Abstract：
:1,3-15N-Labeled uracil was synthesized chemically and used to prepare labeled Escherichia coli tRNA(Val) biosynthetically. 500-MHz measurements of 15N and proton chemical shift were obtained, for all uridine and uridine-related bases, by heteronuclear multiple-quantum coherence spectroscopy. All the uracil NH group resonances were assigned and were in agreement with previous proton-only assignments. The temperature dependence of intensities of resonances was used to infer the relative stability of parts of the molecule. The acceptor stem was the least thermally stable structural feature, while the anticodon and T loop were relatively more stable.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Choi BS,Redfield AGdoi
10.1021/bi00166a013subject
Has Abstractpub_date
1992-12-29 00:00:00pages
12799-802issue
51eissn
0006-2960issn
1520-4995journal_volume
31pub_type
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