Abstract:
:The flavin reductase (FRED) and isobutylamine N-hydroxylase (IBAH) from Streptomyces viridifaciens constitute a two-component, flavin-dependent monooxygenase system that catalyzes the first step in valanimycin biosynthesis. FRED is an oxidoreductase that provides the reduced flavin to IBAH, which then catalyzes the hydroxylation of isobutylamine (IBA) to isobutylhydroxylamine (IBHA). In this work, we used several complementary methods to investigate FAD binding, steady-state and rapid reaction kinetics, and enzyme-enzyme interactions in the FRED:IBAH system. The affinity of FRED for FADox is higher than its affinity for FADred, consistent with its function as a flavin reductase. Conversely, IBAH binds FADred more tightly than FADox, consistent with its role as a monooxygenase. FRED exhibits a strong preference (28-fold) for NADPH over NADH as the electron source for FAD reduction. Isothermal titration calorimetry was used to study the association of FRED and IBAH. In the presence of FAD, either oxidized or reduced, FRED and IBAH associate with a dissociation constant of 7-8 μM. No interaction was observed in the absence of FAD. These results are consistent with the formation of a protein-protein complex for direct transfer of reduced flavin from the reductase to the monooxygenase in this two-component system.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Li H,Forson B,Eckshtain-Levi M,Valentino H,Martín Del Campo JS,Tanner JJ,Sobrado Pdoi
10.1021/acs.biochem.0c00679subject
Has Abstractpub_date
2021-01-12 00:00:00pages
31-40issue
1eissn
0006-2960issn
1520-4995journal_volume
60pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Peptide NH resonances in the 250 MHZ 1H nuclear magnetic resonance (NMR) spectrum of oxytocin in H2O were assigned to specific amino acid residues by the "underwater decoupling" technique (i.e., decoupling from corresponding CalphaH resonances, which are buried beneath the intense water peak). These experiments confir...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00650a025
更新日期:1976-03-09 00:00:00
abstract::The effects of poly(ethylene glycol) (PEG) on sonicated unilamellar vesicles made of phosphatidylcholine have been examined. Stopped-flow and equilibrium data are presented for vesicle aggregation, vesicle leakage, lipid mixing, and aqueous contents mixing. Vesicle aggregation is detected as a monoexponential increase...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00065a024
更新日期:1993-04-13 00:00:00
abstract::We have combined three mutations previously shown to stabilize lambda repressor against thermal denaturation. Two of these mutations are in helix 3, where Gly-46 and Gly-48 have been replaced by alanines [Hecht, M. H., et al. (1986) Proteins: Struct., Funct., Genet. 1, 43-46]. The other mutation, which replaces Tyr-88...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00419a059
更新日期:1988-09-20 00:00:00
abstract::The solution structure of the dicerium(III) complex of the N-terminal domain of calmodulin (Ce2-TR1C hereafter) has been solved employing paramagnetic T1 relaxation enhancements and pseudocontact shifts introduced by the Ce3+ ions, together with conventional NOE constraints. The use of pseudocontact shift constraints ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971022+
更新日期:1997-09-30 00:00:00
abstract::Chymopapain, a cysteine protease of papaya latex, has been purified with the use of fast protein liquid chromatography. Two homogeneous fractions were analyzed for thiol content and thiol reactivity. It was found that peak 1 and peak 2 contained two and three thiol groups, respectively, per mole of enzyme. This result...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00370a024
更新日期:1986-11-04 00:00:00
abstract::Type B streptogramins, such as virginiamycin S (VS), are cyclic hexadepsipeptides, inhibiting protein synthesis in prokaryotes. L-Thr connects a 3-hydroxypicolinyl residue (3-OH-Pic) to the peptide lactone ring. The fluorescence intensity of 3-OH-Pic is strongly increased by chelation to alkaline earth cations or bind...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00243a033
更新日期:1991-07-23 00:00:00
abstract::A new type of thrombin exo-site inhibitor has been designed with enhanced inhibitory potency and increased metabolic stability. With the aid of the model of the structure of the thrombin-hirudin fragment complex [Yue, S.-Y., DiMaio, J., Szewczuk, Z., Purisima, E. O., Ni, F., & Konishi, Y. (1992) Protein Eng. 5, 77-85]...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00153a004
更新日期:1992-09-29 00:00:00
