Analysis of the ternary interaction of the red cell membrane skeletal proteins spectrin, actin, and 4.1.

abstract：:The heme environmental structures of lactoperoxidase (LP) have been studied by the use of hyperfine-shifted proton NMR and optical absorption spectra. The NMR spectra of the enzyme in native and cyanide forms in H2O indicated that the fifth ligand of the heme iron is the histidyl imidazole with an anionic character an...

journal_title：Biochemistry

authors： Shiro Y,Morishima I

更新日期：1986-10-07 00:00:00

abstract：:Recent studies of DNA hairpin loops have shown considerable dependence of the stability on the sequence of the loop [Senior, M., Jones, R. A., & Breslauer, K. J. (1988a) Proc. Natl. Acad. Sci. U.S.A. 85, 6242-6246; Xodo, L. E., Manzini, G., Quadrifoglio, F., van der Marel, G., & van Boom, J. H. (1989) Biochimie 71, 79...

journal_title：Biochemistry

authors： Wang S,Booher MA,Kool ET

更新日期：1994-04-19 00:00:00

abstract：:Ribonucleotides are frequently incorporated into DNA during replication. They are recognized and processed by several cellular enzymes, and their continued presence in the yeast nuclear genome results in replicative stress and genome instability. Thus, it is important to understand the effects of isolated ribonucleoti...

journal_title：Biochemistry

authors： DeRose EF,Perera L,Murray MS,Kunkel TA,London RE

更新日期：2012-03-27 00:00:00

abstract：:We have studied the pathway and kinetics of refolding of recombinant human brain-derived neurotrophic factor (BDNF), which is a very tightly-associated dimer in its native state. When BDNF unfolded in 6 M guanidine hydrochloride is diluted 20-fold into phosphate-buffered saline, a partially folded intermediate is rapi...

journal_title：Biochemistry

authors： Philo JS,Rosenfeld R,Arakawa T,Wen J,Narhi LO

更新日期：1993-10-12 00:00:00

abstract：:Molecular interactions between the tumor suppressor p53 and the transcriptional coactivators CBP/p300 are critical for the regulation of p53 transactivation and stability. The transactivation domain (TAD) of p53 binds directly to several CBP/p300 domains (TAZ1, TAZ2, NCBD, and KIX). Here we map the interaction between...

journal_title：Biochemistry

authors： Lee CW,Arai M,Martinez-Yamout MA,Dyson HJ,Wright PE

更新日期：2009-03-17 00:00:00

abstract：:D-Xylose isomerase (XI) and triosephosphate isomerase (TIM) catalyze the aldose-ketose isomerization reactions of D-xylose and d-glyceraldehyde 3-phosphate (DGAP), respectively. D-Glyceraldehyde (DGA) is the triose fragment common to the substrates for XI and TIM. The XI-catalyzed isomerization of DGA to give dihydrox...

journal_title：Biochemistry

authors： Toteva MM,Silvaggi NR,Allen KN,Richard JP

更新日期：2011-11-22 00:00:00

abstract：:To determine the active site cysteine residue in aldehyde dehydrogenase, we mutated amino acid residues 49, 162, and 302 of recombinantly expressed rat liver mitochondrial (class 2) aldehyde dehydrogenase. The C49A and C162A mutants were fully active tetrameric enzymes, although the C162A mutant was found to be highly...

journal_title：Biochemistry

authors： Farrés J,Wang TT,Cunningham SJ,Weiner H

更新日期：1995-02-28 00:00:00

abstract：:We report the genetically encoded chemical decaging strategy for protein activation in living bacterial cells. In contrast to the metabolically labile photocaging groups inside Escherichia coli, our chemical decaging strategy that relies on the inverse electron-demand Diels-Alder (iDA) reaction is compatible with the ...

journal_title：Biochemistry

authors： Liu L,Liu Y,Zhang G,Ge Y,Fan X,Lin F,Wang J,Zheng H,Xie X,Zeng X,Chen PR

更新日期：2018-01-30 00:00:00

abstract：:The structural domains of human apolipoprotein(a) [apo(a)] and its interaction with apolipoprotein B-100 (apo B-100) in the lipoprotein(a) [Lp(a)] particle were investigated by limited proteolysis with thermolysin and cathepsin D. We characterized the proteolytic products by sodium dodecyl sulfate-polyacrylamide gradi...

journal_title：Biochemistry

authors： Huby T,Doucet C,Dieplinger H,Chapman J,Thillet J

更新日期：1994-03-22 00:00:00

abstract：:The DNA repair enzyme MutY plays an important role in the prevention of DNA mutations resulting from the presence of the oxidatively damaged lesion 7,8-dihydro-8-oxo-2'-deoxyguanosine (OG) in DNA by the removal of misincorporated adenine residues in OG:A mispairs. MutY also exhibits adenine glycosylase activity toward...

