue

8

eissn

0006-2960

issn

1520-4995

journal_volume

51

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • The amino acid sequence of chick skin collagen alpha1-CB7.

    abstract：:The amino acid sequence of chick skin collagen alpha1-CB7, the 268 CNBr peptide from the helical portion of the alpha1 chain, has been determined by automatic and manual degradation of tryptic and chymotryptic peptides, and of the COOH-terminal fragment produced by cleavage with animal collagenase. The resulting seque...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00684a013

    authors： Highberger JH,Corbett C,Kang AH,Gross J

    更新日期：1975-07-01 00:00:00

  • Structural analysis of ADP-glucose pyrophosphorylase from the bacterium Agrobacterium tumefaciens.

    abstract：:ADP-glucose pyrophosphorylase (ADPGlc PPase) catalyzes the conversion of glucose 1-phosphate and ATP to ADP-glucose and pyrophosphate. As a key step in glucan synthesis, the ADPGlc PPases are highly regulated by allosteric activators and inhibitors in accord with the carbon metabolism pathways of the organism. Crystal...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi701933q

    authors： Cupp-Vickery JR,Igarashi RY,Perez M,Poland M,Meyer CR

    更新日期：2008-04-15 00:00:00

  • Nucleotide binding to the multidrug resistance P-glycoprotein as studied by ESR spectroscopy.

    abstract：:Electron spin resonance (ESR) spectroscopy using spin-labeled ATP was used to study nucleotide binding to and structural transitions within the multidrug resistance P-glycoprotein, P-gp. Spin-labeled ATP (SL-ATP) with the spin label attached to the ribose, was observed to be an excellent substrate analogue for P-gp. S...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0512445

    authors： Delannoy S,Urbatsch IL,Tombline G,Senior AE,Vogel PD

    更新日期：2005-10-25 00:00:00

  • Structure of Toxoplasma gondii LDH1: active-site differences from human lactate dehydrogenases and the structural basis for efficient APAD+ use.

    abstract：:While within a human host the opportunistic pathogen Toxoplasma gondii relies heavily on glycolysis for its energy needs. Lactate dehydrogenase (LDH), the terminal enzyme in anaerobic glycolysis necessary for NAD(+) regeneration, therefore represents an attractive therapeutic target. The tachyzoite stage lactate dehyd...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi035108g

    authors： Kavanagh KL,Elling RA,Wilson DK

    更新日期：2004-02-03 00:00:00

  • Identification of two tetranuclear FeS clusters on the ferredoxin-type subunit of NADH:ubiquinone oxidoreductase (complex I).

    abstract：:The proton-translocating NADH:ubiquinone oxidoreductase of respiratory chains (complex I) contains one flavin mononucleotide and five EPR-detectable iron-sulfur clusters as redox groups. Because of the number of conserved motifs typical for binding iron-sulfur clusters and the high content of iron and acid-labile sulf...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0026977

    authors： Rasmussen T,Scheide D,Brors B,Kintscher L,Weiss H,Friedrich T

    更新日期：2001-05-22 00:00:00

  • Binding of ATP by pertussis toxin and isolated toxin subunits.

    abstract：:The binding of ATP to pertussis toxin and its components, the A subunit and B oligomer, was investigated. Whereas, radiolabeled ATP bound to the B oligomer and pertussis toxin, no binding to the A subunit was observed. The binding of [3H]ATP to pertussis toxin and the B oligomer was inhibited by nucleotides. The relat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00478a003

    authors： Hausman SZ,Manclark CR,Burns DL

    更新日期：1990-07-03 00:00:00

  • Double-strand break formation during nucleotide excision repair of a DNA interstrand cross-link.

    abstract：:The DNA interstrand cross-link (ICL) resulting from the C4'-oxidized abasic site (C4-AP) is a unique clustered lesion comprised of a cross-link adjacent to a nick. The ICL is a substrate for the UvrABC nucleotide excision repair system. The strand containing the nick is preferentially incised, but the nick influences ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi901006b

    authors： Sczepanski JT,Jacobs AC,Van Houten B,Greenberg MM

    更新日期：2009-08-18 00:00:00

  • Intramolecular proton transfer from multiple sites in catalysis by murine carbonic anhydrase V.