abstract::The interaction of a nitroxide spin-labeled derivative of ouabain with sheep kidney Na,K-ATPase and the motional behavior of the ouabain spin label-Na,K-ATPase complex have been studied by means of electron paramagnetic resonance (EPR) and saturation-transfer EPR (ST-EPR). Spin-labeled ouabain binds with high affinity...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00122a019
更新日期:1992-02-25 00:00:00
abstract::Arginine 54 in subunit I of cytochrome c oxidase from Paracoccus denitrificans interacts with the formyl group of heme a. Mutation of this arginine to methionine (R54M) dramatically changes the spectral properties of heme a and lowers its midpoint redox potential [Kannt et al. (1999) J. Biol. Chem. 274, 37974-37981; L...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002948b
更新日期:2001-05-01 00:00:00
abstract::Activation of the protein tyrosine kinase receptors requires the coupling of ligand binding to a change in both the proximity and orientation of the single transmembrane (TM) helices of receptor monomers to allow transphosphorylation of the receptor kinase domain. We make use of peptides corresponding to the TM and ju...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500327q
更新日期:2014-08-05 00:00:00
abstract::The Met16Phe mutant of the type 1 copper protein pseudoazurin (PACu), in which a phenyl ring is introduced close to the imidazole moiety of the His81 ligand, has been characterized. NMR studies indicate that the introduced phenyl ring is parallel to the imidazole group of His81. The mutation has a subtle effect on the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi030030p
更新日期:2003-06-10 00:00:00
abstract::Hepatic lipase (HL) hydrolysis of phosphatidylcholine (PC) was studied in recombinant high-density lipoprotein particles (r-HDL). r-HDL were made from cholate mixed micelles that contained PC, apo AI, and, in some cases, unesterified cholesterol. r-HDL were characterized using chemical composition, nondenaturing gradi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970356w
更新日期:1997-10-07 00:00:00
abstract::The correlation of the UVRR nuW3 mode with the tryptophan chi(2,1) dihedral angle [Maruyama and Takeuchi (1995) J. Raman Spectrosc. 26, 319; Miura et al. (1989) J. Raman Spectrosc. 20, 667; Takeuchi (2003) Biopolymers 72, 305] has been extended to a full, 360 degrees rotation. The 3-fold periodicity of the relationshi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801293v
更新日期:2009-03-31 00:00:00
abstract::Human acidic mammalian chitinase (AMCase), a member of the family 18 glycosyl hydrolases, is one of the important proteins involved in Th2-mediated inflammation and has been implicated in asthma and allergic diseases. Inhibition of AMCase results in decreased airway inflammation and airway hyper-responsiveness in a mo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0525977
更新日期:2006-04-11 00:00:00
abstract::We have used nucleoside phosphorothioates (NTP alpha S) and a substitution-interference method to identify phosphate oxygens that appear to be important to guanosine cofactor addition in the self-splicing group I intron from Tetrahymena thermophila. For the majority of these phosphate oxygens, however, the effect of N...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00068a001
更新日期:1993-05-04 00:00:00
abstract::The mechanism by which some hydrophobic molecules such as steroids and free fatty acids (FFA) act as noncompetitive inhibitors of the nicotinic acetylcholine receptor (AChR) is still not known. In the present work, we employ Förster resonance energy transfer (FRET) between the intrinsic fluorescence of membrane-bound ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061388z
更新日期:2007-03-20 00:00:00
abstract::Pyrrolocytosine (PC) and 2-aminopurine (2AP) are fluorescent nucleobase analogues of the DNA nucleobases cytosine and adenine, respectively, and form base pairs with guanine and thymine. Both fluorescent nucleobases are used extensively as probes for local structure in nucleic acids as the fluorescence properties of P...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00521
更新日期:2018-07-31 00:00:00
abstract::The structure and dynamic properties of the C-terminal region of the human class alpha glutathione transferase A1-1 have been investigated with high-resolution NMR methods. On the basis of crystallographic and fluorescence measurements, this 13-residue segment of the enzyme is presumed to be disordered in the unligand...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0363329
更新日期:2004-06-15 00:00:00
abstract::The spin label Tempo-maleimide, when "immobilized" in hemoglobin, is shown to exhibit motional fluctuation whose amplitude and/or frequency depend on temperature and solution conditions. These motional fluctuations are observable by several electron spin resonance techniques. For desalted hemoglobin the fluctuations a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00600a014
