Catalysis of thiol/disulfide exchange: single-turnover reduction of protein disulfide-isomerase by glutathione and catalysis of peptide disulfide reduction.

abstract：:Osteopontin (OPN) is an acidic hydrophilic glycophosphoprotein that was first identified as a major sialoprotein in bones. It functions as a cell attachment protein displaying a RGD cell adhesion sequence and as a cytokine that signals through integrin and CD44 cell adhesion molecules. OPN is also implicated in human ...

journal_title：Biochemistry

authors： Platzer G,Schedlbauer A,Chemelli A,Ozdowy P,Coudevylle N,Auer R,Kontaxis G,Hartl M,Miles AJ,Wallace BA,Glatter O,Bister K,Konrat R

更新日期：2011-07-12 00:00:00

abstract：:The gene encoding enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system from an Escherichia coli enzyme I mutant was cloned and sequenced. The mutation was shown to be a guanine to adenine transition resulting in an altered protein in which glycine-338 was replaced by aspartic acid. The enzyme I structur...

journal_title：Biochemistry

authors： Seok YJ,Lee BR,Gazdar C,Svenson I,Yadla N,Peterkofsky A

更新日期：1996-01-09 00:00:00

abstract：:A crystal structure of human fibrinogen has been determined at approximately 3.3 A resolution. The protein was purified from human blood plasma, first by a cold ethanol precipitation procedure and then by stepwise chromatography on DEAE-cellulose. A product was obtained that was homogeneous on SDS-polyacrylamide gels....

journal_title：Biochemistry

authors： Kollman JM,Pandi L,Sawaya MR,Riley M,Doolittle RF

更新日期：2009-05-12 00:00:00

abstract：:The specificity of horse liver alcohol dehydrogenase for cyclohexanol and its 3-methyl derivatives was investigated by stopped-flow and initial velocity kinetic studies. The (1S,3S)-3-methylcyclohexanol was 7 times more reactive (V/Km) than cyclohexanol, whereas the (1R,3R)-3-methylcyclohexanol was at least 1000 times...

journal_title：Biochemistry

authors： Lee KM,Dahlhauser KF,Plapp BV

更新日期：1988-05-03 00:00:00

abstract：:12-Iodo-cis-9-octadecenoic acid (12-IODE) is a time-dependent, irreversible inactivator of soybean lipoxygenase 1. The rate of inactivation is independent of 12-IODE concentration above 20 microM and is half-maximal at about 4 microM. Inactivation by 12-IODE requires lipid hydroperoxide, which must be present even aft...

journal_title：Biochemistry

authors： Rotenberg SA,Grandizio AM,Selzer AT,Clapp CH

更新日期：1988-11-29 00:00:00

abstract：:Treatment of cells with the enediyne C-1027 is highly efficient at inducing single- and double-strand DNA breaks. This agent is highly cytotoxic when used at picomolar levels over a period of days. For this study, C-1027 has been used at higher levels for a much shorter time period to look at early cellular responses ...

journal_title：Biochemistry

authors： Liu JS,Kuo SR,Yin X,Beerman TA,Melendy T

更新日期：2001-12-04 00:00:00

abstract：:Ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase (Rubisco) is a globally significant biocatalyst that facilitates the removal and sequestration of CO2 from the biosphere. Rubisco-catalyzed CO2 reduction thus provides virtually all of the organic carbon utilized by living organisms. Despite catalyzing the rate-li...

journal_title：Biochemistry

authors： Satagopan S,Scott SS,Smith TG,Tabita FR

更新日期：2009-09-29 00:00:00

abstract：:The membrane topology model of the Na(+)-citrate transporter CitS of Klebsiella pneumoniae shows a core of two homologous domains with opposite orientation in the membrane and each containing a so-called reentrant loop. A split version of CitS was constructed to study domain interactions and proximity relationships of...

journal_title：Biochemistry

authors： Dobrowolski A,Fusetti F,Lolkema JS

更新日期：2010-06-01 00:00:00

abstract：:Sequence-specific DNA binding proteins generally consist of more than two DNA-contacting regions to ensure the selectivity of recognition. The multiple DNA binding modules are connected either through the covalent linker or through the noncovalent dimerization domain. We have compared the DNA binding of peptide dimers...

journal_title：Biochemistry

authors： Aizawa Y,Sugiura Y,Morii T

更新日期：1999-02-02 00:00:00

abstract：:The interaction of novel, tetrafunctional bisplatinum compounds with DNA was investigated. These compounds have bis(vicinal 1,2-diamines) as ligand. The reactions' efficiency, types of cross-links, alterations of the global DNA structure, and sequence selectivity differ significantly from the corresponding features of...

