Coenzyme Q analogues reconstitute electron transport and proton ejection but not the antimycin-induced "red shift" in mitochondria from coenzyme Q deficient mutants of the yeast Saccharomyces cerevisiae.


:Mitochondria isolated from coenzyme Q deficient yeast cells had no detectable NADH:cytochrome c reductase or succinate:cytochrome c reductase activity but contained normal amounts of cytochromes b and c1 by spectral analysis. Addition of the exogenous coenzyme Q derivatives including Q2, Q6, and the decyl analogue (DB) restored the rate of antimycin- and myxothiazole-sensitive cytochrome c reductase with both substrates to that observed with reduced DBH2. Similarly, addition of these coenzyme Q analogues increased 2-3-fold the rate of cytochrome c reduction in mitochondria from wild-type cells, suggesting that the pool of coenzyme Q in the membrane is limiting for electron transport in the respiratory chain. Preincubation of mitochondria from the Q-deficient yeast cells with DBH2 at 25 degrees C restored electrogenic proton ejection, resulting in a H+/2e- ratio of 3.35 as compared to a ratio of 3.22 observed in mitochondria from the wild-type cell. Addition of succinate and either coenzyme Q6 or DB to mitochondria from the Q-deficient yeast cells resulted in the initial reduction of cytochrome b followed by a slow reduction of cytochrome c1 with a reoxidation of cytochrome b. The subsequent addition of antimycin resulted in the oxidant-induced extrareduction of cytochrome b and concomitant oxidation of cytochrome c1 without the "red" shift observed in the wild-type mitochondria. Similarly, addition of antimycin to dithionite-reduced mitochondria from the mutant cells did not result in a red shift in the absorption maximum of cytochrome b as was observed in the wild-type mitochondria in the presence or absence of exogenous coenzyme Q analogues.(ABSTRACT TRUNCATED AT 250 WORDS)






Beattie DS,Clejan L




Has Abstract


1986-03-25 00:00:00












  • Kinetic, Raman, NMR, and site-directed mutagenesis studies of the Pseudomonas sp. strain CBS3 4-hydroxybenzoyl-CoA thioesterase active site.

    abstract::4-Hydroxybenzoyl-coenzyme A (4-HBA-CoA) thioesterase catalyzes the hydrolysis of 4-HBA-CoA to 4-hydroxybenzoate and CoA. X-ray crystallographic analysis of the liganded enzyme has shown that the benzoyl thioester and pantetheine moieties of the substrate ligand are bound in a narrow crevice while the nucleotide moiety...


    pub_type: 杂志文章


    authors: Zhuang Z,Song F,Zhang W,Taylor K,Archambault A,Dunaway-Mariano D,Dong J,Carey PR

    更新日期:2002-09-17 00:00:00

  • Determination of the three-dimensional structure of the bifunctional alpha-amylase/trypsin inhibitor from ragi seeds by NMR spectroscopy.

    abstract::The three-dimensional structure of the bifunctional alpha-amylase/trypsin inhibitor (RBI) from seeds of ragi (Eleusine coracana Gaertneri) has been determined in solution using multidimensional 1H and 15N NMR spectroscopy. The inhibitor consists of 122 amino acids, with 5 disulfide bridges, and belongs to the plant al...


    pub_type: 杂志文章


    authors: Strobl S,Mühlhahn P,Bernstein R,Wiltscheck R,Maskos K,Wunderlich M,Huber R,Glockshuber R,Holak TA

    更新日期:1995-07-04 00:00:00

  • Selective cytotoxicity of rhodium metalloinsertors in mismatch repair-deficient cells.

    abstract::Mismatches in DNA occur naturally during replication and as a result of endogenous DNA damaging agents, but the mismatch repair (MMR) pathway acts to correct mismatches before subsequent rounds of replication. Rhodium metalloinsertors bind to DNA mismatches with high affinity and specificity and represent a promising ...


    pub_type: 杂志文章


    authors: Ernst RJ,Komor AC,Barton JK

    更新日期:2011-12-20 00:00:00

  • Kinetic Landscape of a Peptide Bond-Forming Prolyl Oligopeptidase.

