当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > The interface between the EGF2 domain and the protease domain in blood coagulation factor IX contributes to factor VIII binding and factor X activation.

The interface between the EGF2 domain and the protease domain in blood coagulation factor IX contributes to factor VIII binding and factor X activation.

Abstract：

:The light chain of activated factor IX (FIXa) is involved in a number of functional properties, including FIXa enzymatic activity. This suggests the existence of a functional link between the FIXa light chain and the catalytic domain. The FIXa structure includes a few putative interactions between EGF2 and the protease domain. The role thereof has been addressed in this study. Recombinant FIX variants FIX-N92A, FIX-N92H, FIX-Y295A, and FIX-F299A were produced in 293 cells. After activation, the purified mutants were analyzed for a variety of functional parameters. None of these substitutions had a major effect on the interaction with antithrombin or the cleavage of the chromogenic substrate CH(3)SO(2)-d-CHG-Gly-Arg-p-nitroanilide. All FIXa mutants, however, exhibited a reduced level of factor X (FX) activation. Defective proteolytic activity occurred both in the absence and in the presence of activated factor VIII (FVIIIa). All mutants also exhibited a reduced level of FX activation in the absence of phospholipids. This suggests that putative interdomain contacts involving residues Asn(92), Tyr(295), and Phe(299) affect reactivity toward FX. Detailed kinetic studies in the presence of phospholipids and FVIIIa revealed substrate inhibition, particularly for mutants FIXa-N92A and FIXa-N92H. Surface plasmon resonance demonstrated that the same replacements weaken the association with the isolated factor VIII (FVIII) A2 domain and the FVIII light chain. This implies a defect in the formation of the FX-activating complex that is membrane-independent. We conclude that contacts between EGF2 and the protease domain of FIXa are crucial for FIXa enzymatic activity and for the assembly of the FX-activating complex.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Fribourg C,Meijer AB,Mertens K

doi

10.1021/bi060451h

subject

Has Abstract

pub_date

2006-09-05 00:00:00

pages

10777-85

issue

35

eissn

0006-2960

issn

1520-4995

journal_volume

45

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Environmental influences on the in vivo level of intramolecular triplex DNA in Escherichia coli.

    abstract：:We describe an assay for detecting intramolecular triple-stranded DNA in living cells. The assay involves quantitative analysis of the differential photobinding of 4,5',8-trimethylpsoralen to 5'TA and 5'AT dinucleotides in a region of plasmid DNA containing the triplex-forming sequence (GA)7TA(GA)7. Psoralen photobind...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00075a013

    authors： Ussery DW,Sinden RR

    更新日期：1993-06-22 00:00:00

  • The dual-specific active site of 7,8-diaminopelargonic acid synthase and the effect of the R391A mutation.

    abstract：:7,8-diaminopelargonic acid (DAPA) synthase (EC 2.6.1.62) is a pyridoxal phosphate (PLP)-dependent transaminase that catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form DAPA in the antepenultimate step in the biosynthesis of biotin. The wild...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi026339a

    authors： Eliot AC,Sandmark J,Schneider G,Kirsch JF

    更新日期：2002-10-22 00:00:00

  • Zebrafish neuroglobin is a cell-membrane-penetrating globin.

    abstract：:Neuroglobin (Ngb) is a recently discovered vertebrate heme protein that is expressed in the brain and can reversibly bind oxygen. Mammalian Ngb is involved in neuroprotection under oxidative stress conditions, such as ischemia and reperfusion. We previously demonstrated that human ferric Ngb binds to the alpha subunit...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi800286m

    authors： Watanabe S,Wakasugi K

    更新日期：2008-05-13 00:00:00

  • Model for general acid-base catalysis by the hammerhead ribozyme: pH-activity relationships of G8 and G12 variants at the putative active site.

    abstract：:We have used nucleobase substitution and kinetic analysis to test the hypothesis that hammerhead catalysis occurs by a general acid-base mechanism, in which nucleobases are directly involved in deprotonation of the attacking 2'-hydroxyl group and protonation of the 5'-oxygen that serves as the leaving group in the cle...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi047941z

    authors： Han J,Burke JM

    更新日期：2005-05-31 00:00:00

  • Operative Binding of Class I Release Factors and YaeJ Stabilizes the Ribosome in the Nonrotated State.

    abstract：:During translation, the small subunit of the ribosome rotates with respect to the large subunit primarily between two states as mRNA is being translated into a protein. At the termination of bacterial translation, class I release factors (RFs) bind to a stop codon in the A-site and catalyze the release of the peptide ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b00824

    authors： Casy W,Prater AR,Cornish PV

    更新日期：2018-04-03 00:00:00

  • Relaxation kinetics of glutamate dehydrogenase self-association by pressure perturbation.