journal_title：Biochemistry

authors： Büning H,Altman J,Beck W,Zorbas H

更新日期：1997-09-23 00:00:00

abstract：:The specific effect of the binding of IF2 and initiator fMET-tRNA(fMet) on Escherichia coli 16S rRNA has been probed by phosphate alkylation with ethylnitrosourea. The results show that IF2 does not significantly shield portions of 16S rRNA but induces both reductions and enhancements of reactivity scattered in the en...

journal_title：Biochemistry

authors： Wakao H,Romby P,Laalami S,Ebel JP,Ehresmann C,Ehresmann B

更新日期：1990-09-04 00:00:00

abstract：:Naturally occurring interruptions in nonfibrillar collagen play key roles in molecular flexibility, collagen degradation, and ligand binding. The structural feature of the interruption sequences and the molecular basis for their functions have not been well studied. Here, we focused on a G5G type natural interruption ...

journal_title：Biochemistry

authors： Xu T,Zhou CZ,Xiao J,Liu J

更新日期：2018-02-20 00:00:00

abstract：:Fourier transform infrared (FTIR) spectroscopy was employed to examine the thermal denaturation of the Fe(III), Fe(II), and Fe(II)-CO forms of cytochrome c peroxidase and horseradish peroxidase in phosphate buffer at pD 7.0. The amide I' regions of the deconvolved spectra are consistent with predominantly alpha-helica...

journal_title：Biochemistry

authors： Holzbaur IE,English AM,Ismail AA

更新日期：1996-04-30 00:00:00

abstract：:The aminoacylase-1/metallopeptidase 20 (Acy1/M20) family features several l-aminoacylases useful in biocatalysis. Mammalian Acy1, in particular, has been applied in racemic resolution and reverse hydrolysis. Despite recent advances in our understanding of the active site architecture and functioning, determinants of A...

journal_title：Biochemistry

authors： Lindner HA,Alary A,Wilke M,Sulea T

更新日期：2008-04-08 00:00:00

abstract：:[125I]Calmodulin binding to isolated rat adipocyte plasma membranes has been characterized, and the calmodulin binding proteins associated with the membrane have been identified by use of the photoaffinity cross-linker N-hydroxysuccinimidyl 4-azidobenzoate. Total binding of [125I]calmodulin to plasma membranes was ass...

journal_title：Biochemistry

authors： Goewert RR,Landt M,McDonald JM

更新日期：1982-10-12 00:00:00

abstract：:The enthalpies of transfer of hen egg white lysozyme from water to aqueous solutions of 1-propanol were determined by isothermal flow calorimetry at 10, 17, 25, and 40 degrees C in 0.04 M, pH 2 glycine buffer. Alcohol concentrations up to 3.4 M were employed. Four regions in the dependence of the enthalpy of transfer ...

journal_title：Biochemistry

authors： Sturtevant JM,Velicelebi G

更新日期：1981-05-26 00:00:00

abstract：:This paper describes the effect of N-capping substitutions on the structure and stability of histidine-containing protein (HPr). We have used NMR spectroscopy and conformational stability studies to quantify changes in local and global free energy due to mutagenesis at Ser46, the N-cap for helix B in HPr. Previous NMR...

journal_title：Biochemistry

authors： Thapar R,Nicholson EM,Rajagopal P,Waygood EB,Scholtz JM,Klevit RE

更新日期：1996-09-03 00:00:00

abstract：:By exposing photosystem II (PSII) samples to an incrementing number of excitation flashes at room temperature, followed by freezing, we could compare the Mn-derived multiline EPR signal from the S2 oxidation state as prepared by 1, 5, 10, and 25 flashes of light. While the S2 multiline signals exhibited by these sampl...

journal_title：Biochemistry

authors： Peterson S,Ahrling KA,Högblom JE,Styring S

更新日期：2003-03-11 00:00:00

abstract：:Translocation of DNA during in vitro DNA synthesis on nuclear matrix bound replicational assemblies from regenerating rat liver was determined by measuring the processivity (average number of nucleotides added following one productive binding event of the polymerase to the DNA template) of nuclear matrix bound DNA pol...

journal_title：Biochemistry

authors： Tubo RA,Martelli AM,Berezney R

更新日期：1987-09-08 00:00:00

abstract：:The purified ATP-synthesizing complex from chloroplasts has been reconstituted into phospholipid vesicles with bacteriorhodopsin by use of octyl glucoside. Phosphorylation rates up to 90 mmol of ATP (mg of protein)-1 min-1 have been achieved. The dependence of the steady-state kinetic parameters on external and intern...

