Abstract:
:Photosystem II catalyzes the light-driven oxidation of water and reduction of plastoquinone in oxygenic photosynthesis. The manganese stabilizing protein (MSP) of photosystem II is an extrinsic subunit that plays an important role in catalytic activity. This subunit can be extracted and re-bound to the photosystem II reaction center. Extraction is associated with decreased stability of manganese binding by the enzyme and by loss in high rates of oxygen evolution activity; reconstitution reverses these phenomena. Since little is known about the assembly of complex membrane proteins, we have employed isotope editing and vibrational spectroscopy to obtain information about any changes in secondary structure that occur in MSP upon functional reconstitution to photosystem II. The spectroscopic data obtained are consistent with substantial changes in conformation when MSP binds to photosystem II; approximately 30-40% of the peptide backbone undergoes a change in secondary structure. These conclusions were reached by comparing different aliquots, before and after binding, of the same 13[C]MSP sample. Analysis of amide I band line shapes through Fourier deconvolution and nonlinear regression suggests that binding of MSP to photosystem II is associated with a decrease in random structure and an increase in beta-sheet content. We conclude that binding of MSP to the reaction center can induce folding of MSP. Our results also indicate that, in solution, MSP can sample a variety of conformational states, which differ in hydrogen bonding of the peptide backbone.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Hutchison RS,Betts SD,Yocum CF,Barry BAdoi
10.1021/bi9724467subject
Has Abstractpub_date
1998-04-21 00:00:00pages
5643-53issue
16eissn
0006-2960issn
1520-4995pii
bi9724467journal_volume
37pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Ultraviolet resonance Raman (UV RR) spectra are reported for ferricytochrome c from tuna and horse heart at pH 1.6, 7, 10, and 13, representing distinct conformational states of the protein (states II, III, IV, and V, respectively). The spectra were obtained with pulsed laser excitation at 200 and 218 nm, via H2 Raman...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00339a035
更新日期:1985-08-27 00:00:00
abstract::The interaction of heparan sulfate with different ligand proteins depends on the precise location of O-sulfate groups in the polysaccharide chain. We have previously shown that overexpression in human kidney 293 cells of a mouse mastocytoma 2-O-sulfotransferase (2-OST), previously thought to catalyze the transfer of s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002926p
更新日期:2001-05-08 00:00:00
abstract::Protein aggregation is associated with a wide range of diseases, and molecular probes that are able to detect a diversity of misfolded protein assemblies are of great importance. The identification of prefibrillar states preceding the formation of well-defined amyloid fibrils is of particular interest both because of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi100922r
更新日期:2010-08-17 00:00:00
abstract::Loop 181-197 of human thymidylate synthase (hTS) populates two conformational states. In the first state, Cys195, a residue crucial for catalytic activity, is in the active site (active conformer); in the other conformation, it is about 10 A away, outside the active site (inactive conformer). We have designed and expr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi7019386
更新日期:2008-04-22 00:00:00
abstract::The organometallic anticancer agent titanocene dichloride, Cp(2)TiCl(2), is now in phase II clinical trials as an anticancer drug, but its mechanism of action is poorly understood. We show here that the interactions of Cp(2)TiCl(2) with human serum transferrin (hTF) and that of Ti(2)-hTF with adenosine triphosphate (A...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000798z
更新日期:2000-08-22 00:00:00
abstract::5-Iodonaphthyl 1-azide (INA) has been previously shown to selectively label, on photolysis, only those proteins in contact with the membrane lipids. Low concentrations (less than 10 microM) of INA added to rat ovarian plasma membranes induced, on photoactivation, a selective and complete loss of the response of the ad...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00298a016
更新日期:1984-01-31 00:00:00
abstract::RPE65, a protein expressed in cells of the retinal pigment epithelium of the eye, is essential for the synthesis by isomerohydrolase of 11-cis-retinal, the chromophore of rod and cone opsins. Recent work has established that RPE65 is a retinyl ester binding protein, and as all-trans-retinyl esters are the substrate fo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0505363
更新日期:2005-07-26 00:00:00