    abstract：:The kinetics of self-association for beef liver glutamate dehydrogenase (EC 1.4.1.3) have been measured by using pressure perturbation in both the time domain and the frequency domain by monitoring scattered light intensity. The kinetic behavior is entirely consistent with the random self-association model proposed by...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00579a007

    authors： Halvorson HR

    更新日期：1979-06-12 00:00:00

  • Structural analysis of covalently labeled estrogen receptors by limited proteolysis and monoclonal antibody reactivity.

    abstract：:We have used limited proteolysis of affinity-labeled estrogen receptors (ER), coupled with antireceptor antibody immunoreactivity, to assess structural features of ER and the relatedness of ER from MCF-7 human breast cancer and rat uterine cells. MCF-7 ER preparations covalently labeled with [3H]tamoxifen aziridine [(...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00382a043

    authors： Katzenellenbogen BS,Elliston JF,Monsma FJ Jr,Springer PA,Ziegler YS,Greene GL

    更新日期：1987-04-21 00:00:00

  • Behavior of fragmented calcium (II) adenosine triphosphatase from sarcoplasmic reticulum in detergent solution.

    abstract：:The behavior of Ca2+-ATPase from sarcoplasmic reticulum in detergent solution was compared with that of Ca2+-ATPase which had been cleaved in half by limited trypsin digestion. Attempts to dissociate the fragments (I and II) with an excess of detergent micelles demonstrated that fragments I and II are structurally dep...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00612a028

    authors： Rizzolo LJ,Tanford C

    更新日期：1978-09-19 00:00:00

  • Cardiac troponin T forms a tetramer in vitro.

    abstract：:Cardiac troponin T (cTnT) is a myofibrillar protein essential for calcium-dependent contraction. This property has led to functional studies of developmentally expressed cTnT isoforms and mutants identified in patients with hypertrophic cardiomyopathy. The release of cTnT into the serum following myocardial infarction...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi7012596

    authors： Lounes KC,Demeler B,Anderson DE,Gomes AW,Potter JD,Nassar R,Anderson PA

    更新日期：2008-02-19 00:00:00

  • Genetically Encoded Chemical Decaging in Living Bacteria.

    abstract：:We report the genetically encoded chemical decaging strategy for protein activation in living bacterial cells. In contrast to the metabolically labile photocaging groups inside Escherichia coli, our chemical decaging strategy that relies on the inverse electron-demand Diels-Alder (iDA) reaction is compatible with the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b01017

    authors： Liu L,Liu Y,Zhang G,Ge Y,Fan X,Lin F,Wang J,Zheng H,Xie X,Zeng X,Chen PR

    更新日期：2018-01-30 00:00:00

  • Mutagenesis of the uncoupling protein of brown adipose tissue. Neutralization Of E190 largely abolishes pH control of nucleotide binding.

    abstract：:For expression in Saccharomyces cerevisiae the cDNA of the uncoupling protein (UCP) of brown adipose tissue from hamster has been isolated and used to transform yeast cells. Optimized expression conditions yielded 2% of mitochondrial protein as UCP. UCP was isolated, avoiding copurification of ADP/ATP carrier and pori...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi970513r

    authors： Echtay KS,Bienengraeber M,Klingenberg M

    更新日期：1997-07-08 00:00:00

  • Cooperative stabilization of transthyretin by clusterin and diflunisal.

    abstract：:The circulating protein transthyretin (TTR) can unfold, oligomerize, and form highly structured amyloid fibrils that are deposited in tissues, causing organ damage and disease. This pathogenic process is caused by a heritable TTR point mutation in cases of familial TTR-related amyloidosis or wild-type TTR in cases of ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi5011249

    authors： Greene MJ,Klimtchuk ES,Seldin DC,Berk JL,Connors LH

    更新日期：2015-01-20 00:00:00

  • Photo-cross-linking of CRP to nonspecific DNA in the absence of cAMP. DNA interacts with both the N- and C-terminal parts of the protein.

    abstract：:Adenosine cyclic 3',5'-phosphate receptor protein (CRP or CAP) is a regulatory protein involved in the transcription of several operons in Escherichia coli. cAMP-independent, nonspecific complexes of CRP and DNA were investigated by photochemical cross-linking of the protein to nonspecific DNA, whose thymines are subs...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00058a010

    authors： Katouzian-Safadi M,Blazy B,Cremet JY,Le Caer JP,Rossier J,Charlier M

    更新日期：1993-02-23 00:00:00

  • Electron-transferring flavoprotein from pig kidney: flavin analogue studies.

    abstract：:Apo-electron-transferring flavoprotein from pig kidney (apo-ETF) has been prepared by an acid ammonium sulfate procedure and reconstituted with FAD analogues to probe the flavin binding site. The 8-position of the bound flavin is accessible to solvent as judged by the reaction of 8-Cl-FAD-ETF with sodium sulfide and t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00370a050

    authors： Gorelick RJ,Thorpe C

    更新日期：1986-11-04 00:00:00

  • A potent oligosaccharyl transferase inhibitor that crosses the intracellular endoplasmic reticulum membrane.

