Bimodal action of alkaline earth cations on Dictyostelium discoideum ribonuclease P activity.

abstract：:Photosystem II catalyzes the light-driven oxidation of water and reduction of plastoquinone in oxygenic photosynthesis. The manganese stabilizing protein (MSP) of photosystem II is an extrinsic subunit that plays an important role in catalytic activity. This subunit can be extracted and re-bound to the photosystem II ...

journal_title：Biochemistry

authors： Hutchison RS,Betts SD,Yocum CF,Barry BA

更新日期：1998-04-21 00:00:00

abstract：:Phosphatidylinositol-specific phospholipase C (PLC) enzymes catalyze the hydrolysis of phosphatidylinositol 4,5 bisphosphate in a two step reaction that involves a cyclic intermediate. The PLCbetafamily are activated by both the alpha and betagamma subunits of heterotrimeric G proteins. To determine which catalytic st...

journal_title：Biochemistry

authors： Feng J,Roberts MF,Drin G,Scarlata S

更新日期：2005-02-22 00:00:00

abstract：:We have employed high-performance liquid chromatography (HPLC) to investigate the syntheses of histones H1 and H1o as synchronized cells traverse from mitosis to S phase. Chinese hamster (line CHO) cells were synchronized by mitotic selection, and, at appropriate times, they were pulse labeled for 1 h with [3H]lysine....

journal_title：Biochemistry

authors： D'Anna JA,Thayer MM,Tobey RA,Gurley LR

更新日期：1985-04-09 00:00:00

abstract：:Comparisons of catalytic mechanisms have not previously been performed for homologous enzymes from hyperthermophilic and mesophilic sources. Here, the beta-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus was recombinantly produced in Escherichia coli and shown to have biophyscial and biochemical pr...

journal_title：Biochemistry

authors： Bauer MW,Kelly RM

更新日期：1998-12-08 00:00:00

abstract：:ROS-GC represents a membrane guanylate cyclase subfamily whose distinctive feature is that it transduces diverse intracellularly generated Ca(2+) signals into the production of the second messenger cyclic GMP. An intriguing feature of the first subfamily member, ROS-GC1, is that it is both stimulated and inhibited by ...

journal_title：Biochemistry

authors： Kumar VD,Vijay-Kumar S,Krishnan A,Duda T,Sharma RK

更新日期：1999-09-28 00:00:00

abstract：:The kinetics of the tryptophan fluorescence enhancement that occurs when myofibrils (rabbit psoas) are mixed with Mg-ATP were studied by stopped-flow in different solvents (water, 40% ethylene glycol, 20% methanol) at 4 degrees C. Under relaxing conditions (low Ca(2+)) in water (mu = 0.16 M, pH 7.4) and at high ATP co...

journal_title：Biochemistry

authors： Stehle R,Lionne C,Travers F,Barman T

更新日期：2000-06-27 00:00:00

abstract：:The oxidation of deoxy- and oxyhemerythrin to methemerythrin by Fe(CN)6(3-) has been examined. The first step produces (semi-met)O, a half-oxidized form of hemerythrin which has different spectral and kinetic properties from (semi-met)R which is produced by one-electron reduction of methemerythrin. Further oxidation o...

journal_title：Biochemistry

authors： Bradić Z,Harrington PC,Wilkins RG,Yoneda G

更新日期：1980-09-02 00:00:00

abstract：:Clip domains are structural modules found in arthropod serine proteinases and some proteolytically inactive homologues, which mediate extracellular signaling pathways of development and immunity. While little is known about their structures or functions, clip domains are proposed to be sites for interactions of protei...

journal_title：Biochemistry

authors： Huang R,Lu Z,Dai H,Velde DV,Prakash O,Jiang H

更新日期：2007-10-16 00:00:00

abstract：:The crystal structure of Escherichia coli GAR Tfase at 2.1 A resolution in complex with 10-formyl-5,8,10-trideazafolic acid (10-formyl-TDAF, K(i) = 260 nM), an inhibitor designed to form an enzyme-assembled multisubstrate adduct with the substrate, beta-GAR, was studied to determine the exact nature of its inhibitory ...

journal_title：Biochemistry

authors： Greasley SE,Yamashita MM,Cai H,Benkovic SJ,Boger DL,Wilson IA

更新日期：1999-12-21 00:00:00

abstract：:Thermostable direct hemolysin (TDH) of Vibrio parahemolyticus is a membrane-damaging pore-forming toxin with potent cytolytic/cytotoxic activity. TDH exists as a tetramer consisting of protomers with a core β-sandwich domain, flanked by an 11-amino acid long N-terminal region (NTR). This NTR could not be modeled in th...

journal_title：Biochemistry

authors： Kundu N,Verma P,Kumar A,Dhar V,Dutta S,Chattopadhyay K

更新日期：2020-02-04 00:00:00

abstract：:A synthetic analogue of the tripeptide hemiasterlin, designated HTI-286, depolymerizes microtubules, is a poor substrate for P-glycoprotein, and inhibits the growth of paclitaxel-resistant tumors in xenograft models. Two radiolabeled photoaffinity analogues of HTI-286, designated 4-benzoyl-N,beta,beta-trimethyl-l-phen...