    abstract::Prolyl oligopeptidase B from Galerina marginata (GmPOPB) has recently been discovered as a peptidase capable of breaking and forming peptide bonds to yield a cyclic peptide. Despite the relevance of prolyl oligopeptidases in human biology and disease, a kinetic analysis pinpointing rate-limiting steps for a member of ...


    pub_type: 杂志文章


    authors: Czekster CM,Naismith JH

    更新日期:2017-04-18 00:00:00

  • GTP hydrolysis of cell division protein FtsZ: evidence that the active site is formed by the association of monomers.

    abstract::The essential prokaryotic cell division protein FtsZ is a tubulin homologue that forms a ring at the division site. FtsZ forms polymers in a GTP-dependent manner. Recent biochemical evidence has shown that FtsZ forms multimeric structures in vitro and in vivo and functions as a self-activating GTPase. Structural analy...


    pub_type: 杂志文章


    authors: Scheffers DJ,de Wit JG,den Blaauwen T,Driessen AJ

    更新日期:2002-01-15 00:00:00

  • Solution structure of a DNA dodecamer containing the anti-neoplastic agent arabinosylcytosine: combined use of NMR, restrained molecular dynamics, and full relaxation matrix refinement.

    abstract::The effect of araC incorporation into the dodecamer duplex [d(CGCGAATT) (araC)d(GCG)]2 was examined by comparing its nuclear magnetic resonance (NMR)-determined solution structure with that of the control duplex d[(CGCGAATTCGCG)]2. 1H and 31P resonances in both duplexes were assigned using a combination of 2-D 1H NMR ...


    pub_type: 杂志文章


    authors: Schweitzer BI,Mikita T,Kellogg GW,Gardner KH,Beardsley GP

    更新日期:1994-09-27 00:00:00

  • Characterization of Acyl Carrier Protein-Dependent Glycosyltransferase in Mitomycin C Biosynthesis.

    abstract::Mitomycins make up a group of antitumor natural products that are biosynthesized from aminohydroxybenzoic acid (AHBA) and N-acetylglucosamine (GlcNAc). While the biosynthetic gene cluster was reported two decades ago, the mechanism by which the two building blocks, AHBA and GlcNAc, are coupled during biosynthesis rema...


    pub_type: 杂志文章


    authors: Nguyen HP,Yokoyama K

    更新日期:2019-06-25 00:00:00

  • Structural analysis of ribosomal protein L7/L12 by the heterobifunctional cross-linker 4-(6-formyl-3-azidophenoxy)butyrimidate.

    abstract::The structure of the dimeric form of the protein L7/L12 from ribosomes from Escherichia coli was studied by using the heterobifunctional cross-linker 4-(6-formyl-3-azidophenoxy)butyrimidate. The imidate group of the cross-linker reacts very specifically with Lys-51 of L7/L12. Subsequent cross-linking of this modified ...


    pub_type: 杂志文章


    authors: Maassen JA,Schop EN,Möller W

    更新日期:1981-02-17 00:00:00

  • Exchange mechanisms for hydrogen bonding protons of cytidylic and guanylic acids.

    abstract::The pH dependence of buffer catalysis of exchange of the C-4 amino protons of cyclic cytosine 2',3'-monophosphate (cCMP) and the N-1 proton of cyclic guanosine 2',3'-monophosphate (cGMP) conforms to an exchange mechanism, in which protonation of the nucleobases at C(N-3) AND G(N-7) establishes the important intermedia...


    pub_type: 杂志文章


    authors: McConnell B

    更新日期:1978-07-25 00:00:00

  • Structure of two subfractions of normal porcine (Sus domesticus) serum low-density lipoproteins. X-ray small-angle scattering studies.

    abstract::Two subfractions of low-density lipoproteins (LDL) were isolated from normal pig (Sus domesticus) serum by a combined method including precipitation, ultracentrifugation, and gel chromatography. The fractions recovered from the buoyant density ranges 1.020-1.050 and 1.050-1.090 g/mL, denoted as LDL1 and LDL2, respecti...


