Pre-steady-state studies of phosphite dehydrogenase demonstrate that hydride transfer is fully rate limiting.

abstract：:PLK1 (polo-like kinase 1) is a key mitotic kinase and a therapeutic target in the treatment of proliferative diseases. Here we investigate the relative substrate specificity and pharmacological relatedness of PLK1, -2, -3, and -4 that together comprise a conserved family of Ser/Thr kinases (PLK family). We report cons...

journal_title：Biochemistry

authors： Johnson EF,Stewart KD,Woods KW,Giranda VL,Luo Y

更新日期：2007-08-21 00:00:00

abstract：:Adriamycin plays a prominent role in the treatment of leukemia and solid tumors in man. The mode of interaction of adriamycin with its nuclear target, responsible for its therapeutic effect, is known [Berman, H. M., & Young, P.R. (1981) Annu. Rev. Biophys. Bioeng. 10, 87-114]. The planar anthracycline moiety of adriam...

journal_title：Biochemistry

authors： Goormaghtigh E,Brasseur R,Huart P,Ruysschaert JM

更新日期：1987-03-24 00:00:00

abstract：:Anaerobic induction of light harvesting and reaction center gene expression involves two transacting factors termed RegA and RegB. Sequence and mutational analysis has indicated that RegA and RegB constitute cognate components of a prokaryotic sensory transduction cascade with RegB comprising a membrane-spanning senso...

journal_title：Biochemistry

authors： Inoue K,Kouadio JL,Mosley CS,Bauer CE

更新日期：1995-01-17 00:00:00

abstract：:Protein splicing is a post-translational reaction facilitated by an intein, or intervening protein, which involves the removal of the intein and the ligation of the flanking polypeptides, or exteins. A DNA polymerase II intein from Pyrococcus abyssi (Pab PolII intein) can promote protein splicing in vitro on incubatio...

journal_title：Biochemistry

authors： Minteer CJ,Siegart NM,Colelli KM,Liu X,Linhardt RJ,Wang C,Gomez AV,Reitter JN,Mills KV

更新日期：2017-02-28 00:00:00

abstract：:Single-molecule fluorescence resonance energy transfer (smFRET) methods were used to study the assembly pathway and DNA unwinding activity of the bacteriophage T4 helicase-primase (primosome) complex. The helicase substrates used were surface-immobilized model DNA replication forks "internally" labeled in the duplex r...

journal_title：Biochemistry

authors： Lee W,Jose D,Phelps C,Marcus AH,von Hippel PH

更新日期：2013-05-07 00:00:00

abstract：:By limited proteolysis of a type kappa immunoglobulin light chain (Oku) with clostripain, both the VL and CL fragments were obtained with a high yield. The unfolding and refolding by guanidine hydrochloride of light chain Oku and its VL and CL fragments were studied at pH 7.5 and 25 degrees C with circular dichroism a...

journal_title：Biochemistry

authors： Tsunenaga M,Goto Y,Kawata Y,Hamaguchi K

更新日期：1987-09-22 00:00:00

abstract：:The involvement of Cl(-) and several other monovalent anions in photosynthetic oxygen evolution was studied using photosystem II membranes depleted of Cl(-) by dialysis. The results of these studies differ significantly from results obtained using other depletion methods. Binding studies with glycerol as a cryoprotect...

journal_title：Biochemistry

authors： Olesen K,Andréasson LE

更新日期：2003-02-25 00:00:00

abstract：:The human ferritin L-chain cDNA was cloned into a vector for overproduction in Escherichia coli, under the regulation of a lambda promoter. The plasmid obtained contains the full L-chain coding region modified at the first two codons. It is able to direct the synthesis of the L-chain which can constitute up to 15% of ...

journal_title：Biochemistry

authors： Levi S,Salfeld J,Franceschinelli F,Cozzi A,Dorner MH,Arosio P

更新日期：1989-06-13 00:00:00

abstract：:Two glutathione S-transferase (GST) activities have been identified and purified from etiolated corn tissue. The first, designated GST I enzyme, is constitutively present in corn tissue, and the second, designated GST II enzyme, is present only in tissue which has been treated with chemical antidotes which protect cor...

journal_title：Biochemistry

authors： Mozer TJ,Tiemeier DC,Jaworski EG

更新日期：1983-03-01 00:00:00

abstract：:Two transcription factors, CRP and CytR, mediate positive and negative control of nine cistrons involved in nucleoside catabolism and recycling in Escherichia coli. The ability of multiple transcription factors to combine in different ways to confer differential gene regulation is of significant interest in both proka...