journal_title：Biochemistry

authors： Nunes M,Kaplan J,Wooters J,Hari M,Minnick AA Jr,May MK,Shi C,Musto S,Beyer C,Krishnamurthy G,Qiu Y,Loganzo F,Ayral-Kaloustian S,Zask A,Greenberger LM

更新日期：2005-05-10 00:00:00

abstract：:Kinetic studies of reactions between fluorescein and antifluorescein antibody produced during early, intermediate, and late stages of the immune response have been carried out utilizing both fluorescence intensity and polarization measurements in the static (time constant similar to 5 sec) and in the stopped-flow mode...

journal_title：Biochemistry

authors： Levison SA,Hicks AN,Portmann AJ,Dandliker WB

更新日期：1975-08-26 00:00:00

abstract：:A mouse monoclonal anti-7-(O-carboxymethyl)oximinoestradiol antibody was photoaffinity labeled with two cross-reactive 6alpha- and 6beta-(5-azido-2-nitrobenzoyl)amido[17alpha-3H]estradiol photoreagents (6alpha- and 6beta-ANBA-[17alpha-3H]estradiol). Covalently bound radioactivity was found exclusively on the light cha...

journal_title：Biochemistry

authors： Rousselot P,Mappus E,Blachère T,de Ravel MR,Grenot C,Tonnelle C,Cuilleron CY

更新日期：1997-06-24 00:00:00

abstract：:The amino acids (residues 39-51) responsible for the interaction between the first intracellular loop (iLP1) of the human prostacyclin receptor (IP) and G alpha s protein have been identified [Zhang, L., Huang, G., Wu, J., and Ruan, K. H. (2005) Biochemistry 44, 11389-11401]. To further characterize the structural/fun...

journal_title：Biochemistry

authors： Zhang L,Wu J,Ruan KH

更新日期：2006-02-14 00:00:00

abstract：:The reduction kinetics of cytochrome P-450 are known to be biphasic, with a rapid initial phase and a slower subsequent phase, both of which appear linear in semilogarithmic plots. The present report demonstrates that these biphasic reduction kinetics can be described in terms of a preequilibrium between high- and low...

journal_title：Biochemistry

authors： Backes WL,Sligar SG,Schenkman JB

更新日期：1982-03-16 00:00:00

abstract：:A procedure for the preparation of coupling factor 1 (F1) from Escherichia coli lacking subunits delta and epsilon is described. Using chloroform and dimethyl sulfoxide, we can isolate F1 containing only subunits alpha, beta, and gamma [F1(alpha beta gamma)] directly from membrane vesicles in 10-mg quantities. Pure an...

journal_title：Biochemistry

authors： Tuttas-Dörschug R,Hanstein WG

更新日期：1989-06-13 00:00:00

abstract：:The fungal neurotoxin alpha-cyclopiazonic acid (CPA), a nanomolar inhibitor of Ca(2+)-ATPase with a unique pentacyclic indole tetramic acid scaffold, is assembled by a three enzyme pathway CpaS, CpaD, and CpaO in Aspergillus sp. We recently characterized the first pathway-specific enzyme CpaS, a hybrid two module poly...

journal_title：Biochemistry

authors： Liu X,Walsh CT

更新日期：2009-11-24 00:00:00

abstract：:myo-Inositol oxygenase (MIOX) uses iron as its cofactor and dioxygen as its cosubstrate to effect the unique, ring-cleaving, four-electron oxidation of its cyclohexan-(1,2,3,4,5,6-hexa)-ol substrate to d-glucuronate. The nature of the iron cofactor and its interaction with the substrate, myo-inositol (MI), have been p...

journal_title：Biochemistry

authors： Xing G,Hoffart LM,Diao Y,Prabhu KS,Arner RJ,Reddy CC,Krebs C,Bollinger JM Jr

更新日期：2006-05-02 00:00:00

abstract：:Glucarate dehydratase (GlucD) from Pseudomonas putida catalyzes the dehydration of both (D)-glucarate and (L)-idarate to 3-deoxy-(L)-threo-2-hexulosarate as well as their epimerization. (D)-[6-13C]Glucarate and (L)-[6-13C]idarate have been synthesized for use in continuous assay of the reactions catalyzed by GlucD by ...

journal_title：Biochemistry

authors： Palmer DR,Hubbard BK,Gerlt JA

更新日期：1998-10-13 00:00:00

abstract：:Three tyrosine-to-phenylalanine mutants of ribonuclease A (Y25F, Y92F, and Y97F) are investigated for their enzymatic activities, molecular stabilities, and unfolding/refolding kinetics. These mutants exhibit 80, 90, and 80%, respectively, of the catalytic activity of the wild-type enzyme. Thermal, Gdn.HCl, and pH tra...