journal_title：Biochemistry

authors： Porello SL,Leyes AE,David SS

更新日期：1998-10-20 00:00:00

abstract：:The X-ray crystal structures of Escherichia coli gamma-aminobutyrate aminotransferase unbound and bound to the inhibitor aminooxyacetate are reported. The enzyme crystallizes from ammonium sulfate solutions in the P3(2)21 space group with a tetramer in the asymmetric unit. Diffraction data were collected to 2.4 A reso...

journal_title：Biochemistry

authors： Liu W,Peterson PE,Carter RJ,Zhou X,Langston JA,Fisher AJ,Toney MD

更新日期：2004-08-31 00:00:00

abstract：:Nitric oxide (NO) regulates cardiovascular hemostasis by binding to soluble guanylyl cyclase (sGC), leading to cGMP production, reduced cytosolic calcium concentration ([Ca(2+)](i)), and vasorelaxation. Thrombospondin-1 (TSP-1), a secreted matricellular protein, was recently discovered to inhibit NO signaling and sGC ...

journal_title：Biochemistry

authors： Ramanathan S,Mazzalupo S,Boitano S,Montfort WR

更新日期：2011-09-13 00:00:00

abstract：:Messenger RNA degradation is an important component of overall gene expression. During the final step of eukaryotic mRNA degradation, exoribonuclease 1 (Xrn1) carries out 5' → 3' processive, hydrolytic degradation of RNA molecules using divalent metal ion catalysis. To initiate studies of the 5' → 3' RNA decay machine...

journal_title：Biochemistry

authors： Langeberg CJ,Welch WRW,McGuire JV,Ashby A,Jackson AD,Chapman EG

更新日期：2020-04-21 00:00:00

abstract：:The transferrins (TFs) are a family of proteins that are widely distributed in vertebrates, where they serve a major role in iron binding and transport. Most TFs are composed of two homologous lobes, the N- and C-lobes, each able to bind a single iron atom. Human serum transferrin (hTF) binds iron in the blood and del...

journal_title：Biochemistry

authors： Halbrooks PJ,He QY,Briggs SK,Everse SJ,Smith VC,MacGillivray RT,Mason AB

更新日期：2003-04-08 00:00:00

abstract：:The Escherichia coli Ada protein repairs methyl phosphotriesters in DNA by direct, irreversible methyl transfer to one of its own cysteine residues. The methyl-transfer process appears to be autocatalyzed by coordination of the acceptor residue, Cys-69, to a tightly bound zinc ion. Upon methyl transfer, Ada acquires t...

journal_title：Biochemistry

authors： Myers LC,Verdine GL,Wagner G

更新日期：1993-12-28 00:00:00

abstract：:Regulation of cellular response is an important mechanism for controlling cellular functions. The transmembrane signaling of the hormone receptors is regulated by GTP-binding proteins (GTPases) and their associated proteins. Our previous studies demonstrated that the bifunctional GTP-binding protein, G alpha h (transg...

journal_title：Biochemistry

authors： Baek KJ,Das T,Gray CD,Desai S,Hwang KC,Gacchui R,Ludwig M,Im MJ

更新日期：1996-02-27 00:00:00

abstract：:It is known that, while melittin at micromolar concentrations is unfolded under conditions of low ionic strength at neutral pH, it adopts a tetrameric alpha-helical structure under conditions of high ionic strength, at alkaline pH, or at high peptide concentrations. To understand the mechanism of the conformational tr...

journal_title：Biochemistry

authors： Goto Y,Hagihara Y

更新日期：1992-01-28 00:00:00

abstract：:DrrA and DrrB proteins form an ATP-dependent efflux pump for doxorubicin and daunorubicin in Streptomyces peucetius. DrrA, the catalytic subunit, forms a complex with the integral membrane protein DrrB. Previous studies have provided evidence for strong interaction between these two proteins, which was found to be cri...

journal_title：Biochemistry

authors： Rao DK,Kaur P

更新日期：2008-03-04 00:00:00

abstract：:Enterokinase has been isolated from the contents of bovine duodena and purified 1200-fold in 41% yield. The isolation procedure employed DEAE chromatography, affinity chromatography on immobilized p-aminobenzamidine, and gel filtration. The resultant pure enzyme exhibits a single band on sodium dodecyl sulfate gel ele...

journal_title：Biochemistry

authors： Anderson LE,Walsh KA,Neurath H

更新日期：1977-07-26 00:00:00

abstract：:Aquaporin-4 (AQP4) is a water channel found at high concentrations around blood vessels in the brain and is organized into elaborate assemblies called square arrays. The natural functions of AQP4 and the square arrays remain unknown, but under pathophysiological conditions, AQP4 has been shown to influence brain edema...