    pub_type: 杂志文章


    authors: Jürgens G,Knipping GM,Zipper P,Kayushina R,Degovics G,Laggner P

    更新日期:1981-05-26 00:00:00

  • Rh(phen)2phi3+ as a shape-selective probe of triple helices.

    abstract::RNA pur*pur-pyr and pyr*pur-pyr (pur = purine, pyr = pyrimidine) triple helices consisting of a Watson-Crick base-paired 28mer hairpin duplex and a Hoogsteen base-paired purine or pyrimidine 12mer are targeted with photoactivated cleavage by the metal complex Rh(phen)2phi3+ (phen = phenanthroline, phi = 9, 10-phenanth...


    pub_type: 杂志文章


    authors: Lim AC,Barton JK

    更新日期:1998-06-23 00:00:00

  • Nuclear matrix localization and specific matrix DNA binding by receptor binding factor 1 of the avian oviduct progesterone receptor.

    abstract::A chromatin acceptor protein for the avian oviduct progesterone receptor (PR), termed receptor binding factor 1 (RBF-1), has recently been shown to (1) be a component of the nuclear binding sites (acceptor sites) for PR and (2) generate high-affinity binding sites (termed the RBF-1 class of sites) on avian genomic DNA...


    pub_type: 杂志文章


    authors: Schuchard M,Subramaniam M,Ruesink T,Spelsberg TC

    更新日期:1991-10-01 00:00:00

  • Structure-function relationship of a recombinant human galactoside-binding protein.

    abstract::A galactoside-binding lectin (hL-31) containing a collagen-like sequence was identified in human tumor cells. It was found to be the homologue of the IgE-binding protein, the macrophage cell-surface Mac-2 antigen, and the murine CBP35, RL-29, and mL-34 lectins. Here we report on the expression in Escherichia coli and ...


    pub_type: 杂志文章


    authors: Ochieng J,Platt D,Tait L,Hogan V,Raz T,Carmi P,Raz A

    更新日期:1993-04-27 00:00:00

  • Identification of residues in CD6 which are critical for ligand binding.

    abstract::CD6 is a member of the scavenger receptor cysteine rich protein superfamily (SRCRSF). This family includes many cell surface proteins whose three-dimensional structures and functions are presently not well understood. The extracellular region of CD6 includes 3 SRCR domains. The membrane proximal SRCR domain specifical...


    pub_type: 杂志文章


    authors: Bodian DL,Skonier JE,Bowen MA,Neubauer M,Siadak AW,Aruffo A,Bajorath J

    更新日期:1997-03-04 00:00:00

  • Correlation of Thermal Stability and Structural Distortion of DNA Interstrand Cross-Links Produced from Oxidized Abasic Sites with Their Selective Formation and Repair.

    abstract::C4'-oxidized (C4-AP) and C5'-oxidized abasic sites (DOB) that are produced following abstraction of a hydrogen atom from the DNA backbone reversibly form cross-links selectively with dA opposite a 3'-adjacent nucleotide, despite the comparable proximity of an opposing dA. A previous report on UvrABC incision of DNA su...


    pub_type: 杂志文章


    authors: Ghosh S,Greenberg MM

    更新日期:2015-10-13 00:00:00

  • Human and murine cytotoxic T lymphocyte serine proteases: subsite mapping with peptide thioester substrates and inhibition of enzyme activity and cytolysis by isocoumarins.

    abstract::The active site structures of human Q31 granzyme A, murine granzymes (A, B, C, D, E, and F), and human granzymes (A, B, and 3) isolated from cytotoxic T lymphocytes (CTL) were studied with peptide thioester substrates, peptide chloromethyl ketone, and isocoumarin inhibitors. Human Q31, murine, and human granzyme A hyd...


    pub_type: 杂志文章


    authors: Odake S,Kam CM,Narasimhan L,Poe M,Blake JT,Krahenbuhl O,Tschopp J,Powers JC