journal_title：Biochemistry

authors： Holt AK,Senear DF

更新日期：2010-01-26 00:00:00

abstract：:The selective paramagnetic relaxation of oligonucleotide protons of d(GTGCAC)2 by delta- and lambda-Ni(phen)3(3+) and delta- and lambda-Cr(phen)3(3+) has been examined to obtain some structural insight into the noncovalent binding of tris(phenanthroline) metal complexes to DNA. The experiments demonstrate that the rel...

journal_title：Biochemistry

authors： Rehmann JP,Barton JK

更新日期：1990-02-20 00:00:00

abstract：:In this study, we have investigated the influence of regions outside the DNA-binding domain of the human glucocorticoid receptor on high-affinity DNA binding. We find that the DNA-binding domain shows a 10-fold lower affinity for a palindromic DNA-binding site than the intact receptor. The N-terminal part of the recep...

journal_title：Biochemistry

authors： Dahlman-Wright K,Wright AP,Gustafsson JA

更新日期：1992-09-22 00:00:00

abstract：:Understanding the mechanisms of free energy transfer in metabolism is fundamental to understanding how the chemical forces that sustain the molecular organization of the cell are distributed. Recent studies of molecular motors (1-3) and ATP-driven proton transport (4-6) describe how chemical potential is transferred a...

journal_title：Biochemistry

authors： Wei J,Tang QX,Varlamova O,Roche C,Lee R,Leyh TS

更新日期：2002-07-02 00:00:00

abstract：:Escherichia coli ribonucleotide reductase is an α2β2 complex that catalyzes the conversion of nucleoside 5'-diphosphates (NDPs) to deoxynucleotides (dNDPs). The active site for NDP reduction resides in α2, and the essential diferric-tyrosyl radical (Y(122)(•)) cofactor that initiates transfer of the radical to the act...

journal_title：Biochemistry

authors： Seyedsayamdost MR,Yee CS,Stubbe J

更新日期：2011-03-01 00:00:00

abstract：:The iron-sulfur flavoenzyme adenosine-5'-phosphosulfate (APS) reductase catalyzes a key reaction of the global sulfur cycle by reversibly transforming APS to sulfite and AMP. The structures of the dissimilatory enzyme from Archaeoglobus fulgidus in the reduced state (FAD(red)) and in the sulfite adduct state (FAD-sulf...

journal_title：Biochemistry

authors： Schiffer A,Fritz G,Kroneck PM,Ermler U

更新日期：2006-03-07 00:00:00

abstract：:The interaction of alamethicin with artificial lecithin multilamellar dispersions was investigated by nuclear magnetic resonance (NMR) and Raman spectroscopies. 31P NMR studies revealed perturbation of the lipid head groups in the presence of the icosapeptide. Simulation of the 31P NMR spectra indicated that the obser...

journal_title：Biochemistry

authors： Banerjee U,Zidovetzki R,Birge RR,Chan SI

更新日期：1985-12-17 00:00:00

abstract：:In mesophiles, triosephosphate isomerase (TIM) is an obligated homodimer. We have previously shown that monomeric folding intermediates are common in the chemical unfolding of TIM, where dissociation provides 75% of the overall conformational stability of the dimer. However, analysis of the crystallographic structure ...

journal_title：Biochemistry

authors： Tellez LA,Blancas-Mejia LM,Carrillo-Nava E,Mendoza-Hernández G,Cisneros DA,Fernández-Velasco DA

更新日期：2008-11-04 00:00:00

abstract：:We have detected at least four forms of DNA-dependent ATPase in mouse FM3A cell extracts [Tawaragi, Y., Enomoto, T., Watanabe, Y., Hanaoka, F., & Yamada, M. (1984) Biochemistry 23, 529-533]. The purified fraction of one of the four forms, ATPase B, has been shown to have DNA helicase activity by using a DNA substrate ...

journal_title：Biochemistry

authors： Seki M,Enomoto T,Hanaoka F,Yamada M

更新日期：1987-05-19 00:00:00

abstract：:Human hephaestin (Hp) is a transmembrane protein that has been implicated in duodenal iron export. Mutations in the murine hephaestin gene (sla) produce microcytic, hypochromic anemia that is refractory to oral iron therapy. Hp shares approximately 50% sequence identity with the plasma multicopper ferroxidase cerulopl...

journal_title：Biochemistry

authors： Griffiths TA,Mauk AG,MacGillivray RT

更新日期：2005-11-15 00:00:00

abstract：:The bacterial phosphoenolpyruvate:sugar phosphotransferase system involves a series of reactions in which phosphoprotein intermediates are formed. Histidine-containing protein (HPr) is phosphorylated on the N delta 1 position of the imidazole ring of His15 by enzyme I and acts as a phosphoryl donor to the sugar-specif...

