当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Interaction with the Redox Cofactor MYW and Functional Role of a Mobile Arginine in Eukaryotic Catalase-Peroxidase.

Interaction with the Redox Cofactor MYW and Functional Role of a Mobile Arginine in Eukaryotic Catalase-Peroxidase.

Abstract：

:Catalase-peroxidases (KatGs) are unique bifunctional heme peroxidases with an additional posttranslationally formed redox-active Met-Tyr-Trp cofactor that is essential for catalase activity. On the basis of studies of bacterial KatGs, controversial mechanisms of hydrogen peroxide oxidation were proposed. The recent discovery of eukaryotic KatGs with differing pH optima of catalase activity now allows us to scrutinize those postulated reaction mechanisms. In our study, secreted KatG from the fungus Magnaporthe grisea (MagKatG2) was used to analyze the role of a remote KatG-typical mobile arginine that was shown to interact with the Met-Tyr-Trp adduct in a pH-dependent manner in bacterial KatGs. Here we present crystal structures of MagKatG2 at pH 3.0, 5.5, and 7.0 and investigate the mobility of Arg461 by molecular dynamics simulation. Data suggest that at pH ≥4.5 Arg461 mostly interacts with the deprotonated adduct Tyr. Elimination of Arg461 by mutation to Ala slightly increases the thermal stability but does not alter the active site architecture or the kinetics of cyanide binding. However, the variant Arg461Ala lost the wild-type-typical optimum of catalase activity at pH 5.25 (kcat = 6450 s(-1)) but exhibits a broad plateau between pH 4.5 and 7.5 (kcat = 270 s(-1) at pH 5.5). Moreover, significant differences in the kinetics of interconversion of redox intermediates of wild-type and mutant protein mixed with either peroxyacetic acid or hydrogen peroxide are observed. These findings together with published data from bacterial KatGs allow us to propose a role of Arg461 in the H2O2 oxidation reaction of KatG.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Gasselhuber B,Graf MM,Jakopitsch C,Zamocky M,Nicolussi A,Furtmüller PG,Oostenbrink C,Carpena X,Obinger C

doi

10.1021/acs.biochem.6b00436

subject

Has Abstract

pub_date

2016-06-28 00:00:00

pages

3528-41

issue

25

eissn

0006-2960

issn

1520-4995

journal_volume

55

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Single-strand and double-strand deoxyribonucleic acid breaks produced by several bleomycin analogues.

    abstract：:Production of single-strand breaks (ssb) and double-strand breaks (dsb) of PM2 phage DNA by several structurally related bleomycin (BLM) analogues was studied by gel electrophoresis. BLM A2 and BLM B2 produced a comparable extent of dsb. In various experiments, BLM A2 and BLM B2, at 22-41 ng/mL, degraded 50% of the fo...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00505a001

    authors： Huang CH,Mirabelli CK,Jan Y,Crooke ST

    更新日期：1981-01-20 00:00:00

  • Neurofibrillar tangle surrogates: histone H1 binding to patterned phosphotyrosine peptide nanotubes.

    abstract：:Living cells contain a range of densely phosphorylated surfaces, including phospholipid membranes, ribonucleoproteins, and nucleic acid polymers. Hyperphosphorylated surfaces also accumulate in neurodegenerative diseases as neurofibrillar tangles. We have synthesized and structurally characterized a precisely patterne...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500599a

    authors： Li S,Sidorov AN,Mehta AK,Bisignano AJ,Das D,Childers WS,Schuler E,Jiang Z,Orlando TM,Berland K,Lynn DG

    更新日期：2014-07-08 00:00:00

  • Cyanide binding at the non-heme Fe2+ of the iron-quinone complex of photosystem II: at high concentrations, cyanide converts the Fe2+ from high (S = 2) to low (S = 0) spin.

