当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Generation of a free alpha-amino group by Raney nickel after 2-nitro-5-thiocyanobenzoic acid cleavage at cysteine residues: application to automated sequencing.

Generation of a free alpha-amino group by Raney nickel after 2-nitro-5-thiocyanobenzoic acid cleavage at cysteine residues: application to automated sequencing.

Abstract：

:The selective reaction of SH containing proteins and peptides with NTCB (2-nitro-5-thiocyanobenzoic acid) has been reported (Degani, Y., & Patchornick, A. (1974) Biochemistry 13, 1; Jacobson, G.A., Schaffer, M.H., Stark, G.R., & Vanaman, T.C. (1973) J. Biol. Chem. 248, 6583). With this reagent, cysteinyl peptide bonds are selectively cyanylated and subsequently cleaved under alkaline conditions. In the present study we have successfully cleaved the beta-chains of guinea pig hemoglobin at the single cysteine and the peptides thus obtained were separated. However, the C-terminal peptide was blocked at its N terminal by a thiazolidine ring and hence could not be used for Edman degradation sequence analysis. Deblocking of this peptide was successfully done by Raney nickel in the buffer medium of pH 7.0, and also in water, at 50 degrees C for 6 to 10 h. The Raney nickel reagent is used in large excess by weight (at least ten times the weight of sulfur compound) over the compound to be desulfurized. Under these conditions, control experiments on cysteine, methionine, and some other amino acids showed that only the sulfur containing amino acids are degraded by Ni(H). Cysteine and methionine were rapidly converted to alanine and beta-aminobutyric acid, respectively. Gel electrophoresis of the iminothiazolidine peptide after exposure to Ni(H) showed no breakage of the chain.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Otieno S

doi

10.1021/bi00618a022

subject

Has Abstract

pub_date

1978-12-12 00:00:00

pages

5468-74

issue

25

eissn

0006-2960

issn

1520-4995

journal_volume

17

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Monoclonal antibodies against distinct determinants of histone H5 bind to chromatin.

    abstract：:A series of monoclonal antibodies specific for distinguishable epitopes in chromosomal protein histone H5 were obtained from mice immunized with either free H5 or H5 . RNA complexes. The antibodies elicited by H5 could be distinguished from those elicited by H5 . RNA by their binding to native or acid-denatured H5, by...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00310a012

    authors： Mendelson E,Bustin M

    更新日期：1984-07-17 00:00:00

  • A synthetic 33-residue analogue of bovine brain calmodulin calcium binding site III: synthesis, purification, and calcium binding.

    abstract：:The sequential solid-phase synthesis of a peptide analogue of bovine brain calmodulin calcium binding site III covering residues 81-113 of the natural sequence is described. Methionine-109 is replaced by a leucine residue to avoid complications in the synthesis and purification. In an attempt to relate the structure o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00393a019

    authors： Reid RE

    更新日期：1987-09-22 00:00:00

  • Novel insights into the INK4-CDK4/6-Rb pathway: counter action of gankyrin against INK4 proteins regulates the CDK4-mediated phosphorylation of Rb.

    abstract：:The newly discovered oncogenic protein gankyrin, which contains six ankyrin repeats, has been reported to be involved in the phosphorylation and degradation of the retinoblastoma gene product, Rb. Using in vitro systems, we have identified a peptide fragment of gankyrin, 176LHLACDEERN185, which is responsible for bind...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi011550s

    authors： Li J,Tsai MD

    更新日期：2002-03-26 00:00:00

  • Mechanistic Insights into R776H Mediated Activation of Epidermal Growth Factor Receptor Kinase.

    abstract：:The epidermal growth factor receptor (EGFR) kinase is activated by a variety of mutations in human cancers. R776H is one such recurrent mutation (R752H in another numbering system) in the αC-β4 loop of the tyrosine kinase domain that activates EGFR in the absence of the activating EGF ligand. However, the mechanistic ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00444

    authors： Ruan Z,Kannan N

    更新日期：2015-07-14 00:00:00

  • Investigation of the regiospecificity and stereospecificity of proton transfer in the yeast inorganic pyrophosphatase catalyzed reaction.

