Abstract:
:Computer graphics and energy minimization techniques were used to construct a model of DNA containing cis-thymine glycol, an oxidation product of thymine formed in DNA by ionizing radiation. The model simulated an experimental DNA substrate used to study the effects of this lesion on DNA synthesis in vitro. The results derived from the model indicate that cis-thymine glycol lesions introduce localized perturbations of DNA structure. Specifically the model shows that interactions with the neighboring base pair on the 5' side are significantly destabilized by thymine glycol whereas interactions with the 3' base pair are stabilized by the lesion. The magnitude of these effects is modulated by the nucleotide sequence around the lesion, particularly by the nature of the base on the 3' side. The base pair formed between adenine and thymine glycol is energetically stable and shows minimal distortion, suggesting that this lesion retains the ability to direct the insertion of the correct nucleotide during DNA synthesis.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Clark JM,Pattabiraman N,Jarvis W,Beardsley GPdoi
10.1021/bi00391a028subject
Has Abstractpub_date
1987-08-25 00:00:00pages
5404-9issue
17eissn
0006-2960issn
1520-4995journal_volume
26pub_type
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