Abstract:
:Some ascidians sequester vanadium and other metal ions that are bound and transported in higher organisms by transferrin. The ascidian Ciona intestinalis has a monolobal transferrin (nicatransferrin) in its plasma. The binding of vanadium(V) to nicatransferrin was investigated by using isothermal titration calorimetry and UV-vis spectroscopy, in the presence and absence of NaHCO(3), and was compared with human serum transferrin. Nicatransferrin and serum transferrin bind V(V) with similar strengths [K = (2.0 +/- 0.6) x 10(5) M(-1) for nicatransferrin]; however, nicatransferrin requires a synergistic anion for V(V) binding, whereas serum transferrin does not. Spectroscopy supports a different kind of coordination site.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Gaffney JP,Valentine AMdoi
10.1021/bi901630jsubject
Has Abstractpub_date
2009-12-15 00:00:00pages
11609-11issue
49eissn
0006-2960issn
1520-4995journal_volume
48pub_type
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