    abstract：:The regiospecificity and stereospecificity of proton transfer in the yeast inorganic pyrophosphatase (PPase) catalyzed hydrolysis of P1,P2-bidentate Mg(H2O)4(PPi)2- were probed with exchange-inert metal complexes of imidodiphosphate (PNP) and thiopyrophosphate (PPS). PPase was unable to catalyze the hydrolysis of Mg(H...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00364a035

    authors： Lin I,Knight WB,Hsueh A,Dunaway-Mariano D

    更新日期：1986-08-12 00:00:00

  • Active site residues of glutamate racemase.

    abstract：:Glutamate racemase, MurI, catalyzes the interconversion of glutamate enantiomers in a cofactor-independent fashion and provides bacteria with a source of D-Glu for use in peptidoglycan biosynthesis. The enzyme uses a "two-base" mechanism involving a deprotonation of the substrate at the alpha-position to form an anion...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi002703z

    authors： Glavas S,Tanner ME

    更新日期：2001-05-29 00:00:00

  • Intersubunit communication in tryptophan synthase by carbon-13 and fluorine-19 REDOR NMR.

    abstract：:The beta subunits of the 143-kDa alpha2beta2 tetrameric enzyme tryptophan synthase have been labeled by L-[ring-4-19F]phenylalanine and L-[phenol-4-13C]tyrosine in an effort to monitor the positions of these residues on ligand binding. Of the 13 phenylalanine and 11 tyrosine residues in the beta subunit, only three pa...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9518297

    authors： McDowell LM,Lee M,McKay RA,Anderson KS,Schaefer J

    更新日期：1996-03-12 00:00:00

  • NSC 622124 inhibits human Eg5 and other kinesins via interaction with the conserved microtubule-binding site.

    abstract：:Kinesin-5 proteins are essential for formation of a bipolar mitotic spindle in most and, perhaps all, eukaryotic cells. Several Kinesin-5 proteins, notably the human version, HsEg5, are targets of a constantly expanding group of small-molecule inhibitors, which hold promise both as tools for probing mechanochemical tr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi801291q

    authors： Learman SS,Kim CD,Stevens NS,Kim S,Wojcik EJ,Walker RA

    更新日期：2009-03-03 00:00:00

  • Role of the tertiary nucleotides in the interaction of yeast phenylalanine tRNA with its cognate synthetase.

    abstract：:In vitro transcription by T7 RNA polymerase was used to prepare 32 different mutations in the 21 nucleotides that participate in the 9 tertiary base pairs or triples of yeast tRNAPhe. The mutations were designed either to disrupt the tertiary interaction or to change the sequence without disrupting the structure by tr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00462a014

    authors： Sampson JR,DiRenzo AB,Behlen LS,Uhlenbeck OC

    更新日期：1990-03-13 00:00:00

  • Incorporation of cationic chains in the Dickerson-Drew dodecamer: correlation of energetics, structure, and ion and water binding.

    abstract：:We have investigated the unfolding thermodynamics for incorporating cationic side chains in the Dickerson-Drew dodecamer duplex. Incorporation of two 3-aminopropyl-2'-deoxyuridine residues (one on each self-complementary strand) lowers the stability of the duplex. This reduction is driven by unfavorable heat contribut...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi050897i

    authors： Shikiya R,Li JS,Gold B,Marky LA

    更新日期：2005-09-20 00:00:00

  • Structure-function relationships of scorpion neurotoxins.

    abstract：:Chemical modification of some trifunctional amino acid residues in toxins I, II, and III of the scorpion Androctonus australis Hector have been performed. The results indicate: (1) Reduction and methylation of one disulfide bridge destroy toxic activity of toxin II. (2) The only tryptophan residue of toxin II (positio...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00656a002

    authors： Habersetzer-Rochat C,Sampieri F

    更新日期：1976-06-01 00:00:00

  • Multiple conformations of NAD and NADH when bound to human cytosolic and mitochondrial aldehyde dehydrogenase.

