Solution Nuclear Magnetic Resonance Studies of Sterol Carrier Protein 2 Like 2 (SCP2L2) Reveal the Insecticide Specific Structural Characteristics of SCP2 Proteins in Aedes aegypti Mosquitoes.

abstract：:Photosystem II, the multisubunit protein complex that oxidizes water to O2, requires the inorganic cofactors Ca2+ and Cl- to exhibit optimal activity. Chloride can be replaced functionally by a small number of anionic cofactors (Br-, NO3-, NO2-, I-), but among these anions, only Br- is capable of restoring rates of ox...

journal_title：Biochemistry

authors： Wincencjusz H,Yocum CF,van Gorkom HJ

更新日期：1999-03-23 00:00:00

abstract：:Although the short-chain dehydrogenase/reductase (SDR) superfamily contains a very large number of members defined in annotated databases and by biochemical and structural studies, very few SDR enzymes have been identified that have a homologous partner catalyzing the same reaction but with an opposite stereospecifici...

journal_title：Biochemistry

authors： Clark DD,Ensign SA

更新日期：2002-02-26 00:00:00

abstract：:Imino proton resonances in the downfield region (10-14 ppm) of the 500-MHz 1H NMR spectrum of Torulopsis utilis 5S RNA are identified (A X U, G X C, or G X U) and assigned to base pairs in helices I, IV, and V via analysis of homonuclear Overhauser enhancements (NOE) from intact T. utilis 5S RNA, its RNase T1 and RNas...

journal_title：Biochemistry

authors： Chen SM,Marshall AG

更新日期：1986-09-09 00:00:00

abstract：:The structure of cytochrome f includes an internal chain of five water molecules and six hydrogen-bonding side chains, which are conserved throughout the phylogenetic range of photosynthetic organisms from higher plants, algae, and cyanobacteria. The in vivo electron transfer capability of Chlamydomonas reinhardtii cy...

journal_title：Biochemistry

authors： Sainz G,Carrell CJ,Ponamarev MV,Soriano GM,Cramer WA,Smith JL

更新日期：2000-08-08 00:00:00

abstract：:Fluoroaluminate and fluoroberyllate are potent inhibitors of the ATPase activity of myosin. Inhibition requires the presence of ADP, and much evidence has accumulated to suggest that the tetrahedral fluoroaluminate and fluoroberyllate ions act as phosphate analogues, binding with high affinity at the active site in th...

journal_title：Biochemistry

authors： Henry GD,Maruta S,Ikebe M,Sykes BD

更新日期：1993-10-05 00:00:00

abstract：:The surface electrical charge density and the deformability of nucleosomal arrays have been characterized by quantitative agarose gel electrophoresis. Monodisperse linear DNA (2.5-3.3 kbp) was reconstituted with histone octamers into either saturated (approximately 1 nucleosome/200-bp DNA) or subsaturated (< 1 nucleos...

journal_title：Biochemistry

authors： Fletcher TM,Krishnan U,Serwer P,Hansen JC

更新日期：1994-03-01 00:00:00

abstract：:Direct chemical titrations of reduced, purified cytochrome aa3 were monitored at 604 nm. Anaerobic oxidation by potassium ferricyanide or 1,1'-bis(hydroxymethyl)-ferricinium ion was compared with the natural substrate, molecular oxygen. The four electrons from reduced cytochrome aa3 were donated to one oxygen molecule...

journal_title：Biochemistry

authors： Schroedl NA,Hartzell CR

更新日期：1977-04-05 00:00:00

abstract：:The heme-regulated inhibitor (HRI) of protein synthesis becomes activated in rabbit reticulocyte lysates in response to a variety of conditions including heme-deficiency, addition of oxidants, and heat shock. Activated HRI inhibits translation by catalyzing the phosphorylation of the alpha-subunit of eukaryotic initia...

journal_title：Biochemistry

authors： Matts RL,Hurst R,Xu Z

更新日期：1993-07-27 00:00:00

abstract：:The molecular structure of ammonium deoxycytidylyl-(3'-5')-deoxyguanosine, crystallized from aqueous acetone near pH 4, was determined for X-ray diffraction data. The crystals were tetragonal, space group P43212 with a = b = 11.078 (1) A and c = 45.826 (4) A. The structure was solved by tangent expansion of phases bas...

journal_title：Biochemistry

authors： Cruse WB,Egert E,Kennard O,Sala GB,Salisbury SA,Viswamitra MA

更新日期：1983-04-12 00:00:00

abstract：:The nitrite anion is known to oxidize and degrade hemoglobin (Hb). Recent literature reports suggest a nitrite reductase activity for Hb, converting nitrite into nitric oxide. Surprisingly, no structural information about Hb-nitrite interactions has been reported. We have determined the crystal structure of the ferric...

