Structural characterization of lactoperoxidase in the heme environment by proton NMR spectroscopy.

abstract：:Flavocytochrome c-sulfide dehydrogenases (FCSDs) are complexes of a flavoprotein with a c-type cytochrome performing hydrogen sulfide-dependent cytochrome c reduction in vitro. The amino acid sequence analysis revealed that the phylogenetic relationship of different flavoproteins reflected the relationship of sulfur-o...

journal_title：Biochemistry

authors： Quentmeier A,Hellwig P,Bardischewsky F,Wichmann R,Friedrich CG

更新日期：2004-11-23 00:00:00

abstract：:We have used nucleoside phosphorothioates (NTP alpha S) and a substitution-interference method to identify phosphate oxygens that appear to be important to guanosine cofactor addition in the self-splicing group I intron from Tetrahymena thermophila. For the majority of these phosphate oxygens, however, the effect of N...

journal_title：Biochemistry

authors： Christian EL,Yarus M

更新日期：1993-05-04 00:00:00

abstract：:In prokaryotes, the first three enzymes in pyrimidine biosynthesis, carbamoyl phosphate synthetase (CPS), aspartate transcarbamoylase (ATC), and dihydroorotase (DHO), are commonly expressed separately and either function independently (Escherichia coli) or associate into multifunctional complexes (Aquifex aeolicus). I...

journal_title：Biochemistry

authors： Zhang P,Martin PD,Purcarea C,Vaishnav A,Brunzelle JS,Fernando R,Guy-Evans HI,Evans DR,Edwards BF

更新日期：2009-02-03 00:00:00

abstract：:Factor VIII (fVIII) is synthesized as a single chain having a domainal sequence A1-A2-B-A3-C1-C2. Analysis of the proteolyic cleavage of fVIII by thrombin by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) identifies three fragments designated fVIIIA1, fVIIIA2, and fVIIIA3-C1-C2 with fragment(s) d...

journal_title：Biochemistry

authors： Lollar P,Parker CG

更新日期：1989-01-24 00:00:00

abstract：:The 18 kDa translocator protein (TSPO), formerly known as the peripheral-type benzodiazepine receptor, has been reported to be closely associated with the mitochondrial permeability transition pore (MPTP). TSPO is believed to exert pro-apoptotic functions via modulation of MPTP opening. Cobalt chloride (CoCl(2)), whic...

journal_title：Biochemistry

authors： Zeno S,Zaaroor M,Leschiner S,Veenman L,Gavish M

更新日期：2009-06-02 00:00:00

abstract：:The secretory component (SC) isolated from human milk was labeled with 2 mol of the fluorescent thiol reagent N-[7-(dimethylamino)-4-methylcoumarinyl]maleimide (DACM) per mol of SC through the reactive disulfide bond of SC. The binding of the labeled SC to polymeric immunoglobulins was examined by gel filtration by me...

journal_title：Biochemistry

authors： Goto Y,Aki K

更新日期：1984-12-18 00:00:00

abstract：:Nhp6A is an abundant non-histone chromatin-associated protein in Saccharomyces cerevisiae that contains a minor groove DNA binding motif called the HMG box. In this report, we show that Nhp6Ap binds to cisplatin intrastrand cross-links on duplex DNA with a 40-fold greater affinity than to unmodified DNA with the same ...

journal_title：Biochemistry

authors： Wong B,Masse JE,Yen YM,Giannikopoulos P,Feigon J,Johnson RC

更新日期：2002-04-30 00:00:00

abstract：:Native ribonucleotide reductase from Escherichia coli exhibits a resonance-enhanced Raman mode at 1498 cm-1 that is characteristic of a tyrosyl radical. The Raman frequency as well as the absorption maximum at 410 nm identifies the radical as being in a deprotonated state. The B2 subunit of ribonucleotide reductase sh...

journal_title：Biochemistry

authors： Backes G,Sahlin M,Sjöberg BM,Loehr TM,Sanders-Loehr J

更新日期：1989-02-21 00:00:00

abstract：:In the HLH-LZ protein family, the helix-loop-helix DNA-binding dimerization domain is followed in the sequence by a leucine zipper motif. The precise function of this second dimerization domain is still unclear, since the HLH motif of a subset of this family has been shown to be necessary and sufficient for dimerizati...

journal_title：Biochemistry

authors： Muhle-Goll C,Gibson T,Schuck P,Schubert D,Nalis D,Nilges M,Pastore A

更新日期：1994-09-20 00:00:00

abstract：:The pK(a) values measured previously for the internal Lys-66, Asp-66, and Glu-66 in variants of a highly stable form of staphylococcal nuclease are shifted by as many as 5 pK(a) units relative to normal pK(a) values in water. These shifts cannot be reproduced with continuum electrostatics calculations with static stru...