    abstract：:Crystallographic analysis revealed that the nicotinamide ring of NAD can bind with multiconformations to aldehyde dehydrogenase (ALDH) (Ni, L., Zhou, J., Hurley, T. D., and Weiner, H. (1999) Protein Sci. 8, 2784-2790). Electron densities can be defined for two conformations, neither of which appears to be compatible w...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi012197t

    authors： Hammen PK,Allali-Hassani A,Hallenga K,Hurley TD,Weiner H

    更新日期：2002-06-04 00:00:00

  • Catalytic mechanism of enterococcal kanamycin kinase (APH(3')-IIIa): viscosity, thio, and solvent isotope effects support a Theorell-Chance mechanism.

    abstract：:Bacterial resistance to the aminoglycoside antibiotics is manifested primarily through the production of enzymes which covalently modify these drugs. The Enterococci and Staphylococci produce an ATP-dependent kinase, APH(3')-IIIa, which phosphorylates such antibiotics as kanamycin, amikacin, and neomycin, and this enz...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9603884

    authors： McKay GA,Wright GD

    更新日期：1996-07-02 00:00:00

  • Evidence for a slow tertiary relaxation in the reaction of tert-butyl isocyanide with horseradish peroxidase.

    abstract：:The kinetics of tert-butyl isocyanide binding to the heme protein horseradish peroxidase (HRP) at 22 degrees C was examined on all time scales, from minutes to picoseconds, in aqueous borate buffer at pH 9.08. Unlike myoglobin (Mb) or hemoglobin, HRP shows two bimolecular ligand binding processes. For comparison, bind...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9518149

    authors： Bandyopadhyay D,Walda KN,Grogan TM,Magde D,Traylor TG,Sharma VS

    更新日期：1996-02-06 00:00:00

  • The 56-59-kilodalton protein identified in untransformed steroid receptor complexes is a unique protein that exists in cytosol in a complex with both the 70- and 90-kilodalton heat shock proteins.

    abstract：:It has previously been shown that 9S, untransformed progestin, estrogen, androgen, and glucocorticoid receptor complexes in rabbit uterine and liver cytosols contain a 59-kDa protein [Tai, P. K., Maeda, Y., Nakao, K., Wakim, N. G., Duhring, J. L., & Faber, L. E. (1986) Biochemistry 25, 5269-5275]. In this work we show...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00473a021

    authors： Sanchez ER,Faber LE,Henzel WJ,Pratt WB

    更新日期：1990-05-29 00:00:00

  • A common catalytic mechanism for proteins of the HutI family.

    abstract：:Imidazolonepropionase (HutI) (imidazolone-5-propanote hydrolase, EC 3.5.2.7) is a member of the amidohydrolase superfamily and catalyzes the conversion of imidazolone-5-propanoate to N-formimino-L-glutamate in the histidine degradation pathway. We have determined the three-dimensional crystal structures of HutI from A...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi800180g

    authors： Tyagi R,Eswaramoorthy S,Burley SK,Raushel FM,Swaminathan S

    更新日期：2008-05-20 00:00:00

  • Crystal structure of the acid-induced arginine decarboxylase from Escherichia coli: reversible decamer assembly controls enzyme activity.

    abstract：:The acid-induced arginine decarboxylase is part of an enzymatic system in Escherichia coli that contributes to making this organism acid resistant. The arginine decarboxylase is a vitamin B(6)-dependent enzyme that is active at acidic pH. It consumes a proton in the decarboxylation of arginine to agmatine, and by work...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi900075d

    authors： Andréll J,Hicks MG,Palmer T,Carpenter EP,Iwata S,Maher MJ

    更新日期：2009-05-12 00:00:00

  • Effects of hydration on purine motion in solid DNA.

