Abstract:
:Measurements of the magnetic field dependence of the longitudinal magnetic relaxation rates (NMRD profiles) of solvent protons and deuterons led to the discovery of two classes of solvent binding sites in Ca2+-Mn2+-concanavalin A (CMPL) [Koenig, S. H., Brown, R. D., III, & Brewer, C. F. (1985) Biochemistry (second of three papers in this issue)]. In this paper, we compare proton and deuteron NMRD profiles of Ca2+-Mn2+-lentil lectin (CMLcH) and Ca2+-Mn2+-pea lectin (CMPSA) with those of CMPL. All three metalloproteins are D-mannose/D-glucose-specific lectins that have a high degree of structural similarity and require the metal ions for their biological activities. We have developed a method for the preparation of fully active metal ion derivatives of lentil lectin (LcH) and pea lectin (PSA), including the diamagnetic derivatives Ca2+-Zn2+-LcH and Ca2+-Zn2+-PSA [Bhattacharyya, L., Brewer, C. F., Brown, R. D., III, & Koenig, S. H.(1984) Biochem. Biophys. Res. Commun. 124, 857-862]. The behavior of these two lectins with regard to their NMRD profiles is essentially identical, for both the paramagnetic and diamagnetic forms. Together with CMPL, all three lectins have a common paramagnetic contribution with a negative temperature dependence of the rates, while CMPL contributes an additional component with a positive temperature dependence. The common contribution derives from the class of fast exchanging water molecules observed in the proton NMRD profile of CMPL (Koenig et al., 1985); their protons are calculated to be relatively remote from the Mn2+ ions (4.4 A for CMPL and 5.5 A for LcH and PSA).(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Bhattacharyya L,Brewer CF,Brown RD 3rd,Koenig SHdoi
10.1021/bi00340a004subject
Has Abstractpub_date
1985-09-10 00:00:00pages
4985-90issue
19eissn
0006-2960issn
1520-4995journal_volume
24pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::A general understanding of how cytochromes evolve within a fixed structure to optimize redox potential for specific bioenergetic processes does not exist. Toward this end, a library approach is used to investigate the range and distribution of redox potential which occurs when all sequence space available through muta...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0001675
更新日期:2000-05-23 00:00:00
abstract::Autophagy, an essential eukaryotic homeostasis pathway, allows the sequestration of unwanted, damaged, or harmful cytoplasmic components in vesicles called autophagosomes, permitting subsequent lysosomal degradation and nutrient recycling. Autophagosome nucleation is mediated by class III phosphatidylinositol-3-kinase...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00246
更新日期:2016-08-02 00:00:00
abstract::The multifunctional proline utilization A (PutA) flavoenzyme from Escherichia coli catalyzes the oxidation of proline to glutamate in two reaction steps using separate proline dehydrogenase (PRODH) and Δ(1)-pyrroline-5-carboxylate (P5C) dehydrogenase domains. Here, the kinetic mechanism of PRODH in PutA is studied by ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201603f
更新日期:2012-01-10 00:00:00
abstract::Biotin synthase, the enzyme which catalyzes the last step of the biosynthesis of biotin, contains only (2Fe-2S)(2+) clusters when isolated under aerobic conditions. Previous results showed that reduction by dithionite or photoreduced deazaflavin converts the (2Fe-2S)(2+) to (4Fe-4S)(2+,+). However, until now, no detai...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034426c
更新日期:2003-07-29 00:00:00
abstract::Heme-regulated eIF2alpha kinase [heme-regulated inhibitor (HRI)] plays a critical role in the regulation of protein synthesis by heme iron. The kinase active site is located in the C-terminal domain, whereas the N-terminal domain is suggested to regulate catalysis in response to heme binding. Here, we found that the r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060556k
更新日期:2006-08-15 00:00:00
abstract::PsbO, the photosystem II manganese stabilizing protein, contains an aspartate residue [Asp157 (spinach numbering)], which is highly conserved in eukaryotic and prokaryotic PsbOs. The homology model of the PSII-bound conformation of spinach PsbO presented here positions Asp157 in the large flexible loop of the protein....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9016999
更新日期:2009-12-22 00:00:00
abstract::Botulinum neurotoxins (BoNTs) elicit flaccid paralysis through cleavage of SNARE proteins within peripheral neurons. There are seven serotypes of the BoNTs, termed A-G, which differ in the SNARE protein and/or site that is cleaved. BoNTs are single-chain toxins that comprise an N-terminal zinc metalloprotease domain t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi602396x