journal_title：Biochemistry

authors： Juminaga D,Wedemeyer WJ,Garduño-Júarez R,McDonald MA,Scheraga HA

更新日期：1997-08-19 00:00:00

abstract：:The early steps of the proposed mechanistic pathway for dehydroquinate synthase have been probed with a series of substrate analogues. These analogues, 3-9, are structurally prohibited from undergoing the beta-elimination of inorganic phosphate that represents the committed step in the conversion of the substrate 3-de...

journal_title：Biochemistry

authors： Bender SL,Widlanski T,Knowles JR

更新日期：1989-09-19 00:00:00

abstract：:Many naturally occurring RNA structures contain single mismatches. However, the algorithms currently used to predict RNA structure from sequence rely on a minimal set of data for single mismatches, most of which occur rather infrequently in nature. As a result, several approximations and assumptions are used to predic...

journal_title：Biochemistry

authors： Davis AR,Znosko BM

更新日期：2007-11-20 00:00:00

abstract：:Chloramphenicol acetyltransferase (CAT) reversibly transfers an acetyl group between CoA and the 3-hydroxyl of either chloramphenicol (Cm) or 1-acetylchloramphenicol (1AcCm). The products of the forward reactions, 3-acetylchloramphenicol (3-AcCm) and 1,3-diacetylchloramphenicol (1,3Ac2-Cm), are the substrates for the ...

journal_title：Biochemistry

authors： Murray IA,Derrick JP,White AJ,Drabble K,Wharton CW,Shaw WV

更新日期：1994-08-23 00:00:00

abstract：:Polyhydroxybutyrate (PHB) synthases catalyze the conversion of beta-hydroxybutyryl coenzyme A (HBCoA) to PHB. These enzymes require an active site cysteine nucleophile for covalent catalysis. A protein BLASTp search using the Class III Chromatium vinosum synthase sequence reveals high homology to prokaryotic lipases w...

journal_title：Biochemistry

authors： Jia Y,Kappock TJ,Frick T,Sinskey AJ,Stubbe J

更新日期：2000-04-11 00:00:00

abstract：:Phosphoenolthiopyruvate, the analogue of phosphoenolpyruvate in which the bridging oxygen of the phosphate ester is replaced by sulfur, has been synthesized from methyl acrylate and dimethyl (chlorothio)phosphonate. The compound is a substrate for alkaline phosphatase, pyruvate kinase, enolase, and phosphoenolpyruvate...

journal_title：Biochemistry

authors： Sikkema KD,O'Leary MH

更新日期：1988-02-23 00:00:00

abstract：:The genome of all retroviruses consists of two identical RNAs noncovalently linked near their 5' end. Dimerization of genomic RNA is thought to modulate several steps in the retroviral life cycle, such as recombination, translation, and encapsidation. The kissing-loop model of HIV-1 genome dimerization [Laughrea, M., ...

journal_title：Biochemistry

authors： Laughrea M,Jetté L

更新日期：1996-02-06 00:00:00

abstract：:Two new inhibitors in which the terminal α-carboxyl groups of Z-Ala-Ala-Phe-COOH and Z-Ala-Pro-Phe-COOH have been replaced with a proton to give Z-Ala-Ala-Phe-H and Z-Ala-Pro-Phe-H, respectively, have been synthesized. Using these inhibitors, we estimate that for α-chymotrypsin and subtilisin Carlsberg the terminal ca...

journal_title：Biochemistry

authors： Petrillo T,O'Donohoe CA,Howe N,Malthouse JP

更新日期：2012-08-07 00:00:00

abstract：:Complex I, a key component of the mitochondrial respiratory chain, exhibits diminished activity as a result of cardiac ischemia/reperfusion. Cardiac ischemia/reperfusion is associated with increases in the levels of mitochondrial Ca(2+) and pro-oxidants. In the current in vitro study, we sought evidence for a mechanis...

journal_title：Biochemistry

authors： Sadek HA,Szweda PA,Szweda LI

更新日期：2004-07-06 00:00:00

abstract：:A novel cationic lipid was separated from bovine brain white matter by a series of chromatographies on carboxymethyl-Sephadex and silica gel in chloroform and methanol. Its structure was identified unambiguously as de-N-acetyllactotriaosylceramide (deNAcLc(3)Cer) by mass spectrometry and (1)H NMR. The natural occurren...

journal_title：Biochemistry

authors： Hikita T,Tadano-Aritomi K,Iida-Tanaka N,Ishizuka I,Hakomori S

更新日期：2005-07-12 00:00:00

abstract：:Human Monocyte Chemotactic Protein (MCP)-2 has originally been isolated from stimulated osteosarcoma cells as a chemokine coproduced with MCP-1 and MCP-3. Here, a 5'-end extended MCP-2 cDNA was cloned from a human testis cDNA library. It encoded a 76 residue MCP-2 protein, but differed from the reported bone marrow-de...

journal_title：Biochemistry

authors： Van Coillie E,Proost P,Van Aelst I,Struyf S,Polfliet M,De Meester I,Harvey DJ,Van Damme J,Opdenakker G

更新日期：1998-09-08 00:00:00