Abstract:
:The doublet at 850 and 830 cm-1 in the Raman spectra of proteins containing tyrosyl residues has been examined as to its origin and the relation of its components to the environment of the phenyl ring, the state of the phenolic hydroxyl group, and the conformation of the amino acid backbone. Raman spectral studies on numerous model molecules related to tyrosine, including certain deuterium derivatives, show that the doublet is due to Fermi resonance between the ring-breathing vibration and the overtone of an out-of-plane ring-bending vibration of the para-substituted benzenes. Further examination of the effects of pH and solvents on the Fermi doublet and of the crystallographic data demonstrates that the intensity ratio of the two components depends on changes in the relative frequencies of the two vibrations. These in turn are found to be sensitive to the nature of the hydrogen bonding of the phenolic hydroxyl group of its ionization, but much less so to the environment of the phenyl ring and the conformation of the amino acid backbone. By use of the relative intensities of the doublet in model systems where the phenolic hydroxyl group is strongly hydrogen-bonded, weakly hydrogen-bonded, free or ionized, the reported Raman intensities of the doublets observed in the Raman spectra of several proteins have been interpreted. The results are compared with those obtained by other techniques.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Siamwiza MN,Lord RC,Chen MC,Takamatsu T,Harada I,Matsuura H,Shimanouchi Tdoi
10.1021/bi00693a014subject
Has Abstractpub_date
1975-11-04 00:00:00pages
4870-6issue
22eissn
0006-2960issn
1520-4995journal_volume
14pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::X-ray absorption spectra are reported for the multi-Cu oxidase Rhus vernicifera laccase in oxidized and fully reduced forms and for laccase from which the type 2 Cu has been depleted (T2D). The structure of the Cu K edge for both preparations shows the presence of CuII and CuI in the oxidized and reduced states, respe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00310a008
更新日期:1984-07-17 00:00:00
abstract::The reduced hydroxylase component (MMOH) of soluble methane monooxygenase (MMO) from Methylosinus trichosporium OB3b reacts with O2 and CH4 to produce CH3OH and H2O in a single-turnover reaction. Transient kinetic analysis of this reaction has revealed at least five and probably six intermediates during the turnover [...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi960596w
更新日期:1996-08-06 00:00:00
abstract::We have investigated the microsecond rotational motions of the Ca-ATPase in rabbit skeletal sarcoplasmic reticulum (SR), by measuring the time-resolved phosphorescence anisotropy of erythrosin 5-isothiocyanate (ERITC) covalently and specifically attached to the enzyme. Over a wide range of solvent conditions and tempe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00468a017
更新日期:1990-04-24 00:00:00
abstract::Diabetic cardiomyopathy is the result of maladaptive changes in energy homeostasis. However, the biochemical mechanisms underlying dysfunctional lipid metabolism in diabetic myocardium are incompletely understood. Herein, we exploit shotgun lipidomics to demonstrate a 4-fold increase in acylcarnitines in diabetic myoc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi047773a
更新日期:2005-04-05 00:00:00
abstract::The kcat and Km values for the bovine heart low molecular weight phosphotyrosyl protein phosphatase catalyzed hydrolysis of 16 aryl phosphate monoesters and of five alkyl phosphate monoesters having the structure Ar(CH2)nOPO3H2 (n = 1-5) were measured at pH 5.0 and 37 degrees C. With the exception of alpha-naphthyl ph...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00101a006
更新日期:1991-09-17 00:00:00
abstract::Strong inward-rectifier K(+) (Kir) channels play a significant role in shaping the cardiac action potential: they help produce its long plateau and accelerate its rate of repolarization. Consequently, genetic deletion of the gene encoding the strongly rectifying K(+) channel IRK1 (Kir2.1) prolongs the cardiac action p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0483099
更新日期:2004-12-14 00:00:00
abstract::The Escherichia coli Ada protein repairs methylphosphotriesters in DNA through direct, irreversible transfer to a cysteine residue on the protein, Cys 69. Methylation of Cys 69 increases the sequence-specific DNA-binding activity of Ada by 10(3)-fold, enabling the methylated protein to activate transcription of a meth...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011001m
更新日期:2001-09-25 00:00:00
