Abstract：
:In vivo bioluminescence imaging (BLI) has become a standard, non-invasive imaging modality for following gene expression or the fate of proteins and cells in living animals. Currently, bioluminescent reporters used in laboratories are mostly derivatives of two major luciferase families: ATP-dependent insect luciferases and ATP-independent marine luciferases. Inconsistent results of experiments using different bioluminescent reporters, such as Akaluc and Antareas2, have been reported. Herein, we re-examined the inconsistency in several experimental settings and identified the factors, such as ATP dependency, stability in serum, and molecular sizes of luciferases, that contributed to the observed differences. We expect this study will make the research community aware of these factors and facilitate more accurate interpretation of BLI data by considering the nature of each bioluminescent reporter.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Yeh HW,Wu T,Chen M,Ai HWdoi
10.1021/acs.biochem.8b01303subject
Has Abstractpub_date
2019-03-26 00:00:00pages
1689-1697issue
12eissn
0006-2960issn
1520-4995journal_volume
58pub_type
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