    abstract：:Deuterium quadrupole echo spectra and spin-lattice relaxation rates measured at 76.8 and 38.4 MHz as a function of relative humidity are reported for calf thymus DNA deuterated at positions A8 and G8. The amplitude of base pair motion is observed to increase slightly with increasing degree of hydration (up to approxim...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00371a069

    authors： Brandes R,Vold RR,Vold RL,Kearns DR

    更新日期：1986-11-18 00:00:00

  • Proton transfer in carbonic anhydrase is controlled by electrostatics rather than the orientation of the acceptor.

    abstract：:Combined quantum mechanical/molecular mechanical (QM/MM) simulations are carried out to analyze factors that dictate the proton transfer in carbonic anhydrase II (CAII), an enzyme that has been used as a prototypical example of long-range proton transfers in biomolecules. In contrast to the long-held conjecture in the...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi701950j

    authors： Riccardi D,König P,Guo H,Cui Q

    更新日期：2008-02-26 00:00:00

  • The acidic phosphoproteins from Saccharomyces cerevisiae ribosomes. NH2-terminal acetylation is a conserved difference between P1 and P2 proteins.

    abstract：:Isoelectrofocusing gels of acidic ribosomal proteins from most yeast strains reveal the presence of up to 10 bands which are the product of only 4 genes. The proteins have been characterized by NH2-terminal amino acid sequencing, specific antibodies, HPLC, and by taking advantage of acidic protein-defective yeast stra...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00067a010

    authors： Santos C,Ortiz-Reyes B,Naranda T,Remacha M,Ballesta JP

    更新日期：1993-04-27 00:00:00

  • Intersubunit association induces unique allosteric dependence of the T127L CRP mutant on pH.

    abstract：:The allosteric activation of the T127-->L mutant of 3',5'-cyclic adenosine monophosphate (cAMP) receptor protein (CRP) by cAMP changes from an exothermic, independent two-site binding mechanism at pH 7.0 to an endothermic, interacting two-site binding mechanism at pH 5.2, similar to that observed for CRP at pH 7.0 and...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi000225m

    authors： Shi Y,Wang S,Schwarz FP

    更新日期：2000-06-20 00:00:00

  • Biotin synthase is a pyridoxal phosphate-dependent cysteine desulfurase.

    abstract：:Biotin synthase (BioB) is an iron-sulfur dimeric enzyme which catalyzes the last step in biotin synthesis. The reaction consists of the introduction of a sulfur atom into dethiobiotin. It is shown here that BioB displays a significant cysteine desulfurase activity, providing it with the ability to mobilize sulfur from...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0122011

    authors： Ollagnier-De-Choudens S,Mulliez E,Hewitson KS,Fontecave M

    更新日期：2002-07-23 00:00:00

  • Organellar Na+/H+ exchangers: novel players in organelle pH regulation and their emerging functions.

    abstract：:Mammalian Na+/H+ exchangers (NHEs) play a fundamental role in cellular ion homeostasis. NHEs exhibit an appreciable variation in expression, regulation, and physiological function, dictated by their dynamics in subcellular localization and/or interaction with regulatory proteins. In recent years, a subgroup of NHEs co...

    journal_title：Biochemistry

    pub_type： 杂志文章,评审

    doi：10.1021/bi101082e

    authors： Ohgaki R,van IJzendoorn SC,Matsushita M,Hoekstra D,Kanazawa H

    更新日期：2011-02-01 00:00:00

  • Structural Basis for Excision of 5-Formylcytosine by Thymine DNA Glycosylase.

    abstract：:Thymine DNA glycosylase (TDG) is a base excision repair enzyme with key functions in epigenetic regulation. Performing a critical step in a pathway for active DNA demethylation, TDG removes 5-formylcytosine and 5-carboxylcytosine, oxidized derivatives of 5-methylcytosine that are generated by TET (ten-eleven transloca...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.6b00982

    authors： Pidugu LS,Flowers JW,Coey CT,Pozharski E,Greenberg MM,Drohat AC

    更新日期：2016-11-15 00:00:00

  • Evolution of enzymatic activities in the enolase superfamily: partitioning of reactive intermediates by (D)-glucarate dehydratase from Pseudomonas putida.

