Functional analysis of amino acid residues essential for activity in the Na+/H+ exchanger of fission yeast.

abstract：:Resonance Raman spectra of squid rhodopsin have been obtained under a variety of temperature and illumination conditions. The data have been characterized in terms of spectral contributions from squid rhodopsin, isorhodopsin, bathorhodopsin, lumirhodopsin, mesorhodopsin, P-465, and acid metarhodopsin. The results are ...

journal_title：Biochemistry

authors： Sulkes M,Lewis A,Marcus MA

更新日期：1978-10-31 00:00:00

abstract：:Theory for enzyme-catalyzed reactions is developed for the dependence on product concentration of deuterium isotope effects on V and V/K. Generally, a product that decreases the off-rate for a second product to zero causes the isotope effect on V/K to decrease to DKeq and that on V to decrease to a value between 1 and...

journal_title：Biochemistry

authors： Cook PF,Yoon MY,Hara S,McClure GD Jr

更新日期：1993-02-23 00:00:00

abstract：:The C-terminal domain (CT) of apolipoprotein E (apoE), a critical protein involved in cholesterol transport in the plasma and brain, plays an important role in high-affinity lipoprotein binding. Although high-resolution structural information is available for the N-terminal domain of apoE, the structural organization ...

journal_title：Biochemistry

authors： Patel AB,Khumsupan P,Narayanaswami V

更新日期：2010-03-02 00:00:00

abstract：:Hemopexin is a plasma glycoprotein that has two structural domains (I and II) and binds and transports heme particularly to liver cells. Differential scanning calorimetry (DSC) studies show that hemopexin is largely stabilized by heme, which binds exclusively to domain I. The melting temperature (Tm) of heme-hemopexin...

journal_title：Biochemistry

authors： Wu ML,Morgan WT

更新日期：1993-07-20 00:00:00

abstract：:Sf9 cells infected with a recombinant baculovirus containing the gene for human prorenin were cultured in the presence of [3H]mannose. In vivo labeled prorenin was isolated by immunoprecipitation from the culture medium and digested with Pronase. The oligosaccharide structures on the resulting glycopeptides were analy...

journal_title：Biochemistry

authors： Aeed PA,Elhammer AP

更新日期：1994-07-26 00:00:00

abstract：:alpha5beta1 integrin can occupy several distinct conformational states which support different strengths of binding to fibronectin [García, A. J., et al. (1998) J. Biol. Chem. 273, 34710-34715]. Using a model system in which specific activating monoclonal antibodies were used to achieve uniform activated states, the b...

journal_title：Biochemistry

authors： García AJ,Schwarzbauer JE,Boettiger D

更新日期：2002-07-23 00:00:00

abstract：:Alignment of more than 20 deduced sequences for mevalonate diphosphate decarboxylase (MDD) indicates that serines 34, 36, 120,121, 153, and 155 are invariant residues that map within a proposed interdomain active site cleft. To test possible active site roles for these invariant serines, each has been mutated to alani...

journal_title：Biochemistry

authors： Krepkiy DV,Miziorko HM

更新日期：2005-02-22 00:00:00

abstract：:It has been found that centrifugation of (ethylenediamine)tetraacetic acid dissociated L-cell polyribosomes at 25 degrees C through preformed CS2SO4 density gradients containing 15% dimethyl sulfoxide resolves them into two distinct classes of particles that contain ribosomal and messenger RNA, respectively. Ribosomal...

journal_title：Biochemistry

authors： Greenberg JR

更新日期：1976-08-10 00:00:00

abstract：:1,3-15N-Labeled uracil was synthesized chemically and used to prepare labeled Escherichia coli tRNA(Val) biosynthetically. 500-MHz measurements of 15N and proton chemical shift were obtained, for all uridine and uridine-related bases, by heteronuclear multiple-quantum coherence spectroscopy. All the uracil NH group re...

journal_title：Biochemistry

authors： Choi BS,Redfield AG

更新日期：1992-12-29 00:00:00

abstract：:Peptides corresponding to portions of curaremimetic neurotoxin loop 2 and to a structurally similar segment of rabies virus glycoprotein were synthetically modified in order to gain information on structure-function relationships of neurotoxin loop 2 interactions with the acetylcholine receptor. Binding of synthetic p...

journal_title：Biochemistry

authors： Lentz TL

更新日期：1991-11-12 00:00:00

abstract：:Polyclonal antibody to mitochondrial P-450c27/25 reacted with two proteins of apparent molecular masses of 52 kilodaltons (kDa) and 50 kDa from the female rat liver mitochondrial proteins bound to an omega-octylaminoagarose column. The two proteins were purified to greater than 85% homogeneity by DEAE-Sephacel and hyd...