journal_title：Biochemistry

authors： Takabe T,Hammes GG

更新日期：1981-11-24 00:00:00

abstract：:Calcium trapped within sonicated and resealed bovine rod outer segment disks is released upon light exposure with a stoichiometry of 0.75 +/- 0.05 calcium for each rhodopsin bleached. The amount of calcium liberated is proportional to the amount of bleaching in the range of 20 to 100% bleaching and is relatively insen...

journal_title：Biochemistry

authors： Smith HG Jr,Fager RS,Litman RJ

更新日期：1977-04-05 00:00:00

abstract：:We have developed a simple method for reconstituting pure, integral membrane proteins into phospholipid-protein vesicles. The method does not depend on use of detergents or sonication. It has been used successfully with three different types of integral membrane proteins: UDPglucuronosyltransferase (EC 2.4.1.17) from ...

journal_title：Biochemistry

authors： Scotto AW,Zakim D

更新日期：1985-07-16 00:00:00

abstract：:Alginate is a heteropolysaccharide that consists of β-D-mannuronate (M) and α-L-guluronate (G). The Gram-negative bacterium Sphingomonas sp. A1 directly incorporates alginate into the cytoplasm through the periplasmic solute-binding protein (AlgQ1 and AlgQ2)-dependent ABC transporter (AlgM1-AlgM2/AlgS-AlgS). Two bindi...

journal_title：Biochemistry

authors： Nishitani Y,Maruyama Y,Itoh T,Mikami B,Hashimoto W,Murata K

更新日期：2012-05-01 00:00:00

abstract：:Ankyrin repeat (AR) proteins possess a distinctive modular and repetitive architecture, and their global folds are maintained by short-range interactions in terms of the primary sequence. In this work, we extended our previous study on the highly conserved TPLH tetrapeptide and investigated the impact of a solvent-exp...

journal_title：Biochemistry

authors： Yuan C,Guo Y,Zhu L,Guo W,Mahajan A,Weghorst CM,Li J

更新日期：2013-07-16 00:00:00

abstract：:The respiratory nitric oxide reductase (NOR) from Paracoccus denitrificans catalyzes the two-electron reduction of NO to N(2)O (2NO + 2H(+) + 2e(-) --> N(2)O + H(2)O), which is an obligatory step in the sequential reduction of nitrate to dinitrogen known as denitrification. NOR has four redox-active cofactors, namely,...

journal_title：Biochemistry

authors： Flock U,Watmough NJ,Adelroth P

更新日期：2005-08-09 00:00:00

abstract：:The reactions of fully reduced and mixed-valence bovine heart cytochrome c oxidase with dioxygen have been reinvestigated in the absence and presence of metal ions (Zn(2+), Ni(2+), and Cd(2+)) by time-resolved optical absorption spectroscopy using the CO flow-flash technique. The time-resolved data were recorded on a ...

journal_title：Biochemistry

authors： Szundi I,Cappuccio J,Einarsdóttir O

更新日期：2004-12-21 00:00:00

abstract：:The chirality of sulfoxidation catalyzed by two cytochrome P-450 isozymes purified from phenobarbital-induced rat liver was studied by using 4-tolyl ethyl sulfide as a substrate. Both P-450 isoenzymes, termed PB-1 and PB-4, when reconstituted with purified rat liver NADPH-cytochrome P-450 reductase and cytochrome b5, ...

journal_title：Biochemistry

authors： Waxman DJ,Light DR,Walsh C

更新日期：1982-05-11 00:00:00

abstract：:The interaction of the zinc finger protein WT1 with RNA aptamers has been investigated using a quantitative binding assay, and the results have been compared to those from a previous study of the DNA binding properties of this protein. A recombinant peptide containing the four zinc fingers of WT1 (WT1-ZFP) binds to re...

journal_title：Biochemistry

authors： Zhai G,Iskandar M,Barilla K,Romaniuk PJ

更新日期：2001-02-20 00:00:00

abstract：:The highly conserved amino acid sequence PCDGPGRGGTC in both photosystem I reaction center core proteins PsaA and PsaB has been predicted to contribute the four cysteine ligands for coordination of the 4Fe-4S iron-sulfur cluster FX, and we have proposed a working model for the binding of PsaC to this domain of the rea...

journal_title：Biochemistry

authors： Rodday SM,Webber AN,Bingham SE,Biggins J

更新日期：1995-05-16 00:00:00

abstract：:Spectrin dimers interact weakly with F-actin under physiological solvent conditions (with an association constant of about 5 X 10(3) M-1 at 20 degrees C). In the presence of the membrane skeletal constituent, protein 4.1, strong binding is observed; an analysis of the profiles for formation of a ternary complex leads ...

journal_title：Biochemistry

authors： Ohanian V,Wolfe LC,John KM,Pinder JC,Lux SE,Gratzer WB

更新日期：1984-09-11 00:00:00