    abstract：:Glucarate dehydratase (GlucD) from Pseudomonas putida catalyzes the dehydration of both (D)-glucarate and (L)-idarate to 3-deoxy-(L)-threo-2-hexulosarate as well as their epimerization. (D)-[6-13C]Glucarate and (L)-[6-13C]idarate have been synthesized for use in continuous assay of the reactions catalyzed by GlucD by ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi981122v

    authors： Palmer DR,Hubbard BK,Gerlt JA

    更新日期：1998-10-13 00:00:00

  • Identification of the C=O stretching vibrations of FMN and peptide backbone by 13C-labeling of the LOV2 domain of Adiantum phytochrome3.

    abstract：:Phototropin, a blue-light photoreceptor in plants, has two FMN-binding domains named LOV1 and LOV2. We previously observed temperature-dependent FTIR spectral changes in the C=O stretching region (amide-I vibrational region of the peptide backbone) for the LOV2 domain of Adiantum phytochrome3 (phy3-LOV2), suggesting p...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi061837v

    authors： Iwata T,Nozaki D,Sato Y,Sato K,Nishina Y,Shiga K,Tokutomi S,Kandori H

    更新日期：2006-12-26 00:00:00

  • PsbU provides a stable architecture for the oxygen-evolving system in cyanobacterial photosystem II.

    abstract：:PsbU is a lumenal peripheral protein in the photosystem II (PS II) complex of cyanobacteria and red algae. It is thought that PsbU is replaced functionally by PsbP or PsbQ in plant chloroplasts. After the discovery of PsbP and PsbQ homologues in cyanobacterial PS II [Thornton et al. (2004) Plant Cell 16, 2164-2175], w...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi047539k

    authors： Inoue-Kashino N,Kashino Y,Satoh K,Terashima I,Pakrasi HB

    更新日期：2005-09-13 00:00:00

  • Geometry of the phosphodiester backbone in the A form of deoxyribonucleic acid determined by phosphorus-31 nuclear magnetic resonance spectroscopy.

    abstract：:31P NMR spectra were obtained from poly(dA-dT) . poly(dA-dT) fibers which gave an X-ray diffraction pattern similar to that of the A form of natural deoxyribonucleic acid (DNA). The analysis of the line shape indicated that the A form of poly(dA-dT) . poly(dA-dT) has a single uniform backbone conformation; the orienta...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00507a013

    authors： Shindo H,Wooten JB,Zimmerman SB

    更新日期：1981-02-17 00:00:00

  • Structural basis for the quinone reduction in the bc1 complex: a comparative analysis of crystal structures of mitochondrial cytochrome bc1 with bound substrate and inhibitors at the Qi site.

    abstract：:Cytochrome bc(1) is an integral membrane protein complex essential to cellular respiration and photosynthesis. The Q cycle reaction mechanism of bc(1) postulates a separated quinone reduction (Q(i)) and quinol oxidation (Q(o)) site. In a complete catalytic cycle, a quinone molecule at the Q(i) site receives two electr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0341814

    authors： Gao X,Wen X,Esser L,Quinn B,Yu L,Yu CA,Xia D

    更新日期：2003-08-05 00:00:00

  • Production and characterization of a functional putidaredoxin reductase-putidaredoxin covalent complex.

    abstract：:In the cytochrome P450cam-dependent monooxygenase system from Pseudomonas putida, putidaredoxin (Pdx) shuttles electrons between putidaredoxin reductase (Pdr) and P450cam and, thus, must form transient complexes with both partners. 1-Ethyl 3-[3-(dimethylamino)propyl]carbodiimide (EDC) was found to promote formation of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi901872s

    authors： Churbanova IY,Poulos TL,Sevrioukova IF

    更新日期：2010-01-12 00:00:00