journal_title：Biochemistry

authors： Addya S,Zheng YM,Shayiq RM,Fan JY,Avadhani NG

更新日期：1991-08-27 00:00:00

abstract：:We used a combination of optical and calorimetric techniques to investigate the incorporation of deoxythymidine --> deoxyuridine (dT --> dU) substitutions in the duplex and third strand of the parallel intramolecular triplex d(A(7)C(5)T(7)C(5)T(7)) (ATT). UV and differential scanning calorimetry melting experiments sh...

journal_title：Biochemistry

authors： Soto AM,Rentzeperis D,Shikiya R,Alonso M,Marky LA

更新日期：2006-03-07 00:00:00

abstract：:Chlorophyll a fluorescence emission is widely used as a noninvasive measure of a number of parameters related to photosynthetic efficiency in oxygenic photosynthetic organisms. The most important component for the estimation of photochemistry is the relative increase in fluorescence yield between dark-adapted samples ...

journal_title：Biochemistry

authors： Vasil'ev S,Bruce D

更新日期：1998-08-04 00:00:00

abstract：:Site-directed spin labeling (SDSL), the site-specific incorporation of nitroxide spin-labels into a protein, has allowed us to investigate ligand-induced conformational changes in the ligand-binding domain of human estrogen receptor alpha (hERalpha-LBD). EPR (electron paramagnetic resonance) spectroscopy of the nitrox...

journal_title：Biochemistry

authors： Hurth KM,Nilges MJ,Carlson KE,Tamrazi A,Belford RL,Katzenellenbogen JA

更新日期：2004-02-24 00:00:00

abstract：:The gamma subunit of the retinal cGMP phosphodiesterase (gammaPDE) acts as an inhibitor of phosphodiesterase (PDE) catalytic activity and mediates enzyme regulation by the alpha subunit of the GTP-binding protein transducin (alphaT). In this work, we describe a full length, doubly point-mutated gamma subunit, C68S, Y8...

journal_title：Biochemistry

authors： Berger AL,Cerione RA,Erickson JW

更新日期：1999-01-26 00:00:00

abstract：:Succinyl-CoA:3-oxoacid coenzyme A transferase (EC 2.8.3.5), which catalyzes the reversible conversion of succinyl-CoA and acetoacetate into acetoacetyl-CoA and succinate through a covalent enzyme thiol ester intermediate, E-CoA, utilizes binding energy from noncovalent interactions with CoA to bring about an increase ...

journal_title：Biochemistry

authors： Whitty A,Fierke CA,Jencks WP

更新日期：1995-09-19 00:00:00

abstract：:We reported previously that cdc2 kinase decreased the microtubule-stabilizing ability of a major HeLa cell microtubule-associated protein, MAP4, by phosphorylation in vitro [Ookata, K., et al. (1995) J. Cell Biol. 128, 849-862]. An important question raised by this study is whether MAP4 is indeed phosphorylated by cdc...

journal_title：Biochemistry

authors： Ookata K,Hisanaga S,Sugita M,Okuyama A,Murofushi H,Kitazawa H,Chari S,Bulinski JC,Kishimoto T

更新日期：1997-12-16 00:00:00

abstract：:Flavohemoglobins (flavoHbs) are enzymes that operate primarily as nitric oxide dioxygenases and shuttle thereby electrons among NAD(P)H, FAD, heme, and a ligated redox-active substrate such as O(2). They function in the bacterial defense against nitrosative stress and are therefore considered as targets for new antibi...

journal_title：Biochemistry

authors： El Hammi E,Warkentin E,Demmer U,Limam F,Marzouki NM,Ermler U,Baciou L

更新日期：2011-02-22 00:00:00

abstract：:alpha 1-Adrenergic receptor probes, which can be radioiodinated to yield high specific activity radioligands, have been synthesized and characterized. 2-[4-(4-Amino-benzoyl)piperazin-1-yl]-4-amino-6,7-dimethoxyquin azoline (CP63,155), an arylamine analogue of the selective alpha 1-adrenergic antagonist prazosin, and i...

journal_title：Biochemistry

authors： Seidman CE,Hess HJ,Homcy CJ,Graham RM

更新日期：1984-07-31 00:00:00

abstract：:Comparative sequence analysis has successfully predicted secondary structure and tertiary interactions in ribosomal and other RNAs. Experiments presented here ask whether the scope of comparative sequence-based predictions can be extended to specific interactions between proteins and RNA, using as a system the well-ch...