journal_title：Biochemistry

authors： Karp DA,Stahley MR,García-Moreno B

更新日期：2010-05-18 00:00:00

abstract：:The denaturation of the heat shock protein DnaK induced by guanidine hydrochloride (Gdn-HCl) was investigated by circular dichroism, fluorescence, size-exclusion HPLC, and dynamic light scattering. DnaK unfolding takes place in two discrete steps. The midpoint (Cm) of the first transition (0.5 M) was shifted to higher...

journal_title：Biochemistry

authors： Palleros DR,Shi L,Reid KL,Fink AL

更新日期：1993-04-27 00:00:00

abstract：:Catalysis in protein tyrosine phosphatases (PTPs) involves movement of a protein loop called the WPD loop that brings a conserved aspartic acid into the active site to function as a general acid. Mutation of the tryptophan in the WPD loop of the PTP YopH to any other residue with a planar, aromatic side chain (phenyla...

journal_title：Biochemistry

authors： Moise G,Gallup NM,Alexandrova AN,Hengge AC,Johnson SJ

更新日期：2015-10-27 00:00:00

abstract：:Treatment with cholesterol oxidases has shown that cholesterol is heterogeneously distributed in brush border membranes isolated from the apical domain of the renal and intestinal epithelial cells [Bloj, B., & Zilversmit, D. B. (1982) J. Biol. Chem. 257, 7608-7614; El Yandouzi, E. H., & Le Grimellec, C. (1992) Biochem...

journal_title：Biochemistry

authors： el Yandouzi EH,Zlatkine P,Moll G,Le Grimellec C

更新日期：1994-03-01 00:00:00

abstract：:Oligothymidylates covalently linked to iron-porphyrins were synthesized to target the nuclease activity of Fe-porphyrin to complementary polynucleotides. In the presence of oxygen and a reducing agent, oligo(dT)7 bearing the reactive group attached to the 3'-phosphate was shown to be active in the cleavage of poly(dA)...

journal_title：Biochemistry

authors： Le Doan T,Perrouault L,Helene C,Chassignol M,Thuong NT

更新日期：1986-11-04 00:00:00

abstract：:Resonance Raman vibrational spectra of the Pr, lumi-R, and Pfr forms of phytochrome have been obtained using low-temperature trapping and room temperature flow techniques in conjunction with shifted-excitation Raman difference spectroscopy (SERDS). The Pr to lumi-R photoconversion exhibits a thermal barrier and is com...

journal_title：Biochemistry

authors： Andel F 3rd,Lagarias JC,Mathies RA

更新日期：1996-12-17 00:00:00

abstract：:Deoxycytidine kinase (dCK) uses either ATP or UTP as a phosphoryl donor to catalyze the phosphorylation of nucleoside acceptors. The kinetic properties of human dCK are modulated in vivo by phosphorylation of serine 74. This residue is a part of the insert region and is distant from the active site. Replacing the seri...

journal_title：Biochemistry

authors： Hazra S,Szewczak A,Ort S,Konrad M,Lavie A

更新日期：2011-04-12 00:00:00

abstract：:X-ray crystallographic studies have shown that DNA binding by human polymerase beta (pol beta) occurs primarily through two structurally and sequentially homologous helix-hairpin-helix (HhH) motifs, one in the fingers subdomain and the other in the 8-kDa domain [Pelletier, H., Sawaya, M. R., Wolfle, W., Wilson, S. H.,...

journal_title：Biochemistry

authors： Pelletier H,Sawaya MR

更新日期：1996-10-01 00:00:00

abstract：:In eukaryotes, choline acetyltransferase (ChAT) catalyzes the reversible formation of the neurotransmitter acetylcholine from choline and acetyl-CoA. ChAT belongs to a family of CoA-dependent enzymes that also includes the carnitine acyltransferases CrAT, CrOT, and CPTs. In contrast to CrOT and CPTs that are very acti...

journal_title：Biochemistry

authors： Green KD,Porter VR,Zhang Y,Garneau-Tsodikova S

更新日期：2010-07-27 00:00:00

abstract：:Oligomerization of numerous G protein-coupled receptors has been documented, including the prototypic family B secretin receptor. The clinical significance of oligomerization of this receptor became clear with the recent observation that a misspliced form present in pancreatic cancer could associate with the wild-type...

journal_title：Biochemistry

authors： Lisenbee CS,Miller LJ

更新日期：2006-07-11 00:00:00

abstract：:The complete amino acid sequence of human liver cytosolic alanine aminotransferase (GPT) (EC 2.6.1.2) is presented. Two primary sets of overlapping fragments were obtained by cleavage of the pyridylethylated protein at methionyl and lysyl bonds with cyanogen bromide and Achromobacter protease I, respectively. Isolated...