更新日期:2007-03-20 00:00:00
abstract::The cardiac-specific Nkx2.5 homeodomain has been expressed as a 79-residue protein with the oxidizable Cys(56) replaced with Ser. The Nkx2.5 or Nkx2.5(C56S) homeodomain is 73% identical in sequence to and has the same NMR structure as the vnd (ventral nervous system defective)/NK-2 homeodomain of Drosophila when bound...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi050835s
更新日期:2005-09-20 00:00:00
abstract::A topoisomer gel retardation assay has been used to examine the topological requirements for the formation of open promoter complexes on DNA minicircles carrying sigma 54-dependent promoters. In the absence of intercalators, individual topoisomers carrying both the nifL and nifF promoters could be resolved as discrete...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9701179
更新日期:1997-10-07 00:00:00
abstract::The interactions of bovine brain MAP2 and tau, recombinant murine MAP2C, and recombinant human tau with phosphatidylinositol vesicles yield apparent Kd values of 51 +/- 6 nM, 2.4 +/- 0.6 microM, 1.4 +/- 0.1 microM, and 1.6 +/- 0.2 microM, respectively. Examinations of the binding of MAP2 and/or MAP2C to phosphatidylch...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00192a009
更新日期:1994-07-05 00:00:00
abstract::The extrinsic 33-kDa protein (P33) was cross-linked covalently to the binding site on P33-depleted PSII preparations which is responsible for reconstitution of photosynthetic water oxidation after PSII preparations have been washed with 1 M CaCl2. Conditions were found in which more than half of the cross-linked prote...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00439a033
更新日期:1989-06-27 00:00:00
abstract::A generalized set of fluorescence polarization intensities and electron paramagnetic resonance (EPR) spectra from multiple fluorescent and EPR probes of a specific site on an oriented and immobilized protein element in a biological assembly, combined with anisotropic rotational relaxation studies of the purified and l...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00184a004
更新日期:1994-05-10 00:00:00
abstract::Site-directed mutagenesis has been used to construct three recombinant mutant hemoglobins (rHbs), rHb(beta L105W), rHb(alpha D94A/betaL105W), and rHb(alpha D94A). rHb(beta L105W) is designed to form a new hydrogen bond from beta 105Trp to alpha 94Asp in the alpha(1)beta(2) subunit interface to lower the oxygen binding...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001115i
更新日期:2000-11-14 00:00:00
abstract::Well-ordered multilamellar arrays of liquid-crystalline phosphatidylcholine and equimolar phosphatidylcholine-cholesterol bilayers have been formed in the nonaqueous solvents formamide and 1,3-propanediol. The organization of these bilayers and the interactions between apposing bilayer surfaces have been investigated ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00445a053
更新日期:1989-09-19 00:00:00
abstract::Fluorescence studies of the binding of IKe gene 5 protein to various polynucleotides were performed to obtain insight into the question as to what extent the binding characteristics of the gene 5 proteins of the IKe and M13 phages resemble and/or differ from each other. The fluorescence of IKe gene 5 protein is quench...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00381a037
更新日期:1987-04-07 00:00:00
abstract::BtuB is a TonB-dependent transport protein that binds and carries vitamin B(12) across the outer membrane of Gram negative bacteria such as Escherichia coli. Previous work has demonstrated that the Ton box, a highly conserved segment near the N-terminus of the protein, undergoes an order-to-disorder transition upon th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061051x
更新日期:2006-09-12 00:00:00
abstract::This paper characterizes the low-affinity ligand binding interactions of a fluorinated volatile anesthetic, isoflurane (CHF2OCHClCF3), with bovine serum albumin (BSA) using 19F-NMR transverse relaxation (T2). 19F-NMR spectra of isoflurane in aqueous BSA reveal a single isoflurane trifluoromethyl resonance, indicative ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00146a007
更新日期:1992-08-11 00:00:00
abstract::F-STOP is a microtubule-associated protein that stabilizes microtubules in a calmodulin (CaM)-dependent manner. All members of the stable tubule only polypeptide (STOP) family have a central domain that contains nearly identical multiple repeats, and a CaM binding motif is present in multiple copies within this domain...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034746w
更新日期:2003-10-07 00:00:00