journal_title：Biochemistry

authors： Rajagopal P,Waygood EB,Klevit RE

更新日期：1994-12-27 00:00:00

abstract：:Resonance energy transfer measurements provide a way to estimate distances between chromophores attached to different sites of macromolecules. There are two unknowns involved in resonance energy transfer measurements, the distance between two chromophores and their relative orientation. When static orientational disor...

journal_title：Biochemistry

authors： Wu P,Brand L

更新日期：1992-09-01 00:00:00

abstract：:Affinity-labeling experiments with 125I-endothelin derivatives using bifunctional cross-linking reagents were carried out in an attempt to identify the polypeptide component(s) of the endothelin/sarafotoxin receptors in rat brain tissues. In rat cerebellum, cortex, and caudate putamen, endothelin 1 specifically labele...

journal_title：Biochemistry

authors： Ambar I,Kloog Y,Sokolovsky M

更新日期：1990-07-10 00:00:00

abstract：:Phosphofructokinase from Escherichia coli (EcPFK) is a homotetramer with four active sites and four allosteric sites. Understanding the allosteric activation of EcPFK by MgADP has been complicated by the complex web of possible interactions, including active site homotropic interactions, allosteric site homotropic int...

journal_title：Biochemistry

authors： Fenton AW,Paricharttanakul NM,Reinhart GD

更新日期：2004-11-09 00:00:00

abstract：:Glycine oxidase gene from a strain of Bacillus subtilis was cloned and expressed in Escherichia coli. The purified enzyme was found, by mass spectrometry, to have a protein M(r) of 40763 (value of 40761.6 predicted from DNA sequence) and a FAD prosthetic group M(r) of 785.1 (theoretical value of 785.5). Glycine oxidas...

journal_title：Biochemistry

authors： Jamil F,Gardner QA,Bashir Q,Rashid N,Akhtar M

更新日期：2010-08-31 00:00:00

abstract：:Sequential 1H NMR assignments of a metallothionein from Neurospora crassa have been accomplished by the combined use of COSY, 2QF-COSY, HOHAHA, and rotating-frame NOE experiments. All potentially observable resonances were assigned except for the epsilon-NH3 group of the C-terminal lysine. 1H NOEs, when observed in th...

journal_title：Biochemistry

authors： Malikayil JA,Lerch K,Armitage IM

更新日期：1989-04-04 00:00:00

abstract：:Thrombospondin-1 (TSP1) binding to calreticulin (CRT) on the cell surface signals focal adhesion disassembly, leading to the intermediate adhesive phenotype, cell migration, anoikis resistance, and collagen stimulation. Residues Lys 24 and 32 in TSP1 and amino acids 24-26 and 32-34 in CRT have been shown through bioch...

journal_title：Biochemistry

authors： Yan Q,Murphy-Ullrich JE,Song Y

更新日期：2011-02-01 00:00:00

abstract：:The photolyase/cryptochrome family is a large family of flavoproteins that encompasses DNA repair proteins, photolyases, and cryptochromes that regulate blue-light-dependent growth and development in plants, and light-dependent and light-independent circadian clock setting in animals. Phylogenetic analysis has reveale...

journal_title：Biochemistry

authors： Oztürk N,Kao YT,Selby CP,Kavakli IH,Partch CL,Zhong D,Sancar A

更新日期：2008-09-30 00:00:00

abstract：:Catalase-peroxidases (KatGs) are unique bifunctional heme peroxidases with an additional posttranslationally formed redox-active Met-Tyr-Trp cofactor that is essential for catalase activity. On the basis of studies of bacterial KatGs, controversial mechanisms of hydrogen peroxide oxidation were proposed. The recent di...

journal_title：Biochemistry

authors： Gasselhuber B,Graf MM,Jakopitsch C,Zamocky M,Nicolussi A,Furtmüller PG,Oostenbrink C,Carpena X,Obinger C

更新日期：2016-06-28 00:00:00

abstract：:Nisin is a cationic polycyclic bacteriocin secreted by some lactic acid bacteria. Nisin has previously been shown to permeabilize liposomes. The interaction of nisin was analyzed with liposomes prepared of the zwitterionic phosphatidylcholine (PC) and the anionic phosphatidylglycerol (PG). Nisin induces the release of...

journal_title：Biochemistry

authors： Driessen AJ,van den Hooven HW,Kuiper W,van de Kamp M,Sahl HG,Konings RN,Konings WN

更新日期：1995-02-07 00:00:00

abstract：:Endonexin (annexin IV) is a member of the annexin family of homologous proteins that bind membranes and aggregate vesicles in a calcium-dependent fashion. This study examines the lipid modulation and mechanism of the binding of endonexin to membranes using a fluorescence energy transfer assay to measure bovine endonex...

journal_title：Biochemistry

authors： Junker M,Creutz CE

更新日期：1994-08-02 00:00:00