    abstract：:The primary electron acceptor complex of photosystem II, QAFe2+, can bind a number of small molecules at the iron site, including cyanide [Koulougliotis, D., Kostopoulos, T., Petrouleas, V., & Diner, B. A. (1993) Biochim. Biophys. Acta 1141, 275-282)]. In the presence of NaCN (30-300 mM) at pH 6.5, the reduced state, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00199a014

    authors： Sanakis Y,Petrouleas V,Diner BA

    更新日期：1994-08-23 00:00:00

  • Identification of Factors Complicating Bioluminescence Imaging.

    abstract：:In vivo bioluminescence imaging (BLI) has become a standard, non-invasive imaging modality for following gene expression or the fate of proteins and cells in living animals. Currently, bioluminescent reporters used in laboratories are mostly derivatives of two major luciferase families: ATP-dependent insect luciferase...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b01303

    authors： Yeh HW,Wu T,Chen M,Ai HW

    更新日期：2019-03-26 00:00:00

  • Importance of loops of mitochondrial ADP/ATP carrier for its transport activity deduced from reactivities of its cysteine residues with the sulfhydryl reagent eosin-5-maleimide.

    abstract：:The effects of various compounds such as the transport substrate ADP and the transport inhibitors carboxyatractyloside (CATR) and bongkrekic acid (BKA) on the labeling of cysteine residues in the ADP/ATP carrier of bovine heart submitochondrial particles by the SH reagent eosin-5-maleimide (EMA) were studied. Of the f...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00198a019

    authors： Majima E,Shinohara Y,Yamaguchi N,Hong YM,Terada H

    更新日期：1994-08-16 00:00:00

  • Probing Structural Dynamics and Topology of the KCNE1 Membrane Protein in Lipid Bilayers via Site-Directed Spin Labeling and Electron Paramagnetic Resonance Spectroscopy.

    abstract：:KCNE1 is a single transmembrane protein that modulates the function of voltage-gated potassium channels, including KCNQ1. Hereditary mutations in the genes encoding either protein can result in diseases such as congenital deafness, long QT syndrome, ventricular tachyarrhythmia, syncope, and sudden cardiac death. Despi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00505

    authors： Sahu ID,Craig AF,Dunagan MM,Troxel KR,Zhang R,Meiberg AG,Harmon CN,McCarrick RM,Kroncke BM,Sanders CR,Lorigan GA

    更新日期：2015-10-20 00:00:00

  • Protein dissection of the antiparallel coiled coil from Escherichia coli seryl tRNA synthetase.

    abstract：:The alpha-helices of coiled-coil proteins are predominantly parallel, in contrast to the general preference for an antiparallel orientation of interacting alpha-helices found in globular proteins. One intriguing exception is the antiparallel, two-stranded coiled coil comprising the long helical arm of the bacterial se...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi962391t

    authors： Oakley MG,Kim PS

    更新日期：1997-03-04 00:00:00

  • NMR solution structure of a complex of calmodulin with a binding peptide of the Ca2+ pump.

    abstract：:The three-dimensional structure of the complex between calmodulin (CaM) and a peptide corresponding to the N-terminal portion of the CaM-binding domain of the plasma membrane calcium pump, the peptide C20W, has been solved by heteronuclear three-dimensional nuclear magnetic resonance (NMR) spectroscopy. The structure ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9908235

    authors： Elshorst B,Hennig M,Försterling H,Diener A,Maurer M,Schulte P,Schwalbe H,Griesinger C,Krebs J,Schmid H,Vorherr T,Carafoli E

    更新日期：1999-09-21 00:00:00

  • Impact of actin glutathionylation on the actomyosin-S1 ATPase.

    abstract：:Glutathionylation of intracellular proteins is an established physiological regulator of protein function. In multiple models, including ischemia-reperfusion of the heart, increased oxidative stress results in the glutathionylation of sarcomeric actin. We hypothesized that actin glutathionylation may play a role in th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi900669m

    authors： Pizarro GO,Ogut O

    更新日期：2009-08-11 00:00:00

  • The ice-binding site of sea raven antifreeze protein is distinct from the carbohydrate-binding site of the homologous C-type lectin.