    abstract：:The hydration of CO2 catalyzed by carbonic anhydrase requires proton transfer from the zinc-bound water at the active site to solution for each cycle of catalysis. In the most efficient of the mammalian carbonic anhydrases, isozyme II, this transfer is facilitated by a proton shuttle residue, His 64. Murine carbonic a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9729209

    authors： Earnhardt JN,Qian M,Tu C,Laipis PJ,Silverman DN

    更新日期：1998-05-19 00:00:00

  • The membrane-bound quinohemoprotein alcohol dehydrogenase from Gluconacetobacter diazotrophicus PAL5 carries a [2Fe-2S] cluster.

    abstract：:Gluconacetobacter diazotrophicus stands out among the acetic acid bacteria as it fixes dinitrogen and is a true endophyte. It has a set of constitutive enzymes to oxidize ethanol and acetaldehyde which is upregulated during N(2)-dependent growth. The membrane-bound alcohol dehydrogenase (ADH) is a heterodimer (subunit...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9015007

    authors： Gómez-Manzo S,Solano-Peralta A,Saucedo-Vázquez JP,Escamilla-Marván JE,Kroneck PM,Sosa-Torres ME

    更新日期：2010-03-23 00:00:00

  • Stabilization of the red semiquinone form of pig kidney general acyl-CoA dehydrogenase by acyl coenzyme A derivatives.

    abstract：:Pig kidney general acyl-CoA dehydrogenases forms the blue neutral radical on dithionite or photochemical reduction (Thorpe, C., Matthews, R. G., & Williams, C. H. (1979) Biochemistry 18, 331-337] in accord with its classification as a flavoprotein dehydrogenase. However, dithionite reduction of the enzyme in the prese...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00520a024

    authors： Mizzer JP,Thorpe C

    更新日期：1981-08-18 00:00:00

  • Casein kinase II phosphorylation of caldesmon downregulates myosin-caldesmon interactions.

    abstract：:It is well-known that caldesmon (CaD) is a substrate for casein kinase II (CKII), and the phosphorylation of CaD by CKII regulates the interaction of CaD with myosin. However, the functionally relevant CKII phosphorylation site(s) on CaD and the precise role of CaD phosphorylation by CKII in mediating CaD's function h...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0006767

    authors： Wang Z,Yang ZQ

    更新日期：2000-09-12 00:00:00

  • Characteristics of the isolated apical plasmalemma and intracellular tubulovesicles of the gastric acid secreting cells: demonstration of secretagogue-induced membrane mobilization.

    abstract：:Separation of the gradient-purified gastric microsome into two membrane subfractions of distinct enzymatic and phospholipid composition has been achieved by mild SDS (0.033% w/v) treatment followed by sucrose gradient centrifugation of the pig and rabbit gastric microsomes. While the high-density membranes had all of ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00425a013

    authors： Ray TK,Das PK,Nandi J,Banerjee A,Bandopadhyay S

    更新日期：1988-12-13 00:00:00

  • Interactions between phospholipids and NADH:ubiquinone oxidoreductase (complex I) from bovine mitochondria.

    abstract：:NADH:ubiquinone oxidoreductase (complex I) from bovine heart mitochondria is a highly complicated, energy transducing, membrane-bound enzyme. It contains 46 different subunits and nine redox cofactors: a noncovalently bound flavin mononucleotide and eight iron-sulfur clusters. The mechanism of complex I is not known. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi051809x

    authors： Sharpley MS,Shannon RJ,Draghi F,Hirst J

    更新日期：2006-01-10 00:00:00

  • Insulin signaling in the yeast Saccharomyces cerevisiae. 1. Stimulation of glucose metabolism and Snf1 kinase by human insulin.

    abstract：:Effects of human insulin on glucose metabolism in the yeast Saccharomyces cerevisiae were studied in this report. Under two conditions of growth limitation (glucose-grown cells during transition to stationary phase or spheroplasts during incubation in synthetic glucose medium), human insulin (10 and 1 microM, respecti...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi972071p

    authors： Müller G,Rouveyre N,Crecelius A,Bandlow W

    更新日期：1998-06-16 00:00:00

  • Hidden Conformational States and Strange Temperature Optima in Enzyme Catalysis.

    abstract：:The existence of temperature optima in enzyme catalysis that occur before protein melting sets in can be described by different types of kinetic models. Such optima cause distinctly curved Arrhenius plots and have, for example, been observed in several cold-adapted enzymes from psychrophilic species. The two main expl...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.0c00705

    authors： Åqvist J,Sočan J,Purg M

    更新日期：2020-10-13 00:00:00

  • Redox regulation of epidermal growth factor receptor signaling through cysteine oxidation.