    abstract：:Recent work has resulted in the development of potent inhibitors of oligosaccharyl transferase (OT), the enzyme that catalyzes the cotranslational glycosylation of asparagine [Hendrickson, T. L., Spencer, J. R., Kato, M., and Imperiali, B. (1996) J. Am. Chem. Soc. 118, 7636-7637; Kellenberger, C., Hendrickson, T. L., ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi982740e

    authors： Eason PD,Imperiali B

    更新日期：1999-04-27 00:00:00

  • Hypertrophic cardiomyopathy-linked mutation D145E drastically alters calcium binding by the C-domain of cardiac troponin C.

    abstract：:The role of the C-domain sites of cardiac troponin C in the modulation of the calcium signal remains unclear. In this study, we investigated the effects of hypertrophic cardiomyopathy-linked mutations A8V, E134D, and D145E in cardiac troponin C on the properties of the C-domain sites. The A8V mutation had essentially ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi100400h

    authors： Swindle N,Tikunova SB

    更新日期：2010-06-15 00:00:00

  • Transthyretin mutation Leu-55-Pro significantly alters tetramer stability and increases amyloidogenicity.

    abstract：:A recently reported variant of human transthyretin (TTR), Leu-55-Pro, implicated as the causative agent in early-onset familial amyloid polyneuropathy was expressed and characterized, and its denaturation pathway and amyloidogenicity were compared to those of wild-type transthyretin. The overlap-extension polymerase c...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00096a024

    authors： McCutchen SL,Colon W,Kelly JW

    更新日期：1993-11-16 00:00:00

  • Interaction of troponin and tropomyosin: spectroscopic and calorimetric studies.

    abstract：:The thermodynamic parameters characterizing the interaction between rabbit fast skeletal muscle troponin and tropomyosin have been determined at 25 degrees C for three solution conditions: buffer containing (A) 1 mM CaCl2, simulating a "turned-on" state, (B) 3 mM MgCl2, simulating a "turned-off" state, and (C) 2 mM et...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00340a040

    authors： Ingraham RH,Swenson CA

    更新日期：1985-09-10 00:00:00

  • Correlations of crystal structures of DNA oligonucleotides with enantioselective recognition by Rh(phen)2phi3+: probes of DNA propeller twisting in solution.

    abstract：:Rh(phen)2phi3+ (phen = 1,10-phenanthroline; phi = 9,10-phenanthrenequinone diimine) avidly binds to DNA via intercalation from the major groove and upon photoactivation produces strand scission with single base 5' asymmetry. Enantiomers of Rh(phen)2phi3+, which lack hydrogen-bonding substituents in ancillary positions...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00180a005

    authors： Campisi D,Morii T,Barton JK

    更新日期：1994-04-12 00:00:00

  • Cofactor Complexes of DesD, a Model Enzyme in the Virulence-related NIS Synthetase Family.

    abstract：:The understudied nonribosomal-peptide-synthetase-independent siderophore (NIS) synthetase family has been increasingly associated with virulence in bacterial species due to its key role in the synthesis of hydroxamate and carboxylate "stealth" siderophores. We have identified a model family member, DesD, from Streptom...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b00899

    authors： Hoffmann KM,Goncuian ES,Karimi KL,Amendola CR,Mojab Y,Wood KM,Prussia GA,Nix J,Yamamoto M,Lathan K,Orion IW

    更新日期：2020-09-22 00:00:00

  • The SH3 domain of alphaII spectrin is a target for the Fanconi anemia protein, FANCG.

    abstract：:The structural protein nonerythroid alpha spectrin (alphaIISp) plays a role in the repair of DNA interstrand cross-links and is deficient in cells from patients with Fanconi anemia (FA), in which there is a defect in ability to repair such cross-links. We have proposed a model in which alphaIISp, whose stability is de...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi801483u

    authors： Lefferts JA,Wang C,Sridharan D,Baralt M,Lambert MW

    更新日期：2009-01-20 00:00:00

  • Structure and energetics of a non-proline cis-peptidyl linkage in a proline-202-->alanine carbonic anhydrase II variant.

    abstract：:The crystal structure of a human carbonic anhydrase II (CAII) variant, cis-proline-202-->alanine (P202A), has been determined at 1.7-A resolution, indicating that the wild-type geometry, including the cis-peptidyl linkage, is retained upon substitution of proline by alanine. The CO2 hydrase activity and affinity for s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00092a003

    authors： Tweedy NB,Nair SK,Paterno SA,Fierke CA,Christianson DW

    更新日期：1993-10-19 00:00:00

  • Bimodal action of alkaline earth cations on Dictyostelium discoideum ribonuclease P activity.