abstract::Rabbit muscle lactate dehydrogenase (LDH) was coupled to Sepharose in such a way that each molecule is expected to be attached via only one subunit. Dissociation of the bound active enzyme by several methods all yielded immobilized subunit derivatives which were inactive. These derivatives were capable of regenerating...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00664a013
更新日期:1976-09-21 00:00:00
abstract::Stopped-flow measurements have been employed to study the kinetics of the conformational changes in TetR (B) induced by tetracycline binding with and without Mg(2+) ions. Result of stopped-flow fluorometry measurements at pH 8.0 indicate conformational changes in the helix-turn-helix motif in the N-terminal domain and...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048548w
更新日期:2005-01-25 00:00:00
abstract::JZTX-XI is a peptide toxin isolated from the venom of the Chinese spider Chilobrachys jingzhao. It contains 34 residues including six cysteine residues with disulfide bridges linked in the pattern of I-IV, II-V, and III-VI. Using 3'- and 5'-RACE methods, the full-length cDNA was identified as encoding an 86-residue pr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061457+
更新日期:2006-12-26 00:00:00
abstract::The single-subunit RNA polymerases make up a widespread family of proteins found in phage, mitochondria, and chloroplasts. Unlike the phage RNAPs, the eukaryotic RNAPs require accessory factors to melt their promoters and diverge from the phage RNAPs in the regions where functions associated with promoter melting in t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300074j
更新日期:2012-05-08 00:00:00
abstract::The structures of synthetic peptides corresponding to regions of putative secondary structure in the cellular prion protein PrPC were studied as models for the conformational transition that features in the formation of the pathogenic isoform, PrPSc. Transgenetic studies argue that these PrP isoforms interact during t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00013a006
更新日期:1995-04-04 00:00:00
abstract::Protein carboxyl methyltransferases (EC 2.1.1.77) that catalyze the transfer of a methyl group from S-adenosylmethionine to L-isoaspartyl and D-aspartyl residues in a variety of peptides and proteins are widely, but not universally, distributed in nature. These enzymes can participate in the repair of damaged proteins...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00092a020
更新日期:1993-10-19 00:00:00
abstract::The duplex genome of Escherichia coli virus M13mp10 was modified at a unique site to contain N-(deoxyguanosin-8-yl)-4-aminobiphenyl (dG8-ABP), the major carcinogen-DNA adduct of the human bladder carcinogen 4-aminobiphenyl. A tetradeoxynucleotide containing a single dG8-ABP residue was synthesized by reacting 5'-d(TpG...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00385a019
更新日期:1987-06-02 00:00:00
abstract::The membrane-bound state of the gene 9 minor coat protein of bacteriophage M13 was studied in various membrane-mimicking systems, including organic solvents, detergent micelles, and phospholipid bilayers. For this purpose we determined the conformational and aggregational properties of the chemically synthesized prote...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981149e
更新日期:1999-01-19 00:00:00
abstract::Pharaonis halorhodopsin (phR), the light-driven chloride ion pump from Natronobacterium pharaonis with C-terminal histidine tag, was expressed in Escherichia coli cells. The protein was solubilized with 0.1% n-dodecyl beta-D-maltopyranoside and purified with a nickel column. Removal of Cl- from the medium yields blue ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011788g
更新日期:2002-02-19 00:00:00
abstract::The equilibrium binding of influenza virus hemagglutinin to derivatives of its cell-surface ligand, sialic acid, was measured by nuclear magnetic resonance (NMR) spectroscopy. Binding was quantified by observing perturbations of sialic acid resonances in the presence of protein. The major perturbation observed was a c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00447a018
更新日期:1989-10-17 00:00:00
abstract::The catalytic activity and inhibitor binding energetics of enzymes are often pH-dependent properties. Aspartic proteases comprise an important class of enzyme targets for structure-based drug design. We have performed a complete thermodynamic study of pepstatin binding to plasmepsin II, an aspartic proteinase found in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971550l
更新日期:1997-12-23 00:00:00
abstract::Cell-signaling peptides have been extensively used to transport functional molecules across the plasma membrane into living cells. These peptides consist of a hydrophobic sequence and a cationic nuclear localization sequence (NLS). It has been assumed that the hydrophobic region penetrates the hydrophobic lipid bilaye...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061895g
