Following the rotational trajectory of the principal hydrodynamic frame of a protein using multiple probes.

abstract：:All eight cysteine residues, C90, C94, C143, C147, C219, C325, C367, and C431, present in transmembrane domains of the Aspergillus nidulans NrtA nitrate transporter protein were altered individually by site-specific mutagenesis. The results indicate that six residues, C90, C147, C219, C325, C367, and C431, are not req...

journal_title：Biochemistry

authors： Unkles SE,Rouch DA,Wang Y,Siddiqi MY,Okamoto M,Stephenson RM,Kinghorn JR,Glass AD

更新日期：2005-04-12 00:00:00

abstract：:Pokeweed antiviral protein (PAP) is a single-chain ribosome inactivating protein (RIP) that binds to ribosomes and depurinates the highly conserved alpha-sarcin/ricin loop (SRL) of the large subunit rRNA. Catalytic depurination of a specific adenine has been proposed to result in translation arrest and cytotoxicity. H...

journal_title：Biochemistry

authors： Parikh BA,Baykal U,Di R,Tumer NE

更新日期：2005-02-22 00:00:00

abstract：:The two distinct domains of flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase, EC 1.1.2.3) are connected by a typical hinge peptide. To probe the importance of the structural integrity of the hinge region for efficient intraprotein electron transfer, three mutant enzymes have been constructed: H delta 3 [Sharp...

journal_title：Biochemistry

authors： Sharp RE,Chapman SK,Reid GA

更新日期：1996-01-23 00:00:00

abstract：:In type 2 diabetics, the hormone amylin misfolds into amyloid plaques implicated in the destruction of the pancreatic β-cells that make insulin and amylin. The aggregative misfolding of amylin is pH-dependent, and exposure of the hormone to acidic and basic environments could be physiologically important. Amylin has t...

journal_title：Biochemistry

authors： Jha S,Snell JM,Sheftic SR,Patil SM,Daniels SB,Kolling FW,Alexandrescu AT

更新日期：2014-01-21 00:00:00

abstract：:The luminescence of Cu-substituted cytochrome c exhibits temperature-dependent decay modes and spectral shifts at temperatures below 80 K. By comparison with the data from metal-substituted porphyrins, we determined intramolecular rate parameters. The observed tripdoublet decay time is 13 +/- 1 mus at 77 K and the qua...

journal_title：Biochemistry

authors： Glatz P,Chance B,Vanderkooi JM

更新日期：1979-08-07 00:00:00

abstract：:Di- and triketone inhibitors of (4-hydroxyphenyl)pyruvate dioxygenase (HPPD) are both effective herbicides and therapeutics. The inhibitory activity is used to halt the production of lipophilic redox cofactors in plants and also in humans to prevent accumulation of toxic metabolic byproducts that arise from specific i...

journal_title：Biochemistry

authors： Brownlee JM,Johnson-Winters K,Harrison DH,Moran GR

更新日期：2004-06-01 00:00:00

abstract：:Recent studies have shown that there can be large differences in the stability of double and triple helical nucleic acid complexes, depending on whether RNA or DNA strands are involved. These differences have been attributed to structural differences in the sugar-phosphate backbone of these two polymers. However, sinc...

journal_title：Biochemistry

authors： Wang S,Kool ET

更新日期：1995-03-28 00:00:00

abstract：:The aa(3)-type quinol oxidase from Acidianus ambivalens is a divergent member of the heme-copper oxidases superfamily, namely, concerning the putative channels for intraprotein proton conduction. In this study, we used electrochemically induced FTIR difference spectroscopy to identify residues involved in redox-couple...

journal_title：Biochemistry

authors： Hellwig P,Gomes CM,Teixeira M

更新日期：2003-05-27 00:00:00

abstract：:Understanding the set of rules which dictate how the primary amino acid sequence determines tertiary structure is an unsolved problem in biophysics. If it were possible to simultaneously measure all of the intramolecular distances in a protein (in real time) during a folding reaction, the "second" genetic code problem...

journal_title：Biochemistry

authors： Lillo MP,Szpikowska BK,Mas MT,Sutin JD,Beechem JM

更新日期：1997-09-16 00:00:00

abstract：:The newly described adapter molecule p130 Crk-associated substrate (Cas) has been reported to contribute to cytoskeletal organization through assembly of actin filaments and to be pivotal in embryonic development and in oncogene-mediated transformation. We characterized the regulation of Cas tyrosine phosphorylation i...

journal_title：Biochemistry

authors： Ohmori T,Yatomi Y,Inoue K,Satoh K,Ozaki Y

更新日期：2000-05-16 00:00:00

abstract：:A substrate analogue, 6-(difluoromethyl)tryptophan, was developed and characterized for mechanistic investigation of tryptophanase. The utility of this derivative was based on its ability to partition between fluoride elimination and carbon-carbon bond scission during tryptophan metabolism. The non-enzymatic hydrolysi...