更新日期:1978-04-04 00:00:00
abstract::The equilibrium binding and association kinetics of the Saccharomyces cerevisiae TATA Binding Protein (TBP) to the E4 and Major Late promoters of adenovirus (TATATATA and TATAAAAG, respectively), have been directly compared by quantitative DNase I titration and quench-flow "footprinting". The equilibrium binding of TB...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981072u
更新日期:1998-11-10 00:00:00
abstract::The egg jelly that encapsulates amphibian eggs is essential for fertilization, but its molecular composition and roles remain largely unknown. We identified a calcium-dependent lectin from the pentraxin superfamily in the egg jelly coat from the South American burrowing frog, Lepidobatrachus laevis. This lectin, jeltr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035314o
更新日期:2003-11-11 00:00:00
abstract::From an evolutionary point of view, plant and animal ferritins arose from a common ancestor, but plant ferritin exhibits different features as compared with the animal analogue. One major difference is that the 4-fold channels naturally occurring in plant ferritin are hydrophilic, whereas the 4-fold channels in animal...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500066m
更新日期:2014-04-15 00:00:00
abstract::The kinetics of the phospholipase A2-catalyzed hydrolysis of bilayer vesicles and mixed micelles of several oxyglycero and thioglycero analogues of phospholipids have been studied. The results with vesicles show that, depending on the source of the enzyme, the rates of hydrolysis of the oxy-containing long-chain phosp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00149a014
更新日期:1992-09-01 00:00:00
abstract::The translation of a small number of mRNAs in mouse SC-1 fibroblasts can be stimulated by cycloheximide, under conditions where the synthesis of most proteins is inhibited. These mRNAs are ordinarily present in small polyribosomes or messenger ribonucleoprotein particles, although the addition of cycloheximide drives ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00356a030
更新日期:1986-04-22 00:00:00
abstract::The flash-induced Fourier transform infrared (FTIR) difference spectrum of the oxygen-evolving Mn cluster upon S1-to-S2 transition (S2/S1 spectrum) was measured using photosystem II (PS II) core complexes of Synechocystis 6803 in which tyrosine residues were specifically labeled with 13C at the ring-4 position. The do...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971760y
更新日期:1997-12-02 00:00:00
abstract::S-Adenosylmethionine decarboxylase (AdoMetDC) is a key enzyme in the polyamine biosynthetic pathway. Inhibition of this pathway and subsequent depletion of polyamine levels is a viable strategy for cancer chemotherapy and for the treatment of parasitic diseases. Substrate analogue inhibitors display an absolute requir...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900590m
更新日期:2009-07-14 00:00:00
abstract::Human apolipoprotein stimulation of sphingomyelin (SM) hydrolysis by sphingomyelinase from human skin fibroblasts has been studied. Apolipoproteins A-I, A-II, B, C-I, and E do not enhance sphingomyelin hydrolysis above control levels. In contrast, apoC-II stimulates sphingomyelin hydrolysis by approximately 2.5-fold. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00363a036
更新日期:1986-07-29 00:00:00
abstract::Corticosteroid binding globulin (CBG), a serum glycoprotein which binds glucocorticoids and progestins with high affinity, is widely distributed throughout the animal world. Although its charge and size characteristics have largely been conserved across species, we found the behavior of CBG in squirrel monkey (Saimiri...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00407a046
更新日期:1988-04-05 00:00:00
abstract::To develop molecules capable of directly probing the catechol binding region of the beta(2)-adrenergic receptor (beta(2)AR), novel benzophenone- and fluorenone-based beta(2)AR antagonists were prepared as potential photoaffinity probes. While the benzophenone-containing ligands bound with relatively modest affinity, o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001342k
更新日期:2000-10-24 00:00:00
abstract::Six affinity reagents containing chemically reactive groups, either on the phosphate residue at the 5'-end or on the 5'- or 3'-end internucleoside phosphate linkages of the oligothymidylate primers, were used to covalently modify the human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT). After covalent ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00065a015
更新日期:1993-04-13 00:00:00