更新日期:2007-01-30 00:00:00
abstract::Escherichia coli tyrosine kinase (Etk) regulates the export of pathogenic capsular polysaccharide (CPS) by intermolecularly autophosphorylating its C-terminal tyrosine cluster. The kinase Etk, however, needs to be first activated by the intramolecular phosphorylation of a tyrosine residue, Y574, next to the active sit...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900811p
更新日期:2009-08-25 00:00:00
abstract::This paper reports on NMR-molecular mechanics structural studies of the (-)-trans-anti-[MC]dG adduct positioned opposite dC in the sequence context of the d(C1-C2-A3-T4-C5-[MC]G6-C7-T8-A9-C10-C11).d(G12-G13-T14++ +-A15-G16-C17-G18- A19-T20-G21-G22) duplex [designated (-)-trans-anti-[MC]dG.dC 11-mer duplex]. This adduc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00018a029
更新日期:1995-05-09 00:00:00
abstract::In the accompanying paper [Suzuki, A., Yamazaki, M., & Ito, T. (1996) Biochemistry 35, 5238-5244], we presented a quantitative phase diagram of actin filament ( (F-actin) described with the F-actin concentration and delta chi value which characterizes the affinity of F-actin with solvent. The phase diagram shows that ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9526948
更新日期:1996-04-23 00:00:00
abstract::Data are presented which suggest that photosystem II dependent hydrogen ion accumulation and ATP synthesis can occur only after the lamellar membranes have undergone a conformational change. This membrane conformational change is detected by the electron transport dependent incorporation of diazonium benzene[35S]sulfo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00691a008
更新日期:1975-10-07 00:00:00
abstract::We have investigated the topology of the band 3 protein of the human erythrocyte membrane by a combination of chemical labeling and proteolytic cleavage. The N-terminal third of the membrane-bound domain of band 3 is a 17 000-dalton chymotryptic fragment that is known to traverse the membrane an odd number of times. A...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00321a024
更新日期:1984-12-18 00:00:00
abstract::The pivotal role of the Arg-Gly-Asp (RGD) peptide motif in integrin-mediated cell adhesive interactions with extracellular matrix and plasma proteins stimulated the present design of nonpeptidic mimetics of this sequence. To probe the structural requirements for RGD recognition by integrins, we designed various struct...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00055a002
更新日期:1993-02-02 00:00:00
abstract::The preferential interactions of proteins with solvent components were studied in concentrated aqueous solutions of the sulfate, acetate, and chloride salts of Mg2+, Ba2+, Ca2+, Mn2+ and Ni2+ [except for CaSO4, BaSO4, Mn-(OAc)2, and Ni(OAc)2], and results were compared with those of the Na+ salts. It was found that, f...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00320a004
更新日期:1984-12-04 00:00:00
abstract::The molecular weight of phosphophoryn, an acidic phosphoprotein unique to dentin matrix, has been difficult to determine because of a combination of neutral protease activities in this tissue and the intrinsic high charge density of the molecule. In this study, bovine dentin phosphophoryn (BDPP) was isolated by a proc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00287a025
更新日期:1983-08-30 00:00:00
abstract::The melanocortin-4 (MC4) receptor is a potential therapeutic target for obesity and cachexia, for which nonpeptide agonists and antagonists are being developed, respectively. The aim of this study was to identify molecular interactions between the MC4 receptor and nonpeptide ligands, and to compare the mechanism of bi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051316s
更新日期:2005-11-08 00:00:00
abstract::The dioxygen stretch bands in infrared spectra for solutions of oxy species of human hemoglobin A and its separated subunits, human mutant hemoglobin Zurich (beta 63His to Arg), rabbit hemoglobin, lamprey hemoglobin, sperm whale myoglobin, bovine myoglobin, and a sea worm chlorocruorin are examined. Each protein exhib...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00389a016
更新日期:1987-07-28 00:00:00
abstract::Mitochondria isolated from coenzyme Q deficient yeast cells had no detectable NADH:cytochrome c reductase or succinate:cytochrome c reductase activity but contained normal amounts of cytochromes b and c1 by spectral analysis. Addition of the exogenous coenzyme Q derivatives including Q2, Q6, and the decyl analogue (DB...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00354a031
更新日期:1986-03-25 00:00:00