    abstract：:Epidermal growth factor receptor (EGFR) exemplifies the family of receptor tyrosine kinases that mediate numerous cellular processes, including growth, proliferation, and differentiation. Moreover, gene amplification and EGFR mutations have been identified in a number of human malignancies, making this receptor an imp...

    journal_title：Biochemistry

    pub_type： 杂志文章,评审

    doi：10.1021/bi301441e

    authors： Truong TH,Carroll KS

    更新日期：2012-12-18 00:00:00

  • Equilibrium folding studies of tetrameric R67 dihydrofolate reductase.

    abstract：:R67 dihydrofolate reductase (DHFR) is an R-plasmid encoded enzyme that confers resistance to the antibacterial drug trimethoprim. This enzyme is not homologous in sequence or structure to chromosomal DHFRs. Equilibrium folding of tetrameric R67 DHFR was studied and found to be fully reversible. Formation of an inactiv...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00180a018

    authors： Zhuang P,Eisenstein E,Howell EE

    更新日期：1994-04-12 00:00:00

  • Identification and synthesis of a naturally occurring selenonucleoside in bacterial tRNAs: 5-[(methylamino)methyl]-2-selenouridine.

    abstract：:Escherichia coli, Clostridium sticklandii, and Methanococcus vannielii synthesize 75Se-labeled amino acid transfer ribonucleic acids [( 75Se]tRNAs) when grown with low levels (approximately equal to 1 microM) of 75SeO32-. When E. coli [75Se]tRNA was digested to nucleosides and analyzed by reversed-phase high-performan...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00315a021

    authors： Wittwer AJ,Tsai L,Ching WM,Stadtman TC

    更新日期：1984-09-25 00:00:00

  • Ethanol enhances agonist-induced fast desensitization in nicotinic acetylcholine receptors.

    abstract：:The reversible decline of the nicotinic acetylcholine receptor's response to acetylcholine during prolonged exposure to acetylcholine is known as desensitization. Here, we studied ethanol's modulation of fast agonist-induced desensitization of the nicotinic acetylcholine receptor in postsynaptic membrane vesicles from...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00197a009

    authors： Wu G,Miller KW

    更新日期：1994-08-09 00:00:00

  • In vitro kinetic analysis of substrate specificity in enterobactin biosynthetic lower pathway enzymes provides insight into the biochemical function of the hot dog-fold thioesterase EntH.

    abstract：:The Escherichia coli siderophore enterobactin is assembled from 2,3-dihydroxybenzoate (2,3-DHB) and l-serine by the nonribosomal peptide synthetases EntB and EntF. The processive thiol-template strategy used can be sabotaged by EntB misacylation. Through in vitro kinetic analysis, we demonstrate two potential routes t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi802207t

    authors： Chen D,Wu R,Bryan TL,Dunaway-Mariano D

    更新日期：2009-01-27 00:00:00

  • A Haemophilus influenzae strain associated with Fisher syndrome expresses a novel disialylated ganglioside mimic.

    abstract：:The non-typeable Haemophilus influenzae strain DH1 was isolated from a 25 year old male patient with Fisher syndrome, a postinfectious autoimmune condition characterized by the presence of anti-GQ1b IgG antibodies that target and initiate damage to peripheral nerves. DH1 was found to display an alphaNeuAc(2-8)alphaNeu...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi700685s

    authors： Houliston RS,Koga M,Li J,Jarrell HC,Richards JC,Vitiazeva V,Schweda EK,Yuki N,Gilbert M

    更新日期：2007-07-10 00:00:00

  • Opossum serum alpha 1-proteinase inhibitor: purification, linear sequence, and resistance to inactivation by rattlesnake venom metalloproteinases.

    abstract：:Opossum (Didelphis virginiana) serum was fractionated with (NH4)2SO4 and then chromatographed on DEAE-Sepharose and phenyl-Sepharose. Affinity chromatography on a protein A-Sepharose-antibody column removed traces of opossum serum metalloproteinase inhibitors, and resulted in a homogeneous preparation of opossum alpha...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00053a015

    authors： Catanese JJ,Kress LF

    更新日期：1993-01-19 00:00:00

  • Identification of effector binding sites on S100 beta: studies with guanylate cyclase and p80, a retinal phosphoprotein.