journal_title：Biochemistry

authors： Woolridge EM,Rokita SE

更新日期：1991-02-19 00:00:00

abstract：:RXPA380 (Cbz-PhePsi[PO(2)CH]Pro-Trp-OH) was reported recently as the first highly selective inhibitor of the C-domain of somatic angiotensin-converting enzyme (ACE), able to differentiate the two active sites of somatic ACE by a selectivity factor of more than 3 orders of magnitude. The contribution of each RXPA380 re...

journal_title：Biochemistry

authors： Georgiadis D,Cuniasse P,Cotton J,Yiotakis A,Dive V

更新日期：2004-06-29 00:00:00

abstract：:Thermodynamic studies of bacteriorhodopsin (BR) have been undertaken in order to investigate the factors that stabilize the structure of a membrane protein. The stability of the native, intact protein was compared to that of protein with retinal removed, and/or cleaved in one or two of the loops connecting the transme...

journal_title：Biochemistry

authors： Kahn TW,Sturtevant JM,Engelman DM

更新日期：1992-09-22 00:00:00

abstract：:αB-Crystallin is a chaperone and an anti-apoptotic protein that is strongly expressed in many tissues, including the lens, retina, heart, and kidney. In the human lens, several lysine residues in αB-crystallin are acetylated. We have previously shown that such acetylation is predominant at lysine 92 (K92) and lysine 1...

journal_title：Biochemistry

authors： Nahomi RB,Huang R,Nandi SK,Wang B,Padmanabha S,Santhoshkumar P,Filipek S,Biswas A,Nagaraj RH

更新日期：2013-11-12 00:00:00

abstract：:Alpha-synuclein modulates dopamine homeostasis in dopamine-producing neurons of substantia nigra, partly through regulation of human dopamine transporter (hDAT) activity. To identify the underlying mechanisms, we disrupted the modulation of hDAT activity by wild-type (wt) alpha-synuclein, and its familial Parkinson's ...

journal_title：Biochemistry

authors： Wersinger C,Vernier P,Sidhu A

更新日期：2004-02-10 00:00:00

abstract：:Sterol carrier protein 2 like 2 from Aedes aegypti (AeSCP2L2) plays an important role in lipid transport in mosquitoes for its routine metabolic processes. Repeated unsuccessful attempts to crystallize ligand free SCP2L2 prompted us to undertake nuclear magnetic resonance (NMR) spectroscopy to determine its three-dime...

journal_title：Biochemistry

authors： Singarapu KK,Ahuja A,Potula PR,Ummanni R

更新日期：2016-09-06 00:00:00

abstract：:Trytophanase from Bacillus alvei was resolved from its coenzyme, pyridoxal phosphate, by treatment with cysteine followed by column chromatography. Spectrophotometric titration of apoenzyme with pyridoxal-P showed 1 mol of pyridoxal-P bound per 52,000 g of enzyme. Kinetic analysis of coenzyme binding showed hyperbolic...

journal_title：Biochemistry

authors： Isom HC,DeMoss RD

更新日期：1975-09-23 00:00:00

abstract：:Phthalate dioxygenase from Pseudomonas cepacia contains a mononuclear ferrous center that is strictly required for catalytic oxygen activation. The spectroscopic characterization of this iron site and its ligand interactions has been complicated in the past by interference from a Rieske-type binuclear (2Fe-2S) cluster...

journal_title：Biochemistry

authors： Gassner GT,Ballou DP,Landrum GA,Whittaker JW

更新日期：1993-05-11 00:00:00

abstract：:Sulfite oxidase (SO) catalyzes the physiologically critical conversion of sulfite to sulfate. Enzymatic activity is dependent on the presence of the metal molybdenum complexed with a pyranopterin-dithiolene cofactor termed molybdopterin. Comparison of the amino acid sequences of SOs from a variety of sources has ident...

journal_title：Biochemistry

authors： Qiu JA,Wilson HL,Pushie MJ,Kisker C,George GN,Rajagopalan KV

更新日期：2010-05-11 00:00:00

abstract：:Oxygen binding by Octopus dofleini hemocyanin was examined under very nearly physiological conditions. The effects of pH, ionic composition, temperature, and aggregation were controlled so that the role each plays in modulating oxygen binding can be isolated. There is a very large effect of pH on affinity, the Bohr ef...

