Abstract:
:Galactosyl- and glucosylceramide, globoside, and dihydrolactosylceramide, bearing [2,2-2H2]stearic acid, have been studied at a concentration of 10 mol% in bilayers of dimyristoylphosphatidylcholine by 2H NMR. The quadrupolar splitting delta vQ of the C2 deuterons were measured at several temperatures in the range of 30-60 degrees C. Spin-lattice relaxation times T1 of C2 deuterons were determined in the same temperature range for all lipids but globoside. T1 values at 30 and 50 degrees C were unexpectedly short (6-8 ms), indicating reduced mobility of the ceramide acyl chains compared to that of the host phospholipid. At all temperatures, both delta vQ and T1 were essentially identical for the monoglycosylated species, GalCer and GlcCer, indicating that the order and dynamics of the upper portion of the fatty acyl chain are insensitive to this small change in the headgroup structure. In the case of globoside, where the glycolipid headgroup is equivalent to that of GlcCer extended by three sugar residues, values for the quadrupolar splittings associated with the acyl chain C2-position were very close to those obtained for Gal- and GlcCer. In contrast, the delta vQ values obtained for the diglycosyl species, LacCer, were significantly different at all temperatures. This different behavior of LacCer relative to that of the other glycolipids most likely originates from an orientational change of the acyl chain at the C2-position due to the absence of a 4,5 double bond in dihydrosphingosine. T1 values for the GlcCer and GalCer systems increased with temperature, indicating that the motions responsible for relaxation were in the short correlation time regime.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Fenske DB,Hamilton K,Jarrell HC,Florio E,Barber KR,Grant CWdoi
10.1021/bi00232a019subject
Has Abstractpub_date
1991-05-07 00:00:00pages
4503-9issue
18eissn
0006-2960issn
1520-4995journal_volume
30pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The binding modes of four active site-directed, acylating inhibitors of human alpha-thrombin have been determined using X-ray crystallography. These inhibitors (GR157368, GR166081, GR167088, and GR179849) are representatives of a series utilizing a novel 5, 5-trans-lactone template to specifically acylate Ser195 of th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9830359
更新日期:1999-06-22 00:00:00
abstract::A galactoside-binding lectin (hL-31) containing a collagen-like sequence was identified in human tumor cells. It was found to be the homologue of the IgE-binding protein, the macrophage cell-surface Mac-2 antigen, and the murine CBP35, RL-29, and mL-34 lectins. Here we report on the expression in Escherichia coli and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00067a038
更新日期:1993-04-27 00:00:00
abstract::The nuclease hypersensitivity element III(1) (NHE III(1)) of the c-MYC promoter strongly controls the transcriptional activity of the c-MYC oncogene. The purine-rich strand of the NHE III(1) element has been shown to be a silencer element for c-MYC transcription upon formation of a G-quadruplex structure. We have dete...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048242p
更新日期:2005-02-15 00:00:00
abstract::The topography of the photosynthetic reaction center (RC) polypeptides (H, M, and L) was investigated by proteolysis and radioiodination of membrane vesicles isolated from Rhodopseudomonas sphaeroides. Chromatophores, obtained from French-pressed cell lysates, are closed vesicles' and oriented inside out with respect ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00519a012
更新日期:1981-08-04 00:00:00
abstract::Clostridial neurotoxins are zinc endopeptidases, and each contains one Zn(2+)/molecule. To investigate the structural/functional role of Zn(2+) in botulinum neurotoxin light chain (the enzymatic subunit of the neurotoxin), the effect of the removal of zinc on protein folding and enzyme kinetics was investigated. The a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0007472
更新日期:2000-08-29 00:00:00
abstract::FLI1 (Friend leukemia integration 1) is a metazoan transcription factor that is upregulated in a number of cancers. In addition, rearrangements of the fli1 gene cause sarcomas, leukemias, and lymphomas. These rearrangements encode oncogenic transcription factors, in which the DNA binding domain (DBD or ETS domain) of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b01121
更新日期:2015-12-22 00:00:00
abstract::3-(Trifluoromethyl)-3-(m-iodophenyl)diazirine (TID) has recently been found to be a noncompetitive inhibitor of the nicotinic acetylcholine receptor (nAcChoR) by both photolabeling and flux assays (White et al., 1991). However, these experiments were done when TID was in equilibrium with the nAcChoR, and thus only its...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00255a018