    abstract：:S100 beta is a calcium-binding protein, which regulates the activities of several enzymes and inhibits the phosphorylation of a variety of protein kinase C substrates in a calcium-dependent manner. The protein was recently found to activate a retinal membrane guanylate cyclase, and in this paper, we report that it inh...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9802115

    authors： Pozdnyakov N,Margulis A,Sitaramayya A

    更新日期：1998-07-28 00:00:00

  • Local and global dynamics of the G protein-coupled receptor CXCR1.

    abstract：:The local and global dynamics of the chemokine receptor CXCR1 are characterized using a combination of solution NMR and solid-state NMR experiments. In isotropic bicelles (q = 0.1), only 13% of the expected number of backbone amide resonances is observed in (1)H/(15)N HSQC solution NMR spectra of uniformly (15)N-label...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi101568j

    authors： Park SH,Casagrande F,Das BB,Albrecht L,Chu M,Opella SJ

    更新日期：2011-03-29 00:00:00

  • Identification of active site residues essential to 4-chlorobenzoyl-coenzyme A dehalogenase catalysis by chemical modification and site directed mutagenesis.

    abstract：:4-Chlorobenzoyl-coenzyme A (4-CBA-CoA) dehalogenase catalyzes the hydrolysis of 4-CBA-CoA to 4-hydroxybenzoyl-coenzyme A (4-HBA-CoA) via a nucleophilic aromatic substitution pathway involving the participation of an active site carboxylate side chain in covalent catalysis. In this paper we report on the identification...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9609533

    authors： Yang G,Liu RQ,Taylor KL,Xiang H,Price J,Dunaway-Mariano D

    更新日期：1996-08-20 00:00:00

  • Crystal structure of human fibrinogen.

    abstract：:A crystal structure of human fibrinogen has been determined at approximately 3.3 A resolution. The protein was purified from human blood plasma, first by a cold ethanol precipitation procedure and then by stepwise chromatography on DEAE-cellulose. A product was obtained that was homogeneous on SDS-polyacrylamide gels....

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi802205g

    authors： Kollman JM,Pandi L,Sawaya MR,Riley M,Doolittle RF

    更新日期：2009-05-12 00:00:00

  • Entropy effects on protein hinges: the reaction catalyzed by triosephosphate isomerase.

    abstract：:Many proteins utilize segmental motions to catalyze a specific reaction. The Omega loop of triosephosphate isomerase (TIM) is important for preventing the loss of the reactive enediol(ate) intermediate. The loop opens and closes even in the absence of the ligand, and the loop itself does not change conformation during...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049208d

    authors： Xiang J,Jung JY,Sampson NS

    更新日期：2004-09-14 00:00:00

  • Escherichia coli glycerol kinase: role of a tetramer interface in regulation by fructose 1,6-bisphosphate and phosphotransferase system regulatory protein IIIglc.

    abstract：:Escherichia coli glycerol kinase (EC 2.7.1.30; ATP:glycerol 3-phosphotransferase) is a key element in a signal transduction pathway that couples expression of genes required for glycerol metabolism to the relative availability of glycerol and glucose. Its catalytic activity is inhibited by protein-protein interactions...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00199a040

    authors： Liu WZ,Faber R,Feese M,Remington SJ,Pettigrew DW

    更新日期：1994-08-23 00:00:00

  • Interconversion of conformational isomers of light chains in the Mcg immunoglobulins.

    abstract：:Previous crystallographic studies in this laboratory demonstrated that immunoglobulin light chains with the same amino acid sequence can have at least two and probably three or more conformations, depending on whether the second member of an interacting pair is a light or heavy chain. If a heavy chain is not available...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00594a022

    authors： Firca JR,Ely KR,Kremser P,Westholm FA,Dorrington KJ,Edmundson AB

    更新日期：1978-01-10 00:00:00

  • Proton and deuteron nuclear magnetic relaxation dispersion studies of Ca2+-Mn2+-lentil lectin and Ca2+-Mn2+-pea lectin: evidence for a site of solvent exchange in common with concanavalin A.

    abstract：:Measurements of the magnetic field dependence of the longitudinal magnetic relaxation rates (NMRD profiles) of solvent protons and deuterons led to the discovery of two classes of solvent binding sites in Ca2+-Mn2+-concanavalin A (CMPL) [Koenig, S. H., Brown, R. D., III, & Brewer, C. F. (1985) Biochemistry (second of ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00340a004

    authors： Bhattacharyya L,Brewer CF,Brown RD 3rd,Koenig SH

    更新日期：1985-09-10 00:00:00