journal_title：Biochemistry

authors： Miller KI

更新日期：1985-08-13 00:00:00

abstract：:The structure of PsbQ, one of the three main extrinsic proteins associated with the oxygen-evolving complex (OEC) of higher plants and green algae, is examined by Fourier transform infrared (FTIR) and circular dichroic (CD) spectroscopy and by computational structural prediction methods. This protein, together with tw...

journal_title：Biochemistry

authors： Balsera M,Arellano JB,Gutiérrez JR,Heredia P,Revuelta JL,De Las Rivas J

更新日期：2003-02-04 00:00:00

abstract：:In human glutathione transferase (GST) A1-1, the C-terminal region covers the active site and contributes to substrate binding. This region is flexible, but upon binding of an active-site ligand, it is stabilized as an amphipatic alpha-helix. The stabilization has implications for the catalytic activity of the enzyme....

journal_title：Biochemistry

authors： Gustafsson A,Etahadieh M,Jemth P,Mannervik B

更新日期：1999-12-07 00:00:00

abstract：:In the cyanobacterium Anabaena PCC7120, the precursor to tRNA(Leu) contains a 249-nucleotide group I intron that undergoes efficient self-splicing in vitro. By deleting the 5' and 3' splice sites, this intron has now been converted to an RNA enzyme that uses a guanosine nucleophile to cleave substrate RNAs (S) with mu...

journal_title：Biochemistry

authors： Zaug AJ,Dávila-Aponte JA,Cech TR

更新日期：1994-12-13 00:00:00

abstract：:The effects of ionophore A23187 on the movements of Ca2+, Mg2+, H+ phosphate, and succinate and its effects on energy utilization by mitochondria have been studied as a function of ionophore concentrations. At a low ratio of the compound to mitochondrial protein, below that required for maximal uncoupling, an apparent...

journal_title：Biochemistry

authors： Pfeiffer DR,Hutson SM,Kauffman RF,Lardy HA

更新日期：1976-06-15 00:00:00

abstract：:The SH3 and SH2 domains of hematopoietic cell kinase (Hck) play important roles in substrate targeting. To identify new components of Hck signaling pathways, we identified proteins that bind to the SH3 domain of Hck (Scott et al. (2002) J. Biol. Chem. 277, 28238). One such protein was ELMO1, the mammalian orthologue o...

journal_title：Biochemistry

authors： Yokoyama N,deBakker CD,Zappacosta F,Huddleston MJ,Annan RS,Ravichandran KS,Miller WT

更新日期：2005-06-21 00:00:00

abstract：:Ultraviolet resonance Raman (RR) spectra, with 200- and 218-nm excitation from a H2-shifted quadrupled Nd:YAG laser, are reported for insulin and alpha-lactalbumin in dilute aqueous solution, at pH values known to produce differences in the exposure of the aromatic residues to solvent. At 200 nm, the spectra are domin...

journal_title：Biochemistry

authors： Rava RP,Spiro TG

更新日期：1985-04-09 00:00:00

abstract：:Inosine 5'-monophosphate (IMP) cyclohydrolase catalyzes the cyclization of 5-formaminoimidazole-4-carboxamide ribonucleotide (FAICAR) to IMP in the final step of de novo purine biosynthesis. Two major types of this enzyme have been discovered to date: PurH in Bacteria and Eukarya and PurO in Archaea. The structure of ...

journal_title：Biochemistry

authors： Kang YN,Tran A,White RH,Ealick SE

更新日期：2007-05-01 00:00:00

abstract：:Mild acid hydrolysis of Hafnia alvei strain 2 lipopolysaccharide released no O-specific polysaccharide but instead gave a monomeric octasaccharide repeating unit with N-acetylneuraminic acid as the reducing terminus. In addition, a dimer of the octasaccharide repeating unit, and also a decasaccharide composed of a fra...

journal_title：Biochemistry

authors： Gamian A,Romanowska E,Dabrowski U,Dabrowski J

更新日期：1991-05-21 00:00:00

abstract：:The Escherichia coli L-arabinose-binding protein (ABP) participates as a specific receptor in the transport of L-arabinose, D-fucose, and D-galactose through the periplasmic space. The wild-type protein binds L-arabinose about 40 times more strongly than D-fucose. A mutation of the protein at position 108 (Met-->Leu) ...

journal_title：Biochemistry

authors： Zacharias M,Straatsma TP,McCammon JA,Quiocho FA

更新日期：1993-07-27 00:00:00

abstract：:S-Adenosylmethionine (SAM+) serves as the principal methylating agent in biological systems, but the thermodynamic basis of its reactivity does not seem to have been clearly established. Here, we show that methionine, methanol, and H+ combine to form S-methylmethionine (SMM+) with a temperature-independent equilibrium...

journal_title：Biochemistry

authors： Lewis CA Jr,Wolfenden R

更新日期：2018-07-03 00:00:00