journal_title：Biochemistry

authors： Yi J,Safo MK,Richter-Addo GB

更新日期：2008-08-12 00:00:00

abstract：:We describe the synthesis of bordifluoropyrromethene (BODIPY), fluorescein, and related fluorescent derivatives of the beta-adrenergic ligand CGP 12177. With these probes we screened insect (Sf9) cells stably transformed with the human beta 2-adrenoceptor gene and expressing (2-3.5) x 10(5) human beta 2-adrenoceptors ...

journal_title：Biochemistry

authors： Heithier H,Hallmann D,Boege F,Reiländer H,Dees C,Jaeggi KA,Arndt-Jovin D,Jovin TM,Helmreich EJ

更新日期：1994-08-09 00:00:00

abstract：:The aggregation of the microtubule-associated protein, tau, into amyloid fibrils is a hallmark of neurodegenerative diseases such as the tauopathies and Alzheimer's disease. Since monomeric tau is an intrinsically disordered protein and the polymeric fibrils possess an ordered cross-β core, the aggregation process is ...

journal_title：Biochemistry

authors： Ramachandran G,Milán-Garcés EA,Udgaonkar JB,Puranik M

更新日期：2014-10-21 00:00:00

abstract：:We have sought to determine whether insulin can promote cell survival and protect Chinese hamster ovary (CHO) cells from apoptosis induced by serum starvation. Low concentrations of insulin were antiapoptotic for cells overexpressing wild-type insulin receptors but not in cells transfected with kinase-defective insuli...

journal_title：Biochemistry

authors： Lee-Kwon W,Park D,Baskar PV,Kole S,Bernier M

更新日期：1998-11-10 00:00:00

abstract：:The EcoRV DNA-(adenine-N6)-methyltransferase (MTase) recognizes GATATC sequences and modifies the first adenine residue within this site. Parts of its DNA interface show high sequence homology to DNA MTases of the dam family which recognize and modify GATC sequences. A phylogenetic analysis of M.EcoRV and dam-MTases s...

journal_title：Biochemistry

authors： Beck C,Cranz S,Solmaz M,Roth M,Jeltsch A

更新日期：2001-09-18 00:00:00

abstract：:Structural properties of heparan sulfate preparations from hog mucosa and beef lung sources were obtained by application of Smith degradation and nitrous acid reactions. Products formed by these reactions indicated that most of the iduronic acid present in these mucopolysaccharides is ester sulfated, whereas N-sulfate...

journal_title：Biochemistry

authors： Cifonelli JA,King JA

更新日期：1977-05-17 00:00:00

abstract：:Recently, we synthesized the first individual β,γ-CHX-dGTP diastereomers [(R)- or (S)-CHX, where X is F or Cl] and determined their structures in ternary complexes with DNA polymerase β (pol β). We now report stereospecificity by pol β on the mixed β,γ-CHX diastereomer pairs using nuclear magnetic resonance and on the...

journal_title：Biochemistry

authors： Oertell K,Wu Y,Zakharova VM,Kashemirov BA,Shock DD,Beard WA,Wilson SH,McKenna CE,Goodman MF

更新日期：2012-10-30 00:00:00

abstract：:The complex of ribonuclease A (RNase A) with uridine vanadate (U-V), a transition-state analogue, has been studied with 51V and proton NMR spectroscopy in solution and by neutron diffraction in the crystalline state. Upon the addition of aliquots of U-V at pH 6.6, the C epsilon-H resonances of the two active-site hist...

journal_title：Biochemistry

authors： Borah B,Chen CW,Egan W,Miller M,Wlodawer A,Cohen JS

更新日期：1985-04-09 00:00:00

abstract：:An allelic variant of the ouabain-insensitive rat kidney Na+,K(+)-ATPase alpha 1-isoform was identified by chance in a cDNA library. The variant differed from the wild-type rat kidney Na+,K(+)-ATPase by a single G to C base substitution in the cDNA, which on the amino acid level gave rise to a glutamine in place of th...

journal_title：Biochemistry

authors： Vilsen B

更新日期：1993-12-07 00:00:00

abstract：:The gene 5 protein (g5p) encoded by filamentous Ff phages is an ssDNA-binding protein, which binds to and sequesters the nascent ssDNA phage genome in the process of phage morphogenesis. The g5p also binds with high affinity to DNA and RNA sequences that form G-quadruplex structures. However, sequences that would form...

journal_title：Biochemistry

authors： Wen JD,Gray DM

更新日期：2004-03-09 00:00:00

abstract：:The 1H resonances of the high-potential [4Fe-4S]2+ ferredoxin from Chromatium vinosum have been assigned through conventional sequential methodology applied to 2D NMR spectra. Almost 80% of the residues were identified using standard 2D COSY, HOHAHA, and NOESY pulse sequences. These residues correspond to four segment...