    abstract：:Antifreeze proteins lower the freezing point of their solution by binding to ice and inhibiting its growth. One of several structurally different antifreeze proteins in fishes (type II) is homologous to the carbohydrate-recognition domain of Ca2+-dependent lectins and adopts the same three-dimensional fold. Type II an...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9820513

    authors： Loewen MC,Gronwald W,Sönnichsen FD,Sykes BD,Davies PL

    更新日期：1998-12-22 00:00:00

  • Identification of the amatoxin-binding subunit of RNA polymerase B by affinity labeling experiments. Subunit B3-the true amatoxin receptor protein of multiple RNA polymerase B.

    abstract：:Crude calf thymus DNA-dependent RNA polymerase, RNA polymerase B (ribonucleoside triphosphate: RNA nucleotidyltransferase, EC 2.7.7.6), was incubated with the tritium labeled, potent inhibitor [3H]amanin, in order to form the enzymatically inactive [3H]amanin-polymerase complex ([3H]A-P complex). Subsequent purificati...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00661a013

    authors： Brodner OG,Wieland T

    更新日期：1976-08-10 00:00:00

  • Adenine protonation in domain B of the hairpin ribozyme.

    abstract：:Protein enzymes often use ionizable side chains, such as histidine, for general acid-base catalysis because the imidazole pK(a) is near neutral pH. RNA enzymes, on the other hand, are comprised of nucleotides which do not have apparent pK(a) values near neutral pH. Nevertheless, it has been recently shown that cytidin...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi001976r

    authors： Ravindranathan S,Butcher SE,Feigon J

    更新日期：2000-12-26 00:00:00

  • A-tract polarity dominate the curvature in flanking sequences.

    abstract：:It is well-known, but little understood, that the nucleotide sequences between phased A(4-6)-tracts (at 10-11 bp intervals) have only a slight effect on overall curvature. To explore this phenomenon, we have examined the gel-migration properties of sequences containing both A-tracts as well as G-tracts (i.e., sequence...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi020662w

    authors： Merling A,Sagaydakova N,Haran TE

    更新日期：2003-05-06 00:00:00

  • Three-dimensional structure of the apo form of the N-terminal EGF-like module of blood coagulation factor X as determined by NMR spectroscopy and simulated folding.

    abstract：:The three-dimensional structure of a 42-residue fragment containing the N-terminal EGF-like module of blood coagulation factor X was determined by means of 2D NMR spectroscopy and computer simulation. The spectroscopic data consisted of 370 NOE distances and 27 dihedral angle constraints. These were used to generate p...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00141a004

    authors： Ullner M,Selander M,Persson E,Stenflo J,Drakenberg T,Teleman O

    更新日期：1992-07-07 00:00:00

  • Interaction of the Saccharomyces cerevisiae alpha-factor with phospholipid vesicles as revealed by proton and phosphorus NMR.

    abstract：:Proton and phosphorus-31 nuclear magnetic resonance (1H and 31P NMR) studies of the interaction between a tridecapeptide pheromone, the alpha-factor of Saccharomyces cerevisiae, and sonicated lipid vesicles are reported. 31P NMR studies demonstrate that there is interaction of the peptide with the phosphorus headgroup...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00436a017

    authors： Jelicks LA,Broido MS,Becker JM,Naider FR

    更新日期：1989-05-16 00:00:00

  • Cryoenzymic studies on actomyosin ATPase. Evidence that the degree of saturation of actin with myosin subfragment 1 affects the kinetics of the binding of ATP.

    abstract：:The initial steps of actomyosin subfragment 1 (acto-S1) ATPase (dissociation and binding of ATP) were studied at -15 degrees C with 40% ethylene glycol as antifreeze. The dissociation kinetics were followed by light scattering in a stopped-flow apparatus, and the binding of ATP was followed by the ATP chase method in ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00459a026

    authors： Tesi C,Travers F,Barman T

    更新日期：1990-02-20 00:00:00

  • The crystal structure of a quercetin 2,3-dioxygenase from Bacillus subtilis suggests modulation of enzyme activity by a change in the metal ion at the active site(s).