abstract::Deazapurine-containing secondary metabolites comprise a broad range of structurally diverse nucleoside analogues found throughout biology, including various antibiotics produced by species of Streptomyces bacteria and the hypermodified tRNA bases queuosine and archaeosine. Despite early interest in deazapurines as ant...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900400e
更新日期:2009-05-12 00:00:00
abstract::Heparin was carboxyl-reduced with sodium boro[3H]hydride and converted to a mixture of oligosaccharides by treatment with nitrous acid at pH 2. The oligosaccharide mixture was aldehyde-reduced with sodium boro[3H]hydride and the mixture of products, labeled both in the hexoses formed in the carboxyl-reduction step and...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00663a006
更新日期:1976-09-07 00:00:00
abstract::The interaction of serine protease (esterases) with 6-chloro-2-pyrones was investigated. Time-dependent inactivation of chymotrypsin, alpha-lytic protease, pig liver elastase, and cholinesterase was found with 3- and 5-benzyl-6-chloro-2-pyrone, as well as 3- and 5-methyl-6-chloro-2-pyrone. No inactivation was observed...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00282a034
更新日期:1983-06-21 00:00:00
abstract::Intensive study has been devoted to understanding the kinetic and structural bases underlying the exceptionally high fidelity (low error frequencies) of the typical DNA polymerase. Commonly proposed explanations have included (i) the concept of fidelity check points, in which the correctness of a nascent base pair mat...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi026021i
更新日期:2002-08-27 00:00:00
abstract::Phosphoprotein particles were isolated in their native state from the physiological fluid of the estuarine clam Rangia cuneata , and the characteristics of the mineral ion-protein complex which constitutes the native particle were investigated by using mineral ion binding and mineral ion exchange techniques. The parti...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00302a017
更新日期:1984-03-27 00:00:00
abstract::A reinvestigation of the metal ion activation and specificity of CTP synthetase was begun in order to separate effects due to binding of free metal ions and binding of nucleotide-complexed ions. Apo-CTP synthetase was prepared by dialysis against 5 mM EDTA. Analysis of apo-enzyme by atomic absorption spectroscopy reve...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00065a032
更新日期:1993-04-13 00:00:00
abstract::Rat organic anion transporting protein 1a1 (oatp1a1), a hepatocyte basolateral plasma membrane protein, mediates transport of various amphipathic compounds. Our previous studies indicated that serine phosphorylation of a single tryptic peptide inhibits its transport activity without changing its cell surface content. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi052437v
更新日期:2006-03-14 00:00:00
abstract::The introduction into peptide chains of alpha-aminoisobutyric acid (Aib) has proven to stabilize the helical structure in short peptides by restricting the available range of polypeptide backbone conformations. In order to evaluate the potential stabilizing effect of Aib at the protein level, we have studied the confo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971937o
更新日期:1998-02-10 00:00:00
abstract::The ZFY protein is a member of one of the most interesting classes of polydactyl zinc finger proteins. It has a domain of 13 tandem zinc fingers that is organized with an internal repeat of odd-even finger pairs. It has been proposed that each finger pair interacts with six base pairs within a turn of the double helix...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9018626
更新日期:2010-02-02 00:00:00
abstract::Deletion of Glu146, Thr147, and Trp148 from thrombin dramatically alters its interactions with substrates, ligands, and inhibitors, and the changes appear to result from nonlocal modification of thrombin's structure [Le Bonniec, B. F., Guinto, E. R., & Esmon, C. T. (1992) J. Biol. Chem. 267, 19341-19348]. In an attemp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00179a023
更新日期:1994-04-05 00:00:00
abstract::Oxidized cytochrome c oxidase exists in two reasonably well-defined conformations, a high-spin conformation with maximal absorption at 418 nm and a low-spin conformation with maximal absorption at 428 nm. The equilibrium between these two conformations has been studied as a function of pH, pressure, and temperature. p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00265a009
更新日期:1982-10-26 00:00:00
abstract::The proton-translocating NADH-quinone oxidoreductase (NDH-1) of Paracoccus denitrificans is composed of 14 different subunits (Nqo1-Nqo14). Of these, seven subunits (Nqo7, Nqo8, and Nqo10-14) which are equivalent to the mitochondrial DNA-encoded subunits of complex I constitute the membrane segment of the enzyme compl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034166z