更新日期:1994-12-27 00:00:00
abstract::We have shown previously that the hepatic and intestinal transcription of the human apolipoprotein A-II (apoA-II) gene in cell cultures is controlled by a complex set of regulatory elements A-N [Chambaz, et al. (1991) J. Biol. Chem. 266, 11676-11685; Cardot, et al. (1991) J. Biol. Chem. 266, 24460-24470]. In the prese...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00086a013
更新日期:1993-09-07 00:00:00
abstract::The active form of the animal fatty acid synthase (FAS) is a dimer of identical multifunctional polypeptides, each containing seven discrete functional domains, that cooperate to form two centers for palmitate synthesis. To assess the importance of domain cooperation across the subunit interface in the reaction mechan...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971886v
更新日期:1998-02-24 00:00:00
abstract::It has been suggested that dimeric tubulin can participate in the signal transduction process through its association with the GTP-binding (G) proteins Gs and Gi1. Using the photoaffinity GTP analog, azidoanilido-GTP, it has been shown that the transfer of nucleotide from tubulin to G alpha s and G alpha i1 is the key...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00198a052
更新日期:1994-08-16 00:00:00
abstract::The thermodynamic and activation parameters for the reduction of delta 1-pyrroline-2-carboxylic acid (an alpha-imino acid) by reduced nicotinamide adenine dinucleotide phosphate (NADPH) are compared with those for the reduction of the same imino acid by the glutamate dehydrogenase-NADPH complex. The enthalpies of acti...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00341a013
更新日期:1985-09-24 00:00:00
abstract::The far-ultraviolet circular dichroism spectra of bovine and human prothrombin, prothrombin fragment 1, prethrombin 1, prothrombin fragment 2, and prethrombin 2 (prethrombin 2des(1--13)) were determined and the method of Chen et al. [Chen, Y. H., Yang, J. T., & Martinez, H. M. (1972) Biochemistry 11, 4120--4131; Chen,...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00577a017
更新日期:1979-05-15 00:00:00
abstract::The soluble domains of the b and b' subunits of the ATP synthase of the cyanobacterium Synechocystis PCC 6803 were expressed with His tags attached to their N-termini. Following purification, the polypeptides were characterized by chemical cross-linking, analytical ultracentrifugation, and circular dichroism spectrosc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001821j
更新日期:2001-01-09 00:00:00
abstract::A novel inhibitor of p38 mitogen-activated protein kinase (p38), CMPD1, identified by high-throughput screening, is characterized herein. Unlike the p38 inhibitors described previously, this inhibitor is substrate selective and noncompetitive with ATP. In steady-state kinetics experiments, CMPD1 was observed to preven...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0495073
更新日期:2004-09-21 00:00:00
abstract::The substrate-bound form of the enzyme heme oxygenase (HO), which catalyzed the stereospecific alpha-meso bridge cleavage of hemin to yield biliverdin IX alpha, has been investigated by 1H NMR in both its primarily high-spin and its cyanide-inhibited low-spin forms. Both derivatives yield 1H NMR spectra indicative of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00187a033
更新日期:1994-05-31 00:00:00
abstract::DNA ring closure experiments on short restriction fragments ( approximately 160 bp) bound by the TATA box binding protein (TBP) have demonstrated the formation of negative topoisomers, consistent with crystallographically observed TBP-induced DNA untwisting but in contrast to most previous results on topological effec...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi992263f
更新日期:2000-04-04 00:00:00
abstract::Paramagnetic agents produce line broadening and thus cancellation of anti phase cross-peak components in two-dimensional correlated nuclear magnetic resonance spectra. The specificity of this effect was examined to determine its utility for identifying surface residues of proteins. Ubiquitin and hen egg white lysozyme...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00495a005
更新日期:1990-10-30 00:00:00
abstract::Spectroscopic methods are used to probe the interactions of several anilinoacridine analogues with calf thymus DNA over a wide range of temperatures and sodium chloride concentrations. The structurally similar compounds m-AMSA, AMSA (both active as antitumor agents), and o-AMSA (inactive as an antitumor agent) have be...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00231a025
更新日期:1991-04-30 00:00:00
