Abstract：
:When different mutations were introduced into the anticodon loop and at position 73 of YFA2, a derivative of yeast tRNA(Phe), a single tRNA body was misacylated with 13 different amino acids. The affinities of these misacylated tRNAs for Thermus thermophilus elongation factor Tu (EF-Tu).GTP were determined using a ribonuclease protection assay. A range of 2.5 kcal/mol in the binding energies was observed, clearly demonstrating that EF-Tu specifically recognizes the side chain of the esterified amino acid. Furthermore, this specificity can be altered by introducing a mutation in the amino acid binding pocket on the surface of EF-Tu. Also, when discussed in conjunction with the previously determined specificity of EF-Tu for the tRNA body, these experiments further demonstrate that EF-Tu uses thermodynamic compensation to bind cognate aminoacyl-tRNAs similarly.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Dale T,Sanderson LE,Uhlenbeck OCdoi
10.1021/bi036290osubject
Has Abstractpub_date
2004-05-25 00:00:00pages
6159-66issue
20eissn
0006-2960issn
1520-4995journal_volume
43pub_type
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