    abstract：:Common structural motifs, such as the cupin domains, are found in enzymes performing different biochemical functions while retaining a similar active site configuration and structural scaffold. The soil bacterium Bacillus subtilis has 20 cupin genes (0.5% of the total genome) with up to 14% of its genes in the form of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0484421

    authors： Gopal B,Madan LL,Betz SF,Kossiakoff AA

    更新日期：2005-01-11 00:00:00

  • Autocatalytically fragmented light chain of botulinum a neurotoxin is enzymatically active.

    abstract：:The zinc-endopeptidase light chain of botulinum A neurotoxin undergoes autocatalytic fragmentation that is accelerated by the presence of the metal cofactor, zinc [Ahmed, S. A. et al. (2001) J. Protein Chem. 20, 221-231]. We show in this paper that >95% fragmented light chain obtained in the absence of added zinc reta...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi030062c

    authors： Ahmed SA,McPhie P,Smith LA

    更新日期：2003-11-04 00:00:00

  • Purification and characterization of the purE, purK, and purC gene products: identification of a previously unrecognized energy requirement in the purine biosynthetic pathway.

    abstract：:Aminoimidazole riobnucleotide carboxylase, the sixth step in the purine biosynthetic pathway, catalyzes the conversion of aminoimidazole ribonucleotide (AIR) to carboxyaminoimidazole ribonucleotide (CAIR). The gene products of the purE and purK genes (PurE and PurK, respectively) thought to be responsible for this act...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00136a016

    authors： Meyer E,Leonard NJ,Bhat B,Stubbe J,Smith JM

    更新日期：1992-06-02 00:00:00

  • Structural transitions of deoxyribonucleic acid in aqueous electrolyte solutions. II. The role of hydration.

    abstract：:The data and approach reported in paper I (Hanlon et al., 1975, preceding paper) have been used to calculate the fractional changes in secondary structure of calf thymus deoxyribonucleic acid which occur in aqueous solutions as a function of the concentration of NaCl, KCl, LiCl, CsCl, and NH4Cl. There is a continuous ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00679a018

    authors： Wolf B,Hanlon S

    更新日期：1975-04-22 00:00:00

  • The photobleaching sequence of a short-wavelength visual pigment.

    abstract：:The photobleaching pathway of a short-wavelength cone opsin purified in delipidated form (lambda(max) = 425 nm) is reported. The batho intermediate of the violet cone opsin generated at 45 K has an absorption maximum at 450 nm. The batho intermediate thermally decays to the lumi intermediate (lambda(max) = 435 nm) at ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi010387y

    authors： Kusnetzow A,Dukkipati A,Babu KR,Singh D,Vought BW,Knox BE,Birge RR

    更新日期：2001-07-03 00:00:00

  • Structure and polymorphism of saturated monoacid 1,2-diacyl-sn-glycerols.

    abstract：:The 1,2-diacyl-sn-glycerols (1,2-DGs) are the predominant naturally occurring isomer found in cell membranes, lipid droplets, and lipoproteins. They are involved in the metabolism of monoacylglycerols, triacylglycerols, and phospholipids. The 1,2-DGs participate in the activation of protein kinase C, in phosphorylatio...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00500a008

    authors： Kodali DR,Fahey DA,Small DM

    更新日期：1990-12-04 00:00:00

  • NMR identification of protein surfaces using paramagnetic probes.

    abstract：:Paramagnetic agents produce line broadening and thus cancellation of anti phase cross-peak components in two-dimensional correlated nuclear magnetic resonance spectra. The specificity of this effect was examined to determine its utility for identifying surface residues of proteins. Ubiquitin and hen egg white lysozyme...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00495a005

    authors： Petros AM,Mueller L,Kopple KD

    更新日期：1990-10-30 00:00:00

  • An additional H-bond in the alpha 1 beta 2 interface as the structural basis for the low oxygen affinity and high cooperativity of a novel recombinant hemoglobin (beta L105W).