journal_title：Biochemistry

authors： Gaillard J,Albrand JP,Moulis JM,Wemmer DE

更新日期：1992-06-23 00:00:00

abstract：:The stereoselectivity of the adenosine cyclic 3',5'-phosphate (cAMP) binding sites on the regulatory subunit of the type II bovine cardiac muscle cAMP-dependent protein kinase was investigated by examining the interactions of (Rp)- and (Sp)-adenosine cyclic 3',5'-phosphorothioates (cAMPS) with these sites. While activ...

journal_title：Biochemistry

authors： O'Brian CA,Roczniak SO,Bramson HN,Baraniak J,Stec WJ,Kaiser ET

更新日期：1982-08-31 00:00:00

abstract：:Polyphosphate glucokinase from Mycobacterium tuberculosis catalyzes the phosphorylation of glucose using inorganic polyphosphates [poly(P)] or ATP. The steady-state kinetic mechanisms of the poly(P)- and ATP-dependent glucokinase reactions were investigated using initial velocity, product inhibition, and dead-end inhi...

journal_title：Biochemistry

authors： Hsieh PC,Kowalczyk TH,Phillips NF

更新日期：1996-07-30 00:00:00

abstract：:Both activities of the aspartokinase--homoserine I (AK-HSD) of Escherichia coli are inhibited by threonine. Careful threonine binding studies have now been done which have allowed us to distinguish the various effects of threonine on the enzyme. The ultrafiltration technique of H. Paulus ((1969) Anal. Biochem. 32, 101...

journal_title：Biochemistry

authors： Bearer CF,Neet KE

更新日期：1978-08-22 00:00:00

abstract：:The antibiotic bicyclomycin inhibits rho-dependent termination processes by interfering with RNA translocation by preventing RNA binding at the translocation site or by uncoupling the translocation process from ATP hydrolysis. Previous studies have shown that bicyclomycin binds near the ATP hydrolysis pocket on rho. T...

journal_title：Biochemistry

authors： Vincent F,Openshaw M,Trautwein M,Gaskell SJ,Kohn H,Widger WR

更新日期：2000-08-08 00:00:00

abstract：:The structural consequences of specific base sequences in DNA can exert a strong influence on the function of DNA. It has previously been reported that the presence of multiple B-Z conformational junctions in constructed DNA oligomers results in unusually enhanced electrophoretic gel mobilities of these oligomers [Win...

journal_title：Biochemistry

authors： Lu M,Guo Q,Kallenbach NR,Sheardy RD

更新日期：1992-05-19 00:00:00

abstract：:We identified amino acid residues important for activity of sod2, the Na+/H+ antiporter of Schizosaccharomyces pombe. We mutated all eight His residues of sod2 into Arg. Only His367-->Arg affected function and resulted in complete inability of sod2 to allow growth of S. pombe in LiCl-containing medium. Mutant S. pombe...

journal_title：Biochemistry

authors： Dibrov P,Young PG,Fliegel L

更新日期：1998-06-09 00:00:00

abstract：:Synthetic nonviral vector systems are attractive because of their apparent simplicity of preparation and use. However, there are many barriers to success at the moment, including the formulation of uniform and reproducible particles of transfection competent, condensed nucleic acids such as plasmid DNA (pDNA). For thi...

journal_title：Biochemistry

authors： Tecle M,Preuss M,Miller AD

更新日期：2003-09-09 00:00:00

abstract：:Site-directed mutagenesis and high-resolution two-dimensional (2D) proton nuclear magnetic resonance (NMR) were used to probe the structural and functional roles of a highly conserved residue, Asp-49, in the interfacial catalysis by bovine pancreatic phospholipase A2 (PLA2, overexpressed in Escherichia coli). Accordin...

journal_title：Biochemistry

authors： Li Y,Yu BZ,Zhu H,Jain MK,Tsai MD

更新日期：1994-12-13 00:00:00

abstract：:The GlmU protein is a bifunctional enzyme with both acetyltransferase and uridylyltransferase (pyrophosphorylase) activities which catalyzes the transformation of glucosamine-1-P, UTP, and acetyl-CoA to UDP-N-acetylglucosamine [Mengin-Lecreulx, D., & van Heijenoort, J. (1994) J. Bacteriol. 176, 5788-5795], a fundament...

journal_title：Biochemistry

authors： Gehring AM,Lees WJ,Mindiola DJ,Walsh CT,Brown ED

更新日期：1996-01-16 00:00:00

abstract：:Thromboxane A(2) receptor (TP receptor), a prostanoid receptor, belongs to the G protein-coupled receptor family, composed of three intracellular loops and three extracellular loops connecting seven transmembrane helices. The highly conserved extracellular domains of the prostanoid receptors were found in the second e...

journal_title：Biochemistry

authors： Ruan KH,So SP,Wu J,Li D,Huang A,Kung J

更新日期：2001-01-09 00:00:00