abstract::In aspartate aminotransferase, pyridoxal 5'-phosphate (PLP) forms a Schiff base with the epsilon-amino group of Lys258 (internal aldimine). The internal aldimine has a pKa value of 6.8. Binding of a substrate amino acid to the enzyme yields the Michaelis complex, in which PLP still forms the internal aldimine with Lys...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981517e
更新日期:1998-10-27 00:00:00
abstract::Minor groove specific DNA equilibrium binding peptides (lex) based on N-methylpyrrole-carboxamide and/or N-methylimidazolecarboxamide subunits have been modified with an O-methyl sulfonate ester functionality to target DNA methylation in the minor groove at Ade/Thy- and/or Gua/Cyt-rich sequences. HPLC and sequencing g...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0353272
更新日期:2003-12-09 00:00:00
abstract::Two successive steps in de novo purine biosynthesis are catalyzed by the enzymes 5-aminoimidazole ribonucleotide (AIR) carboxylase and 4-[(N-succinylamino)carbonyl]-5-aminoimidazole ribonucleotide (SAICAR) synthetase. Amino acid sequence alignments of the proteins from various sources suggested that several unusual di...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00205a030
更新日期:1994-10-04 00:00:00
abstract::Cholesterol (Chol) in phosphatidylcholine large unilamellar vesicles (PC LUV) modulated interaction of the bilayers with a class A amphipathic peptide, Ac-18A-NH2: Chol increased the peptide binding capacity and reduced the affinity together with the peptide-induced leakage of calcein from LUV. Similar effects of Chol...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011885+
更新日期:2002-03-26 00:00:00
abstract::A methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase (MeTr) from Clostridium thermoaceticum catalyzes the transfer of the N5 methyl group from (6S)-methyltetrahydrofolate (CH3-H4folate) to the cobalt center of a corrinoid/iron-sulfur protein (C/Fe-SP). The methylcobamide product is the first in a s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00046a013
更新日期:1995-11-21 00:00:00
abstract::Proteins denature at low pH because of intramolecular electrostatic repulsions. The addition of salt partially overcomes this repulsion for some proteins, yielding a collapsed conformation called the A-state. A-states have characteristics expected for the molten globule, a notional kinetic protein folding intermediate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981364v
更新日期:1998-12-01 00:00:00
abstract::The binding of UDP-N-acetylglucosamine (UDPNAG) to the enzyme UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) was studied in the absence and presence of the antibiotic fosfomycin by isothermal titration calorimetry. Fosfomycin binds covalently to MurA in the presence of UDPNAG and also in its absence as demonst...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0107041
更新日期:2001-08-21 00:00:00
abstract::Steroid-receptor complexes formed at low temperature and ionic strength are unable to bind to target cell nuclei. After a temporary exposure to high ionic strength and/or temperature they become activated (i.e., able to bind to nuclei). However, there exists an equilibrium between activated and nonactivated complexes;...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00664a025
更新日期:1976-09-21 00:00:00
abstract::Adipocyte lipid-binding protein (ALBP) is the adipocyte member of an intracellular hydrophobic ligand-binding protein family. ALBP is phosphorylated by the insulin receptor kinase upon insulin stimulation. The crystal structure of recombinant murine ALBP has been determined and refined to 2.5 A. The final R factor for...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00128a024
更新日期:1992-04-07 00:00:00
abstract::The Rieske protein from Thermus thermophilus (TtRp) and a truncated version of the protein (truncTtRp), produced to achieve a low-pH crystallization condition, have been characterized using UV-visible and circular dichroism spectroscopies. TtRp and truncTtRp undergo a change in the UV-visible spectra with increasing p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901126u
更新日期:2009-10-20 00:00:00
abstract::We have examined the C cluster in type II CO dehydrogenase (CODH) from Carboxydothermus hydrogenformans using Ni K-edge X-ray absorption near edge spectroscopy and extended X-ray absorption fine structure (EXAFS) spectroscopy. The enzyme was studied under three conditions: "as-isolated" and after treatment with CO or ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi036104e
更新日期:2004-07-20 00:00:00
abstract::We have used limited proteolysis of affinity-labeled estrogen receptors (ER), coupled with antireceptor antibody immunoreactivity, to assess structural features of ER and the relatedness of ER from MCF-7 human breast cancer and rat uterine cells. MCF-7 ER preparations covalently labeled with [3H]tamoxifen aziridine [(...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00382a043
更新日期:1987-04-21 00:00:00