    更新日期:1991-02-26 00:00:00

  • Binding of human fibroblast interferon to concanavalin A-agarose. Involvement of carbohydrate recognition and hydrophobic interaction.

    abstract::Human fibroblast interferon binds to a concanavalin A-agarose (Con A-Sepharose) equilibrated with methyl alpha-D-mannopyranoside, or levan; in contrast, it is only partially retarded on a similar column equilibrated with ethylene glycol. Interferon does not bind, however, to a lectin column equilibrated with both meth...


    pub_type: 杂志文章


    authors: Davey MW,Sulkowski E,Carter WA

    更新日期:1976-02-10 00:00:00

  • Elucidation of the functional metal binding profile of a Cd(II)/Pb(II) sensor CmtR(Sc) from Streptomyces coelicolor.

    abstract::Metal homeostasis and resistance in bacteria is maintained by a panel of metal-sensing transcriptional regulators that collectively control transition metal availability and mediate resistance to heavy metal xenobiotics, including As(III), Cd(II), Pb(II), and Hg(II). The ArsR family constitutes a superfamily of metal ...


    pub_type: 杂志文章


    authors: Wang Y,Kendall J,Cavet JS,Giedroc DP

    更新日期:2010-08-10 00:00:00

  • Ability of DNA and spermidine to affect the activity of restriction endonucleases from several bacterial species.

    abstract::Previous work has described the novel ability to modulate in vitro the activity of restriction endonuclease NaeI from Nocardia aerocoligenes by using cleavable DNA and spermidine [Conrad & Topal (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 9707-9711]. In this paper we report the results of a study of 49 type II restrictio...


    pub_type: 杂志文章


    authors: Oller AR,Vanden Broek W,Conrad M,Topal MD

    更新日期:1991-03-05 00:00:00

  • Fluorescence polarization and intensity kinetic studies of antifluorescein antibody obtained at different stages of the immune response.

    abstract::Kinetic studies of reactions between fluorescein and antifluorescein antibody produced during early, intermediate, and late stages of the immune response have been carried out utilizing both fluorescence intensity and polarization measurements in the static (time constant similar to 5 sec) and in the stopped-flow mode...


    pub_type: 杂志文章


    authors: Levison SA,Hicks AN,Portmann AJ,Dandliker WB

    更新日期:1975-08-26 00:00:00

  • Interaction of a paramagnetic analogue of oxaloacetate with citrate synthase.

    abstract::Electron paramagnetic resonance studies have indicated that nitrosodisulfonate binds to pig heart citrate synthase. Titration of the enzyme with nitrosodisulfonate revealed several binding sites for the probe per subunit with one site (KD approximately 0.1 mM) having a greater affinity than the others. The substrate, ...


    pub_type: 杂志文章


    authors: Weidman SW,Drysdale GR

    更新日期:1979-08-21 00:00:00

  • SmgGDS-607 Regulation of RhoA GTPase Prenylation Is Nucleotide-Dependent.

    abstract::Protein prenylation involves the attachment of a hydrophobic isoprenoid moiety to the C-terminus of proteins. Several small GTPases, including members of the Ras and Rho subfamilies, require prenylation for their normal and pathological functions. Recent work has suggested that SmgGDS proteins regulate the prenylation...


    pub_type: 杂志文章


    authors: Jennings BC,Lawton AJ,Rizk Z,Fierke CA

    更新日期:2018-07-24 00:00:00

  • Calorimetric studies of the interaction of magnesium and phosphate with Na+, K+) ATPase: evidence for a ligand-induced conformational change in the enzyme.

    abstract::The phosphorylation of (Na+, K+)ATPase from the electric organ of the electric eel is dependent on Mg2+. The amount of phosphoenzyme formed was increased by K+ and decreased by Na+. Kinetic analyses indicate that a ternary complex of ATPase, Pi and Mg2+ is formed prior to phosphorylation of the protein. Calorimetric s...