    abstract：:The ribonucleoprotein ribonuclease P (RNase P) cleaves all tRNA precursors endonucleolitically to produce the mature 5'-end. Dictyostelium discoideum RNase P displays an absolute requirement for Mg2+. Only the alkaline earth cations Ca2+, Sr2+, and Ba2+, under appropriate conditions can substitute to some extent for M...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9808971

    authors： Tekos A,Stathopoulos C,Drainas D

    更新日期：1998-11-03 00:00:00

  • Tubulin-G protein association stabilizes GTP binding and activates GTPase: cytoskeletal participation in neuronal signal transduction.

    abstract：:It has been suggested that dimeric tubulin can participate in the signal transduction process through its association with the GTP-binding (G) proteins Gs and Gi1. Using the photoaffinity GTP analog, azidoanilido-GTP, it has been shown that the transfer of nucleotide from tubulin to G alpha s and G alpha i1 is the key...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00198a052

    authors： Roychowdhury S,Rasenick MM

    更新日期：1994-08-16 00:00:00

  • Explanation of pre-steady-state kinetics and decreased burst amplitude of HIV-1 reverse transcriptase at sites of modified DNA bases with an additional, nonproductive enzyme-DNA-nucleotide complex.

    abstract：:The majority of pre-steady-state kinetic investigations with HIV-1 reverse transcriptase (HIV-1 RT) have reported substoichiometric bursts (30-50%) of product formation in the initial reaction cycle. By using quantitative amino acid analysis, we have revised the extinction coefficient of the HIV-1 RT heterodimer and s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi982163u

    authors： Furge LL,Guengerich FP

    更新日期：1999-04-13 00:00:00

  • Characterization of metal ion activation and inhibition of CTP synthetase.

    abstract：:A reinvestigation of the metal ion activation and specificity of CTP synthetase was begun in order to separate effects due to binding of free metal ions and binding of nucleotide-complexed ions. Apo-CTP synthetase was prepared by dialysis against 5 mM EDTA. Analysis of apo-enzyme by atomic absorption spectroscopy reve...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00065a032

    authors： Robertson JG,Villafranca JJ

    更新日期：1993-04-13 00:00:00

  • Functional roles of the invariant aspartic acid 55, tyrosine 306, and aspartic acid 309 in glucoamylase from Aspergillus awamori studied by mutagenesis.

    abstract：:Three mutants, Asp55-Gly, Tyr306-->Phe, and Asp309-->Asn, of Aspergillus awamori glucoamylase (identical to Aspergillus niger glucoamylase) were constructed to elucidate the roles of two conserved regions within fungal glucoamylases. Kinetic studies indicate that both of these regions are closely associated with activ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00055a017

    authors： Sierks MR,Svensson B

    更新日期：1993-02-02 00:00:00

  • Amino acid 305 determines catalytic center accessibility in CYP3A4.

    abstract：:Site-directed mutagenesis has been used to replace alanine 305 with phenylalanine (A305F) and serine (A305S) in the active site of cytochrome P450 3A4 (CYP3A4). Enzyme kinetics for diazepam, erythromycin, nifedipine, and testosterone metabolism have been determined for both mutants and wild-type CYP3A4. The A305F muta...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi992372u

    authors： Fowler SM,Riley RJ,Pritchard MP,Sutcliffe MJ,Friedberg T,Wolf CR

    更新日期：2000-04-18 00:00:00

  • Three-dimensional structure of murine anti-p-azophenylarsonate Fab 36-71. 2. Structural basis of hapten binding and idiotypy.

    abstract：:Comparison between the structures and solvent-accessible surfaces of the antigen-binding fragments of two murine anti-p-azophenylarsonate monoclonal antibodies, one bearing a major cross-reactive idiotype of A/J strain mice (36-71) and one lacking the idiotype (R19.9; Lascombe et al., 1989), highlight the structural b...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00229a023

    authors： Strong RK,Petsko GA,Sharon J,Margolies MN

    更新日期：1991-04-16 00:00:00

  • Binding of protein phosphatase 2A to the L-type calcium channel Cav1.2 next to Ser1928, its main PKA site, is critical for Ser1928 dephosphorylation.

    abstract：:The cAMP-dependent protein kinase (PKA) controls a large number of cellular functions. One critical PKA substrate in the brain and heart is the L-type Ca(2+) channel Ca(v)1.2, the activity of which is upregulated by PKA. The main PKA phosphorylation site is serine 1928 in the central pore forming alpha(1)1.2 subunit o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi051593z

    authors： Hall DD,Feekes JA,Arachchige Don AS,Shi M,Hamid J,Chen L,Strack S,Zamponi GW,Horne MC,Hell JW

    更新日期：2006-03-14 00:00:00