journal_title：Biochemistry

authors： Strand L,Moe SE,Solbu TT,Vaadal M,Holen T

更新日期：2009-06-30 00:00:00

abstract：:The selective reaction of SH containing proteins and peptides with NTCB (2-nitro-5-thiocyanobenzoic acid) has been reported (Degani, Y., & Patchornick, A. (1974) Biochemistry 13, 1; Jacobson, G.A., Schaffer, M.H., Stark, G.R., & Vanaman, T.C. (1973) J. Biol. Chem. 248, 6583). With this reagent, cysteinyl peptide bonds...

journal_title：Biochemistry

authors： Otieno S

更新日期：1978-12-12 00:00:00

abstract：:Kirromycin and related antibiotics inhibit protein synthesis in bacteria by acting on elongation factor Tu (EF-Tu). We have studied the effects of N-methylkirromycin (aurodox) on some molecular properties of this protein. The binding of the antibiotic causes a dramatic variation in the protein fluorescence emission sp...

journal_title：Biochemistry

authors： Balestrieri C,Giovane A,Quagliuolo L,Servillo L,Chinali G

更新日期：1989-08-22 00:00:00

abstract：:The iron-sulfur flavoenzyme adenosine-5'-phosphosulfate (APS) reductase catalyzes a key reaction of the global sulfur cycle by reversibly transforming APS to sulfite and AMP. The structures of the dissimilatory enzyme from Archaeoglobus fulgidus in the reduced state (FAD(red)) and in the sulfite adduct state (FAD-sulf...

journal_title：Biochemistry

authors： Schiffer A,Fritz G,Kroneck PM,Ermler U

更新日期：2006-03-07 00:00:00

abstract：:Cytochrome c-553 from Desulfovibrio vulgaris exhibits a highly exposed heme and an unusually low reduction potential with respect to other c-type cytochromes. Solvent heme exposure has been indicated as one of the most important factors in modulating the midpoint potential of the redox center. To test this hypothesis,...

journal_title：Biochemistry

authors： Fantuzzi A,Sadeghi S,Valetti F,Rossi GL,Gilardi G

更新日期：2002-07-09 00:00:00

abstract：:The doublet at 850 and 830 cm-1 in the Raman spectra of proteins containing tyrosyl residues has been examined as to its origin and the relation of its components to the environment of the phenyl ring, the state of the phenolic hydroxyl group, and the conformation of the amino acid backbone. Raman spectral studies on ...

journal_title：Biochemistry

authors： Siamwiza MN,Lord RC,Chen MC,Takamatsu T,Harada I,Matsuura H,Shimanouchi T

更新日期：1975-11-04 00:00:00

abstract：:The selectivity of binding of Escherichia coli RNA polymerase holoenzyme to a promoter-containing fragment of T7 DNA has been investigated over a range of solution conditions by using a double-label nitrocellulose filter binding assay. A 32P-labeled HaeIII restriction fragment of T7 D111 DNA containing the A1 and D pr...

journal_title：Biochemistry

authors： Strauss HS,Burgess RR,Record MT Jr

更新日期：1980-07-22 00:00:00

abstract：:The equilibrium binding and association kinetics of the Saccharomyces cerevisiae TATA Binding Protein (TBP) to the E4 and Major Late promoters of adenovirus (TATATATA and TATAAAAG, respectively), have been directly compared by quantitative DNase I titration and quench-flow "footprinting". The equilibrium binding of TB...

journal_title：Biochemistry

authors： Petri V,Hsieh M,Jamison E,Brenowitz M

更新日期：1998-11-10 00:00:00

abstract：:Kinetics studies in homogeneous aqueous solution showed that solubilized penicillin-binding protein 2a (sPBP2a) of methicillin-resistant Staphylococcus aureus (a bacterial DD-peptidase) was inhibited by the amphiphilic glycolipid antibiotic moenomycin. Inhibition at the peptidase site was determined by competition exp...

journal_title：Biochemistry

authors： Graves-Woodward K,Pratt RF

更新日期：1999-08-10 00:00:00

abstract：:Apolipophorin III (apoLp-III) from the migratory locust, Locusta migratoria, represents the only full-length apolipoprotein whose three-dimensional structure has been solved. In the present study, spectroscopic methods have been employed to investigate the effects of deglycosylation (via endoglycosidase F treatment) a...

journal_title：Biochemistry

authors： Weers PM,Kay CM,Oikawa K,Wientzek M,Van der Horst DJ,Ryan RO

更新日期：1994-03-29 00:00:00

abstract：:A covalent dimer of Saccharomyces cerevisiae iso-1 cytochrome c is stabilized by an interchain disulfide bond involving the cysteine residue penultimate to the C-terminus. The individual chains in the dimer appear to retain the tertiary structural features characteristic for monomeric cytochrome c albeit with some per...

journal_title：Biochemistry

authors： Bryant C,Strottmann JM,Stellwagen E

更新日期：1985-07-02 00:00:00