    pub_type: 杂志文章


    authors: Kuriki Y,Halsey J,Biltonen R,Racker E

    更新日期:1976-11-16 00:00:00

  • A proton nuclear magnetic resonance study of the quaternary structure of human homoglobins in water.

    abstract::Proton nuclear magnetic resonance spectra of human hemoglobins in water reveal several exchangeable protons which are indicators of the quaternary structures of both the liganded and unliganded molecules. A comparison of the spectra of normal human adult hemoglobin with those of mutant hemoglobins Chesapeake (FG4alpha...


    pub_type: 杂志文章


    authors: Fung LW,Ho C

    更新日期:1975-06-03 00:00:00

  • Unexpected findings from target analysis of immunoglobulin E and its receptor.

    abstract::The membrane receptor for immunoglobulin E (IgE) and its ligand, IgE, were irradiated with high-energy electrons. Loss of binding activity was measured for each, and the size of the functional targets was assessed. In both cases, the target size was substantially smaller than the covalent structure of the molecule. Th...


    pub_type: 杂志文章


    authors: Fewtrell C,Kempner E,Poy G,Metzger H

    更新日期:1981-11-10 00:00:00

  • Low permeability of liposomal membranes composed of bipolar tetraether lipids from thermoacidophilic archaebacterium Sulfolobus acidocaldarius.

    abstract::The physical origin of the extremely high thermal stability of tetraether liposomes composed of the polar lipid fraction E (PLFE) from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius has been investigated. The leakage rate of trapped 5, 6-carboxyfluorescein (5(6)CF) and the proton permeability in PLFE ...


    pub_type: 杂志文章


    authors: Komatsu H,Chong PL

    更新日期:1998-01-06 00:00:00

  • Flash-induced relaxation changes of the EPR signals from the manganese cluster and YD reveal a light-adaptation process of photosystem II.

    abstract::By exposing photosystem II (PSII) samples to an incrementing number of excitation flashes at room temperature, followed by freezing, we could compare the Mn-derived multiline EPR signal from the S2 oxidation state as prepared by 1, 5, 10, and 25 flashes of light. While the S2 multiline signals exhibited by these sampl...


    pub_type: 杂志文章


    authors: Peterson S,Ahrling KA,Högblom JE,Styring S

    更新日期:2003-03-11 00:00:00

  • Altering the intermediate in the equilibrium folding of unmodified yeast tRNAPhe with monovalent and divalent cations.

    abstract::The isothermal equilibrium folding of the unmodified yeast tRNA(Phe) is studied as a function of Na(+), Mg(2+), and urea concentration with hydroxyl radical protection, circular dichroism, and diethyl pyrocarbonate (DEPC) modification. These assays indicate that this tRNA folds in Na(+) alone. Similar to folding in Mg...


    pub_type: 杂志文章


    authors: Shelton VM,Sosnick TR,Pan T

    更新日期:2001-03-27 00:00:00

  • Covalent structure of collagen: amino acid sequence of alpha 1(III)-CB9 from type III collagen of human liver.

    abstract::The peptide alpha 1(III)-CB9 was prepared and purified from human liver, and its amino acid sequence was determined. Automated Edman degradation of the intact peptide and peptides derived from selective cleavage with hydroxylamine and digestions with trypsin, thermolysin, and Staph V8 protease enabled determination of...


    pub_type: 杂志文章


    authors: Seyer JM,Kang AH

    更新日期:1981-04-28 00:00:00

  • Identification of critical lysyl residues in the pyrophosphate-dependent phosphofructo-1-kinase of Propionibacterium freudenreichii.

    abstract::Pyrophosphate-dependent 6-phosphofructo-1-kinase (PPi-PFK) from Propionibacterium freudenreichii was inactivated by low concentrations of the lysine-specific reagent pyridoxal phosphate (PLP) after sodium borohydride reduction. The substrates fructose 6-phosphate and fructose 1,6-bisphosphate protected against inactiv...


    pub_type: 杂志文章


    authors: Green PC,Latshaw SP,Ladror US,Kemp RG

    更新日期:1992-05-26 00:00:00