    abstract：:Site-directed mutagenesis has been used to construct three recombinant mutant hemoglobins (rHbs), rHb(beta L105W), rHb(alpha D94A/betaL105W), and rHb(alpha D94A). rHb(beta L105W) is designed to form a new hydrogen bond from beta 105Trp to alpha 94Asp in the alpha(1)beta(2) subunit interface to lower the oxygen binding...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi001115i

    authors： Fang TY,Simplaceanu V,Tsai CH,Ho NT,Ho C

    更新日期：2000-11-14 00:00:00

  • Purification and spectroscopic characterization of photosystem II reaction center complexes isolated with or without Triton X-100.

    abstract：:The pigment composition of the isolated photosystem II reaction center complex in its most stable and pure form currently is a matter of considerable debate. In this contribution, we present a new method based on a combination of gel filtration chromatography and diode array detection to analyze the composition of pho...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi960991m

    authors： Eijckelhoff C,van Roon H,Groot ML,van Grondelle R,Dekker JP

    更新日期：1996-10-01 00:00:00

  • Uptake of cesium ions by human erythrocytes and perfused rat heart: a cesium-133 NMR study.

    abstract：:Cesium-133 NMR studies have been carried out on suspended human erythrocytes and on perfused rat hearts in media containing CsCl. The resulting spectra exhibit two sharp resonances, arising from intra- and extracellular Cs+, separated in chemical shift by 1.0-1.4 ppm. Thus, intra- and extracellular resonances are easi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00410a003

    authors： Davis DG,Murphy E,London RE

    更新日期：1988-05-17 00:00:00

  • Single-channel recordings from purified acetylcholine receptors reconstituted in bilayers formed at the tip of patch pipets.

    abstract：:The channel of the purified acetylcholine receptor from Torpedo californica electric organ reconstituted in lipid vesicles was assayed by direct electrical recording using patch-clamp pipets. High-resistance seals were obtained by gentle suction of vesicles into the pipet or after the formation of lipid bilayers from ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00279a003

    authors： Suarez-Isla BA,Wan K,Lindstrom J,Montal M

    更新日期：1983-05-10 00:00:00

  • Inactivation of human lung tryptase: evidence for a re-activatable tetrameric intermediate and active monomers.

    abstract：:Human lung tryptase (HLT), a trypsin-like serine proteinase stored as an active enzyme in association with heparin in mast cell granules, is released into the extracellular environment when mast cells are activated. Tryptases are unusual in that they form tetramers and bind heparin. As there are no known endogenous tr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi960042t

    authors： Addington AK,Johnson DA

    更新日期：1996-10-22 00:00:00

  • Interspersion of mouse satellite deoxyribonucleic acid sequences.

    abstract：:DNA sequences with homology to the major (A + T)-rich mouse satellite component were localized in CsCl gradients by hybridization with a labeled satellite cRNA probe. Although, as expected, most of the hybridization was to DNA in the satellite-rich shoulder, substantial radioactive cRNA hybridized with DNA from denser...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00518a025

    authors： Stambrook PJ

    更新日期：1981-07-21 00:00:00

  • Inhibition of protein interactions with the beta 2 sliding clamp of Escherichia coli DNA polymerase III by peptides from beta 2-binding proteins.

    abstract：:The sliding clamp of the Escherichia coli replisome is now understood to interact with many proteins involved in DNA synthesis and repair. A universal interaction motif is proposed to be one mechanism by which those proteins bind the E. coli sliding clamp, a homodimer of the beta subunit, at a single site on the dimer...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi036229j

    authors： Wijffels G,Dalrymple BP,Prosselkov P,Kongsuwan K,Epa VC,Lilley PE,Jergic S,Buchardt J,Brown SE,Alewood PF,Jennings PA,Dixon NE

    更新日期：2004-05-18 00:00:00