abstract::Oligonucleotides capable of sequence-specific triple helix formation have been proposed as DNA binding ligands useful for modulation of gene expression and for directed genome modification. However, the effectiveness of such triplex-forming oligonucleotides (TFOs) depends on their ability to bind to their target sites...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi982290q
更新日期:1999-02-09 00:00:00
abstract::Ribonuclease P2 from the thermophilic archaebacterium Sulfolobus solfataricus is a small protein (7 kDa) with a known three-dimensional structure. Inspection of the structure and molecular dynamics simulation reveal that three aromatic residues (Phe5, Phe31, and Tyr33) from the hydrophobic core have a strong van der W...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970467v
更新日期:1997-07-22 00:00:00
abstract::Three activities on the pathway of purine biosynthesis de novo in chicken liver, namely, glycinamide ribonucleotide synthetase, glycinamide ribonucleotide transformylase, and aminoimidazole ribonucleotide synthetase, have been found to reside on the same polypeptide chain. Three diverse purification schemes, utilizing...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00346a006
更新日期:1985-12-03 00:00:00
abstract::The effect of Mg2+ on the disposition of myosin cross-bridges was studied on myofibrils and synthetic myosin and rod filaments by employing chymotryptic digestion and chemical cross-linking methods. In the presence of low Mg2+ concentrations (0.1 mM), the proteolytic susceptibility at the heavy meromyosin/light meromy...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00290a012
更新日期:1983-10-11 00:00:00
abstract::Several recent studies have provided evidence that LIN28, a cytoplasmic RNA-binding protein, inhibits the biogenesis of members of the let-7 microRNA family at the Dicer step in both mammals and Caenorhabditis elegans. However, the precise mechanism of inhibition is still poorly understood. Here we report on an in vit...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200851d
更新日期:2011-09-06 00:00:00
abstract::Bioluminescence with luciferase-luciferin pairs is an attractive method for surveying cells in live tissues and whole organisms. Recent advances in luciferin chemistry and luciferase engineering are further expanding the scope of the technology. It is now possible to spy on cells in a variety of deep tissues and visua...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/acs.biochem.7b00435
更新日期:2017-10-03 00:00:00
abstract::A ribonuclease was isolated from serum-free supernatants of the human colon adenocarcinoma cell line HT-29. It was purified by cation-exchange and C18 reversed-phase high-performance liquid chromatography. The protein is basic, has a molecular weight of approximately 16,000, and has an amino acid composition that is s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00371a002
更新日期:1986-11-18 00:00:00
abstract::The interactions of two plant annexins, annexin 24(Ca32) from Capsicum annuum and annexin Gh1 from Gossypium hirsutum, with phospholipid membranes have been characterized using liposome-based assays and adsorption to monolayers. These two plant annexins show a preference for phosphatidylserine-containing membranes and...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0516226
更新日期:2005-12-13 00:00:00
abstract::The observed regioselectivity of the homolytic cleavage of S-adenosylmethionine (SAM) by the radical SAM enzymes is modeled by free radical displacement reactions at sulfoxide centers. These displacements are also regioselective, in direct consequence of the reaction mechanism. The selectivity in the radical SAM react...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi101509u
更新日期:2010-12-28 00:00:00
abstract::The aggregation of the protein tau into amyloid fibrils is known to be involved in the causation of the neurodegenerative tauopathies and the progression of cognitive decline in Alzheimer's disease. This review surveys the mechanism of tau aggregation with special emphasis on the information obtained from biochemical ...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi400209z
更新日期:2013-06-18 00:00:00
abstract::Ribosomal protein E-L30 unfolds reversibly at pH values between 7.0 and 4.5. Unfolding of the protein involves a fast and a slow equilibrium, which depend on the degree of protonation of His19 and His33. Both the fast equilibrium between protonated and deprotonated histidines and the slow equilibrium between folded an...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00391a050
更新日期:1987-08-25 00:00:00
abstract::KCNE1 is a single transmembrane protein that modulates the function of voltage-gated potassium channels, including KCNQ1. Hereditary mutations in the genes encoding either protein can result in diseases such as congenital deafness, long QT syndrome, ventricular tachyarrhythmia, syncope, and sudden cardiac death. Despi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00505
更新日期:2015-10-20 00:00:00