更新日期:2003-04-22 00:00:00
abstract::A heterologous expression system was used to evaluate activation of BlaR1, a sensor/signal transducer protein of Staphylococcus aureus with a central role in resistance to β-lactam antibiotics. In the absence of other S. aureus proteins that might respond to antibiotics and participate in signal transduction events, w...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300429p
更新日期:2012-06-12 00:00:00
abstract::An endogenous calcium-activated sulfhydryl protease in chick oviduct cytosol has been utilized to study the structure of the chick oviduct progesterone receptor subunits, progestophilins A (79 000 g/mol) and B (117 000 g/mol). The protease is not a normal component of the native progesterone receptor aggregate (6 and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00543a015
更新日期:1980-01-22 00:00:00
abstract::Reactivation of tetrameric porcine skeletal muscle lactic dehydrogenase after dissociation and extensive unfolding of the monomers by 6 M guanidine hydrochloride (Gdn . HCl) is characterized by sigmoidal kinetics, indicating a complex mechanism involving rate-limiting folding and association steps. For analysis of the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00260a007
更新日期:1982-08-17 00:00:00
abstract::Terpene synthases are potentially useful biocatalysts for the synthesis of valuable compounds, such as anticancer drugs and antibiotics. The design of altered activities requires better knowledge of their mechanisms, for example, an understanding of the complex conformational changes that are part of their catalytic c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi400898k
更新日期:2013-11-12 00:00:00
abstract::Holliday junctions are central intermediates in the process of genetic recombination. These DNA molecules contain four double helices that flank a central branch point, so that each of the four strands that constitute the junction is associated with two different helices. Previously, we have shown that such branched j...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00003a026
更新日期:1995-01-24 00:00:00
abstract::A 35-amino acid peptide corresponding to the putative "zinc finger" sequence of primase was prepared to study its zinc binding properties. When zinc was added to the peptide, it was found that the fluorescence quantum yield of the single tyrosine increased by 46% and the average lifetime by 34%. The binding stoichiome...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9612778
更新日期:1997-01-21 00:00:00
abstract::5,6-Dihydrothymidine 5'-triphosphate (DHdTTP) was synthesized by catalytic hydrogenation of thymidine 5'-triphosphate (dTTP). Thymidine glycol 5'-triphosphate (dTTP-GLY) was prepared by bromination of dTTP followed by treatment with Ag2O. The modified nucleotides were extensively purified by anion-exchange high-perfor...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00377a042
更新日期:1987-02-10 00:00:00
abstract::Hydrogenase from Chromatium vinosum contains 1 Ni, 11-12 Fe, and ca. 9 sulfides. EPR and Mössbauer studies of the enzyme prepared in four different oxidation states show that the enzyme contains two Fe4S4 and one Fe3S4 cluster. In the oxidized (2+) state, the Mössbauer parameters of the two Fe4S4 clusters are typical ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00182a029
更新日期:1994-04-26 00:00:00
abstract::Membranes derived from free floating granulosa cells in porcine ovarian follicular fluid were used as a starting material for structural characterization of both LH/hCG and FSH receptors. The receptors were highly hormone-specific and showed single classes of high-affinity binding sites (Kd = 19-74 pM). Their molecula...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00467a022
更新日期:1990-04-17 00:00:00
abstract::Mammalian Na+/H+ exchangers (NHEs) play a fundamental role in cellular ion homeostasis. NHEs exhibit an appreciable variation in expression, regulation, and physiological function, dictated by their dynamics in subcellular localization and/or interaction with regulatory proteins. In recent years, a subgroup of NHEs co...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi101082e
更新日期:2011-02-01 00:00:00
abstract::The crystal structures of metyrapone- and 1-, 2-, and 4-phenylimidazole-inhibited complexes of cytochrome P-450cam have been refined to a nominal resolution of 2.1 A and compared with the 1.63-A camphor-bound structure. With the exception of 2-phenylimidazole, each of the inhibitors forms an N-Fe bond with the heme ir...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00399a022
更新日期:1987-12-15 00:00:00