journal_title：Biochemistry

authors： GuhaThakurta D,Draper DE

更新日期：1999-03-23 00:00:00

abstract：:The mechanism of the slow refolding reactions of four different pancreatic ribonucleases from ox, sheep, red deer, and roe deer has been investigated. Refolding kinetics of these proteins were very similar. In particular, a native-like intermediate, IN, was shown to be populated on the slow refolding pathway of all ri...

journal_title：Biochemistry

authors： Krebs H,Schmid FX,Jaenicke R

更新日期：1985-07-16 00:00:00

abstract：:Cyclic AMP receptor protein is involved in the regulation of more than 20 genes. A step in the mechanism of activation of transcription is to induce a significant bending of the DNA upon complex formation between specific DNA and the protein. The induced DNA bending and a structure of the protein-DNA complex were stud...

journal_title：Biochemistry

authors： Heyduk T,Lee JC

更新日期：1992-06-09 00:00:00

abstract：:In our previous papers, seven structural genes (NQO1-7) of the energy-transducing NADH-quinone (Q) oxidoreductase of Paracoccus denitrificans were characterized [Xu, X., Matsuno-Yagi, A., & Yagi, T. (1991a) Biochemistry 30, 8678-8684; (1991b) Biochemistry 30, 6422-6428; (1992a) Biochemistry 31, 6925-6932; (1992b) Arch...

journal_title：Biochemistry

authors： Xu X,Matsuno-Yagi A,Yagi T

更新日期：1993-01-26 00:00:00

abstract：:Amyloid deposition of human islet amyloid polypeptide (hIAPP) in the islets of Langerhans is closely associated with the pathogenesis of type II diabetes mellitus. Despite substantial evidence linking amyloidogenic hIAPP to loss of β-cell mass and decreased pancreatic function, the molecular mechanism of hIAPP cytotox...

journal_title：Biochemistry

authors： Sasahara K,Morigaki K,Okazaki T,Hamada D

更新日期：2012-09-04 00:00:00

abstract：:RXPA380 (Cbz-PhePsi[PO(2)CH]Pro-Trp-OH) was reported recently as the first highly selective inhibitor of the C-domain of somatic angiotensin-converting enzyme (ACE), able to differentiate the two active sites of somatic ACE by a selectivity factor of more than 3 orders of magnitude. The contribution of each RXPA380 re...

journal_title：Biochemistry

authors： Georgiadis D,Cuniasse P,Cotton J,Yiotakis A,Dive V

更新日期：2004-06-29 00:00:00

abstract：:Three methods, fluorescence anisotropy of rhodamine-labeled profilin, intrinsic fluorescence and nucleotide exchange, give the same affinity, Kd = 0.1 microM, for Acanthamoeba profilins binding amoeba actin monomers with bound Mg-ATP. Replacement of serine 38 with cysteine created a unique site where labeling with rho...

journal_title：Biochemistry

authors： Vinson VK,De La Cruz EM,Higgs HN,Pollard TD

更新日期：1998-08-04 00:00:00

abstract：:The bacterial DNA cytosine methyltransferase M.HhaI sequence-specifically modifies DNA in an S-adenosylmethionine dependent reaction. The enzyme stabilizes the target cytosine (GCGC) into an extrahelical position, with a concomitant large movement of an active site loop involving residues 80-99. We used multidimension...

journal_title：Biochemistry

authors： Zhou H,Shatz W,Purdy MM,Fera N,Dahlquist FW,Reich NO

更新日期：2007-06-19 00:00:00

abstract：:Designing subtype-selective agonists for neuronal nicotinic acetylcholine receptors is a challenging and significant goal aided by intricate knowledge of each subtype's binding patterns. We previously reported that in α6β2 receptors, acetylcholine makes a functional cation-π interaction with Trp149, but nicotine and T...

journal_title：Biochemistry

authors： Post MR,Lester HA,Dougherty DA

更新日期：2017-04-04 00:00:00

abstract：:Domains are in general less stable than the corresponding full-length proteins. Human utrophin tandem calponin-homology (CH) domain seems to be an exception. Reversible, equilibrium denaturant melts indicate that the isolated C-terminal domain (CH2) is thermodynamically more stable than the tandem CH domain. Thermal m...

journal_title：Biochemistry

authors： Bandi S,Singh SM,Mallela KM

更新日期：2014-04-15 00:00:00

abstract：:The phytocyanins are a family of plant cupredoxins that have been subdivided into the stellacyanins, plantacyanins, and uclacyanins. All of these proteins possess the typical type 1 His(2)Cys equatorial ligand set at their mononuclear copper sites, but the stellacyanins have an axial Gln ligand in place of the weakly ...

journal_title：Biochemistry

authors： Harrison MD,Yanagisawa S,Dennison C

更新日期：2005-03-01 00:00:00