journal_title：Biochemistry

authors： Ishiguro M,Takio K,Suzuki M,Oyama R,Matsuzawa T,Titani K

更新日期：1991-10-29 00:00:00

abstract：:The neutron structure of subtilisin BPN' has been refined and analyzed at 2.0-A resolution. The structure studied was a mutant variant of subtilisin, Met222----Gln, and was used because large, uninhibited crystals could be grown, which was not the case for the native molecule. Comparison of the structure with that of ...

journal_title：Biochemistry

authors： Kossiakoff AA,Ultsch M,White S,Eigenbrot C

更新日期：1991-02-05 00:00:00

abstract：:The envelope glycoprotein of HIV-1 consists of the surface subunit gp120 and the transmembrane subunit gp41. Binding of gp120 to target cell receptors induces a conformational change in gp41, which then mediates the fusion of viral and cellular membranes. A buried isoleucine (Ile573) in a central trimeric coiled coil ...

journal_title：Biochemistry

authors： Liu J,Shu W,Fagan MB,Nunberg JH,Lu M

更新日期：2001-03-06 00:00:00

abstract：:Computer graphics and energy minimization techniques were used to construct a model of DNA containing cis-thymine glycol, an oxidation product of thymine formed in DNA by ionizing radiation. The model simulated an experimental DNA substrate used to study the effects of this lesion on DNA synthesis in vitro. The result...

journal_title：Biochemistry

authors： Clark JM,Pattabiraman N,Jarvis W,Beardsley GP

更新日期：1987-08-25 00:00:00

abstract：:Rh(phen)2phi3+ (phen = 1,10-phenanthroline; phi = 9,10-phenanthrenequinone diimine) avidly binds to DNA via intercalation from the major groove and upon photoactivation produces strand scission with single base 5' asymmetry. Enantiomers of Rh(phen)2phi3+, which lack hydrogen-bonding substituents in ancillary positions...

journal_title：Biochemistry

authors： Campisi D,Morii T,Barton JK

更新日期：1994-04-12 00:00:00

abstract：:Glutaraldehyde treatment of electroplax membrane preparations of Na,K-ATPase leads to irreversible changes in the enzymic behavior of the protein, which are not due to modification of the active site. When the glutaraldehyde treatment is carried out in a medium containing K+ and without Na+, the "K+-modified enzyme" s...

journal_title：Biochemistry

authors： Chipman DM,Lev A

更新日期：1983-09-13 00:00:00

abstract：:The amino acids (residues 39-51) responsible for the interaction between the first intracellular loop (iLP1) of the human prostacyclin receptor (IP) and G alpha s protein have been identified [Zhang, L., Huang, G., Wu, J., and Ruan, K. H. (2005) Biochemistry 44, 11389-11401]. To further characterize the structural/fun...

journal_title：Biochemistry

authors： Zhang L,Wu J,Ruan KH

更新日期：2006-02-14 00:00:00

abstract：:Kinases play a critical role in cellular signaling and are dysregulated in a number of diseases, such as cancer, diabetes, and neurodegeneration. Therapeutics targeting kinases currently account for roughly 50% of cancer drug discovery efforts. The ability to explore human kinase biochemistry and biophysics in the lab...

journal_title：Biochemistry

authors： Albanese SK,Parton DL,Işık M,Rodríguez-Laureano L,Hanson SM,Behr JM,Gradia S,Jeans C,Levinson NM,Seeliger MA,Chodera JD

更新日期：2018-08-07 00:00:00

abstract：:The biosynthesis of osteocalcin (OC), a bone-specific, noncollagenous protein, is stringently regulated during differentiation of the osteoblast phenotype. Glucocorticoids, and also 1,25(OH)2D3, mediate the developmental regulation of OC gene transcription. In this study, we established that the -1097 to +23 promoter ...

journal_title：Biochemistry

authors： Heinrichs AA,Bortell R,Rahman S,Stein JL,Alnemri ES,Litwack G,Lian JB,Stein GS

更新日期：1993-10-26 00:00:00

abstract：:Dendritic cell-specific intracellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN) is a C-type lectin highly expressed on the surface of antigen-presenting dendritic cells. DC-SIGN mediates interactions among dendritic cells, pathogens, and a variety of epithelia, myeloid cells, and endothelia by binding to high...

journal_title：Biochemistry

authors： Pederson K,Mitchell DA,Prestegard JH

更新日期：2014-09-09 00:00:00

abstract：:We have used DNase I footprinting to examine the formation of antiparallel DNA triple helices on DNA fragments containing the homopurine target sites (GGA)2GGX(GGA)2GG.(CCT)2CCZ(CCT)2CC (where X.Z is each base pair in turn), with the GA- and GT-rich oligonucleotides, (GGA)2GGN(GGA)2GG and (GGT)2GGN(GGT)2GG (N = each b...

journal_title：Biochemistry

authors： Chandler SP,Fox KR

更新日期：1996-11-26 00:00:00